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P12734

- RL21_HALMA

UniProt

P12734 - RL21_HALMA

Protein

50S ribosomal protein L21e

Gene

rpl21e

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This is one of 5 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit, stabilizing the orientation of adjacent RNA domains.

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciHMAR272569:GJDH-236-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L21e
    Alternative name(s):
    Hl31
    Gene namesi
    Name:rpl21e
    Ordered Locus Names:rrnAC0260
    OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
    Taxonomic identifieri272569 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
    ProteomesiUP000001169: Chromosome I

    Subcellular locationi

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 969550S ribosomal protein L21ePRO_0000149686Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit. Interacts with protein L18 and binds the 5S rRNA. Has been cross-linked to L18.2 Publications

    Protein-protein interaction databases

    STRINGi272569.rrnAC0260.

    Structurei

    Secondary structure

    1
    96
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni7 – 104
    Turni12 – 154
    Helixi19 – 213
    Helixi28 – 314
    Beta strandi39 – 424
    Helixi55 – 573
    Beta strandi61 – 688
    Beta strandi71 – 788
    Beta strandi81 – 877
    Helixi89 – 913
    Beta strandi92 – 943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FFKX-ray2.40N2-96[»]
    1JJ2X-ray2.40P2-96[»]
    1K73X-ray3.01R2-96[»]
    1K8AX-ray3.00R2-96[»]
    1K9MX-ray3.00R2-96[»]
    1KC8X-ray3.01R2-96[»]
    1KD1X-ray3.00R2-96[»]
    1KQSX-ray3.10P2-96[»]
    1M1KX-ray3.20R2-96[»]
    1M90X-ray2.80R2-96[»]
    1N8RX-ray3.00R2-96[»]
    1NJIX-ray3.00R2-96[»]
    1Q7YX-ray3.20R2-96[»]
    1Q81X-ray2.95R2-96[»]
    1Q82X-ray2.98R2-96[»]
    1Q86X-ray3.00R2-96[»]
    1QVFX-ray3.10P2-96[»]
    1QVGX-ray2.90P2-96[»]
    1S72X-ray2.40Q1-96[»]
    1VQ4X-ray2.70Q1-96[»]
    1VQ5X-ray2.60Q1-96[»]
    1VQ6X-ray2.70Q1-96[»]
    1VQ7X-ray2.50Q1-96[»]
    1VQ8X-ray2.20Q1-96[»]
    1VQ9X-ray2.40Q1-96[»]
    1VQKX-ray2.30Q1-96[»]
    1VQLX-ray2.30Q1-96[»]
    1VQMX-ray2.30Q1-96[»]
    1VQNX-ray2.40Q1-96[»]
    1VQOX-ray2.20Q1-96[»]
    1VQPX-ray2.25Q1-96[»]
    1W2BX-ray3.50P2-96[»]
    1YHQX-ray2.40Q1-96[»]
    1YI2X-ray2.65Q1-96[»]
    1YIJX-ray2.60Q1-96[»]
    1YITX-ray2.80Q1-96[»]
    1YJ9X-ray2.90Q1-96[»]
    1YJNX-ray3.00Q1-96[»]
    1YJWX-ray2.90Q1-96[»]
    2OTJX-ray2.90Q1-96[»]
    2OTLX-ray2.70Q1-96[»]
    2QA4X-ray3.00Q1-96[»]
    2QEXX-ray2.90Q1-96[»]
    3CC2X-ray2.40Q1-96[»]
    3CC4X-ray2.70Q1-96[»]
    3CC7X-ray2.70Q1-96[»]
    3CCEX-ray2.75Q1-96[»]
    3CCJX-ray2.70Q1-96[»]
    3CCLX-ray2.90Q1-96[»]
    3CCMX-ray2.55Q1-96[»]
    3CCQX-ray2.90Q1-96[»]
    3CCRX-ray3.00Q1-96[»]
    3CCSX-ray2.95Q1-96[»]
    3CCUX-ray2.80Q1-96[»]
    3CCVX-ray2.90Q1-96[»]
    3CD6X-ray2.75Q1-96[»]
    3CMAX-ray2.80Q1-96[»]
    3CMEX-ray2.95Q1-96[»]
    3CPWX-ray2.70P1-96[»]
    3CXCX-ray3.00P2-96[»]
    3G4SX-ray3.20Q2-96[»]
    3G6EX-ray2.70Q2-96[»]
    3G71X-ray2.85Q2-96[»]
    3I55X-ray3.11Q1-96[»]
    3I56X-ray2.90Q1-96[»]
    3OW2X-ray2.70P2-96[»]
    4ADXelectron microscopy6.60Q1-96[»]
    4HUBX-ray2.40Q1-96[»]
    ProteinModelPortaliP12734.
    SMRiP12734. Positions 2-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12734.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L21e family.Curated

    Phylogenomic databases

    eggNOGiCOG2139.
    HOGENOMiHOG000106438.
    KOiK02889.
    OMAiPRFHGRT.

