P12746 (LUXR_VIBFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator protein luxR

Gene names

Name:

luxR

Organism

Vibrio fischeri

Taxonomic identifier

668 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Aliivibrio

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator of the bioluminescence operon. Binds to the OHHL autoinducer.
Sequence similarities	Belongs to the autoinducer-regulated transcriptional regulatory protein family. Contains 1 HTH luxR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Luminescence Quorum sensing Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Transcriptional activator protein luxR		PRO_0000184161
Regions
	Domain	176 – 241	66	HTH luxR-type
	DNA binding	200 – 219	20	H-T-H motif Potential
	Compositional bias	11 – 20	10	Arg/Lys-rich (basic)
	Compositional bias	64 – 67	4	Arg/Lys-rich (basic)
	Compositional bias	104 – 114	11	Lys-rich (basic)
Natural variations
	Natural variant	3	1	N → D in strain: ATCC 7744.
	Natural variant	115	1	T → S in strain: ATCC 7744.
	Natural variant	211	1	E → K in strain: ATCC 7744.
	Natural variant	250	1	N → S in strain: ATCC 7744.
Experimental info
	Sequence conflict	21 – 23	3	SNN → AY in CAA68561. Ref.3
	Sequence conflict	42	1	L → LT in CAA68561. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P12746 [UniParc]. Last modified February 1, 1994. Version 3. Checksum: BDB636192F5E1C64	FASTA	250	28,556
	10 20 30 40 50 60 MKNINADDTY RIINKIKACR SNNDINQCLS DMTKMVHCEY YLLAIIYPHS MVKSDISILD 70 80 90 100 110 120 NYPKKWRQYY DDANLIKYDP IVDYSNSNHS PINWNIFENN AVNKKSPNVI KEAKTSGLIT 130 140 150 160 170 180 GFSFPIHTAN NGFGMLSFAH SEKDNYIDSL FLHACMNIPL IVPSLVDNYR KINIANNKSN 190 200 210 220 230 240 NDLTKREKEC LAWACEGKSS WDISKILGCS ERTVTFHLTN AQMKLNTTNR CQSISKAILT 250 GAIDCPYFKN

References

[1]	"Nucleotide sequence of the luxR and luxI genes and structure of the primary regulatory region of the lux regulon of Vibrio fischeri ATCC 7744." Devine J.H., Countryman C., Baldwin T.O. Biochemistry 27:837-842(1988) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 7744 / NCIMB 1281.
[2]	"Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744." Devine J.H., Shadel G.S., Baldwin T.O. Proc. Natl. Acad. Sci. U.S.A. 86:5688-5692(1989) [PubMed: 2762291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION. Strain: ATCC 7744 / NCIMB 1281.
[3]	"Nucleotide sequence of the regulatory locus controlling expression of bacterial genes for bioluminescence." Engebrecht J., Silverman M. Nucleic Acids Res. 15:10455-10467(1987) [PubMed: 3697093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ-1.
[4]	"Vibrio fischeri Lux operon SalI digest." Knight T., Papadakis N. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ-1.
[5]	"Overproduction and purification of the luxR gene product: transcriptional activator of the Vibrio fischeri luminescence system." Kaplan H.B., Greenberg E.P. Proc. Natl. Acad. Sci. U.S.A. 84:6639-6643(1987) [PubMed: 16578817] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15, DNA-BINDING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25751 Genomic DNA. Translation: AAA27540.1. M19039 Genomic DNA. Translation: AAA27551.1. M25752 Genomic DNA. Translation: AAA27541.1. AF170104 Genomic DNA. Translation: AAD48473.1. Y00509 Genomic DNA. Translation: CAA68561.1.
PIR	B33538. S06314.
3D structure databases
ProteinModelPortal	P12746.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016032. Sig_transdc_resp-reg_C-effctor. IPR005143. TF_LuxR_autoind-bd_dom. IPR000792. Tscrpt_reg_LuxR_C. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.450.80. TF_LuxR_autoind-bd_dom. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF03472. Autoind_bind. 1 hit. PF00196. GerE. 1 hit. [Graphical view]
PRINTS	PR00038. HTHLUXR.
SMART	SM00421. HTH_LUXR. 1 hit. [Graphical view]
SUPFAM	SSF46894. Bipartite_resp_reg_C-effector. 1 hit. SSF75516. TF_LuxR_autoind-bd_dom. 1 hit.
PROSITE	PS00622. HTH_LUXR_1. 1 hit. PS50043. HTH_LUXR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXR_VIBFI

Accession

Primary (citable) accession number: P12746

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program