SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P12709

- G6PI_YEAST

UniProt

P12709 - G6PI_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glucose-6-phosphate isomerase

Gene
PGI1, YBR196C, YBR1406
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei367 – 3671Proton donor By similarity
Active sitei398 – 3981 By similarity
Active sitei520 – 5201 By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: SGD

GO - Biological processi

  1. gluconeogenesis Source: SGD
  2. glycolytic process Source: SGD
  3. pentose-phosphate shunt Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6121.
YEAST:YBR196C-MONOMER.
SABIO-RKP12709.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGI1
Ordered Locus Names:YBR196C
ORF Names:YBR1406
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

SGDiS000000400. PGI1.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 554553Glucose-6-phosphate isomeraseUniRule annotationPRO_0000180578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei53 – 531Phosphothreonine1 Publication
Modified residuei220 – 2201Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP12709.
PaxDbiP12709.
PeptideAtlasiP12709.

Expressioni

Gene expression databases

GenevestigatoriP12709.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32893. 58 interactions.
DIPiDIP-1605N.
IntActiP12709. 18 interactions.
MINTiMINT-386473.
STRINGi4932.YBR196C.

Structurei

3D structure databases

ProteinModelPortaliP12709.
SMRiP12709. Positions 13-553.

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.

Phylogenomic databases

eggNOGiCOG0166.
GeneTreeiENSGT00390000000707.
HOGENOMiHOG000261371.
KOiK01810.
OMAiCGNELDN.
OrthoDBiEOG7N0CDV.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12709-1 [UniParc]FASTAAdd to Basket

« Hide

MSNNSFTNFK LATELPAWSK LQKIYESQGK TLSVKQEFQK DAKRFEKLNK    50
TFTNYDGSKI LFDYSKNLVN DEIIAALIEL AKEANVTGLR DAMFKGEHIN 100
STEDRAVYHV ALRNRANKPM YVDGVNVAPE VDSVLKHMKE FSEQVRSGEW 150
KGYTGKKITD VVNIGIGGSD LGPVMVTEAL KHYAGVLDVH FVSNIDGTHI 200
AETLKVVDPE TTLFLIASKT FTTAETITNA NTAKNWFLSK TGNDPSHIAK 250
HFAALSTNET EVAKFGIDTK NMFGFESWVG GRYSVWSAIG LSVALYIGYD 300
NFEAFLKGAE AVDNHFTQTP LEDNIPLLGG LLSVWYNNFF GAQTHLVAPF 350
DQYLHRFPAY LQQLSMESNG KSVTRGNVFT DYSTGSILFG EPATNAQHSF 400
FQLVHQGTKL IPSDFILAAQ SHNPIENKLH QKMLASNFFA QAEALMVGKD 450
EEQVKAEGAT GGLVPHKVFS GNRPTTSILA QKITPATLGA LIAYYEHVTF 500
TEGAIWNINS FDQWGVELGK VLAKVIGKEL DNSSTISTHD ASTNGLINQF 550
KEWM 554
Length:554
Mass (Da):61,299
Last modified:January 23, 2007 - v3
Checksum:i556D3D3B77EED052
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M37267 Genomic DNA. Translation: AAA34862.1.
M21696 Genomic DNA. Translation: AAA34894.1.
X13977 Genomic DNA. Translation: CAA32158.1.
Z21487 Genomic DNA. Translation: CAA79683.1.
Z36065 Genomic DNA. Translation: CAA85158.1.
BK006936 Genomic DNA. Translation: DAA07310.1.
PIRiJT0484. NUBY.
RefSeqiNP_009755.1. NM_001178544.1.

Genome annotation databases

EnsemblFungiiYBR196C; YBR196C; YBR196C.
GeneIDi852495.
KEGGisce:YBR196C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M37267 Genomic DNA. Translation: AAA34862.1 .
M21696 Genomic DNA. Translation: AAA34894.1 .
X13977 Genomic DNA. Translation: CAA32158.1 .
Z21487 Genomic DNA. Translation: CAA79683.1 .
Z36065 Genomic DNA. Translation: CAA85158.1 .
BK006936 Genomic DNA. Translation: DAA07310.1 .
PIRi JT0484. NUBY.
RefSeqi NP_009755.1. NM_001178544.1.

3D structure databases

ProteinModelPortali P12709.
SMRi P12709. Positions 13-553.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32893. 58 interactions.
DIPi DIP-1605N.
IntActi P12709. 18 interactions.
MINTi MINT-386473.
STRINGi 4932.YBR196C.

Chemistry

ChEMBLi CHEMBL1075250.

Proteomic databases

MaxQBi P12709.
PaxDbi P12709.
PeptideAtlasi P12709.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR196C ; YBR196C ; YBR196C .
GeneIDi 852495.
KEGGi sce:YBR196C.

Organism-specific databases

SGDi S000000400. PGI1.

Phylogenomic databases

eggNOGi COG0166.
GeneTreei ENSGT00390000000707.
HOGENOMi HOG000261371.
KOi K01810.
OMAi CGNELDN.
OrthoDBi EOG7N0CDV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00181 .
BioCyci MetaCyc:MONOMER-6121.
YEAST:YBR196C-MONOMER.
SABIO-RK P12709.

Miscellaneous databases

NextBioi 971489.
PROi P12709.

Gene expression databases

Genevestigatori P12709.

Family and domain databases

Gene3Di 1.10.1390.10. 1 hit.
HAMAPi MF_00473. G6P_isomerase.
InterProi IPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view ]
PANTHERi PTHR11469. PTHR11469. 1 hit.
Pfami PF00342. PGI. 1 hit.
[Graphical view ]
PRINTSi PR00662. G6PISOMERASE.
PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation, characterization and nucleotide sequence of the phosphoglucoisomerase gene of Saccharomyces cerevisiae."
    Tekamp-Olson P., Najarian R., Burke R.L.
    Gene 73:153-161(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae."
    Green J.B.A., Wright A.P.H., Cheung W.Y., Lancashire W.E., Hartley B.S.
    Mol. Gen. Genet. 215:100-106(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  3. "RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif."
    Demolis N., Mallet L., Bussereau F., Jacquet M.
    Yeast 9:645-659(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 10:819-831(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 424-554.
    Strain: ATCC 204508 / S288c.
  7. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
    Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
    Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiG6PI_YEAST
AccessioniPrimary (citable) accession number: P12709
Secondary accession number(s): D6VQJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 91600 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi