ID   RM02_YEAST              Reviewed;         371 AA.
AC   P12687; D6W1H2;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   27-MAR-2024, entry version 201.
DE   RecName: Full=Large ribosomal subunit protein bL27m {ECO:0000303|PubMed:24675956};
DE   AltName: Full=54S ribosomal protein L2, mitochondrial;
DE   AltName: Full=YMR6;
DE   AltName: Full=YmL2;
DE   Flags: Precursor;
GN   Name=MRP7; Synonyms=MRPL2; OrderedLocusNames=YNL005C; ORFNames=N2007;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 26109 / X2180 / NCYC 826;
RX   PubMed=2851722; DOI=10.1128/mcb.8.9.3636-3646.1988;
RA   Fearon K., Mason T.L.;
RT   "Structure and regulation of a nuclear gene in Saccharomyces cerevisiae
RT   that specifies MRP7, a protein of the large subunit of the mitochondrial
RT   ribosome.";
RL   Mol. Cell. Biol. 8:3636-3646(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / GRF88;
RX   PubMed=7941739; DOI=10.1002/yea.320100412;
RA   Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.;
RT   "Organization of the centromeric region of chromosome XIV in Saccharomyces
RT   cerevisiae.";
RL   Yeast 10:523-533(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=7985421; DOI=10.1002/yea.320100709;
RA   Verhasselt P., Aert R., Voet M., Volckaert G.;
RT   "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast
RT   chromosome XIV, encompassing the centromere sequence.";
RL   Yeast 10:945-951(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [7]
RP   PROTEIN SEQUENCE OF N-TERMINUS.
RX   PubMed=2693936; DOI=10.1007/bf00261166;
RA   Matsushita Y., Kitakawa M., Isono K.;
RT   "Cloning and analysis of the nuclear genes for two mitochondrial ribosomal
RT   proteins in yeast.";
RL   Mol. Gen. Genet. 219:119-124(1989).
RN   [8]
RP   PROTEIN SEQUENCE OF 28-50, AND SUBUNIT.
RC   STRAIN=07173;
RX   PubMed=2060626; DOI=10.1016/0014-5793(91)80759-v;
RA   Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S.,
RA   Kitakawa M.;
RT   "Extended N-terminal sequencing of proteins of the large ribosomal subunit
RT   from yeast mitochondria.";
RL   FEBS Lett. 284:51-56(1991).
RN   [9]
RP   TRANSPORT INTO MITOCHONDRIA.
RX   PubMed=8220240;
RA   Matsushita Y., Isono K.;
RT   "Transport of mitoribosomal proteins, YmL13 and MRP7, into isolated
RT   mitochondria of Saccharomyces cerevisiae.";
RL   Biochem. Mol. Biol. Int. 30:911-919(1993).
RN   [10]
RP   IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x;
RA   Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.;
RT   "Tag-mediated isolation of yeast mitochondrial ribosome and mass
RT   spectrometric identification of its new components.";
RL   Eur. J. Biochem. 269:5203-5214(2002).
RN   [11]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [12]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [13]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 76625 / YPH499;
RX   PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA   Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA   Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA   Pfanner N., Meisinger C.;
RT   "The proteome of Saccharomyces cerevisiae mitochondria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN   [14]
RP   SUBCELLULAR LOCATION.
RX   PubMed=25609543; DOI=10.1038/ncomms7019;
RA   Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.;
RT   "Organization of the mitochondrial translation machinery studied in situ by
RT   cryoelectron tomography.";
RL   Nat. Commun. 6:6019-6019(2015).
RN   [15]
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT.
RX   PubMed=24675956; DOI=10.1126/science.1249410;
RA   Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., Long F.,
RA   Murshudov G., Scheres S.H., Ramakrishnan V.;
RT   "Structure of the yeast mitochondrial large ribosomal subunit.";
RL   Science 343:1485-1489(2014).
CC   -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a
CC       dedicated translation machinery responsible for the synthesis of
CC       mitochondrial genome-encoded proteins, including at least some of the
CC       essential transmembrane subunits of the mitochondrial respiratory
CC       chain. The mitoribosomes are attached to the mitochondrial inner
CC       membrane and translation products are cotranslationally integrated into
CC       the membrane. {ECO:0000305|PubMed:24675956,
CC       ECO:0000305|PubMed:25609543}.
CC   -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit (mt-
CC       LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S)
CC       and a large (54S) subunit. The 37S small subunit contains a 15S
CC       ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large
CC       subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.
CC       {ECO:0000269|PubMed:12392552, ECO:0000269|PubMed:2060626,
CC       ECO:0000269|PubMed:24675956}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:14576278}. Note=Mitoribosomes are tethered to the
CC       mitochondrial inner membrane and spatially aligned with the membrane
CC       insertion machinery through two distinct membrane contact sites, formed
CC       by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein
CC       MBA1. {ECO:0000269|PubMed:25609543}.
CC   -!- MISCELLANEOUS: Present with 2300 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL27 family.
CC       {ECO:0000305}.
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DR   EMBL; M22116; AAA34794.1; -; Genomic_DNA.
DR   EMBL; X77114; CAA54379.1; -; Genomic_DNA.
DR   EMBL; Z71281; CAA95864.1; -; Genomic_DNA.
DR   EMBL; AY693088; AAT93107.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10538.1; -; Genomic_DNA.
DR   PIR; S62914; R6BYM7.
DR   RefSeq; NP_014393.1; NM_001182844.1.
DR   PDB; 3J6B; EM; 3.20 A; R=1-371.
DR   PDB; 5MRC; EM; 3.25 A; R=35-371.
DR   PDB; 5MRE; EM; 3.75 A; R=35-371.
DR   PDB; 5MRF; EM; 4.97 A; R=35-371.
DR   PDBsum; 3J6B; -.
DR   PDBsum; 5MRC; -.
DR   PDBsum; 5MRE; -.
DR   PDBsum; 5MRF; -.
DR   AlphaFoldDB; P12687; -.
DR   EMDB; EMD-3551; -.
DR   EMDB; EMD-3552; -.
DR   EMDB; EMD-3553; -.
DR   SMR; P12687; -.
DR   BioGRID; 35820; 254.
DR   ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit.
DR   DIP; DIP-5620N; -.
DR   IntAct; P12687; 15.
DR   MINT; P12687; -.
DR   STRING; 4932.YNL005C; -.
DR   iPTMnet; P12687; -.
DR   MaxQB; P12687; -.
DR   PaxDb; 4932-YNL005C; -.
DR   PeptideAtlas; P12687; -.
DR   EnsemblFungi; YNL005C_mRNA; YNL005C; YNL005C.
DR   GeneID; 855727; -.
DR   KEGG; sce:YNL005C; -.
DR   AGR; SGD:S000004950; -.
DR   SGD; S000004950; MRP7.
DR   VEuPathDB; FungiDB:YNL005C; -.
DR   eggNOG; KOG4600; Eukaryota.
DR   GeneTree; ENSGT00960000189216; -.
DR   HOGENOM; CLU_063752_0_0_1; -.
DR   InParanoid; P12687; -.
DR   OMA; YLDPFHP; -.
DR   OrthoDB; 179811at2759; -.
DR   BioCyc; YEAST:G3O-33047-MONOMER; -.
DR   BioGRID-ORCS; 855727; 6 hits in 10 CRISPR screens.
DR   PRO; PR:P12687; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P12687; Protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; NAS:ComplexPortal.
DR   GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0032543; P:mitochondrial translation; NAS:ComplexPortal.
DR   Gene3D; 2.40.50.100; -; 1.
DR   InterPro; IPR001684; Ribosomal_bL27.
DR   InterPro; IPR018261; Ribosomal_bL27_CS.
DR   InterPro; IPR041244; Ribosomal_bL27m_C.
DR   NCBIfam; TIGR00062; L27; 1.
DR   PANTHER; PTHR15893:SF0; 39S RIBOSOMAL PROTEIN L27, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR15893; RIBOSOMAL PROTEIN L27; 1.
DR   Pfam; PF01016; Ribosomal_L27; 1.
DR   Pfam; PF18471; Ribosomal_L27_C; 1.
DR   PRINTS; PR00063; RIBOSOMALL27.
DR   SUPFAM; SSF110324; Ribosomal L27 protein-like; 1.
DR   PROSITE; PS00831; RIBOSOMAL_L27; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Mitochondrion; Reference proteome;
KW   Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT   TRANSIT         1..27
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:2060626,
FT                   ECO:0000269|PubMed:2693936"
FT   CHAIN           28..371
FT                   /note="Large ribosomal subunit protein bL27m"
FT                   /id="PRO_0000030503"
FT   CONFLICT        28..29
FT                   /note="AT -> SG (in Ref. 7; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        350
FT                   /note="F -> L (in Ref. 3; CAA54379)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   371 AA;  43227 MW;  F8F17EF955BB7ACF CRC64;
     MWNPILLDTS SFSFQKHVSG VFLQVRNATK RAAGSRTSMK DSAGRRLGPK KYEGQDVSTG
     EIIMRQRGTK FYPGENVGIG KDHSIFALEP GVVRYYLDPF HPKRKFIGVA LRRDLKLPSP
     HFEPTVRRFG RFELTNKRAA YKEENSISRK DYLAKPNILK QLEVRESKRK ELQDKLSKVL
     RDELKLDIKD IELATSYLIR VRASLKNGYP IEDARFNSRY YLKEEERLKA RRESWTNEKL
     SESLSKIDEC SDLLNSSTSF NNKLELHQYI SEQEKQALKA KLLEDLEKSQ HLETKKDKNY
     IKALFKDACN FLTLSEEVHL RRKYLKSVFP ETDSTVETKS GKKSIVSRRF DYTKNKVEVI
     ARSRRAFLSK L
//