Glucose-6-phosphate isomerase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P12709 (G6PI_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate isomerase
Short name=GPI
EC=5.3.1.9

Alternative name(s):
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI

Gene names

Name:	PGI1
Ordered Locus Names:	YBR196C
ORF Names:	YBR1406

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 91600 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the GPI family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from mutant phenotype. Source: SGD glycolysis Inferred from mutant phenotype. Source: SGD pentose-phosphate shunt Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	glucose-6-phosphate isomerase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 554

553

Glucose-6-phosphate isomerase

PRO_0000180578

Sites

Active site

367

Proton donor By similarity

Active site

398

By similarity

Active site

520

By similarity

Amino acid modifications

Modified residue

N-acetylserine Ref.7

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphothreonine Ref.12

Modified residue

Phosphothreonine Ref.12

Modified residue

102

Phosphothreonine Ref.12

Modified residue

193

Phosphoserine Ref.10

Modified residue

198

Phosphothreonine Ref.10

Modified residue

223

Phosphothreonine Ref.12

Modified residue

470

Phosphoserine Ref.12

Modified residue

534

Phosphoserine Ref.11 Ref.12

Modified residue

537

Phosphoserine Ref.11 Ref.12

Modified residue

538

Phosphothreonine Ref.12

Sequences

Sequence

Length

Mass (Da)

Tools

P12709 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 556D3D3B77EED052
Show »

FASTA

554

61,299

        10         20         30         40         50         60 
MSNNSFTNFK LATELPAWSK LQKIYESQGK TLSVKQEFQK DAKRFEKLNK TFTNYDGSKI 

        70         80         90        100        110        120 
LFDYSKNLVN DEIIAALIEL AKEANVTGLR DAMFKGEHIN STEDRAVYHV ALRNRANKPM 

       130        140        150        160        170        180 
YVDGVNVAPE VDSVLKHMKE FSEQVRSGEW KGYTGKKITD VVNIGIGGSD LGPVMVTEAL 

       190        200        210        220        230        240 
KHYAGVLDVH FVSNIDGTHI AETLKVVDPE TTLFLIASKT FTTAETITNA NTAKNWFLSK 

       250        260        270        280        290        300 
TGNDPSHIAK HFAALSTNET EVAKFGIDTK NMFGFESWVG GRYSVWSAIG LSVALYIGYD 

       310        320        330        340        350        360 
NFEAFLKGAE AVDNHFTQTP LEDNIPLLGG LLSVWYNNFF GAQTHLVAPF DQYLHRFPAY 

       370        380        390        400        410        420 
LQQLSMESNG KSVTRGNVFT DYSTGSILFG EPATNAQHSF FQLVHQGTKL IPSDFILAAQ 

       430        440        450        460        470        480 
SHNPIENKLH QKMLASNFFA QAEALMVGKD EEQVKAEGAT GGLVPHKVFS GNRPTTSILA 

       490        500        510        520        530        540 
QKITPATLGA LIAYYEHVTF TEGAIWNINS FDQWGVELGK VLAKVIGKEL DNSSTISTHD 

       550 
ASTNGLINQF KEWM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation, characterization and nucleotide sequence of the phosphoglucoisomerase gene of Saccharomyces cerevisiae." Tekamp-Olson P., Najarian R., Burke R.L. Gene 73:153-161(1988) [PubMed: 3072254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae." Green J.B.A., Wright A.P.H., Cheung W.Y., Lancashire W.E., Hartley B.S. Mol. Gen. Genet. 215:100-106(1988) [PubMed: 3071735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[3]	"RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif." Demolis N., Mallet L., Bussereau F., Jacquet M. Yeast 9:645-659(1993) [PubMed: 8346681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family." Mallet L., Bussereau F., Jacquet M. Yeast 10:819-831(1994) [PubMed: 7975899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 424-554. Strain: ATCC 204508 / S288c.
[7]	"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT SER-2.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193 AND THR-198, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-534 AND SER-537, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-51; THR-53; THR-102; THR-223; SER-470; SER-534; SER-537 AND THR-538, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37267 Genomic DNA. Translation: AAA34862.1. M21696 Genomic DNA. Translation: AAA34894.1. X13977 Genomic DNA. Translation: CAA32158.1. Z21487 Genomic DNA. Translation: CAA79683.1. Z36065 Genomic DNA. Translation: CAA85158.1. BK006936 Genomic DNA. Translation: DAA07310.1.
PIR	NUBY. JT0484.
RefSeq	NP_009755.1. NM_001178544.1.
3D structure databases
ProteinModelPortal	P12709.
SMR	P12709. Positions 6-553.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1605N.
IntAct	P12709. 15 interactions.
MINT	MINT-386473.
STRING	P12709.
Proteomic databases
PeptideAtlas	P12709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR196C; YBR196C; YBR196C.
GeneID	852495.
KEGG	sce:YBR196C.
NMPDR	fig\|4932.3.peg.463.
Organism-specific databases
SGD	S000000400. PGI1.
Phylogenomic databases
eggNOG	fuNOG04321.
GeneTree	EFGT00050000004334.
HOGENOM	HBG352954.
OMA	GPKIVSQ.
OrthoDB	EOG43NBN0.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-6121.
Gene expression databases
ArrayExpress	P12709.
Genevestigator	P12709.
GermOnline	YBR196C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001672. G6P_Isomerase. IPR023096. G6P_Isomerase_C. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1390.10. G6P_Isomerase_C. 1 hit.
KO	K01810.
PANTHER	PTHR11469. G6P_Isomerase. 1 hit.
Pfam	PF00342. PGI. 1 hit. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971489.

Entry information

Entry name

G6PI_YEAST

Accession

Primary (citable) accession number: P12709
Secondary accession number(s): D6VQJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents