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P12736

- RL32_HALMA

UniProt

P12736 - RL32_HALMA

Protein

50S ribosomal protein L32e

Gene

rpl32e

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Binds to the 23S rRNA.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. nucleotide binding Source: InterPro
    3. rRNA binding Source: UniProtKB-KW
    4. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. DNA repair Source: InterPro
    2. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciHMAR272569:GJDH-1451-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L32e
    Alternative name(s):
    Hl5
    Gene namesi
    Name:rpl32e
    Ordered Locus Names:rrnAC1595
    OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
    Taxonomic identifieri272569 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
    ProteomesiUP000001169: Chromosome I

    Subcellular locationi

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 24124050S ribosomal protein L32ePRO_0000131148Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit. Interacts weakly with protein L15.2 Publications

    Protein-protein interaction databases

    STRINGi272569.rrnAC1595.

    Structurei

    Secondary structure

    1
    241
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi97 – 1004
    Helixi112 – 12413
    Helixi134 – 1363
    Beta strandi138 – 1403
    Turni153 – 1575
    Helixi167 – 1693
    Turni173 – 1775
    Beta strandi184 – 1907
    Helixi191 – 1944
    Turni199 – 2013
    Beta strandi202 – 2065
    Beta strandi208 – 2103
    Helixi212 – 22413
    Beta strandi229 – 2313
    Beta strandi234 – 2374

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FFKX-ray2.40V99-241[»]
    1JJ2X-ray2.40X2-241[»]
    1K73X-ray3.01Z2-241[»]
    1K8AX-ray3.00Z2-241[»]
    1K9MX-ray3.00Z2-241[»]
    1KC8X-ray3.01Z2-241[»]
    1KD1X-ray3.00Z2-241[»]
    1KQSX-ray3.10X2-241[»]
    1M1KX-ray3.20Z2-241[»]
    1M90X-ray2.80Z2-241[»]
    1N8RX-ray3.00Z2-241[»]
    1NJIX-ray3.00Z2-241[»]
    1Q7YX-ray3.20Z2-241[»]
    1Q81X-ray2.95Z2-241[»]
    1Q82X-ray2.98Z2-241[»]
    1Q86X-ray3.00Z2-241[»]
    1QVFX-ray3.10X2-241[»]
    1QVGX-ray2.90X2-241[»]
    1S72X-ray2.40Y1-241[»]
    1VQ4X-ray2.70Y1-241[»]
    1VQ5X-ray2.60Y1-241[»]
    1VQ6X-ray2.70Y1-241[»]
    1VQ7X-ray2.50Y1-241[»]
    1VQ8X-ray2.20Y1-241[»]
    1VQ9X-ray2.40Y1-241[»]
    1VQKX-ray2.30Y1-241[»]
    1VQLX-ray2.30Y1-241[»]
    1VQMX-ray2.30Y1-241[»]
    1VQNX-ray2.40Y1-241[»]
    1VQOX-ray2.20Y1-241[»]
    1VQPX-ray2.25Y1-241[»]
    1W2BX-ray3.50X2-241[»]
    1YHQX-ray2.40Y1-241[»]
    1YI2X-ray2.65Y1-241[»]
    1YIJX-ray2.60Y1-241[»]
    1YITX-ray2.80Y1-241[»]
    1YJ9X-ray2.90Y1-241[»]
    1YJNX-ray3.00Y1-241[»]
    1YJWX-ray2.90Y1-241[»]
    2OTJX-ray2.90Y1-241[»]
    2OTLX-ray2.70Y1-241[»]
    2QA4X-ray3.00Y1-241[»]
    2QEXX-ray2.90Y1-241[»]
    3CC2X-ray2.40Y1-241[»]
    3CC4X-ray2.70Y1-241[»]
    3CC7X-ray2.70Y1-241[»]
    3CCEX-ray2.75Y1-241[»]
    3CCJX-ray2.70Y1-241[»]
    3CCLX-ray2.90Y1-241[»]
    3CCMX-ray2.55Y1-241[»]
    3CCQX-ray2.90Y1-241[»]
    3CCRX-ray3.00Y1-241[»]
    3CCSX-ray2.95Y1-241[»]
    3CCUX-ray2.80Y1-241[»]
    3CCVX-ray2.90Y1-241[»]
    3CD6X-ray2.75Y1-241[»]
    3CMAX-ray2.80Y1-240[»]
    3CMEX-ray2.95Y1-240[»]
    3CPWX-ray2.70X1-241[»]
    3CXCX-ray3.00X2-241[»]
    3G4SX-ray3.20Y96-237[»]
    3G6EX-ray2.70Y96-237[»]
    3G71X-ray2.85Y96-237[»]
    3I55X-ray3.11Y1-241[»]
    3I56X-ray2.90Y1-241[»]
    3OW2X-ray2.70X96-237[»]
    4ADXelectron microscopy6.60Y1-241[»]
    4HUBX-ray2.40Y1-241[»]
    ProteinModelPortaliP12736.
    SMRiP12736. Positions 96-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12736.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L32e family.Curated

    Phylogenomic databases

    eggNOGiCOG1717.
    HOGENOMiHOG000038489.
    KOiK02912.
    OMAiKPQFNRQ.

