Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Insulin-1

Gene

ins-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-1
Cleaved into the following 2 chains:
Gene namesi
Name:ins-a
Synonyms:ins1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252363. ins.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Peptidei24 – 5330Insulin-1 B chainPRO_0000015929Add
BLAST
Propeptidei56 – 8328C peptidePRO_0000015930Add
BLAST
Peptidei86 – 10621Insulin-1 A chainPRO_0000015931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 92Interchain (between B and A chains)
Disulfide bondi42 ↔ 105Interchain (between B and A chains)
Disulfide bondi91 ↔ 96

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Structurei

3D structure databases

ProteinModelPortaliP12706.
SMRiP12706. Positions 24-52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006137.
KOiK04526.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALWMQCLPL VLVLFFSTPN TEALVNQHLC GSHLVEALYL VCGDRGFFYY
60 70 80 90 100
PKVKRDMEQA LVSGPQDNEL DGMQLQPQEY QKMKRGIVEQ CCHSTCSLFQ

LESYCN
Length:106
Mass (Da):12,170
Last modified:April 1, 1990 - v2
Checksum:iE3552E865CCF025A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24443 mRNA. Translation: AAA49888.1.
PIRiA33847. IPXL1.
RefSeqiNP_001079351.1. NM_001085882.1.
UniGeneiXl.817.

Genome annotation databases

GeneIDi378696.
KEGGixla:378696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24443 mRNA. Translation: AAA49888.1.
PIRiA33847. IPXL1.
RefSeqiNP_001079351.1. NM_001085882.1.
UniGeneiXl.817.

3D structure databases

ProteinModelPortaliP12706.
SMRiP12706. Positions 24-52.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi378696.
KEGGixla:378696.

Organism-specific databases

CTDi378696.
XenbaseiXB-GENE-6252363. ins.

Phylogenomic databases

HOVERGENiHBG006137.
KOiK04526.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR004825. Insulin.
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00277. INSULIN.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective."
    Shuldiner A.R., Phillips S., Roberts C.T. Jr., Leroith D., Roth J.
    J. Biol. Chem. 264:9428-9432(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of two different insulins from an amphibian, Xenopus laevis."
    Shuldiner A.R., Bennett C., Robinson E.A., Roth J.
    Endocrinology 125:469-477(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-53 AND 86-106.

Entry informationi

Entry nameiINS1_XENLA
AccessioniPrimary (citable) accession number: P12706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: April 1, 1990
Last modified: October 14, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.