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Protein

50S ribosomal protein L7Ae

Gene

rpl7ae

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-95-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L7AeUniRule annotation
Alternative name(s):
Hs6
Ribosomal protein L8eUniRule annotation
Gene namesi
Name:rpl7aeUniRule annotation
Ordered Locus Names:rrnAC0103
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 12011950S ribosomal protein L7AePRO_0000136790Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Interacts with protein L15e. Probably part of the RNase P complex.2 Publications

Protein-protein interaction databases

STRINGi272569.rrnAC0103.

Structurei

Secondary structure

1
120
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2616Combined sources
Beta strandi30 – 334Combined sources
Helixi34 – 4310Combined sources
Beta strandi49 – 535Combined sources
Helixi58 – 603Combined sources
Turni61 – 633Combined sources
Helixi64 – 696Combined sources
Turni70 – 723Combined sources
Beta strandi75 – 795Combined sources
Helixi81 – 877Combined sources
Beta strandi95 – 1017Combined sources
Helixi106 – 11813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFKX-ray2.40E2-120[»]
1JJ2X-ray2.40F2-120[»]
1K73X-ray3.01H2-120[»]
1K8AX-ray3.00H2-120[»]
1K9MX-ray3.00H2-120[»]
1KC8X-ray3.01H2-120[»]
1KD1X-ray3.00H2-120[»]
1KQSX-ray3.10F2-120[»]
1M1KX-ray3.20H2-120[»]
1M90X-ray2.80H2-120[»]
1N8RX-ray3.00H2-120[»]
1NJIX-ray3.00H2-120[»]
1Q7YX-ray3.20H2-120[»]
1Q81X-ray2.95H2-120[»]
1Q82X-ray2.98H2-120[»]
1Q86X-ray3.00H2-120[»]
1QVFX-ray3.10F2-120[»]
1QVGX-ray2.90F2-120[»]
1S72X-ray2.40F1-120[»]
1VQ4X-ray2.70F1-120[»]
1VQ5X-ray2.60F1-120[»]
1VQ6X-ray2.70F1-120[»]
1VQ7X-ray2.50F1-120[»]
1VQ8X-ray2.20F1-120[»]
1VQ9X-ray2.40F1-120[»]
1VQKX-ray2.30F1-120[»]
1VQLX-ray2.30F1-120[»]
1VQMX-ray2.30F1-120[»]
1VQNX-ray2.40F1-120[»]
1VQOX-ray2.20F1-120[»]
1VQPX-ray2.25F1-120[»]
1W2BX-ray3.50F2-120[»]
1YHQX-ray2.40F1-120[»]
1YI2X-ray2.65F1-120[»]
1YIJX-ray2.60F1-120[»]
1YITX-ray2.80F1-120[»]
1YJ9X-ray2.90F1-120[»]
1YJNX-ray3.00F1-120[»]
1YJWX-ray2.90F1-120[»]
2OTJX-ray2.90F1-120[»]
2OTLX-ray2.70F1-120[»]
2QA4X-ray3.00F1-120[»]
2QEXX-ray2.90F1-120[»]
3CC2X-ray2.40F1-120[»]
3CC4X-ray2.70F1-120[»]
3CC7X-ray2.70F1-120[»]
3CCEX-ray2.75F1-120[»]
3CCJX-ray2.70F1-120[»]
3CCLX-ray2.90F1-120[»]
3CCMX-ray2.55F1-120[»]
3CCQX-ray2.90F1-120[»]
3CCRX-ray3.00F1-120[»]
3CCSX-ray2.95F1-120[»]
3CCUX-ray2.80F1-120[»]
3CCVX-ray2.90F1-120[»]
3CD6X-ray2.75F1-120[»]
3CMAX-ray2.80F1-120[»]
3CMEX-ray2.95F1-120[»]
3CPWX-ray2.70F1-120[»]
3CXCX-ray3.00F2-120[»]
3G4SX-ray3.20F2-120[»]
3G6EX-ray2.70F2-120[»]
3G71X-ray2.85F2-120[»]
3I55X-ray3.11F1-120[»]
3I56X-ray2.90F1-120[»]
3OW2X-ray2.70F2-120[»]
4ADXelectron microscopy6.60F1-120[»]
4V9FX-ray2.40F1-120[»]
ProteinModelPortaliP12743.
SMRiP12743. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12743.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
KOiK02936.
OMAiFIETQDD.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. eL8_ribo. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVYVDFDVP ADLEDDALEA LEVARDTGAV KKGTNETTKS IERGSAELVF
60 70 80 90 100
VAEDVQPEEI VMHIPELADE KGVPFIFVEQ QDDLGHAAGL EVGSAAAAVT
110 120
DAGEADADVE DIADKVEELR
Length:120
Mass (Da):12,782
Last modified:January 23, 2007 - v4
Checksum:i96132F5CB51840A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1118EADADVED → AAATV AA sequence (PubMed:3315748).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596297 Genomic DNA. Translation: AAV45179.1.
PIRiS00182. R5HSS6.
RefSeqiWP_004593501.1. NC_006396.1.
YP_134885.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV45179; AAV45179; rrnAC0103.
GeneIDi23806048.
3130242.
KEGGihma:rrnAC0103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY596297 Genomic DNA. Translation: AAV45179.1.
PIRiS00182. R5HSS6.
RefSeqiWP_004593501.1. NC_006396.1.
YP_134885.1. NC_006396.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFKX-ray2.40E2-120[»]
1JJ2X-ray2.40F2-120[»]
1K73X-ray3.01H2-120[»]
1K8AX-ray3.00H2-120[»]
1K9MX-ray3.00H2-120[»]
1KC8X-ray3.01H2-120[»]
1KD1X-ray3.00H2-120[»]
1KQSX-ray3.10F2-120[»]
1M1KX-ray3.20H2-120[»]
1M90X-ray2.80H2-120[»]
1N8RX-ray3.00H2-120[»]
1NJIX-ray3.00H2-120[»]
1Q7YX-ray3.20H2-120[»]
1Q81X-ray2.95H2-120[»]
1Q82X-ray2.98H2-120[»]
1Q86X-ray3.00H2-120[»]
1QVFX-ray3.10F2-120[»]
1QVGX-ray2.90F2-120[»]
1S72X-ray2.40F1-120[»]
1VQ4X-ray2.70F1-120[»]
1VQ5X-ray2.60F1-120[»]
1VQ6X-ray2.70F1-120[»]
1VQ7X-ray2.50F1-120[»]
1VQ8X-ray2.20F1-120[»]
1VQ9X-ray2.40F1-120[»]
1VQKX-ray2.30F1-120[»]
1VQLX-ray2.30F1-120[»]
1VQMX-ray2.30F1-120[»]
1VQNX-ray2.40F1-120[»]
1VQOX-ray2.20F1-120[»]
1VQPX-ray2.25F1-120[»]
1W2BX-ray3.50F2-120[»]
1YHQX-ray2.40F1-120[»]
1YI2X-ray2.65F1-120[»]
1YIJX-ray2.60F1-120[»]
1YITX-ray2.80F1-120[»]
1YJ9X-ray2.90F1-120[»]
1YJNX-ray3.00F1-120[»]
1YJWX-ray2.90F1-120[»]
2OTJX-ray2.90F1-120[»]
2OTLX-ray2.70F1-120[»]
2QA4X-ray3.00F1-120[»]
2QEXX-ray2.90F1-120[»]
3CC2X-ray2.40F1-120[»]
3CC4X-ray2.70F1-120[»]
3CC7X-ray2.70F1-120[»]
3CCEX-ray2.75F1-120[»]
3CCJX-ray2.70F1-120[»]
3CCLX-ray2.90F1-120[»]
3CCMX-ray2.55F1-120[»]
3CCQX-ray2.90F1-120[»]
3CCRX-ray3.00F1-120[»]
3CCSX-ray2.95F1-120[»]
3CCUX-ray2.80F1-120[»]
3CCVX-ray2.90F1-120[»]
3CD6X-ray2.75F1-120[»]
3CMAX-ray2.80F1-120[»]
3CMEX-ray2.95F1-120[»]
3CPWX-ray2.70F1-120[»]
3CXCX-ray3.00F2-120[»]
3G4SX-ray3.20F2-120[»]
3G6EX-ray2.70F2-120[»]
3G71X-ray2.85F2-120[»]
3I55X-ray3.11F1-120[»]
3I56X-ray2.90F1-120[»]
3OW2X-ray2.70F2-120[»]
4ADXelectron microscopy6.60F1-120[»]
4V9FX-ray2.40F1-120[»]
ProteinModelPortaliP12743.
SMRiP12743. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272569.rrnAC0103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV45179; AAV45179; rrnAC0103.
GeneIDi23806048.
3130242.
KEGGihma:rrnAC0103.

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
KOiK02936.
OMAiFIETQDD.

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-95-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP12743.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. eL8_ribo. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  2. "Primary structures of three highly acidic ribosomal proteins S6, S12 and S15 from the archaebacterium Halobacterium marismortui."
    Kimura J., Arndt E., Kimura M.
    FEBS Lett. 224:65-70(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-120.
  3. "The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
    Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
    Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  4. "The structural basis of ribosome activity in peptide bond synthesis."
    Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
    Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  5. "A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
    Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
    Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  6. "The kink-turn: a new RNA secondary structure motif."
    Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
    EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  7. "The structures of four macrolide antibiotics bound to the large ribosomal subunit."
    Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
    Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  9. "Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
    Hansen J.L., Moore P.B., Steitz T.A.
    J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  10. "Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
    Schmeing T.M., Moore P.B., Steitz T.A.
    RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
  11. "Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
    Gabdulkhakov A., Nikonov S., Garber M.
    Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.

Entry informationi

Entry nameiRL7A_HALMA
AccessioniPrimary (citable) accession number: P12743
Secondary accession number(s): Q5V5M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 128 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a protein from the small subunit of the ribosome.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.