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P12709

- G6PI_YEAST

UniProt

P12709 - G6PI_YEAST

Protein

Glucose-6-phosphate isomerase

Gene

PGI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei367 – 3671Proton donorBy similarity
    Active sitei398 – 3981By similarity
    Active sitei520 – 5201By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate isomerase activity Source: SGD

    GO - Biological processi

    1. gluconeogenesis Source: SGD
    2. glycolytic process Source: SGD
    3. pentose-phosphate shunt Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Gluconeogenesis, Glycolysis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-6121.
    YEAST:YBR196C-MONOMER.
    SABIO-RKP12709.
    UniPathwayiUPA00109; UER00181.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate isomerase (EC:5.3.1.9)
    Short name:
    GPI
    Alternative name(s):
    Phosphoglucose isomerase
    Short name:
    PGI
    Phosphohexose isomerase
    Short name:
    PHI
    Gene namesi
    Name:PGI1
    Ordered Locus Names:YBR196C
    ORF Names:YBR1406
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    SGDiS000000400. PGI1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 554553Glucose-6-phosphate isomerasePRO_0000180578Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei53 – 531Phosphothreonine1 Publication
    Modified residuei220 – 2201Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP12709.
    PaxDbiP12709.
    PeptideAtlasiP12709.

    Expressioni

    Gene expression databases

    GenevestigatoriP12709.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi32893. 58 interactions.
    DIPiDIP-1605N.
    IntActiP12709. 18 interactions.
    MINTiMINT-386473.
    STRINGi4932.YBR196C.

    Structurei

    3D structure databases

    ProteinModelPortaliP12709.
    SMRiP12709. Positions 13-553.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI family.Curated

    Phylogenomic databases

    eggNOGiCOG0166.
    GeneTreeiENSGT00390000000707.
    HOGENOMiHOG000261371.
    KOiK01810.
    OMAiCGNELDN.
    OrthoDBiEOG7N0CDV.

    Family and domain databases

    Gene3Di1.10.1390.10. 1 hit.
    HAMAPiMF_00473. G6P_isomerase.
    InterProiIPR001672. G6P_Isomerase.
    IPR023096. G6P_Isomerase_C.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view]
    PANTHERiPTHR11469. PTHR11469. 1 hit.
    PfamiPF00342. PGI. 1 hit.
    [Graphical view]
    PRINTSiPR00662. G6PISOMERASE.
    PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNNSFTNFK LATELPAWSK LQKIYESQGK TLSVKQEFQK DAKRFEKLNK    50
    TFTNYDGSKI LFDYSKNLVN DEIIAALIEL AKEANVTGLR DAMFKGEHIN 100
    STEDRAVYHV ALRNRANKPM YVDGVNVAPE VDSVLKHMKE FSEQVRSGEW 150
    KGYTGKKITD VVNIGIGGSD LGPVMVTEAL KHYAGVLDVH FVSNIDGTHI 200
    AETLKVVDPE TTLFLIASKT FTTAETITNA NTAKNWFLSK TGNDPSHIAK 250
    HFAALSTNET EVAKFGIDTK NMFGFESWVG GRYSVWSAIG LSVALYIGYD 300
    NFEAFLKGAE AVDNHFTQTP LEDNIPLLGG LLSVWYNNFF GAQTHLVAPF 350
    DQYLHRFPAY LQQLSMESNG KSVTRGNVFT DYSTGSILFG EPATNAQHSF 400
    FQLVHQGTKL IPSDFILAAQ SHNPIENKLH QKMLASNFFA QAEALMVGKD 450
    EEQVKAEGAT GGLVPHKVFS GNRPTTSILA QKITPATLGA LIAYYEHVTF 500
    TEGAIWNINS FDQWGVELGK VLAKVIGKEL DNSSTISTHD ASTNGLINQF 550
    KEWM 554
    Length:554
    Mass (Da):61,299
    Last modified:January 23, 2007 - v3
    Checksum:i556D3D3B77EED052
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37267 Genomic DNA. Translation: AAA34862.1.
    M21696 Genomic DNA. Translation: AAA34894.1.
    X13977 Genomic DNA. Translation: CAA32158.1.
    Z21487 Genomic DNA. Translation: CAA79683.1.
    Z36065 Genomic DNA. Translation: CAA85158.1.
    BK006936 Genomic DNA. Translation: DAA07310.1.
    PIRiJT0484. NUBY.
    RefSeqiNP_009755.1. NM_001178544.1.

    Genome annotation databases

    EnsemblFungiiYBR196C; YBR196C; YBR196C.
    GeneIDi852495.
    KEGGisce:YBR196C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37267 Genomic DNA. Translation: AAA34862.1 .
    M21696 Genomic DNA. Translation: AAA34894.1 .
    X13977 Genomic DNA. Translation: CAA32158.1 .
    Z21487 Genomic DNA. Translation: CAA79683.1 .
    Z36065 Genomic DNA. Translation: CAA85158.1 .
    BK006936 Genomic DNA. Translation: DAA07310.1 .
    PIRi JT0484. NUBY.
    RefSeqi NP_009755.1. NM_001178544.1.

    3D structure databases

    ProteinModelPortali P12709.
    SMRi P12709. Positions 13-553.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32893. 58 interactions.
    DIPi DIP-1605N.
    IntActi P12709. 18 interactions.
    MINTi MINT-386473.
    STRINGi 4932.YBR196C.

    Chemistry

    ChEMBLi CHEMBL1075250.

    Proteomic databases

    MaxQBi P12709.
    PaxDbi P12709.
    PeptideAtlasi P12709.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR196C ; YBR196C ; YBR196C .
    GeneIDi 852495.
    KEGGi sce:YBR196C.

    Organism-specific databases

    SGDi S000000400. PGI1.

    Phylogenomic databases

    eggNOGi COG0166.
    GeneTreei ENSGT00390000000707.
    HOGENOMi HOG000261371.
    KOi K01810.
    OMAi CGNELDN.
    OrthoDBi EOG7N0CDV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00181 .
    BioCyci MetaCyc:MONOMER-6121.
    YEAST:YBR196C-MONOMER.
    SABIO-RK P12709.

    Miscellaneous databases

    NextBioi 971489.
    PROi P12709.

    Gene expression databases

    Genevestigatori P12709.

    Family and domain databases

    Gene3Di 1.10.1390.10. 1 hit.
    HAMAPi MF_00473. G6P_isomerase.
    InterProi IPR001672. G6P_Isomerase.
    IPR023096. G6P_Isomerase_C.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view ]
    PANTHERi PTHR11469. PTHR11469. 1 hit.
    Pfami PF00342. PGI. 1 hit.
    [Graphical view ]
    PRINTSi PR00662. G6PISOMERASE.
    PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The isolation, characterization and nucleotide sequence of the phosphoglucoisomerase gene of Saccharomyces cerevisiae."
      Tekamp-Olson P., Najarian R., Burke R.L.
      Gene 73:153-161(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae."
      Green J.B.A., Wright A.P.H., Cheung W.Y., Lancashire W.E., Hartley B.S.
      Mol. Gen. Genet. 215:100-106(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204510 / AB320.
    3. "RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif."
      Demolis N., Mallet L., Bussereau F., Jacquet M.
      Yeast 9:645-659(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family."
      Mallet L., Bussereau F., Jacquet M.
      Yeast 10:819-831(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 424-554.
      Strain: ATCC 204508 / S288c.
    7. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
      Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
      Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT SER-2.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiG6PI_YEAST
    AccessioniPrimary (citable) accession number: P12709
    Secondary accession number(s): D6VQJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 139 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 91600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3