Reviewed, UniProtKB/Swiss-Prot P12745 (LUXF_PHOPO)

Names and origin

Protein names

Recommended name:
    Non-fluorescent flavoprotein
      Short name=NFP
Alternative name(s):
    FP390

Gene names

Name:

luxF

Organism

Photobacterium phosphoreum

Taxonomic identifier

659 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 2 FMN per subunit. Each FMN has a myristate attached.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	Flavoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 231

231

Non-fluorescent flavoprotein

PRO_0000220183

Secondary structure

1

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231

Helix Strand Turn

Details...

Beta strand

6 – 11

6

Helix

23 – 38

16

Beta strand

40 – 48

9

Beta strand

65 – 68

4

Beta strand

71 – 75

5

Helix

80 – 87

8

Beta strand

92 – 94

3

Beta strand

96 – 98

3

Helix

100 – 116

17

Beta strand

126 – 130

5

Helix

138 – 155

18

Helix

166 – 171

6

Beta strand

174 – 177

4

Helix

178 – 190

13

Turn

191 – 193

3

Beta strand

195 – 199

5

Helix

207 – 227

21

Sequences

	Sequence		Length	Mass (Da)
	P12745-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 87EB6482C2870661	FASTA	231	26,606
	10 20 30 40 50 60 MNKWNYGVFF VNFYNKGQQE PSKTMNNALE TLRIIDEDTS IYDVINIDDH YLVKKDSEDK 70 80 90 100 110 120 KLASFITLGE KLYVLATSEN TVDIAAKYAL PLVFKWDDIN EERLKLLSFY NASASKYNKN 130 140 150 160 170 180 IDLVRHQLML HVNVNEAETV AKEELKLYIE NYVACTQPSN FNGSIDSIIQ SNVTGSYKDC 190 200 210 220 230 LSYVANLAGK FDNTVDFLLC FESMQDQNKK KSVMIDLNNQ VIKFRQDNNL I

References

[1]	"A new lux gene in bioluminescent bacteria codes for a protein homologous to the bacterial luciferase subunits." Soly R.R., Mancini J.A., Ferri S.R., Boylan M., Meighen E.A. Biochem. Biophys. Res. Commun. 155:351-358(1988) [PubMed: 3415691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and properties of luciferase from Photobacterium phosphoreum." Ferri S.R., Soly R.R., Szittner R.B., Meighen E.A. Biochem. Biophys. Res. Commun. 176:541-548(1991) [PubMed: 2018544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
[3]	"Identification of the acyl transfer site of fatty acyl-protein synthetase from bioluminescent bacteria." Soly R.R., Meighen E.A. J. Mol. Biol. 219:69-77(1991) [PubMed: 2023262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 218-231.
[4]	"Structure of flavoprotein FP390 from a luminescent bacterium Photobacterium phosphoreum refined at 2.7-A resolution." Kita A., Kasai S., Miyata M., Miki K. Acta Crystallogr. D 52:77-86(1996) [PubMed: 15299728] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

M22128 Genomic DNA. Translation: AAA25623.1.
M65067 Genomic DNA. Translation: AAA70299.1.

PIR

A31572.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FVP	X-ray	2.70	A/B	1-231	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like.
IPR016048. Luciferase-like_sub.
IPR002103. Luciferase_bac.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

Pfam

PF00296. Bac_luciferase. 1 hit.
[Graphical view]

PRINTS

PR00089. LUCIFERASE.

PROSITE

PS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LUXF_PHOPO

Accession

Primary (citable) accession number: P12745

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)