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Protein

Alkanal monooxygenase beta chain

Gene

luxB

Organism
Photobacterium phosphoreum
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.1 Publication

Catalytic activityi

A long-chain aldehyde + FMNH2 + O2 = a long-chain fatty acid + FMN + H2O + light.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase, Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Flavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
Alkanal monooxygenase beta chain (EC:1.14.14.31 Publication)
Alternative name(s):
Bacterial luciferase beta chain
Gene namesi
Name:luxB
OrganismiPhotobacterium phosphoreum
Taxonomic identifieri659 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaePhotobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002201791 – 318Alkanal monooxygenase beta chainAdd BLAST318

Proteomic databases

PRIDEiP12744.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.By similarity

Structurei

3D structure databases

ProteinModelPortaliP12744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd01096. Alkanal_monooxygenase. 1 hit.
Gene3Di3.20.20.30. 2 hits.
InterProiIPR033924. Alkanal_monooxygenase.
IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac/NFP.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFGLFFLNF PENTSSETVL DNMINTVSLV DKDYKNFTTA LVNHHFSKNG
60 70 80 90 100
IVGAPMTAAS FLLGLTERLH IGSLNQITTH HPVRIAEEAS LLDQMSDSRF
110 120 130 140 150
ILGLSDCVNF EMDFFKRQRD SQQLQFEACY DIINEAITTN YCANNDFYNF
160 170 180 190 200
PRISINPHCL SKENMKQYIL ASSVSVEWAA KKALPLTYRW SDTLEDKEIL
210 220 230 240 250
YKRYLEVAKH NIDVSNVEHQ FPLLVNLNHD RDVAHQEATA YVSYIAEVYP
260 270 280 290 300
HLNQQQKIAE LISQHAIGTD NDYYSTLNAL ERTGSKNVLL SFESMKNHDD
310
VVKVINMNEK IQKNLPSS
Length:318
Mass (Da):36,397
Last modified:March 1, 1992 - v2
Checksum:i9ECAE7C176A72C04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti307M → MV in AAA25622 (PubMed:3415691).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65067 Genomic DNA. Translation: AAA70298.1.
M22128 Genomic DNA. Translation: AAA25622.1.
PIRiJH0388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65067 Genomic DNA. Translation: AAA70298.1.
M22128 Genomic DNA. Translation: AAA25622.1.
PIRiJH0388.

3D structure databases

ProteinModelPortaliP12744.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP12744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd01096. Alkanal_monooxygenase. 1 hit.
Gene3Di3.20.20.30. 2 hits.
InterProiIPR033924. Alkanal_monooxygenase.
IPR018235. Bacterial_luciferase_CS.
IPR011251. Luciferase-like_dom.
IPR002103. Luciferase_bac/NFP.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
PRINTSiPR00089. LUCIFERASE.
SUPFAMiSSF51679. SSF51679. 1 hit.
PROSITEiPS00494. BACTERIAL_LUCIFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUXB_PHOPO
AccessioniPrimary (citable) accession number: P12744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.