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P12687 (RM02_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
54S ribosomal protein L2, mitochondrial
Alternative name(s):
YMR6
YmL2
Gene names
Name:MRP7
Synonyms:MRPL2
Ordered Locus Names:YNL005C
ORF Names:N2007
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.8 Ref.10

Subcellular location

Mitochondrion Ref.11 Ref.13.

Miscellaneous

Present with 2300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ribosomal protein L27P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Ref.7 Ref.8
Chain28 – 37134454S ribosomal protein L2, mitochondrial
PRO_0000030503

Experimental info

Sequence conflict28 – 292AT → SG AA sequence Ref.7
Sequence conflict3501F → L in CAA54379. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P12687 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: F8F17EF955BB7ACF

FASTA37143,227
        10         20         30         40         50         60 
MWNPILLDTS SFSFQKHVSG VFLQVRNATK RAAGSRTSMK DSAGRRLGPK KYEGQDVSTG 

        70         80         90        100        110        120 
EIIMRQRGTK FYPGENVGIG KDHSIFALEP GVVRYYLDPF HPKRKFIGVA LRRDLKLPSP 

       130        140        150        160        170        180 
HFEPTVRRFG RFELTNKRAA YKEENSISRK DYLAKPNILK QLEVRESKRK ELQDKLSKVL 

       190        200        210        220        230        240 
RDELKLDIKD IELATSYLIR VRASLKNGYP IEDARFNSRY YLKEEERLKA RRESWTNEKL 

       250        260        270        280        290        300 
SESLSKIDEC SDLLNSSTSF NNKLELHQYI SEQEKQALKA KLLEDLEKSQ HLETKKDKNY 

       310        320        330        340        350        360 
IKALFKDACN FLTLSEEVHL RRKYLKSVFP ETDSTVETKS GKKSIVSRRF DYTKNKVEVI 

       370 
ARSRRAFLSK L 

« Hide

References

« Hide 'large scale' references
[1]"Structure and regulation of a nuclear gene in Saccharomyces cerevisiae that specifies MRP7, a protein of the large subunit of the mitochondrial ribosome."
Fearon K., Mason T.L.
Mol. Cell. Biol. 8:3636-3646(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26109 / X2180 / NCYC 826.
[2]"Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae."
Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.
Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[3]"Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence."
Verhasselt P., Aert R., Voet M., Volckaert G.
Yeast 10:945-951(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast."
Matsushita Y., Kitakawa M., Isono K.
Mol. Gen. Genet. 219:119-124(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS.
[8]"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria."
Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M.
FEBS Lett. 284:51-56(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-50, SUBUNIT.
Strain: 07173.
[9]"Transport of mitoribosomal proteins, YmL13 and MRP7, into isolated mitochondria of Saccharomyces cerevisiae."
Matsushita Y., Isono K.
Biochem. Mol. Biol. Int. 30:911-919(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSPORT INTO MITOCHONDRIA.
[10]"Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components."
Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.
Eur. J. Biochem. 269:5203-5214(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[11]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22116 Genomic DNA. Translation: AAA34794.1.
X77114 Genomic DNA. Translation: CAA54379.1.
Z71281 Genomic DNA. Translation: CAA95864.1.
AY693088 Genomic DNA. Translation: AAT93107.1.
BK006947 Genomic DNA. Translation: DAA10538.1.
PIRR6BYM7. S62914.
RefSeqNP_014393.1. NM_001182844.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW4electron microscopy3.20R1-371[»]
ProteinModelPortalP12687.
SMRP12687. Positions 46-95.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35820. 45 interactions.
DIPDIP-5620N.
IntActP12687. 13 interactions.
MINTMINT-476381.
STRING4932.YNL005C.

Proteomic databases

MaxQBP12687.
PaxDbP12687.
PeptideAtlasP12687.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL005C; YNL005C; YNL005C.
GeneID855727.
KEGGsce:YNL005C.

Organism-specific databases

CYGDYNL005c.
SGDS000004950. MRP7.

Phylogenomic databases

eggNOGCOG0211.
GeneTreeENSGT00390000014660.
HOGENOMHOG000248704.
OMAYLDPFHP.
OrthoDBEOG75MW7P.

Enzyme and pathway databases

BioCycYEAST:G3O-33047-MONOMER.

Gene expression databases

GenevestigatorP12687.

Family and domain databases

InterProIPR001684. Ribosomal_L27.
IPR018261. Ribosomal_L27_CS.
[Graphical view]
PANTHERPTHR15893. PTHR15893. 1 hit.
PfamPF01016. Ribosomal_L27. 1 hit.
[Graphical view]
PRINTSPR00063. RIBOSOMALL27.
ProDomPD003114. Ribosomal_L27. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00062. L27. 1 hit.
PROSITEPS00831. RIBOSOMAL_L27. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980102.
PROP12687.

Entry information

Entry nameRM02_YEAST
AccessionPrimary (citable) accession number: P12687
Secondary accession number(s): D6W1H2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references