Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eosinophil cationic protein

Gene

RNASE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei42Proton acceptor1
Sitei60May be involved in LPS-binding1
Sitei62May be involved in LPS- and LTA-binding1
Active sitei155Proton donor1

GO - Molecular functioni

  • endonuclease activity Source: GO_Central
  • nucleic acid binding Source: InterPro
  • ribonuclease activity Source: ProtInc

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • innate immune response in mucosa Source: UniProtKB
  • RNA catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciZFISH:HS09939-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.
R-HSA-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Eosinophil cationic protein (EC:3.1.27.-)
Short name:
ECP
Alternative name(s):
Ribonuclease 3
Short name:
RNase 3
Gene namesi
Name:RNASE3
Synonyms:ECP, RNS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:10046. RNASE3.

Subcellular locationi

  • Secreted

  • Note: Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular region Source: ProtInc
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40I → A: Loss of in vitro formation of amyloid-like aggregates. 1 Publication1

Organism-specific databases

DisGeNETi6037.
OpenTargetsiENSG00000169397.
PharmGKBiPA34414.

Chemistry databases

DrugBankiDB01411. Pranlukast.

Polymorphism and mutation databases

BioMutaiRNASE3.
DMDMi147744558.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 272 PublicationsAdd BLAST27
ChainiPRO_000003086228 – 160Eosinophil cationic proteinAdd BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 110
Modified residuei60Nitrated tyrosine1 Publication1
Disulfide bondi64 ↔ 123
Disulfide bondi82 ↔ 138
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi89 ↔ 98
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration

Proteomic databases

EPDiP12724.
PaxDbiP12724.
PeptideAtlasiP12724.
PRIDEiP12724.

PTM databases

iPTMnetiP12724.

Expressioni

Gene expression databases

BgeeiENSG00000169397.
CleanExiHS_RNASE3.
GenevisibleiP12724. HS.

Organism-specific databases

HPAiHPA056183.

Interactioni

Subunit structurei

Interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). In vitro interacts with and insert into lipid bilayers composed of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol. In vitro, tends to form amyloid-like aggregates at pH 3, but not at pH 5, nor 7.1 Publication

Protein-protein interaction databases

BioGridi111966. 13 interactors.
STRINGi9606.ENSP00000302324.

Structurei

Secondary structure

1160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 32Combined sources3
Helixi34 – 42Combined sources9
Helixi50 – 53Combined sources4
Helixi55 – 58Combined sources4
Beta strandi61 – 63Combined sources3
Beta strandi66 – 73Combined sources8
Helixi75 – 81Combined sources7
Beta strandi90 – 92Combined sources3
Turni93 – 95Combined sources3
Beta strandi98 – 100Combined sources3
Beta strandi105 – 113Combined sources9
Helixi120 – 122Combined sources3
Beta strandi125 – 132Combined sources8
Beta strandi134 – 140Combined sources7
Turni143 – 145Combined sources3
Beta strandi150 – 159Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DYTX-ray1.75A/B28-160[»]
1H1HX-ray2.00A28-160[»]
1QMTX-ray2.40A28-160[»]
2KB5NMR-A28-160[»]
2LVZNMR-A28-160[»]
4A2OX-ray1.69A/B28-160[»]
4A2YX-ray1.70A/B28-160[»]
4OWZX-ray1.47A/B28-160[»]
4OXBX-ray1.50A/B28-160[»]
4OXFX-ray1.50A/B28-160[»]
4X08X-ray1.34A/B28-160[»]
ProteinModelPortaliP12724.
SMRiP12724.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12724.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 72Required for nearly all of the bactericidal activity; partially involved in LPS-binding and bacterial membrane depolarizationAdd BLAST45
Regioni65 – 69Substrate binding5

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiP12724.
KOiK10787.
OMAiNQSIRCP.
OrthoDBiEOG091G0SFK.
PhylomeDBiP12724.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPKLFTSQI CLLLLLGLMG VEGSLHARPP QFTRAQWFAI QHISLNPPRC
60 70 80 90 100
TIAMRAINNY RWRCKNQNTF LRTTFANVVN VCGNQSIRCP HNRTLNNCHR
110 120 130 140 150
SRFRVPLLHC DLINPGAQNI SNCTYADRPG RRFYVVACDN RDPRDSPRYP
160
VVPVHLDTTI
Length:160
Mass (Da):18,385
Last modified:May 15, 2007 - v2
Checksum:iD7BED24F67B23FA9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01410972R → C.1 PublicationCorresponds to variant rs151169198dbSNPEnsembl.1
Natural variantiVAR_013149124T → R.7 PublicationsCorresponds to variant rs2073342dbSNPEnsembl.1
Natural variantiVAR_029017130G → R.Corresponds to variant rs12147890dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15161 mRNA. Translation: CAA33251.1.
M28128 mRNA. Translation: AAA50283.1.
X16545 Genomic DNA. Translation: CAA34545.1.
X55990 Genomic DNA. Translation: CAA39462.1.
AF294019 Genomic DNA. Translation: AAG31589.1.
AF294020 Genomic DNA. Translation: AAG31590.1.
AF294021 Genomic DNA. Translation: AAG31591.1.
AF294022 Genomic DNA. Translation: AAG31592.1.
AF294023 Genomic DNA. Translation: AAG31593.1.
AF294024 Genomic DNA. Translation: AAG31594.1.
AF294025 Genomic DNA. Translation: AAG31595.1.
AF294026 Genomic DNA. Translation: AAG31596.1.
AL133371 Genomic DNA. No translation available.
BC096060 mRNA. Translation: AAH96060.1.
BC096061 mRNA. Translation: AAH96061.1.
BC096062 mRNA. Translation: AAH96062.1.
AF441204 Genomic DNA. Translation: AAL35279.1.
AF441205 Genomic DNA. Translation: AAL35280.1.
AF441206 Genomic DNA. Translation: AAL35281.1.
CCDSiCCDS9560.1.
PIRiB35328. JL0106.
RefSeqiNP_002926.2. NM_002935.2.
UniGeneiHs.73839.

Genome annotation databases

EnsembliENST00000304639; ENSP00000302324; ENSG00000169397.
GeneIDi6037.
KEGGihsa:6037.
UCSCiuc001vyj.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15161 mRNA. Translation: CAA33251.1.
M28128 mRNA. Translation: AAA50283.1.
X16545 Genomic DNA. Translation: CAA34545.1.
X55990 Genomic DNA. Translation: CAA39462.1.
AF294019 Genomic DNA. Translation: AAG31589.1.
AF294020 Genomic DNA. Translation: AAG31590.1.
AF294021 Genomic DNA. Translation: AAG31591.1.
AF294022 Genomic DNA. Translation: AAG31592.1.
AF294023 Genomic DNA. Translation: AAG31593.1.
AF294024 Genomic DNA. Translation: AAG31594.1.
AF294025 Genomic DNA. Translation: AAG31595.1.
AF294026 Genomic DNA. Translation: AAG31596.1.
AL133371 Genomic DNA. No translation available.
BC096060 mRNA. Translation: AAH96060.1.
BC096061 mRNA. Translation: AAH96061.1.
BC096062 mRNA. Translation: AAH96062.1.
AF441204 Genomic DNA. Translation: AAL35279.1.
AF441205 Genomic DNA. Translation: AAL35280.1.
AF441206 Genomic DNA. Translation: AAL35281.1.
CCDSiCCDS9560.1.
PIRiB35328. JL0106.
RefSeqiNP_002926.2. NM_002935.2.
UniGeneiHs.73839.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DYTX-ray1.75A/B28-160[»]
1H1HX-ray2.00A28-160[»]
1QMTX-ray2.40A28-160[»]
2KB5NMR-A28-160[»]
2LVZNMR-A28-160[»]
4A2OX-ray1.69A/B28-160[»]
4A2YX-ray1.70A/B28-160[»]
4OWZX-ray1.47A/B28-160[»]
4OXBX-ray1.50A/B28-160[»]
4OXFX-ray1.50A/B28-160[»]
4X08X-ray1.34A/B28-160[»]
ProteinModelPortaliP12724.
SMRiP12724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111966. 13 interactors.
STRINGi9606.ENSP00000302324.

Chemistry databases

DrugBankiDB01411. Pranlukast.

PTM databases

iPTMnetiP12724.

Polymorphism and mutation databases

BioMutaiRNASE3.
DMDMi147744558.

Proteomic databases

EPDiP12724.
PaxDbiP12724.
PeptideAtlasiP12724.
PRIDEiP12724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304639; ENSP00000302324; ENSG00000169397.
GeneIDi6037.
KEGGihsa:6037.
UCSCiuc001vyj.4. human.

Organism-specific databases

CTDi6037.
DisGeNETi6037.
GeneCardsiRNASE3.
H-InvDBHIX0037784.
HGNCiHGNC:10046. RNASE3.
HPAiHPA056183.
MIMi131398. gene.
neXtProtiNX_P12724.
OpenTargetsiENSG00000169397.
PharmGKBiPA34414.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiP12724.
KOiK10787.
OMAiNQSIRCP.
OrthoDBiEOG091G0SFK.
PhylomeDBiP12724.
TreeFamiTF333393.

Enzyme and pathway databases

BioCyciZFISH:HS09939-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.
R-HSA-6803157. Antimicrobial peptides.

Miscellaneous databases

EvolutionaryTraceiP12724.
GeneWikiiEosinophil_cationic_protein.
GenomeRNAii6037.
PROiP12724.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169397.
CleanExiHS_RNASE3.
GenevisibleiP12724. HS.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECP_HUMAN
AccessioniPrimary (citable) accession number: P12724
Secondary accession number(s): Q4VBC1
, Q8WTP7, Q8WZ62, Q9GZN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 15, 2007
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.