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Protein

Calpastatin

Gene

CAST

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

GO - Molecular functioni

GO - Biological processi

  • protein catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI27.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpastatin
Alternative name(s):
Calpain inhibitor
Gene namesi
Name:CAST
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476341 – 713CalpastatinAdd BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei134PhosphoserineBy similarity1
Modified residuei136PhosphothreonineBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei367PhosphoserineBy similarity1
Modified residuei369PhosphoserineBy similarity1
Modified residuei376PhosphoserineBy similarity1
Modified residuei441PhosphoserineBy similarity1
Modified residuei517PhosphoserineBy similarity1
Modified residuei528PhosphoserineBy similarity1
Modified residuei575PhosphoserineBy similarity1
Modified residuei577PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP12675.
PeptideAtlasiP12675.
PRIDEiP12675.

Expressioni

Gene expression databases

BgeeiENSSSCG00000014170.
GenevisibleiP12675. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015070.

Structurei

Secondary structure

1713
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi232 – 239Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NX0X-ray2.30C/D231-241[»]
1NX1X-ray2.00C/D231-241[»]
SMRiP12675.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati171 – 223Inhibitory domain 1Add BLAST53
Repeati307 – 359Inhibitory domain 2Add BLAST53
Repeati447 – 500Inhibitory domain 3Add BLAST54
Repeati583 – 636Inhibitory domain 4Add BLAST54

Domaini

Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFF9. Eukaryota.
ENOG4111YF0. LUCA.
HOVERGENiHBG000183.
InParanoidiP12675.
OrthoDBiEOG091G0553.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPTETKAIP VSKQLEGPHS PNKKRHKKQA VKTEPEKKSQ STKPSVVHEK
60 70 80 90 100
KTQEVKPKEH PEPKSLPTHS ADAGSKRAHK EKAVSRSNEQ PTSEKSTKPK
110 120 130 140 150
AKPQDPTPSD GKLSVTGVSA ASGKPAETKK DDKSLTSSVP AESKSSKPSG
160 170 180 190 200
KSDMDAALDD LIDTLGGPEE TEEDNTTYTG PEVLDPMSST YIEELGKREV
210 220 230 240 250
TLPPKYRELL DKKEGIPVPP PDTSKPLGPD DAIDALSLDL TCSSPTADGK
260 270 280 290 300
KTEKEKSTGE VLKAQSVGVI KSAAAPPHEK KRRVEEDTMS DQALEALSAS
310 320 330 340 350
LGSRKSEPEL DLSSIKEIDE AKAKEEKLKK CGEDDETVPP EYRLKPAMDK
360 370 380 390 400
DGKPLLPEAE EKPKPLSESE LIDELSEDFD QSKRKEKQSK PTEKTKESQA
410 420 430 440 450
TAPTPVGEAV SRTSLCCVQS APPKPATGMV PDDAVEALAG SLGKKEADPE
460 470 480 490 500
DGKPVEDKVK EKAKEEDREK LGEKEETIPP DYRLEEVKDK DGKTLPHKDP
510 520 530 540 550
KEPVLPLSED FVLDALSQDF AGPPAASSLF EDAKLSAAVS EVVSQTSAPT
560 570 580 590 600
THSAGPPPDT VSDDKKLDDA LDQLSDSLGQ RQPDPDENKP IEDKVKEKAE
610 620 630 640 650
AEHRDKLGER DDTIPPEYRH LLDKDEEGKS TKPPTKKPEA PKKPEAAQDP
660 670 680 690 700
IDALSGDFDR CPSTTETSEN TTKDKDKKTA SKSKAPKNGG KAKDSTKAKE
710
ETSKQKSDGK STS
Length:713
Mass (Da):77,124
Last modified:October 1, 1989 - v1
Checksum:iABD4E8F119CE97B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti328L → V in AAA31009 (PubMed:3780962).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20160 mRNA. Translation: AAA31012.1.
AY372988 mRNA. Translation: AAR27961.1.
M27969 mRNA. Translation: AAA31009.1.
PIRiA24627.
A28706.
UniGeneiSsc.31703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20160 mRNA. Translation: AAA31012.1.
AY372988 mRNA. Translation: AAR27961.1.
M27969 mRNA. Translation: AAA31009.1.
PIRiA24627.
A28706.
UniGeneiSsc.31703.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NX0X-ray2.30C/D231-241[»]
1NX1X-ray2.00C/D231-241[»]
SMRiP12675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015070.

Protein family/group databases

MEROPSiI27.001.

Proteomic databases

PaxDbiP12675.
PeptideAtlasiP12675.
PRIDEiP12675.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IFF9. Eukaryota.
ENOG4111YF0. LUCA.
HOVERGENiHBG000183.
InParanoidiP12675.
OrthoDBiEOG091G0553.

Gene expression databases

BgeeiENSSSCG00000014170.
GenevisibleiP12675. SS.

Family and domain databases

InterProiIPR026998. Calpastatin.
IPR001259. Prot_inh_calpain.
[Graphical view]
PANTHERiPTHR10077. PTHR10077. 1 hit.
PfamiPF00748. Calpain_inhib. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICAL_PIG
AccessioniPrimary (citable) accession number: P12675
Secondary accession number(s): Q3ZTQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.