43 kDa receptor-associated protein of the synapse

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P12672 (RAPSN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
43 kDa receptor-associated protein of the synapse
Short name=RAPsyn

Alternative name(s):
43 kDa postsynaptic protein
Acetylcholine receptor-associated 43 kDa protein

Gene names

Name:

Rapsn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thought to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse › postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Note: Cytoplasmic surface of postsynaptic membranes.
Domain	A cysteine-rich region homologous to part of the regulatory domain of protein kinase C may be important in interactions of this protein with the lipid bilayer.
Sequence similarities	Belongs to the RAPsyn family. Contains 1 RING-type zinc finger. Contains 7 TPR repeats.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Postsynaptic cell membrane Synapse
Domain	Repeat TPR repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 11312299. Source: MGI synaptic transmission, cholinergic Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 12388596. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell neuromuscular junction Inferred from direct assay PubMed 17119023 PubMed 8416841. Source: MGI plasma membrane Inferred from direct assay PubMed 12388596. Source: MGI postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 412

412

43 kDa receptor-associated protein of the synapse

PRO_0000167592

Regions

Repeat

6 – 39

TPR 1

Repeat

83 – 116

TPR 2

Repeat

123 – 156

TPR 3

Repeat

163 – 196

TPR 4

Repeat

206 – 239

TPR 5

Repeat

246 – 279

TPR 6

Repeat

286 – 319

TPR 7

Zinc finger

363 – 403

RING-type

Amino acid modifications

Modified residue

196

Phosphotyrosine Potential

Modified residue

405

Phosphoserine Potential

Lipidation

N-myristoyl glycine By similarity

Experimental info

Sequence conflict

344 – 345

EL → DV in CAA33789. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P12672 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: EB5C656229AB0D15
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FASTA

412

46,393

        10         20         30         40         50         60 
MGQDQTKQQI EKGLQLYQSN QTEKALQVWM KVLEKGSDLV GRFRVLGCLV TAHSEMGRYK 

        70         80         90        100        110        120 
EMLKFAVVQI DTARGLEDAD FLLESYLNLA RSNEKLCEFH KTISYCKTCL GLPGTRAGAQ 

       130        140        150        160        170        180 
LGGQVSLSMG NAFLGLSLFQ KALESFEKAL RYAHNNDDTM LECRVCCSLG SFYAQVKDYE 

       190        200        210        220        230        240 
KALFFPCKAA ELVNDYGKGW SLKYRAMSQY HMAVAYRLLG HLGSAMECCE ESMKIALQHG 

       250        260        270        280        290        300 
DRPLQALCLL CFADIHRSRG DLETAFPRYD SAMSIMTEIG NRLGQVHVLL GVAKCWMARK 

       310        320        330        340        350        360 
VQDKALDAIE KAQDLAEEVG NKLSQLKLHC LSESIYRSKG LQRELRTHVV RFHECVEETE 

       370        380        390        400        410 
LYCGLCGESI GERNSRLQAL PCSHIFHLRC LQNNGTRSCP NCRRSSMKPG FV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of RNA transcripts for the postsynaptic 43 kDa protein in innervated and denervated rat skeletal muscle." Froehner S.C. FEBS Lett. 249:229-233(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[2]	"Identification of the mouse muscle 43,000-dalton acetylcholine receptor-associated protein (RAPsyn) by cDNA cloning." Frail D.E., McLaughlin L.L., Mudd J., Merlie J.P. J. Biol. Chem. 263:15602-15607(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15788 mRNA. Translation: CAA33789.1. J03962 mRNA. Translation: AAA40030.1. AL672241 Genomic DNA. Translation: CAM15326.1. CH466519 Genomic DNA. Translation: EDL27517.1. BC111863 mRNA. Translation: AAI11864.1.
IPI	IPI00222353.
PIR	A31995.
RefSeq	NP_033049.2. NM_009023.3.
UniGene	Mm.1272.
3D structure databases
ProteinModelPortal	P12672.
SMR	P12672. Positions 4-326.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2983138.
STRING	10090.ENSMUSP00000054150.
PTM databases
PhosphoSite	P12672.
Proteomic databases
PRIDE	P12672.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050323; ENSMUSP00000054150; ENSMUSG00000002104.
GeneID	19400.
KEGG	mmu:19400.
UCSC	uc008kud.2. mouse.
Organism-specific databases
CTD	5913.
MGI	MGI:99422. Rapsn.
Phylogenomic databases
eggNOG	COG0457.
GeneTree	ENSGT00390000016785.
HOGENOM	HOG000231395.
HOVERGEN	HBG061484.
InParanoid	Q2M2N6.
OMA	EEMELYC.
OrthoDB	EOG405S12.
Gene expression databases
ArrayExpress	P12672.
Bgee	P12672.
CleanEx	MM_RAPSN.
Genevestigator	P12672.
GermOnline	ENSMUSG00000002104. Mus musculus.
Family and domain databases
Gene3D	1.25.40.10. 4 hits. 3.30.40.10. 1 hit.
InterPro	IPR001237. Postsynaptic. IPR018293. Postsynaptic_CS. IPR019568. Rapsyn_myristoylation/link_N. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF10579. Rapsyn_N. 1 hit. PF00515. TPR_1. 1 hit. PF13181. TPR_8. 1 hit. PF13639. zf-RING_2. 1 hit. [Graphical view]
PRINTS	PR00217. POSTSYNAPTIC.
SMART	SM00184. RING. 1 hit. SM00028. TPR. 6 hits. [Graphical view]
PROSITE	PS00405. 43_KD_POSTSYNAPTIC. 1 hit. PS50005. TPR. 5 hits. PS50293. TPR_REGION. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296509.
SOURCE	Search...

Entry information

Entry name

RAPSN_MOUSE

Accession

Primary (citable) accession number: P12672
Secondary accession number(s): Q2M2N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 127 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents