Reviewed, UniProtKB/Swiss-Prot P12732 (RL23_HALMA)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L23P
Alternative name(s):
    Hmal23
    Hl25
    L21

Gene names

Name:	rpl23p
Ordered Locus Names:	rrnAC1609

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to a specific region on the 23S rRNA. Located at the polypeptide exit tunnel on the outside of the subunit. HAMAP MF_01369
Subunit structure	Part of the 50S ribosomal subunit. Interacts with protein L29 and weakly with protein L39e. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the ribosomal protein L23P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.4

Chain

2 – 85

84

50S ribosomal protein L23P HAMAP MF_01369

PRO_0000129435

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

85

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Helix

13 – 21

9

Beta strand

24 – 29

6

Helix

35 – 46

12

Beta strand

50 – 57

8

Beta strand

61 – 69

9

Helix

75 – 79

5

Sequences

	Sequence		Length	Mass (Da)
	P12732-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 2A2E869D640D5B60	FASTA	85	9,602
	10 20 30 40 50 60 MSWDVIKHPH VTEKAMNDMD FQNKLQFAVD DRASKGEVAD AVEEQYDVTV EQVNTQNTMD 70 80 GEKKAVVRLS EDDDAQEVAS RIGVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete amino acid sequences of the ribosomal proteins L25, L29 and L31 from the archaebacterium Halobacterium marismortui." Hatakeyama T., Kimura M. Eur. J. Biochem. 172:703-711(1988) [PubMed: 3350019] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-85.
[2]	"Organization and nucleotide sequence of a gene cluster coding for eight ribosomal proteins in the archaebacterium Halobacterium marismortui." Arndt E., Kroemer W., Hatakeyama T. J. Biol. Chem. 265:3034-3039(1990) [PubMed: 2406244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[4]	"Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane." Walsh M.J., McDougall J., Wittmann-Liebold B. Biochemistry 27:6867-6876(1988) [PubMed: 3196689] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-23.
[5]	"The complete atomic structure of the large ribosomal subunit at 2.4 A resolution." Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A. Science 289:905-920(2000) [PubMed: 10937989] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[6]	"The structural basis of ribosome activity in peptide bond synthesis." Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A. Science 289:920-930(2000) [PubMed: 10937990] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[7]	"A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits." Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A. Nat. Struct. Biol. 9:225-230(2002) [PubMed: 11828326] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[8]	"The kink-turn: a new RNA secondary structure motif." Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A. EMBO J. 20:4214-4221(2001) [PubMed: 11483524] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[9]	"The structures of four macrolide antibiotics bound to the large ribosomal subunit." Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A. Mol. Cell 10:117-128(2002) [PubMed: 12150912] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[10]	"Structural insights into peptide bond formation." Hansen J.L., Schmeing T.M., Moore P.B., Steitz T.A. Proc. Natl. Acad. Sci. U.S.A. 99:11670-11675(2002) [PubMed: 12185246] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[11]	"Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit." Hansen J.L., Moore P.B., Steitz T.A. J. Mol. Biol. 330:1061-1075(2003) [PubMed: 12860128] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[12]	"Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit." Schmeing T.M., Moore P.B., Steitz T.A. RNA 9:1345-1352(2003) [PubMed: 14561884] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J05222 Genomic DNA. Translation: AAA86861.1.
AY596297 Genomic DNA. Translation: AAV46526.1.

PIR

R5HS23. E35063.

RefSeq

YP_136232.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FFK	X-ray	2.40	P	2-85	[»]
1JJ2	X-ray	2.40	R	2-85	[»]
1K73	X-ray	3.01	T	2-85	[»]
1K8A	X-ray	3.00	T	2-85	[»]
1K9M	X-ray	3.00	T	2-85	[»]
1KC8	X-ray	3.01	T	2-85	[»]
1KD1	X-ray	3.00	T	2-85	[»]
1KQS	X-ray	3.10	R	2-85	[»]
1M1K	X-ray	3.20	T	2-85	[»]
1M90	X-ray	2.80	T	2-85	[»]
1N8R	X-ray	3.00	T	2-85	[»]
1NJI	X-ray	3.00	T	2-85	[»]
1Q7Y	X-ray	3.20	T	2-85	[»]
1Q81	X-ray	2.95	T	2-85	[»]
1Q82	X-ray	2.98	T	2-85	[»]
1Q86	X-ray	3.00	T	2-85	[»]
1QVF	X-ray	3.10	R	2-85	[»]
1QVG	X-ray	2.90	R	2-85	[»]
1S72	X-ray	2.40	S	1-85	[»]
1VQ4	X-ray	2.70	S	1-85	[»]
1VQ5	X-ray	2.60	S	1-85	[»]
1VQ6	X-ray	2.70	S	1-85	[»]
1VQ7	X-ray	2.50	S	1-85	[»]
1VQ8	X-ray	2.20	S	1-85	[»]
1VQ9	X-ray	2.40	S	1-85	[»]
1VQK	X-ray	2.30	S	1-85	[»]
1VQL	X-ray	2.30	S	1-85	[»]
1VQM	X-ray	2.30	S	1-85	[»]
1VQN	X-ray	2.40	S	1-85	[»]
1VQO	X-ray	2.20	S	1-85	[»]
1VQP	X-ray	2.25	S	1-85	[»]
1W2B	X-ray	3.50	R	2-84	[»]
1YHQ	X-ray	2.40	S	1-85	[»]
1YI2	X-ray	2.65	S	1-85	[»]
1YIJ	X-ray	2.60	S	1-85	[»]
1YIT	X-ray	2.80	S	1-85	[»]
1YJ9	X-ray	2.90	S	1-85	[»]
1YJN	X-ray	3.00	S	1-85	[»]
1YJW	X-ray	2.90	S	1-85	[»]
1YL3	X-ray	5.50	T	2-85	[»]
2OTJ	X-ray	2.90	S	1-85	[»]
2OTL	X-ray	2.70	S	1-85	[»]
2QA4	X-ray	3.00	S	1-85	[»]
2QEX	X-ray	2.90	S	1-85	[»]
3CC2	X-ray	2.40	S	1-85	[»]
3CC4	X-ray	2.70	S	1-85	[»]
3CC7	X-ray	2.70	S	1-85	[»]
3CCE	X-ray	2.75	S	1-85	[»]
3CCJ	X-ray	2.70	S	1-85	[»]
3CCL	X-ray	2.90	S	1-85	[»]
3CCM	X-ray	2.55	S	1-85	[»]
3CCQ	X-ray	2.90	S	1-85	[»]
3CCR	X-ray	3.00	S	1-85	[»]
3CCS	X-ray	2.95	S	1-85	[»]
3CCU	X-ray	2.80	S	1-85	[»]
3CCV	X-ray	2.90	S	1-85	[»]
3CD6	X-ray	2.75	S	1-85	[»]
3CMA	X-ray	2.80	S	1-85	[»]
3CME	X-ray	2.95	S	1-85	[»]
3CPW	X-ray	2.70	R	1-85	[»]
3CXC	X-ray	3.00	R	2-84	[»]
3G4S	X-ray	3.20	S	2-82	[»]
3G6E	X-ray	2.70	S	2-82	[»]
3G71	X-ray	2.85	S	2-82	[»]

ModBase

Search...

Genome annotation databases

GeneID

3128415.

GenomeReviews

Gene locus rrnAC1609 in contig AY596297_GR.

KEGG

hma:rrnAC1609.

NMPDR

fig|272569.1.peg.1500.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P12732.

OMA

DENKLQF.

Enzyme and pathway databases

BioCyc

HMAR272569:RRNAC1609-MON.

Family and domain databases

HAMAP

MF_01369.
[Tree]

InterPro

IPR012677. a_b_plait_nuc_bd.
IPR019985. Ribosomal_L23.
IPR001014. Ribosomal_L23/L25_CS.
IPR013025. Ribosomal_L25/23.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.

Pfam

PF00276. Ribosomal_L23. 1 hit.
[Graphical view]

ProDom

PD001141. Ribosomal_L23. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR03636. L23_arch. 1 hit.

PROSITE

PS00050. RIBOSOMAL_L23. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL23_HALMA

Accession

Primary (citable) accession number: P12732
Secondary accession number(s): Q5V1S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)