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Protein

43 kDa receptor-associated protein of the synapse

Gene

Rapsn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri363 – 40341RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • acetylcholine receptor binding Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • positive regulation of neuron apoptotic process Source: MGI
  • synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
43 kDa receptor-associated protein of the synapse
Short name:
RAPsyn
Alternative name(s):
43 kDa postsynaptic protein
Acetylcholine receptor-associated 43 kDa protein
Gene namesi
Name:Rapsn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99422. Rapsn.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • Golgi apparatus Source: MGI
  • membrane Source: MGI
  • neuromuscular junction Source: MGI
  • plasma membrane Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 41241143 kDa receptor-associated protein of the synapsePRO_0000167592Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei196 – 1961PhosphotyrosineSequence Analysis
Modified residuei405 – 4051PhosphoserineSequence Analysis

Post-translational modificationi

Ubiquitinated by the BCR(KLHL8) complex, leading to its degradation.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP12672.

PTM databases

PhosphoSiteiP12672.

Expressioni

Gene expression databases

BgeeiP12672.
CleanExiMM_RAPSN.
ExpressionAtlasiP12672. baseline and differential.
GenevisibleiP12672. MM.

Interactioni

Protein-protein interaction databases

BioGridi202585. 1 interaction.
IntActiP12672. 1 interaction.
MINTiMINT-2983138.
STRINGi10090.ENSMUSP00000054150.

Structurei

3D structure databases

ProteinModelPortaliP12672.
SMRiP12672. Positions 18-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 3934TPR 1Add
BLAST
Repeati83 – 11634TPR 2Add
BLAST
Repeati123 – 15634TPR 3Add
BLAST
Repeati163 – 19634TPR 4Add
BLAST
Repeati206 – 23934TPR 5Add
BLAST
Repeati246 – 27934TPR 6Add
BLAST
Repeati286 – 31934TPR 7Add
BLAST

Domaini

A cysteine-rich region homologous to part of the regulatory domain of protein kinase C may be important in interactions of this protein with the lipid bilayer.

Sequence similaritiesi

Belongs to the RAPsyn family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 7 TPR repeats.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri363 – 40341RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, TPR repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00390000016785.
HOGENOMiHOG000231395.
HOVERGENiHBG061484.
InParanoidiP12672.
OMAiCCSLGNF.
TreeFamiTF328344.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
3.30.40.10. 1 hit.
InterProiIPR001237. Postsynaptic.
IPR018293. Postsynaptic_CS.
IPR019568. Rapsyn_myristoylation/link_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF10579. Rapsyn_N. 1 hit.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
PRINTSiPR00217. POSTSYNAPTIC.
SMARTiSM00184. RING. 1 hit.
SM00028. TPR. 6 hits.
[Graphical view]
PROSITEiPS00405. 43_KD_POSTSYNAPTIC. 1 hit.
PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12672-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQDQTKQQI EKGLQLYQSN QTEKALQVWM KVLEKGSDLV GRFRVLGCLV
60 70 80 90 100
TAHSEMGRYK EMLKFAVVQI DTARGLEDAD FLLESYLNLA RSNEKLCEFH
110 120 130 140 150
KTISYCKTCL GLPGTRAGAQ LGGQVSLSMG NAFLGLSLFQ KALESFEKAL
160 170 180 190 200
RYAHNNDDTM LECRVCCSLG SFYAQVKDYE KALFFPCKAA ELVNDYGKGW
210 220 230 240 250
SLKYRAMSQY HMAVAYRLLG HLGSAMECCE ESMKIALQHG DRPLQALCLL
260 270 280 290 300
CFADIHRSRG DLETAFPRYD SAMSIMTEIG NRLGQVHVLL GVAKCWMARK
310 320 330 340 350
VQDKALDAIE KAQDLAEEVG NKLSQLKLHC LSESIYRSKG LQRELRTHVV
360 370 380 390 400
RFHECVEETE LYCGLCGESI GERNSRLQAL PCSHIFHLRC LQNNGTRSCP
410
NCRRSSMKPG FV
Length:412
Mass (Da):46,393
Last modified:July 27, 2011 - v4
Checksum:iEB5C656229AB0D15
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti344 – 3452EL → DV in CAA33789 (PubMed:2737281).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15788 mRNA. Translation: CAA33789.1.
J03962 mRNA. Translation: AAA40030.1.
AL672241 Genomic DNA. Translation: CAM15326.1.
CH466519 Genomic DNA. Translation: EDL27517.1.
BC111863 mRNA. Translation: AAI11864.1.
CCDSiCCDS16422.1.
PIRiA31995.
RefSeqiNP_033049.2. NM_009023.3.
UniGeneiMm.1272.

Genome annotation databases

EnsembliENSMUST00000050323; ENSMUSP00000054150; ENSMUSG00000002104.
GeneIDi19400.
KEGGimmu:19400.
UCSCiuc008kud.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15788 mRNA. Translation: CAA33789.1.
J03962 mRNA. Translation: AAA40030.1.
AL672241 Genomic DNA. Translation: CAM15326.1.
CH466519 Genomic DNA. Translation: EDL27517.1.
BC111863 mRNA. Translation: AAI11864.1.
CCDSiCCDS16422.1.
PIRiA31995.
RefSeqiNP_033049.2. NM_009023.3.
UniGeneiMm.1272.

3D structure databases

ProteinModelPortaliP12672.
SMRiP12672. Positions 18-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202585. 1 interaction.
IntActiP12672. 1 interaction.
MINTiMINT-2983138.
STRINGi10090.ENSMUSP00000054150.

PTM databases

PhosphoSiteiP12672.

Proteomic databases

PRIDEiP12672.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050323; ENSMUSP00000054150; ENSMUSG00000002104.
GeneIDi19400.
KEGGimmu:19400.
UCSCiuc008kud.2. mouse.

Organism-specific databases

CTDi5913.
MGIiMGI:99422. Rapsn.

Phylogenomic databases

eggNOGiCOG0457.
GeneTreeiENSGT00390000016785.
HOGENOMiHOG000231395.
HOVERGENiHBG061484.
InParanoidiP12672.
OMAiCCSLGNF.
TreeFamiTF328344.

Miscellaneous databases

NextBioi296509.
PROiP12672.
SOURCEiSearch...

Gene expression databases

BgeeiP12672.
CleanExiMM_RAPSN.
ExpressionAtlasiP12672. baseline and differential.
GenevisibleiP12672. MM.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
3.30.40.10. 1 hit.
InterProiIPR001237. Postsynaptic.
IPR018293. Postsynaptic_CS.
IPR019568. Rapsyn_myristoylation/link_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF10579. Rapsyn_N. 1 hit.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
PRINTSiPR00217. POSTSYNAPTIC.
SMARTiSM00184. RING. 1 hit.
SM00028. TPR. 6 hits.
[Graphical view]
PROSITEiPS00405. 43_KD_POSTSYNAPTIC. 1 hit.
PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of RNA transcripts for the postsynaptic 43 kDa protein in innervated and denervated rat skeletal muscle."
    Froehner S.C.
    FEBS Lett. 249:229-233(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Muscle.
  2. "Identification of the mouse muscle 43,000-dalton acetylcholine receptor-associated protein (RAPsyn) by cDNA cloning."
    Frail D.E., McLaughlin L.L., Mudd J., Merlie J.P.
    J. Biol. Chem. 263:15602-15607(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Muscle.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiRAPSN_MOUSE
AccessioniPrimary (citable) accession number: P12672
Secondary accession number(s): Q2M2N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 145 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.