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P12689

- REV1_YEAST

UniProt

P12689 - REV1_YEAST

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Protein

DNA repair protein REV1

Gene

REV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.3 Publications

Cofactori

Binds 2 magnesium ions.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei324 – 3241dCTP2 Publications
Metal bindingi362 – 3621Magnesium 11 PublicationPROSITE-ProRule annotation
Metal bindingi362 – 3621Magnesium 21 PublicationPROSITE-ProRule annotation
Metal bindingi363 – 3631Magnesium 2; via carbonyl oxygen1 PublicationPROSITE-ProRule annotation
Binding sitei414 – 4141dCTP2 Publications
Metal bindingi467 – 4671Magnesium 11 PublicationPROSITE-ProRule annotation
Binding sitei467 – 4671dCTP2 Publications
Metal bindingi468 – 4681Magnesium 11 PublicationPROSITE-ProRule annotation
Sitei681 – 6811Interaction with target DNA
Sitei692 – 6921Interaction with target DNA
Sitei694 – 6941Interaction with target DNA

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi362 – 3665dCTP binding2 Publications
Nucleotide bindingi402 – 4087dCTP binding2 Publications

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. deoxycytidyl transferase activity Source: SGD
  3. DNA-directed DNA polymerase activity Source: SGD
  4. magnesium ion binding Source: InterPro

GO - Biological processi

  1. DNA-dependent DNA replication Source: GOC
  2. error-free translesion synthesis Source: SGD
  3. error-prone translesion synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33819-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein REV1 (EC:2.7.7.-)
Alternative name(s):
Reversionless protein 1
Gene namesi
Name:REV1
Ordered Locus Names:YOR346W
ORF Names:O6339
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR346w.
SGDiS000005873. REV1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: SGD
  2. nucleus Source: UniProtKB-KW
  3. replication fork Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi193 – 1931G → R: Loss of activity. 1 Publication
Mutagenesisi467 – 4682DE → AA: Loss of dCTP transferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 985985DNA repair protein REV1PRO_0000173995Add
BLAST

Proteomic databases

MaxQBiP12689.
PaxDbiP12689.

Expressioni

Gene expression databases

GenevestigatoriP12689.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
REV7P389273EBI-14951,EBI-14960

Protein-protein interaction databases

BioGridi34731. 56 interactions.
DIPiDIP-1000N.
IntActiP12689. 4 interactions.
MINTiMINT-2732340.
STRINGi4932.YOR346W.

Structurei

Secondary structure

1
985
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni165 – 1684
Beta strandi170 – 1734
Helixi181 – 19010
Beta strandi194 – 1985
Turni202 – 2043
Beta strandi207 – 2093
Helixi215 – 2206
Turni221 – 2233
Beta strandi224 – 2274
Helixi230 – 2389
Helixi244 – 2474
Helixi315 – 3228
Helixi324 – 34219
Turni343 – 3453
Beta strandi355 – 3639
Turni364 – 3674
Helixi368 – 3736
Helixi377 – 3793
Turni384 – 3863
Beta strandi389 – 3913
Beta strandi395 – 3973
Beta strandi401 – 4033
Helixi405 – 4095
Helixi418 – 4225
Beta strandi432 – 4343
Helixi438 – 45417
Beta strandi459 – 4657
Beta strandi468 – 4758
Helixi485 – 50117
Beta strandi506 – 5138
Helixi514 – 52411
Helixi534 – 5363
Helixi539 – 5424
Helixi547 – 5493
Helixi555 – 56410
Helixi571 – 5777
Helixi580 – 5878
Helixi591 – 5988
Turni599 – 6013
Helixi605 – 6128
Helixi614 – 6185
Beta strandi623 – 6275
Helixi636 – 65621
Beta strandi659 – 67113
Beta strandi687 – 70115
Helixi704 – 71815
Helixi722 – 7243
Beta strandi725 – 73713

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AQ4X-ray2.32A305-738[»]
2M2INMR-A158-251[»]
3BJYX-ray2.41A305-738[»]
3OSPX-ray2.50A305-738[»]
4ID3X-ray1.97A/B159-250[»]
ProteinModelPortaliP12689.
SMRiP12689. Positions 158-251, 305-738, 806-831.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12689.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 24989BRCTPROSITE-ProRule annotationAdd
BLAST
Domaini358 – 554197UmuCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni319 – 32911Interaction with target DNAAdd
BLAST
Regioni395 – 3973Interaction with target DNA
Regioni554 – 5574Interaction with target DNA
Regioni620 – 6289Interaction with target DNA

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0389.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000141717.
InParanoidiP12689.
KOiK03515.
OMAiDCFFATV.
OrthoDBiEOG783N3S.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR012112. REV1.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036573. REV1. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12689-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGEHGGLVDL LDSDLEYSIN RETPDKNNCL SQQSVNDSHL TAKTGGLNAR
60 70 80 90 100
SFLSTLSDDS LIEYVNQLSQ TNKNNSNPTA GTLRFTTKNI SCDELHADLG
110 120 130 140 150
GGEDSPIARS VIEIQESDSN GDDVKKNTVY TREAYFHEKA HGQTLQDQIL
160 170 180 190 200
KDQYKDQISS QSSKIFKNCV IYINGYTKPG RLQLHEMIVL HGGKFLHYLS
210 220 230 240 250
SKKTVTHIVA SNLPLKKRIE FANYKVVSPD WIVDSVKEAR LLPWQNYSLT
260 270 280 290 300
SKLDEQQKKL DNCKTVNSIP LPSETSLHKG SKCVGSALLP VEQQSPVNLN
310 320 330 340 350
NLEAKRIVAC DDPDFLTSYF AHSRLHHLSA WKANLKDKFL NENIHKYTKI
360 370 380 390 400
TDKDTYIIFH IDFDCFFATV AYLCRSSSFS ACDFKRDPIV VCHGTKNSDI
410 420 430 440 450
ASCNYVARSY GIKNGMWVSQ AEKMLPNGIK LISLPYTFEQ FQLKSEAFYS
460 470 480 490 500
TLKRLNIFNL ILPISIDEAV CVRIIPDNIH NTNTLNARLC EEIRQEIFQG
510 520 530 540 550
TNGCTVSIGC SDSLVLARLA LKMAKPNGYN ITFKSNLSEE FWSSFKLDDL
560 570 580 590 600
PGVGHSTLSR LESTFDSPHS LNDLRKRYTL DALKASVGSK LGMKIHLALQ
610 620 630 640 650
GQDDEESLKI LYDPKEVLQR KSLSIDINWG IRFKNITQVD LFIERGCQYL
660 670 680 690 700
LEKLNEINKT TSQITLKLMR RCKDAPIEPP KYMGMGRCDS FSRSSRLGIP
710 720 730 740 750
TNEFGIIATE MKSLYRTLGC PPMELRGLAL QFNKLVDVGP DNNQLKLRLP
760 770 780 790 800
FKTIVTNRAF EALPEDVKND INNEFEKRNY KRKESGLTSN SLSSKKKGFA
810 820 830 840 850
ISRLEVNDLP STMEEQFMNE LPTQIRAEVR HDLRIQKKIQ QTKLGNLQEK
860 870 880 890 900
IKRREESLQN EKNHFMGQNS IFQPIKFQNL TRFKKICQLV KQWVAETLGD
910 920 930 940 950
GGPHEKDVKL FVKYLIKLCD SNRVHLVLHL SNLISRELNL CAFLNQDHSG
960 970 980
FQTWERILLN DIIPLLNRNK HTYQTVRKLD MDFEV
Length:985
Mass (Da):112,227
Last modified:November 1, 1997 - v2
Checksum:i330E30A27F8300BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti646 – 6461G → S in AAA34967. (PubMed:2492497)Curated
Sequence conflicti816 – 8161Q → H in AAA34967. (PubMed:2492497)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22222 Genomic DNA. Translation: AAA34967.1.
X95720 Genomic DNA. Translation: CAA65033.1.
Z75253 Genomic DNA. Translation: CAA99674.1.
BK006948 Genomic DNA. Translation: DAA11107.1.
PIRiS67255.
RefSeqiNP_014991.1. NM_001183766.1.

Genome annotation databases

EnsemblFungiiYOR346W; YOR346W; YOR346W.
GeneIDi854527.
KEGGisce:YOR346W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22222 Genomic DNA. Translation: AAA34967.1 .
X95720 Genomic DNA. Translation: CAA65033.1 .
Z75253 Genomic DNA. Translation: CAA99674.1 .
BK006948 Genomic DNA. Translation: DAA11107.1 .
PIRi S67255.
RefSeqi NP_014991.1. NM_001183766.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AQ4 X-ray 2.32 A 305-738 [» ]
2M2I NMR - A 158-251 [» ]
3BJY X-ray 2.41 A 305-738 [» ]
3OSP X-ray 2.50 A 305-738 [» ]
4ID3 X-ray 1.97 A/B 159-250 [» ]
ProteinModelPortali P12689.
SMRi P12689. Positions 158-251, 305-738, 806-831.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34731. 56 interactions.
DIPi DIP-1000N.
IntActi P12689. 4 interactions.
MINTi MINT-2732340.
STRINGi 4932.YOR346W.

Proteomic databases

MaxQBi P12689.
PaxDbi P12689.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR346W ; YOR346W ; YOR346W .
GeneIDi 854527.
KEGGi sce:YOR346W.

Organism-specific databases

CYGDi YOR346w.
SGDi S000005873. REV1.

Phylogenomic databases

eggNOGi COG0389.
GeneTreei ENSGT00530000062942.
HOGENOMi HOG000141717.
InParanoidi P12689.
KOi K03515.
OMAi DCFFATV.
OrthoDBi EOG783N3S.

Enzyme and pathway databases

BioCyci YEAST:G3O-33819-MONOMER.

Miscellaneous databases

EvolutionaryTracei P12689.
NextBioi 976907.
PROi P12689.

Gene expression databases

Genevestigatori P12689.

Family and domain databases

Gene3Di 3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR012112. REV1.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF036573. REV1. 1 hit.
SMARTi SM00292. BRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis."
    Larimer F.W., Perry J.R., Hardigree A.A.
    J. Bacteriol. 171:230-237(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-193.
  2. "Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes."
    Purnelle B., Goffeau A.
    Yeast 12:1475-1481(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90843 / S288c / FY73.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Deoxycytidyl transferase activity of yeast REV1 protein."
    Nelson J.R., Lawrence C.W., Hinkle D.C.
    Nature 382:729-731(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Roles of yeast DNA polymerases delta and zeta and of Rev1 in the bypass of abasic sites."
    Haracska L., Unk I., Johnson R.E., Johansson E., Burgers P.M.J., Prakash S., Prakash L.
    Genes Dev. 15:945-954(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 467-ASP-GLU-468.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Saccharomyces cerevisiae polymerase zeta functions in mitochondria."
    Zhang H., Chatterjee A., Singh K.K.
    Genetics 172:2683-2688(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "Rev1 employs a novel mechanism of DNA synthesis using a protein template."
    Nair D.T., Johnson R.E., Prakash L., Prakash S., Aggarwal A.K.
    Science 309:2219-2222(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 305-738 IN COMPLEX WITH MAGNESIUM IONS; DNA AND DCTP, COFACTOR.
  11. "Protein-template-directed synthesis across an acrolein-derived DNA adduct by yeast Rev1 DNA polymerase."
    Nair D.T., Johnson R.E., Prakash L., Prakash S., Aggarwal A.K.
    Structure 16:239-245(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 305-738 IN COMPLEX WITH MAGNESIUM; DCTP AND TARGET DNA, COFACTOR.

Entry informationi

Entry nameiREV1_YEAST
AccessioniPrimary (citable) accession number: P12689
Secondary accession number(s): D6W341, Q12323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3