P12747 (LUXI_VIBFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-homoserine-lactone synthase
EC=2.3.1.184

Alternative name(s):
Autoinducer synthesis protein LuxI

Gene names

Name:

luxI

Organism

Vibrio fischeri

Taxonomic identifier

668 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-homoserine lactone) also known as VAI or N-(beta-ketocaproyl)homoserine lactone or 3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-hexanamide, an autoinducer molecule which binds to LuxR and thus acts in bioluminescence regulation.
Catalytic activity	An acyl-[acyl-carrier-protein] + S-adenosyl-L-methionine = [acyl-carrier-protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone.
Sequence similarities	Belongs to the autoinducer synthase family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Luminescence Quorum sensing
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Acyl-homoserine-lactone synthase		PRO_0000210888

Sequences

	Sequence		Length	Mass (Da)
	P12747 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: C97DA69B688E1287	FASTA	193	21,934
	10 20 30 40 50 60 MTIMIKKSDF LAIPSEEYKG ILSLRYQVFK QRLEWDLVVE NNLESDEYDN SNAEYIYACD 70 80 90 100 110 120 DTENVSGCWR LLPTTGDYML KSVFPELLGQ QSAPKDPNIV ELSRFAVGKN SSKINNSASE 130 140 150 160 170 180 ITMKLFEAIY KHAVSQGITE YVTVTSTAIE RFLKRIKVPC HRIGDKEIHV LGDTKSVVLS 190 MPINEQFKKA VLN

References

[1]	"Nucleotide sequence of the luxR and luxI genes and structure of the primary regulatory region of the lux regulon of Vibrio fischeri ATCC 7744." Devine J.H., Countryman C., Baldwin T.O. Biochemistry 27:837-842(1988) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 7744 / NCIMB 1281.
[2]	"Nucleotide sequence of the regulatory locus controlling expression of bacterial genes for bioluminescence." Engebrecht J., Silverman M. Nucleic Acids Res. 15:10455-10467(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ-1.
[3]	"Vibrio fischeri Lux operon SalI digest." Knight T., Papadakis N. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19039 Genomic DNA. Translation: AAA27552.1. Y00509 Genomic DNA. Translation: CAA68562.1. Sequence problems. AF170104 Genomic DNA. Translation: AAD48474.1.
PIR	B28705.
3D structure databases
ProteinModelPortal	P12747.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14564.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001690. Autoind_synth. IPR018311. Autoind_synth_CS. [Graphical view]
Pfam	PF00765. Autoind_synth. 1 hit. [Graphical view]
PRINTS	PR01549. AUTOINDCRSYN.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS00949. AUTOINDUCER_SYNTH_1. 1 hit. PS51187. AUTOINDUCER_SYNTH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXI_VIBFI

Accession

Primary (citable) accession number: P12747

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program