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P12687

- RM02_YEAST

UniProt

P12687 - RM02_YEAST

Protein

54S ribosomal protein L2, mitochondrial

Gene

MRP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. peptidyltransferase activity Source: SGD
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33047-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    54S ribosomal protein L2, mitochondrial
    Alternative name(s):
    YMR6
    YmL2
    Gene namesi
    Name:MRP7
    Synonyms:MRPL2
    Ordered Locus Names:YNL005C
    ORF Names:N2007
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL005c.
    SGDiS000004950. MRP7.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrial large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727Mitochondrion2 PublicationsAdd
    BLAST
    Chaini28 – 37134454S ribosomal protein L2, mitochondrialPRO_0000030503Add
    BLAST

    Proteomic databases

    MaxQBiP12687.
    PaxDbiP12687.
    PeptideAtlasiP12687.

    Expressioni

    Gene expression databases

    GenevestigatoriP12687.

    Interactioni

    Subunit structurei

    Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).2 Publications

    Protein-protein interaction databases

    BioGridi35820. 45 interactions.
    DIPiDIP-5620N.
    IntActiP12687. 13 interactions.
    MINTiMINT-476381.
    STRINGi4932.YNL005C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW4electron microscopy3.20R1-371[»]
    ProteinModelPortaliP12687.
    SMRiP12687. Positions 46-95.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L27P family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0211.
    GeneTreeiENSGT00390000014660.
    HOGENOMiHOG000248704.
    OMAiYLDPFHP.
    OrthoDBiEOG75MW7P.

    Family and domain databases

    InterProiIPR001684. Ribosomal_L27.
    IPR018261. Ribosomal_L27_CS.
    [Graphical view]
    PANTHERiPTHR15893. PTHR15893. 1 hit.
    PfamiPF01016. Ribosomal_L27. 1 hit.
    [Graphical view]
    PRINTSiPR00063. RIBOSOMALL27.
    ProDomiPD003114. Ribosomal_L27. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    TIGRFAMsiTIGR00062. L27. 1 hit.
    PROSITEiPS00831. RIBOSOMAL_L27. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12687-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWNPILLDTS SFSFQKHVSG VFLQVRNATK RAAGSRTSMK DSAGRRLGPK    50
    KYEGQDVSTG EIIMRQRGTK FYPGENVGIG KDHSIFALEP GVVRYYLDPF 100
    HPKRKFIGVA LRRDLKLPSP HFEPTVRRFG RFELTNKRAA YKEENSISRK 150
    DYLAKPNILK QLEVRESKRK ELQDKLSKVL RDELKLDIKD IELATSYLIR 200
    VRASLKNGYP IEDARFNSRY YLKEEERLKA RRESWTNEKL SESLSKIDEC 250
    SDLLNSSTSF NNKLELHQYI SEQEKQALKA KLLEDLEKSQ HLETKKDKNY 300
    IKALFKDACN FLTLSEEVHL RRKYLKSVFP ETDSTVETKS GKKSIVSRRF 350
    DYTKNKVEVI ARSRRAFLSK L 371
    Length:371
    Mass (Da):43,227
    Last modified:October 1, 1989 - v1
    Checksum:iF8F17EF955BB7ACF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 292AT → SG AA sequence (PubMed:2693936)Curated
    Sequence conflicti350 – 3501F → L in CAA54379. (PubMed:7985421)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22116 Genomic DNA. Translation: AAA34794.1.
    X77114 Genomic DNA. Translation: CAA54379.1.
    Z71281 Genomic DNA. Translation: CAA95864.1.
    AY693088 Genomic DNA. Translation: AAT93107.1.
    BK006947 Genomic DNA. Translation: DAA10538.1.
    PIRiS62914. R6BYM7.
    RefSeqiNP_014393.1. NM_001182844.1.

    Genome annotation databases

    EnsemblFungiiYNL005C; YNL005C; YNL005C.
    GeneIDi855727.
    KEGGisce:YNL005C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22116 Genomic DNA. Translation: AAA34794.1 .
    X77114 Genomic DNA. Translation: CAA54379.1 .
    Z71281 Genomic DNA. Translation: CAA95864.1 .
    AY693088 Genomic DNA. Translation: AAT93107.1 .
    BK006947 Genomic DNA. Translation: DAA10538.1 .
    PIRi S62914. R6BYM7.
    RefSeqi NP_014393.1. NM_001182844.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW4 electron microscopy 3.20 R 1-371 [» ]
    ProteinModelPortali P12687.
    SMRi P12687. Positions 46-95.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35820. 45 interactions.
    DIPi DIP-5620N.
    IntActi P12687. 13 interactions.
    MINTi MINT-476381.
    STRINGi 4932.YNL005C.

    Proteomic databases

    MaxQBi P12687.
    PaxDbi P12687.
    PeptideAtlasi P12687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL005C ; YNL005C ; YNL005C .
    GeneIDi 855727.
    KEGGi sce:YNL005C.

    Organism-specific databases

    CYGDi YNL005c.
    SGDi S000004950. MRP7.

    Phylogenomic databases

    eggNOGi COG0211.
    GeneTreei ENSGT00390000014660.
    HOGENOMi HOG000248704.
    OMAi YLDPFHP.
    OrthoDBi EOG75MW7P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33047-MONOMER.

    Miscellaneous databases

    NextBioi 980102.
    PROi P12687.

    Gene expression databases

    Genevestigatori P12687.

    Family and domain databases

    InterProi IPR001684. Ribosomal_L27.
    IPR018261. Ribosomal_L27_CS.
    [Graphical view ]
    PANTHERi PTHR15893. PTHR15893. 1 hit.
    Pfami PF01016. Ribosomal_L27. 1 hit.
    [Graphical view ]
    PRINTSi PR00063. RIBOSOMALL27.
    ProDomi PD003114. Ribosomal_L27. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    TIGRFAMsi TIGR00062. L27. 1 hit.
    PROSITEi PS00831. RIBOSOMAL_L27. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and regulation of a nuclear gene in Saccharomyces cerevisiae that specifies MRP7, a protein of the large subunit of the mitochondrial ribosome."
      Fearon K., Mason T.L.
      Mol. Cell. Biol. 8:3636-3646(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26109 / X2180 / NCYC 826.
    2. "Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae."
      Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.
      Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    3. "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence."
      Verhasselt P., Aert R., Voet M., Volckaert G.
      Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast."
      Matsushita Y., Kitakawa M., Isono K.
      Mol. Gen. Genet. 219:119-124(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
    8. "Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
      Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
      FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-50, SUBUNIT.
      Strain: 07173.
    9. "Transport of mitoribosomal proteins, YmL13 and MRP7, into isolated mitochondria of Saccharomyces cerevisiae."
      Matsushita Y., Isono K.
      Biochem. Mol. Biol. Int. 30:911-919(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSPORT INTO MITOCHONDRIA.
    10. "Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
      Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
      Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiRM02_YEAST
    AccessioniPrimary (citable) accession number: P12687
    Secondary accession number(s): D6W1H2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3