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P12672

- RAPSN_MOUSE

UniProt

P12672 - RAPSN_MOUSE

Protein

43 kDa receptor-associated protein of the synapse

Gene

Rapsn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 4 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri363 – 40341RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. acetylcholine receptor binding Source: MGI
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. positive regulation of neuron apoptotic process Source: MGI
    2. synaptic transmission, cholinergic Source: Ensembl

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    43 kDa receptor-associated protein of the synapse
    Short name:
    RAPsyn
    Alternative name(s):
    43 kDa postsynaptic protein
    Acetylcholine receptor-associated 43 kDa protein
    Gene namesi
    Name:Rapsn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:99422. Rapsn.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoskeleton Source: UniProtKB-SubCell
    3. Golgi apparatus Source: MGI
    4. neuromuscular junction Source: MGI
    5. plasma membrane Source: MGI
    6. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 41241143 kDa receptor-associated protein of the synapsePRO_0000167592Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Modified residuei196 – 1961PhosphotyrosineSequence Analysis
    Modified residuei405 – 4051PhosphoserineSequence Analysis

    Post-translational modificationi

    Ubiquitinated by the BCR(KLHL8) complex, leading to its degradation.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP12672.

    PTM databases

    PhosphoSiteiP12672.

    Expressioni

    Gene expression databases

    BgeeiP12672.
    CleanExiMM_RAPSN.
    GenevestigatoriP12672.

    Interactioni

    Protein-protein interaction databases

    BioGridi202585. 1 interaction.
    IntActiP12672. 1 interaction.
    MINTiMINT-2983138.
    STRINGi10090.ENSMUSP00000054150.

    Structurei

    3D structure databases

    ProteinModelPortaliP12672.
    SMRiP12672. Positions 18-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati6 – 3934TPR 1Add
    BLAST
    Repeati83 – 11634TPR 2Add
    BLAST
    Repeati123 – 15634TPR 3Add
    BLAST
    Repeati163 – 19634TPR 4Add
    BLAST
    Repeati206 – 23934TPR 5Add
    BLAST
    Repeati246 – 27934TPR 6Add
    BLAST
    Repeati286 – 31934TPR 7Add
    BLAST

    Domaini

    A cysteine-rich region homologous to part of the regulatory domain of protein kinase C may be important in interactions of this protein with the lipid bilayer.

    Sequence similaritiesi

    Belongs to the RAPsyn family.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 7 TPR repeats.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri363 – 40341RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, TPR repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0457.
    GeneTreeiENSGT00390000016785.
    HOGENOMiHOG000231395.
    HOVERGENiHBG061484.
    InParanoidiQ2M2N6.
    OMAiEEMELYC.
    TreeFamiTF328344.

    Family and domain databases

    Gene3Di1.25.40.10. 4 hits.
    3.30.40.10. 1 hit.
    InterProiIPR001237. Postsynaptic.
    IPR018293. Postsynaptic_CS.
    IPR019568. Rapsyn_myristoylation/link_N.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF10579. Rapsyn_N. 1 hit.
    PF00515. TPR_1. 1 hit.
    PF13181. TPR_8. 1 hit.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view]
    PRINTSiPR00217. POSTSYNAPTIC.
    SMARTiSM00184. RING. 1 hit.
    SM00028. TPR. 6 hits.
    [Graphical view]
    PROSITEiPS00405. 43_KD_POSTSYNAPTIC. 1 hit.
    PS50005. TPR. 5 hits.
    PS50293. TPR_REGION. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12672-1 [UniParc]FASTAAdd to Basket

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    MGQDQTKQQI EKGLQLYQSN QTEKALQVWM KVLEKGSDLV GRFRVLGCLV    50
    TAHSEMGRYK EMLKFAVVQI DTARGLEDAD FLLESYLNLA RSNEKLCEFH 100
    KTISYCKTCL GLPGTRAGAQ LGGQVSLSMG NAFLGLSLFQ KALESFEKAL 150
    RYAHNNDDTM LECRVCCSLG SFYAQVKDYE KALFFPCKAA ELVNDYGKGW 200
    SLKYRAMSQY HMAVAYRLLG HLGSAMECCE ESMKIALQHG DRPLQALCLL 250
    CFADIHRSRG DLETAFPRYD SAMSIMTEIG NRLGQVHVLL GVAKCWMARK 300
    VQDKALDAIE KAQDLAEEVG NKLSQLKLHC LSESIYRSKG LQRELRTHVV 350
    RFHECVEETE LYCGLCGESI GERNSRLQAL PCSHIFHLRC LQNNGTRSCP 400
    NCRRSSMKPG FV 412
    Length:412
    Mass (Da):46,393
    Last modified:July 27, 2011 - v4
    Checksum:iEB5C656229AB0D15
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti344 – 3452EL → DV in CAA33789. (PubMed:2737281)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15788 mRNA. Translation: CAA33789.1.
    J03962 mRNA. Translation: AAA40030.1.
    AL672241 Genomic DNA. Translation: CAM15326.1.
    CH466519 Genomic DNA. Translation: EDL27517.1.
    BC111863 mRNA. Translation: AAI11864.1.
    CCDSiCCDS16422.1.
    PIRiA31995.
    RefSeqiNP_033049.2. NM_009023.3.
    UniGeneiMm.1272.

    Genome annotation databases

    EnsembliENSMUST00000050323; ENSMUSP00000054150; ENSMUSG00000002104.
    GeneIDi19400.
    KEGGimmu:19400.
    UCSCiuc008kud.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15788 mRNA. Translation: CAA33789.1 .
    J03962 mRNA. Translation: AAA40030.1 .
    AL672241 Genomic DNA. Translation: CAM15326.1 .
    CH466519 Genomic DNA. Translation: EDL27517.1 .
    BC111863 mRNA. Translation: AAI11864.1 .
    CCDSi CCDS16422.1.
    PIRi A31995.
    RefSeqi NP_033049.2. NM_009023.3.
    UniGenei Mm.1272.

    3D structure databases

    ProteinModelPortali P12672.
    SMRi P12672. Positions 18-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202585. 1 interaction.
    IntActi P12672. 1 interaction.
    MINTi MINT-2983138.
    STRINGi 10090.ENSMUSP00000054150.

    PTM databases

    PhosphoSitei P12672.

    Proteomic databases

    PRIDEi P12672.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050323 ; ENSMUSP00000054150 ; ENSMUSG00000002104 .
    GeneIDi 19400.
    KEGGi mmu:19400.
    UCSCi uc008kud.2. mouse.

    Organism-specific databases

    CTDi 5913.
    MGIi MGI:99422. Rapsn.

    Phylogenomic databases

    eggNOGi COG0457.
    GeneTreei ENSGT00390000016785.
    HOGENOMi HOG000231395.
    HOVERGENi HBG061484.
    InParanoidi Q2M2N6.
    OMAi EEMELYC.
    TreeFami TF328344.

    Miscellaneous databases

    NextBioi 296509.
    PROi P12672.
    SOURCEi Search...

    Gene expression databases

    Bgeei P12672.
    CleanExi MM_RAPSN.
    Genevestigatori P12672.

    Family and domain databases

    Gene3Di 1.25.40.10. 4 hits.
    3.30.40.10. 1 hit.
    InterProi IPR001237. Postsynaptic.
    IPR018293. Postsynaptic_CS.
    IPR019568. Rapsyn_myristoylation/link_N.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF10579. Rapsyn_N. 1 hit.
    PF00515. TPR_1. 1 hit.
    PF13181. TPR_8. 1 hit.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00217. POSTSYNAPTIC.
    SMARTi SM00184. RING. 1 hit.
    SM00028. TPR. 6 hits.
    [Graphical view ]
    PROSITEi PS00405. 43_KD_POSTSYNAPTIC. 1 hit.
    PS50005. TPR. 5 hits.
    PS50293. TPR_REGION. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of RNA transcripts for the postsynaptic 43 kDa protein in innervated and denervated rat skeletal muscle."
      Froehner S.C.
      FEBS Lett. 249:229-233(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Muscle.
    2. "Identification of the mouse muscle 43,000-dalton acetylcholine receptor-associated protein (RAPsyn) by cDNA cloning."
      Frail D.E., McLaughlin L.L., Mudd J., Merlie J.P.
      J. Biol. Chem. 263:15602-15607(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Muscle.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiRAPSN_MOUSE
    AccessioniPrimary (citable) accession number: P12672
    Secondary accession number(s): Q2M2N6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 138 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3