SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P12736

- RL32_HALMA

UniProt

P12736 - RL32_HALMA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
50S ribosomal protein L32e
Gene
rpl32e, rrnAC1595
Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the 23S rRNA.UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. nucleotide binding Source: InterPro
  3. rRNA binding Source: UniProtKB-KW
  4. structural constituent of ribosome Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: InterPro
  2. translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciHMAR272569:GJDH-1451-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L32e
Alternative name(s):
Hl5
Gene namesi
Name:rpl32e
Ordered Locus Names:rrnAC1595
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
ProteomesiUP000001169: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. ribosome Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 24124050S ribosomal protein L32eUniRule annotation
PRO_0000131148Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Interacts weakly with protein L15.8 Publications

Protein-protein interaction databases

STRINGi272569.rrnAC1595.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi97 – 1004
Helixi112 – 12413
Helixi134 – 1363
Beta strandi138 – 1403
Turni153 – 1575
Helixi167 – 1693
Turni173 – 1775
Beta strandi184 – 1907
Helixi191 – 1944
Turni199 – 2013
Beta strandi202 – 2065
Beta strandi208 – 2103
Helixi212 – 22413
Beta strandi229 – 2313
Beta strandi234 – 2374

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FFKX-ray2.40V99-241[»]
1JJ2X-ray2.40X2-241[»]
1K73X-ray3.01Z2-241[»]
1K8AX-ray3.00Z2-241[»]
1K9MX-ray3.00Z2-241[»]
1KC8X-ray3.01Z2-241[»]
1KD1X-ray3.00Z2-241[»]
1KQSX-ray3.10X2-241[»]
1M1KX-ray3.20Z2-241[»]
1M90X-ray2.80Z2-241[»]
1N8RX-ray3.00Z2-241[»]
1NJIX-ray3.00Z2-241[»]
1Q7YX-ray3.20Z2-241[»]
1Q81X-ray2.95Z2-241[»]
1Q82X-ray2.98Z2-241[»]
1Q86X-ray3.00Z2-241[»]
1QVFX-ray3.10X2-241[»]
1QVGX-ray2.90X2-241[»]
1S72X-ray2.40Y1-241[»]
1VQ4X-ray2.70Y1-241[»]
1VQ5X-ray2.60Y1-241[»]
1VQ6X-ray2.70Y1-241[»]
1VQ7X-ray2.50Y1-241[»]
1VQ8X-ray2.20Y1-241[»]
1VQ9X-ray2.40Y1-241[»]
1VQKX-ray2.30Y1-241[»]
1VQLX-ray2.30Y1-241[»]
1VQMX-ray2.30Y1-241[»]
1VQNX-ray2.40Y1-241[»]
1VQOX-ray2.20Y1-241[»]
1VQPX-ray2.25Y1-241[»]
1W2BX-ray3.50X2-241[»]
1YHQX-ray2.40Y1-241[»]
1YI2X-ray2.65Y1-241[»]
1YIJX-ray2.60Y1-241[»]
1YITX-ray2.80Y1-241[»]
1YJ9X-ray2.90Y1-241[»]
1YJNX-ray3.00Y1-241[»]
1YJWX-ray2.90Y1-241[»]
2OTJX-ray2.90Y1-241[»]
2OTLX-ray2.70Y1-241[»]
2QA4X-ray3.00Y1-241[»]
2QEXX-ray2.90Y1-241[»]
3CC2X-ray2.40Y1-241[»]
3CC4X-ray2.70Y1-241[»]
3CC7X-ray2.70Y1-241[»]
3CCEX-ray2.75Y1-241[»]
3CCJX-ray2.70Y1-241[»]
3CCLX-ray2.90Y1-241[»]
3CCMX-ray2.55Y1-241[»]
3CCQX-ray2.90Y1-241[»]
3CCRX-ray3.00Y1-241[»]
3CCSX-ray2.95Y1-241[»]
3CCUX-ray2.80Y1-241[»]
3CCVX-ray2.90Y1-241[»]
3CD6X-ray2.75Y1-241[»]
3CMAX-ray2.80Y1-240[»]
3CMEX-ray2.95Y1-240[»]
3CPWX-ray2.70X1-241[»]
3CXCX-ray3.00X2-241[»]
3G4SX-ray3.20Y96-237[»]
3G6EX-ray2.70Y96-237[»]
3G71X-ray2.85Y96-237[»]
3I55X-ray3.11Y1-241[»]
3I56X-ray2.90Y1-241[»]
3OW2X-ray2.70X96-237[»]
4ADXelectron microscopy6.60Y1-241[»]
4HUBX-ray2.40Y1-241[»]
ProteinModelPortaliP12736.
SMRiP12736. Positions 96-237.

Miscellaneous databases

EvolutionaryTraceiP12736.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1717.
HOGENOMiHOG000038489.
KOiK02912.
OMAiKPQFNRQ.

Family and domain databases

HAMAPiMF_00810. Ribosomal_L32e.
InterProiIPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR001515. Ribosomal_L32e.
IPR023654. Ribosomal_L32e_arc.
IPR018263. Ribosomal_L32e_CS.
[Graphical view]
PfamiPF01655. Ribosomal_L32e. 1 hit.
[Graphical view]
ProDomiPD003823. Ribosomal_L32e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52042. SSF52042. 1 hit.
PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12736-1 [UniParc]FASTAAdd to Basket

« Hide

MADNEEDVEA EEEYTELTDI SGVGPSKAES LREAGFESVE DVRGADQSAL    50
ADVSGIGNAL AARIKADVGG LEVESETEAE VEEEGGEEAP DEDVETELQA 100
RGLTEKTPDL SDEDARLLTQ RHRVGKPQFN RQDHHKKKRV STSWRKPRGQ 150
LSKQRRGIKG KGDTVEAGFR SPTAVRGKHP SGFEEVRVHN VDDLEGVDGD 200
TEAVRIASKV GARKRERIEE EAEDAGIRVL NPTYVEVEVS E 241
Length:241
Mass (Da):26,416
Last modified:January 23, 2007 - v3
Checksum:iF4FFF58DE34B3711
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261S → R AA sequence 1 Publication
Sequence conflicti31 – 311Missing AA sequence 1 Publication
Sequence conflicti110 – 1112LS → SL AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58395 Genomic DNA. Translation: CAA41288.1.
AY596297 Genomic DNA. Translation: AAV46513.1.
PIRiS16539. R7HSH5.
RefSeqiWP_011223739.1. NC_006396.1.
YP_136219.1. NC_006396.1.

Genome annotation databases

EnsemblBacteriaiAAV46513; AAV46513; rrnAC1595.
GeneIDi3128514.
KEGGihma:rrnAC1595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58395 Genomic DNA. Translation: CAA41288.1 .
AY596297 Genomic DNA. Translation: AAV46513.1 .
PIRi S16539. R7HSH5.
RefSeqi WP_011223739.1. NC_006396.1.
YP_136219.1. NC_006396.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FFK X-ray 2.40 V 99-241 [» ]
1JJ2 X-ray 2.40 X 2-241 [» ]
1K73 X-ray 3.01 Z 2-241 [» ]
1K8A X-ray 3.00 Z 2-241 [» ]
1K9M X-ray 3.00 Z 2-241 [» ]
1KC8 X-ray 3.01 Z 2-241 [» ]
1KD1 X-ray 3.00 Z 2-241 [» ]
1KQS X-ray 3.10 X 2-241 [» ]
1M1K X-ray 3.20 Z 2-241 [» ]
1M90 X-ray 2.80 Z 2-241 [» ]
1N8R X-ray 3.00 Z 2-241 [» ]
1NJI X-ray 3.00 Z 2-241 [» ]
1Q7Y X-ray 3.20 Z 2-241 [» ]
1Q81 X-ray 2.95 Z 2-241 [» ]
1Q82 X-ray 2.98 Z 2-241 [» ]
1Q86 X-ray 3.00 Z 2-241 [» ]
1QVF X-ray 3.10 X 2-241 [» ]
1QVG X-ray 2.90 X 2-241 [» ]
1S72 X-ray 2.40 Y 1-241 [» ]
1VQ4 X-ray 2.70 Y 1-241 [» ]
1VQ5 X-ray 2.60 Y 1-241 [» ]
1VQ6 X-ray 2.70 Y 1-241 [» ]
1VQ7 X-ray 2.50 Y 1-241 [» ]
1VQ8 X-ray 2.20 Y 1-241 [» ]
1VQ9 X-ray 2.40 Y 1-241 [» ]
1VQK X-ray 2.30 Y 1-241 [» ]
1VQL X-ray 2.30 Y 1-241 [» ]
1VQM X-ray 2.30 Y 1-241 [» ]
1VQN X-ray 2.40 Y 1-241 [» ]
1VQO X-ray 2.20 Y 1-241 [» ]
1VQP X-ray 2.25 Y 1-241 [» ]
1W2B X-ray 3.50 X 2-241 [» ]
1YHQ X-ray 2.40 Y 1-241 [» ]
1YI2 X-ray 2.65 Y 1-241 [» ]
1YIJ X-ray 2.60 Y 1-241 [» ]
1YIT X-ray 2.80 Y 1-241 [» ]
1YJ9 X-ray 2.90 Y 1-241 [» ]
1YJN X-ray 3.00 Y 1-241 [» ]
1YJW X-ray 2.90 Y 1-241 [» ]
2OTJ X-ray 2.90 Y 1-241 [» ]
2OTL X-ray 2.70 Y 1-241 [» ]
2QA4 X-ray 3.00 Y 1-241 [» ]
2QEX X-ray 2.90 Y 1-241 [» ]
3CC2 X-ray 2.40 Y 1-241 [» ]
3CC4 X-ray 2.70 Y 1-241 [» ]
3CC7 X-ray 2.70 Y 1-241 [» ]
3CCE X-ray 2.75 Y 1-241 [» ]
3CCJ X-ray 2.70 Y 1-241 [» ]
3CCL X-ray 2.90 Y 1-241 [» ]
3CCM X-ray 2.55 Y 1-241 [» ]
3CCQ X-ray 2.90 Y 1-241 [» ]
3CCR X-ray 3.00 Y 1-241 [» ]
3CCS X-ray 2.95 Y 1-241 [» ]
3CCU X-ray 2.80 Y 1-241 [» ]
3CCV X-ray 2.90 Y 1-241 [» ]
3CD6 X-ray 2.75 Y 1-241 [» ]
3CMA X-ray 2.80 Y 1-240 [» ]
3CME X-ray 2.95 Y 1-240 [» ]
3CPW X-ray 2.70 X 1-241 [» ]
3CXC X-ray 3.00 X 2-241 [» ]
3G4S X-ray 3.20 Y 96-237 [» ]
3G6E X-ray 2.70 Y 96-237 [» ]
3G71 X-ray 2.85 Y 96-237 [» ]
3I55 X-ray 3.11 Y 1-241 [» ]
3I56 X-ray 2.90 Y 1-241 [» ]
3OW2 X-ray 2.70 X 96-237 [» ]
4ADX electron microscopy 6.60 Y 1-241 [» ]
4HUB X-ray 2.40 Y 1-241 [» ]
ProteinModelPortali P12736.
SMRi P12736. Positions 96-237.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272569.rrnAC1595.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV46513 ; AAV46513 ; rrnAC1595 .
GeneIDi 3128514.
KEGGi hma:rrnAC1595.

Phylogenomic databases

eggNOGi COG1717.
HOGENOMi HOG000038489.
KOi K02912.
OMAi KPQFNRQ.

Enzyme and pathway databases

BioCyci HMAR272569:GJDH-1451-MONOMER.

Miscellaneous databases

EvolutionaryTracei P12736.

Family and domain databases

HAMAPi MF_00810. Ribosomal_L32e.
InterProi IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR001515. Ribosomal_L32e.
IPR023654. Ribosomal_L32e_arc.
IPR018263. Ribosomal_L32e_CS.
[Graphical view ]
Pfami PF01655. Ribosomal_L32e. 1 hit.
[Graphical view ]
ProDomi PD003823. Ribosomal_L32e. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00278. HhH1. 2 hits.
[Graphical view ]
SUPFAMi SSF47794. SSF47794. 1 hit.
SSF52042. SSF52042. 1 hit.
PROSITEi PS00580. RIBOSOMAL_L32E. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization and nucleotide sequence of ten ribosomal protein genes from the region equivalent to the spectinomycin operon in the archaebacterium Halobacterium marismortui."
    Scholzen T., Arndt E.
    Mol. Gen. Genet. 228:70-80(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  3. "Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane."
    Walsh M.J., McDougall J., Wittmann-Liebold B.
    Biochemistry 27:6867-6876(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-32 AND 98-113.
  4. "The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
    Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
    Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 99-241.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  5. "The structural basis of ribosome activity in peptide bond synthesis."
    Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
    Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  6. "A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
    Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
    Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  7. "The kink-turn: a new RNA secondary structure motif."
    Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
    EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  8. "The structures of four macrolide antibiotics bound to the large ribosomal subunit."
    Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
    Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  9. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  10. "Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
    Hansen J.L., Moore P.B., Steitz T.A.
    J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
    Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
  11. "Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
    Schmeing T.M., Moore P.B., Steitz T.A.
    RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
  12. "Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
    Gabdulkhakov A., Nikonov S., Garber M.
    Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.

Entry informationi

Entry nameiRL32_HALMA
AccessioniPrimary (citable) accession number: P12736
Secondary accession number(s): Q5V1T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi