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Protein

High-affinity potassium transport protein

Gene

TRK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is required for high-affinity potassium transport.

GO - Molecular functioni

  • potassium ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • cellular potassium ion homeostasis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciYEAST:G3O-31579-MONOMER.

Protein family/group databases

TCDBi2.A.38.2.1. the k(+) transporter (trk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity potassium transport protein
Gene namesi
Name:TRK1
Ordered Locus Names:YJL129C
ORF Names:J0693
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL129C.
SGDiS000003665. TRK1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei49 – 7022HelicalSequence analysisAdd
BLAST
Transmembranei78 – 9821HelicalSequence analysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST
Transmembranei778 – 80023HelicalSequence analysisAdd
BLAST
Transmembranei813 – 83422HelicalSequence analysisAdd
BLAST
Transmembranei838 – 85821HelicalSequence analysisAdd
BLAST
Transmembranei862 – 88221HelicalSequence analysisAdd
BLAST
Transmembranei898 – 91821HelicalSequence analysisAdd
BLAST
Transmembranei923 – 94321HelicalSequence analysisAdd
BLAST
Transmembranei971 – 99121HelicalSequence analysisAdd
BLAST
Transmembranei1078 – 109821HelicalSequence analysisAdd
BLAST
Transmembranei1111 – 113121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: InterPro
  • membrane raft Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12351235High-affinity potassium transport proteinPRO_0000070462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151PhosphoserineCombined sources
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence analysis
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence analysis
Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence analysis
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence analysis
Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence analysis
Modified residuei414 – 4141PhosphoserineCombined sources
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence analysis
Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence analysis
Modified residuei534 – 5341PhosphoserineCombined sources
Glycosylationi580 – 5801N-linked (GlcNAc...)Sequence analysis
Glycosylationi677 – 6771N-linked (GlcNAc...)Sequence analysis
Glycosylationi919 – 9191N-linked (GlcNAc...)Sequence analysis
Glycosylationi1030 – 10301N-linked (GlcNAc...)Sequence analysis
Glycosylationi1135 – 11351N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

PTM databases

iPTMnetiP12685.

Interactioni

Protein-protein interaction databases

BioGridi33627. 55 interactions.
DIPiDIP-7454N.
IntActiP12685. 1 interaction.
MINTiMINT-2784460.

Structurei

3D structure databases

ProteinModelPortaliP12685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrkH potassium transport family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000069355.
HOGENOMiHOG000161114.
InParanoidiP12685.
OMAiNITDWIL.
OrthoDBiEOG75MW52.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004773. K/Na_transp_Trk/HKT.
IPR015958. Ktransp_fun.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF002450. K+_transpter_TRK. 1 hit.
TIGRFAMsiTIGR00934. 2a38euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P12685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFRRTMSRV PTLASLEIRY KKSFGHKFRD FIALCGHYFA PVKKYIFPSF
60 70 80 90 100
IAVHYFYTIS LTLITSILLY PIKNTRYIDT LFLAAGAVTQ GGLNTVDINN
110 120 130 140 150
LSLYQQIVLY IVCCISTPIA VHSCLAFVRL YWFERYFDGI RDSSRRNFKM
160 170 180 190 200
RRTKTILERE LTARTMTKNR TGTQRTSYPR KQAKTDDFQE KLFSGEMVNR
210 220 230 240 250
DEQDSVHSDQ NSHDISRDSS NNNTNHNGSS GSLDDFVKED ETDDNGEYQE
260 270 280 290 300
NNSYSTVGSS SNTVADESLN QKPKPSSLRF DEPHSKQRPA RVPSEKFAKR
310 320 330 340 350
RGSRDISPAD MYRSIMMLQG KHEATAEDEG PPLVIGSPAD GTRYKSNVNK
360 370 380 390 400
LKKATGINGN KIKIRDKGNE SNTDQNSVSS EANSTASVSD ESSLHTNFGN
410 420 430 440 450
KVPSLRTNTH RSNSGPIAIT DNAETDKKHG PSIQFDITKP PRKISKRVST
460 470 480 490 500
FDDLNPKSSV LYRKKASKKY LMKHFPKARR IRQQIKRRLS TGSIEKNSSN
510 520 530 540 550
NVSDRKPITD MDDDDDDDDN DGDNNEEYFA DNESGDEDER VQQSEPHSDS
560 570 580 590 600
ELKSHQQQQE KHQLQQNLHR MYKTKSFDDN RSRAVPMERS RTIDMAEAKD
610 620 630 640 650
LNELARTPDF QKMVYQNWKA HHRKKPNFRK RGWNNKIFEH GPYASDSDRN
660 670 680 690 700
YPDNSNTGNS ILHYAESILH HDGSHKNGSE EASSDSNENI YSTNGGSDHN
710 720 730 740 750
GLNNYPTYND DEEGYYGLHF DTDYDLDPRH DLSKGSGKTY LSWQPTIGRN
760 770 780 790 800
SNFLGLTRAQ KDELGGVEYR AIKLLCTILV VYYVGWHIVA FVMLVPWIIL
810 820 830 840 850
KKHYSEVVRD DGVSPTWWGF WTAMSAFNDL GLTLTPNSMM SFNKAVYPLI
860 870 880 890 900
VMIWFIIIGN TGFPILLRCI IWIMFKISPD LSQMRESLGF LLDHPRRCFT
910 920 930 940 950
LLFPKAATWW LLLTLAGLNI TDWILFIILD FGSTVVKSLS KGYRVLVGLF
960 970 980 990 1000
QSVSTRTAGF SVVDLSQLHP SIQVSYMLMM YVSVLPLAIS IRRTNVYEEQ
1010 1020 1030 1040 1050
SLGLYGDMGG EPEDTDTEDD GNDEDDDEEN ESHEGQSSQR SSSNNNNNNN
1060 1070 1080 1090 1100
RKKKKKKKTE NPNEISTKSF IGAHLRKQLS FDLWFLFLGL FIICICEGDK
1110 1120 1130 1140 1150
IKDVQEPNFN IFAILFEIVS AYGTVGLSLG YPDTNQSFSR QFTTLSKLVI
1160 1170 1180 1190 1200
IAMLIRGKNR GLPYSLDRAI ILPSDRLEHI DHLEGMKLKR QARTNTEDPM
1210 1220 1230
TEHFKRSFTD VKHRWGALKR KTTHSRNPKR SSTTL
Length:1,235
Mass (Da):141,073
Last modified:October 1, 1989 - v1
Checksum:iBCE9FDBA0BA0982B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21328 Genomic DNA. Translation: AAA34728.1.
Z49404 Genomic DNA. Translation: CAA89424.1.
BK006943 Genomic DNA. Translation: DAA08672.1.
PIRiS05849. PWBYH.
RefSeqiNP_012406.1. NM_001181562.1.

Genome annotation databases

EnsemblFungiiYJL129C; YJL129C; YJL129C.
GeneIDi853312.
KEGGisce:YJL129C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21328 Genomic DNA. Translation: AAA34728.1.
Z49404 Genomic DNA. Translation: CAA89424.1.
BK006943 Genomic DNA. Translation: DAA08672.1.
PIRiS05849. PWBYH.
RefSeqiNP_012406.1. NM_001181562.1.

3D structure databases

ProteinModelPortaliP12685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33627. 55 interactions.
DIPiDIP-7454N.
IntActiP12685. 1 interaction.
MINTiMINT-2784460.

Protein family/group databases

TCDBi2.A.38.2.1. the k(+) transporter (trk) family.

PTM databases

iPTMnetiP12685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL129C; YJL129C; YJL129C.
GeneIDi853312.
KEGGisce:YJL129C.

Organism-specific databases

EuPathDBiFungiDB:YJL129C.
SGDiS000003665. TRK1.

Phylogenomic databases

GeneTreeiENSGT00530000069355.
HOGENOMiHOG000161114.
InParanoidiP12685.
OMAiNITDWIL.
OrthoDBiEOG75MW52.

Enzyme and pathway databases

BioCyciYEAST:G3O-31579-MONOMER.

Miscellaneous databases

NextBioi973651.
PROiP12685.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004773. K/Na_transp_Trk/HKT.
IPR015958. Ktransp_fun.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF002450. K+_transpter_TRK. 1 hit.
TIGRFAMsiTIGR00934. 2a38euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae."
    Gaber R.F., Styles C.A., Fink G.R.
    Mol. Cell. Biol. 8:2848-2859(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon."
    Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C.
    Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-414, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRK1_YEAST
AccessioniPrimary (citable) accession number: P12685
Secondary accession number(s): D6VW56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 11, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.