Reviewed, UniProtKB/Swiss-Prot P12744 (LUXB_PHOPO)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase beta chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Photobacterium phosphoreum

Taxonomic identifier

659 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O(2) = RCOOH + FMN + H(2)O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Alkanal monooxygenase beta chain		PRO_0000220179
Experimental info
	Sequence conflict	307	1	M → MV in AAA25622. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12744-1 [UniParc]. Last modified March 1, 1992. Version 2. Checksum: 9ECAE7C176A72C04	FASTA	318	36,397
	10 20 30 40 50 60 MNFGLFFLNF PENTSSETVL DNMINTVSLV DKDYKNFTTA LVNHHFSKNG IVGAPMTAAS 70 80 90 100 110 120 FLLGLTERLH IGSLNQITTH HPVRIAEEAS LLDQMSDSRF ILGLSDCVNF EMDFFKRQRD 130 140 150 160 170 180 SQQLQFEACY DIINEAITTN YCANNDFYNF PRISINPHCL SKENMKQYIL ASSVSVEWAA 190 200 210 220 230 240 KKALPLTYRW SDTLEDKEIL YKRYLEVAKH NIDVSNVEHQ FPLLVNLNHD RDVAHQEATA 250 260 270 280 290 300 YVSYIAEVYP HLNQQQKIAE LISQHAIGTD NDYYSTLNAL ERTGSKNVLL SFESMKNHDD 310 VVKVINMNEK IQKNLPSS

References

[1]	"Structure and properties of luciferase from Photobacterium phosphoreum." Ferri S.R., Soly R.R., Szittner R.B., Meighen E.A. Biochem. Biophys. Res. Commun. 176:541-548(1991) [PubMed: 2018544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A new lux gene in bioluminescent bacteria codes for a protein homologous to the bacterial luciferase subunits." Soly R.R., Mancini J.A., Ferri S.R., Boylan M., Meighen E.A. Biochem. Biophys. Res. Commun. 155:351-358(1988) [PubMed: 3415691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 301-318.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65067 Genomic DNA. Translation: AAA70298.1. M22128 Genomic DNA. Translation: AAA25622.1.
PIR	JH0388.
3D structure databases
HSSP	HSSP built from PDB template 1BSL based on UniProtKB P07739.
ModBase	Search...
Family and domain databases
InterPro	IPR011251. Luciferase-like_bac. IPR002103. Luciferase_bac. IPR016048. Luciferase_mOase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB_PHOPO

Accession

Primary (citable) accession number: P12744

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	March 1, 1992
Last modified:	November 25, 2008
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)