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P12672 (RAPSN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
43 kDa receptor-associated protein of the synapse

Short name=RAPsyn
Alternative name(s):
43 kDa postsynaptic protein
Acetylcholine receptor-associated 43 kDa protein
Gene names
Name:Rapsn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junctionsynapsepostsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Note: Cytoplasmic surface of postsynaptic membranes.

Domain

A cysteine-rich region homologous to part of the regulatory domain of protein kinase C may be important in interactions of this protein with the lipid bilayer.

Post-translational modification

Ubiquitinated by the BCR(KLHL8) complex, leading to its degradation By similarity.

Sequence similarities

Belongs to the RAPsyn family.

Contains 1 RING-type zinc finger.

Contains 7 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 41241143 kDa receptor-associated protein of the synapse
PRO_0000167592

Regions

Repeat6 – 3934TPR 1
Repeat83 – 11634TPR 2
Repeat123 – 15634TPR 3
Repeat163 – 19634TPR 4
Repeat206 – 23934TPR 5
Repeat246 – 27934TPR 6
Repeat286 – 31934TPR 7
Zinc finger363 – 40341RING-type

Amino acid modifications

Modified residue1961Phosphotyrosine Potential
Modified residue4051Phosphoserine Potential
Lipidation21N-myristoyl glycine By similarity

Experimental info

Sequence conflict344 – 3452EL → DV in CAA33789. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P12672 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: EB5C656229AB0D15

FASTA41246,393
        10         20         30         40         50         60 
MGQDQTKQQI EKGLQLYQSN QTEKALQVWM KVLEKGSDLV GRFRVLGCLV TAHSEMGRYK 

        70         80         90        100        110        120 
EMLKFAVVQI DTARGLEDAD FLLESYLNLA RSNEKLCEFH KTISYCKTCL GLPGTRAGAQ 

       130        140        150        160        170        180 
LGGQVSLSMG NAFLGLSLFQ KALESFEKAL RYAHNNDDTM LECRVCCSLG SFYAQVKDYE 

       190        200        210        220        230        240 
KALFFPCKAA ELVNDYGKGW SLKYRAMSQY HMAVAYRLLG HLGSAMECCE ESMKIALQHG 

       250        260        270        280        290        300 
DRPLQALCLL CFADIHRSRG DLETAFPRYD SAMSIMTEIG NRLGQVHVLL GVAKCWMARK 

       310        320        330        340        350        360 
VQDKALDAIE KAQDLAEEVG NKLSQLKLHC LSESIYRSKG LQRELRTHVV RFHECVEETE 

       370        380        390        400        410 
LYCGLCGESI GERNSRLQAL PCSHIFHLRC LQNNGTRSCP NCRRSSMKPG FV 

« Hide

References

« Hide 'large scale' references
[1]"Expression of RNA transcripts for the postsynaptic 43 kDa protein in innervated and denervated rat skeletal muscle."
Froehner S.C.
FEBS Lett. 249:229-233(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Muscle.
[2]"Identification of the mouse muscle 43,000-dalton acetylcholine receptor-associated protein (RAPsyn) by cDNA cloning."
Frail D.E., McLaughlin L.L., Mudd J., Merlie J.P.
J. Biol. Chem. 263:15602-15607(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Muscle.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15788 mRNA. Translation: CAA33789.1.
J03962 mRNA. Translation: AAA40030.1.
AL672241 Genomic DNA. Translation: CAM15326.1.
CH466519 Genomic DNA. Translation: EDL27517.1.
BC111863 mRNA. Translation: AAI11864.1.
CCDSCCDS16422.1.
PIRA31995.
RefSeqNP_033049.2. NM_009023.3.
UniGeneMm.1272.

3D structure databases

ProteinModelPortalP12672.
SMRP12672. Positions 18-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202585. 1 interaction.
IntActP12672. 1 interaction.
MINTMINT-2983138.
STRING10090.ENSMUSP00000054150.

PTM databases

PhosphoSiteP12672.

Proteomic databases

PRIDEP12672.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050323; ENSMUSP00000054150; ENSMUSG00000002104.
GeneID19400.
KEGGmmu:19400.
UCSCuc008kud.2. mouse.

Organism-specific databases

CTD5913.
MGIMGI:99422. Rapsn.

Phylogenomic databases

eggNOGCOG0457.
GeneTreeENSGT00390000016785.
HOGENOMHOG000231395.
HOVERGENHBG061484.
InParanoidQ2M2N6.
OMAEEMELYC.
TreeFamTF328344.

Gene expression databases

BgeeP12672.
CleanExMM_RAPSN.
GenevestigatorP12672.

Family and domain databases

Gene3D1.25.40.10. 4 hits.
3.30.40.10. 1 hit.
InterProIPR001237. Postsynaptic.
IPR018293. Postsynaptic_CS.
IPR019568. Rapsyn_myristoylation/link_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF10579. Rapsyn_N. 1 hit.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
PRINTSPR00217. POSTSYNAPTIC.
SMARTSM00184. RING. 1 hit.
SM00028. TPR. 6 hits.
[Graphical view]
PROSITEPS00405. 43_KD_POSTSYNAPTIC. 1 hit.
PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296509.
PROP12672.
SOURCESearch...

Entry information

Entry nameRAPSN_MOUSE
AccessionPrimary (citable) accession number: P12672
Secondary accession number(s): Q2M2N6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot