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P12676 (CHOD_STRS0) Reviewed, UniProtKB/Swiss-Prot

Last modified June 28, 2011. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholesterol oxidase
Short name=CHOD
EC=1.1.3.6
Gene names
Name:choA
OrganismStreptomyces sp. (strain SA-COO)
Taxonomic identifier74576 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Cholesterol + O2 = cholest-4-en-3-one + H2O2.

Cofactor

FAD.

Pathway

Steroid metabolism; cholesterol metabolism.

Subunit structure

Monomer.

Subcellular location

Secreted.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Sequence similarities

Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
   Biological processCholesterol metabolism
Lipid metabolism
Steroid metabolism
   Cellular componentSecreted
   DomainSignal
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological processcholesterol metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncholesterol oxidase activity

Inferred from electronic annotation. Source: EC

flavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4242Tat-type signal
Chain43 – 546504Cholesterol oxidase
PRO_0000012333

Regions

Nucleotide binding54 – 7017FAD Potential

Sites

Active site3981Proton acceptor
Active site4841

Experimental info

Mutagenesis3981E → Q: Reduced activity.
Mutagenesis4841H → N or Q: Reduced activity.

Secondary structure

1 ...................................................................................... 546
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P12676 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: EF22A1FE5EA68D21

FASTA54658,994
        10         20         30         40         50         60 
MTAQQHLSRR RMLGMAAFGA AALAGGTTIA APRAAAAAKS AADNGGYVPA VVIGTGYGAA 

        70         80         90        100        110        120 
VSALRLGEAG VQTLMLEMGQ LWNQPGPDGN IFCGMLNPDK RSSWFKNRTE APLGSFLWLD 

       130        140        150        160        170        180 
VVNRNIDPYA GVLDRVNYDQ MSVYVGRGVG GGSLVNGGMA VEPKRSYFEE ILPRVDSSEM 

       190        200        210        220        230        240 
YDRYFPRANS MLRVNHIDTK WFEDTEWYKF ARVSREQAGK AGLGTVFVPN VYDFGYMQRE 

       250        260        270        280        290        300 
AAGEVPKSAL ATEVIYGNNH GKQSLDKTYL AAALGTGKVT IQTLHQVKTI RQTKDGGYAL 

       310        320        330        340        350        360 
TVEQKDTDGK LLATKEISCR YLFLGAGSLG STELLVRARD TGTLPNLNSE VGAGWGPNGN 

       370        380        390        400        410        420 
IMTARANHMW NPTGAHQSSI PALGIDAWDN SDSSVFAEIA PMPAGLETWV SLYLAITKNP 

       430        440        450        460        470        480 
QRGTFVYDAA TDRAKLNWTR DQNAPAVNAA KALFDRINKA NGTIYRYDLF GTQLKAFADD 

       490        500        510        520        530        540 
FCYHPLGGCV LGKATDDYGR VAGYKNLYVT DGSLIPGSVG VNPFVTITAL AERNVERIIK 


QDVTAS

« Hide

References

[1]"Nucleotide sequence of the gene for cholesterol oxidase from a Streptomyces sp."
Ishizaki T., Hirayama N., Shinkawa H., Nimi O., Murooka Y.
J. Bacteriol. 171:596-601(1989) [PubMed: 2914858] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants."
Yue Q.K., Kass I.J., Sampson N.S., Vrielink A.
Biochemistry 38:4277-4286(1999) [PubMed: 10194345] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), MUTAGENESIS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M31939 Genomic DNA. Translation: AAA26719.1.
PIRA32260.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B4VX-ray1.50A43-546[»]
1B8SX-ray1.65A43-546[»]
1CBOX-ray1.80A43-546[»]
1CC2X-ray2.20A43-546[»]
1IJHX-ray1.53A43-546[»]
1MXTX-ray0.95A43-546[»]
1N1PX-ray0.95A43-546[»]
1N4UX-ray0.95A43-546[»]
1N4VX-ray1.00A43-546[»]
1N4WX-ray0.92A43-546[»]
2GEWX-ray0.97A43-546[»]
3B3RX-ray0.98A42-546[»]
3B6DX-ray1.20A43-546[»]
3CNJX-ray0.95A45-543[»]
3GYIX-ray1.00A43-546[»]
3GYJX-ray0.92A43-546[»]
ProteinModelPortalP12676.
SMRP12676. Positions 45-545.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
IPR006311. TAT_signal.
[Graphical view]
PfamPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PROSITEPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. False negative.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHOD_STRS0
AccessionPrimary (citable) accession number: P12676
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 28, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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