    Family and domain databases

    Gene3Di2.30.30.70. 1 hit.
    HAMAPiMF_00369. Ribosomal_L21e.
    InterProiIPR001147. Ribosomal_L21e.
    IPR022856. Ribosomal_L21e_arc.
    IPR018259. Ribosomal_L21e_CS.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view]
    PfamiPF01157. Ribosomal_L21e. 1 hit.
    [Graphical view]
    SUPFAMiSSF50104. SSF50104. 1 hit.
    PROSITEiPS01171. RIBOSOMAL_L21E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12734-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSSNGPLEG TRGKLKNKPR DRGTSPPQRA VEEFDDGEKV HLKIDPSVPN   50
    GRFHPRFDGQ TGTVEGKQGD AYKVDIVDGG KEKTIIVTAA HLRRQE 96
    Length:96
    Mass (Da):10,548
    Last modified:January 23, 2007 - v2
    Checksum:iDE6DE036844EF1F2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY596297 Genomic DNA. Translation: AAV45317.1.
    PIRiS00370. R5HS31.
    RefSeqiYP_135023.1. NC_006396.1.

    Genome annotation databases

    EnsemblBacteriaiAAV45317; AAV45317; rrnAC0260.
    GeneIDi3129654.
    KEGGihma:rrnAC0260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY596297 Genomic DNA. Translation: AAV45317.1 .
    PIRi S00370. R5HS31.
    RefSeqi YP_135023.1. NC_006396.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FFK X-ray 2.40 N 2-96 [» ]
    1JJ2 X-ray 2.40 P 2-96 [» ]
    1K73 X-ray 3.01 R 2-96 [» ]
    1K8A X-ray 3.00 R 2-96 [» ]
    1K9M X-ray 3.00 R 2-96 [» ]
    1KC8 X-ray 3.01 R 2-96 [» ]
    1KD1 X-ray 3.00 R 2-96 [» ]
    1KQS X-ray 3.10 P 2-96 [» ]
    1M1K X-ray 3.20 R 2-96 [» ]
    1M90 X-ray 2.80 R 2-96 [» ]
    1N8R X-ray 3.00 R 2-96 [» ]
    1NJI X-ray 3.00 R 2-96 [» ]
    1Q7Y X-ray 3.20 R 2-96 [» ]
    1Q81 X-ray 2.95 R 2-96 [» ]
    1Q82 X-ray 2.98 R 2-96 [» ]
    1Q86 X-ray 3.00 R 2-96 [» ]
    1QVF X-ray 3.10 P 2-96 [» ]
    1QVG X-ray 2.90 P 2-96 [» ]
    1S72 X-ray 2.40 Q 1-96 [» ]
    1VQ4 X-ray 2.70 Q 1-96 [» ]
    1VQ5 X-ray 2.60 Q 1-96 [» ]
    1VQ6 X-ray 2.70 Q 1-96 [» ]
    1VQ7 X-ray 2.50 Q 1-96 [» ]
    1VQ8 X-ray 2.20 Q 1-96 [» ]
    1VQ9 X-ray 2.40 Q 1-96 [» ]
    1VQK X-ray 2.30 Q 1-96 [» ]
    1VQL X-ray 2.30 Q 1-96 [» ]
    1VQM X-ray 2.30 Q 1-96 [» ]
    1VQN X-ray 2.40 Q 1-96 [» ]
    1VQO X-ray 2.20 Q 1-96 [» ]
    1VQP X-ray 2.25 Q 1-96 [» ]
    1W2B X-ray 3.50 P 2-96 [» ]
    1YHQ X-ray 2.40 Q 1-96 [» ]
    1YI2 X-ray 2.65 Q 1-96 [» ]
    1YIJ X-ray 2.60 Q 1-96 [» ]
    1YIT X-ray 2.80 Q 1-96 [» ]
    1YJ9 X-ray 2.90 Q 1-96 [» ]
    1YJN X-ray 3.00 Q 1-96 [» ]
    1YJW X-ray 2.90 Q 1-96 [» ]
    2OTJ X-ray 2.90 Q 1-96 [» ]
    2OTL X-ray 2.70 Q 1-96 [» ]
    2QA4 X-ray 3.00 Q 1-96 [» ]
    2QEX X-ray 2.90 Q 1-96 [» ]
    3CC2 X-ray 2.40 Q 1-96 [» ]
    3CC4 X-ray 2.70 Q 1-96 [» ]
    3CC7 X-ray 2.70 Q 1-96 [» ]
    3CCE X-ray 2.75 Q 1-96 [» ]
    3CCJ X-ray 2.70 Q 1-96 [» ]
    3CCL X-ray 2.90 Q 1-96 [» ]
    3CCM X-ray 2.55 Q 1-96 [» ]
    3CCQ X-ray 2.90 Q 1-96 [» ]
    3CCR X-ray 3.00 Q 1-96 [» ]
    3CCS X-ray 2.95 Q 1-96 [» ]
    3CCU X-ray 2.80 Q 1-96 [» ]
    3CCV X-ray 2.90 Q 1-96 [» ]
    3CD6 X-ray 2.75 Q 1-96 [» ]
    3CMA X-ray 2.80 Q 1-96 [» ]
    3CME X-ray 2.95 Q 1-96 [» ]
    3CPW X-ray 2.70 P 1-96 [» ]
    3CXC X-ray 3.00 P 2-96 [» ]
    3G4S X-ray 3.20 Q 2-96 [» ]
    3G6E X-ray 2.70 Q 2-96 [» ]
    3G71 X-ray 2.85 Q 2-96 [» ]
    3I55 X-ray 3.11 Q 1-96 [» ]
    3I56 X-ray 2.90 Q 1-96 [» ]
    3OW2 X-ray 2.70 P 2-96 [» ]
    4ADX electron microscopy 6.60 Q 1-96 [» ]
    4HUB X-ray 2.40 Q 1-96 [» ]
    ProteinModelPortali P12734.
    SMRi P12734. Positions 2-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272569.rrnAC0260.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV45317 ; AAV45317 ; rrnAC0260 .
    GeneIDi 3129654.
    KEGGi hma:rrnAC0260.

    Phylogenomic databases

    eggNOGi COG2139.
    HOGENOMi HOG000106438.
    KOi K02889.
    OMAi PRFHGRT.

    Enzyme and pathway databases

    BioCyci HMAR272569:GJDH-236-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P12734.

    Family and domain databases

    Gene3Di 2.30.30.70. 1 hit.
    HAMAPi MF_00369. Ribosomal_L21e.
    InterProi IPR001147. Ribosomal_L21e.
    IPR022856. Ribosomal_L21e_arc.
    IPR018259. Ribosomal_L21e_CS.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view ]
    Pfami PF01157. Ribosomal_L21e. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50104. SSF50104. 1 hit.
    PROSITEi PS01171. RIBOSOMAL_L21E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    2. "Complete amino acid sequences of the ribosomal proteins L25, L29 and L31 from the archaebacterium Halobacterium marismortui."
      Hatakeyama T., Kimura M.
      Eur. J. Biochem. 172:703-711(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-96.
    3. "Localization of proteins HL29 and HL31 from Haloarcula marismortui within the 50 S ribosomal subunit by chemical crosslinking."
      Bergmann U., Wittmann-Liebold B.
      J. Mol. Biol. 232:693-700(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CROSS-LINKING TO L18.
    4. "The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
      Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
      Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    5. "The structural basis of ribosome activity in peptide bond synthesis."
      Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
      Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    6. "A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
      Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
      Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    7. "The kink-turn: a new RNA secondary structure motif."
      Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
      EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    8. "The structures of four macrolide antibiotics bound to the large ribosomal subunit."
      Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
      Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    9. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    10. "Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
      Hansen J.L., Moore P.B., Steitz T.A.
      J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    11. "Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
      Schmeing T.M., Moore P.B., Steitz T.A.
      RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
    12. "Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
      Gabdulkhakov A., Nikonov S., Garber M.
      Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.

    Entry informationi

    Entry nameiRL21_HALMA
    AccessioniPrimary (citable) accession number: P12734
    Secondary accession number(s): Q5V585
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3