    Family and domain databases

    HAMAPiMF_00810. Ribosomal_L32e.
    InterProiIPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR001515. Ribosomal_L32e.
    IPR023654. Ribosomal_L32e_arc.
    IPR018263. Ribosomal_L32e_CS.
    [Graphical view]
    PfamiPF01655. Ribosomal_L32e. 1 hit.
    [Graphical view]
    ProDomiPD003823. Ribosomal_L32e. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00278. HhH1. 2 hits.
    [Graphical view]
    SUPFAMiSSF47794. SSF47794. 1 hit.
    SSF52042. SSF52042. 1 hit.
    PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12736-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADNEEDVEA EEEYTELTDI SGVGPSKAES LREAGFESVE DVRGADQSAL    50
    ADVSGIGNAL AARIKADVGG LEVESETEAE VEEEGGEEAP DEDVETELQA 100
    RGLTEKTPDL SDEDARLLTQ RHRVGKPQFN RQDHHKKKRV STSWRKPRGQ 150
    LSKQRRGIKG KGDTVEAGFR SPTAVRGKHP SGFEEVRVHN VDDLEGVDGD 200
    TEAVRIASKV GARKRERIEE EAEDAGIRVL NPTYVEVEVS E 241
    Length:241
    Mass (Da):26,416
    Last modified:January 23, 2007 - v3
    Checksum:iF4FFF58DE34B3711
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261S → R AA sequence (PubMed:3196689)Curated
    Sequence conflicti31 – 311Missing AA sequence (PubMed:3196689)Curated
    Sequence conflicti110 – 1112LS → SL AA sequence (PubMed:3196689)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58395 Genomic DNA. Translation: CAA41288.1.
    AY596297 Genomic DNA. Translation: AAV46513.1.
    PIRiS16539. R7HSH5.
    RefSeqiWP_011223739.1. NC_006396.1.
    YP_136219.1. NC_006396.1.

    Genome annotation databases

    EnsemblBacteriaiAAV46513; AAV46513; rrnAC1595.
    GeneIDi3128514.
    KEGGihma:rrnAC1595.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58395 Genomic DNA. Translation: CAA41288.1 .
    AY596297 Genomic DNA. Translation: AAV46513.1 .
    PIRi S16539. R7HSH5.
    RefSeqi WP_011223739.1. NC_006396.1.
    YP_136219.1. NC_006396.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FFK X-ray 2.40 V 99-241 [» ]
    1JJ2 X-ray 2.40 X 2-241 [» ]
    1K73 X-ray 3.01 Z 2-241 [» ]
    1K8A X-ray 3.00 Z 2-241 [» ]
    1K9M X-ray 3.00 Z 2-241 [» ]
    1KC8 X-ray 3.01 Z 2-241 [» ]
    1KD1 X-ray 3.00 Z 2-241 [» ]
    1KQS X-ray 3.10 X 2-241 [» ]
    1M1K X-ray 3.20 Z 2-241 [» ]
    1M90 X-ray 2.80 Z 2-241 [» ]
    1N8R X-ray 3.00 Z 2-241 [» ]
    1NJI X-ray 3.00 Z 2-241 [» ]
    1Q7Y X-ray 3.20 Z 2-241 [» ]
    1Q81 X-ray 2.95 Z 2-241 [» ]
    1Q82 X-ray 2.98 Z 2-241 [» ]
    1Q86 X-ray 3.00 Z 2-241 [» ]
    1QVF X-ray 3.10 X 2-241 [» ]
    1QVG X-ray 2.90 X 2-241 [» ]
    1S72 X-ray 2.40 Y 1-241 [» ]
    1VQ4 X-ray 2.70 Y 1-241 [» ]
    1VQ5 X-ray 2.60 Y 1-241 [» ]
    1VQ6 X-ray 2.70 Y 1-241 [» ]
    1VQ7 X-ray 2.50 Y 1-241 [» ]
    1VQ8 X-ray 2.20 Y 1-241 [» ]
    1VQ9 X-ray 2.40 Y 1-241 [» ]
    1VQK X-ray 2.30 Y 1-241 [» ]
    1VQL X-ray 2.30 Y 1-241 [» ]
    1VQM X-ray 2.30 Y 1-241 [» ]
    1VQN X-ray 2.40 Y 1-241 [» ]
    1VQO X-ray 2.20 Y 1-241 [» ]
    1VQP X-ray 2.25 Y 1-241 [» ]
    1W2B X-ray 3.50 X 2-241 [» ]
    1YHQ X-ray 2.40 Y 1-241 [» ]
    1YI2 X-ray 2.65 Y 1-241 [» ]
    1YIJ X-ray 2.60 Y 1-241 [» ]
    1YIT X-ray 2.80 Y 1-241 [» ]
    1YJ9 X-ray 2.90 Y 1-241 [» ]
    1YJN X-ray 3.00 Y 1-241 [» ]
    1YJW X-ray 2.90 Y 1-241 [» ]
    2OTJ X-ray 2.90 Y 1-241 [» ]
    2OTL X-ray 2.70 Y 1-241 [» ]
    2QA4 X-ray 3.00 Y 1-241 [» ]
    2QEX X-ray 2.90 Y 1-241 [» ]
    3CC2 X-ray 2.40 Y 1-241 [» ]
    3CC4 X-ray 2.70 Y 1-241 [» ]
    3CC7 X-ray 2.70 Y 1-241 [» ]
    3CCE X-ray 2.75 Y 1-241 [» ]
    3CCJ X-ray 2.70 Y 1-241 [» ]
    3CCL X-ray 2.90 Y 1-241 [» ]
    3CCM X-ray 2.55 Y 1-241 [» ]
    3CCQ X-ray 2.90 Y 1-241 [» ]
    3CCR X-ray 3.00 Y 1-241 [» ]
    3CCS X-ray 2.95 Y 1-241 [» ]
    3CCU X-ray 2.80 Y 1-241 [» ]
    3CCV X-ray 2.90 Y 1-241 [» ]
    3CD6 X-ray 2.75 Y 1-241 [» ]
    3CMA X-ray 2.80 Y 1-240 [» ]
    3CME X-ray 2.95 Y 1-240 [» ]
    3CPW X-ray 2.70 X 1-241 [» ]
    3CXC X-ray 3.00 X 2-241 [» ]
    3G4S X-ray 3.20 Y 96-237 [» ]
    3G6E X-ray 2.70 Y 96-237 [» ]
    3G71 X-ray 2.85 Y 96-237 [» ]
    3I55 X-ray 3.11 Y 1-241 [» ]
    3I56 X-ray 2.90 Y 1-241 [» ]
    3OW2 X-ray 2.70 X 96-237 [» ]
    4ADX electron microscopy 6.60 Y 1-241 [» ]
    4HUB X-ray 2.40 Y 1-241 [» ]
    ProteinModelPortali P12736.
    SMRi P12736. Positions 96-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272569.rrnAC1595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV46513 ; AAV46513 ; rrnAC1595 .
    GeneIDi 3128514.
    KEGGi hma:rrnAC1595.

    Phylogenomic databases

    eggNOGi COG1717.
    HOGENOMi HOG000038489.
    KOi K02912.
    OMAi KPQFNRQ.

    Enzyme and pathway databases

    BioCyci HMAR272569:GJDH-1451-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P12736.

    Family and domain databases

    HAMAPi MF_00810. Ribosomal_L32e.
    InterProi IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR001515. Ribosomal_L32e.
    IPR023654. Ribosomal_L32e_arc.
    IPR018263. Ribosomal_L32e_CS.
    [Graphical view ]
    Pfami PF01655. Ribosomal_L32e. 1 hit.
    [Graphical view ]
    ProDomi PD003823. Ribosomal_L32e. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00278. HhH1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47794. SSF47794. 1 hit.
    SSF52042. SSF52042. 1 hit.
    PROSITEi PS00580. RIBOSOMAL_L32E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization and nucleotide sequence of ten ribosomal protein genes from the region equivalent to the spectinomycin operon in the archaebacterium Halobacterium marismortui."
      Scholzen T., Arndt E.
      Mol. Gen. Genet. 228:70-80(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    3. "Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane."
      Walsh M.J., McDougall J., Wittmann-Liebold B.
      Biochemistry 27:6867-6876(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-32 AND 98-113.
    4. "The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
      Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
      Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 99-241.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    5. "The structural basis of ribosome activity in peptide bond synthesis."
      Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
      Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    6. "A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
      Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
      Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    7. "The kink-turn: a new RNA secondary structure motif."
      Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
      EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    8. "The structures of four macrolide antibiotics bound to the large ribosomal subunit."
      Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
      Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    9. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    10. "Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
      Hansen J.L., Moore P.B., Steitz T.A.
      J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    11. "Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
      Schmeing T.M., Moore P.B., Steitz T.A.
      RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
    12. "Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
      Gabdulkhakov A., Nikonov S., Garber M.
      Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.

    Entry informationi

    Entry nameiRL32_HALMA
    AccessioniPrimary (citable) accession number: P12736
    Secondary accession number(s): Q5V1T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3