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Protein

Protein NP24

Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has antifungal activity against P.betae and F.dahliae. May be involved in disease resistance in tomatoes and/or have a possible role in fruit development and ripening.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide, Pathogenesis-related protein

Keywords - Biological processi

Plant defense, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NP24
Alternative name(s):
Pathogenesis-related protein PR P23
Salt-induced protein
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 8

Subcellular locationi

  • Cytoplasm

  • Note: Or soluble fractions of cytoplasmic organelles, except mitochondria and plastids.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei9063. Sola l TLP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 247226Protein NP24PRO_0000034044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 225PROSITE-ProRule annotation
Disulfide bondi72 ↔ 82PROSITE-ProRule annotation
Disulfide bondi87 ↔ 93PROSITE-ProRule annotation
Disulfide bondi141 ↔ 213PROSITE-ProRule annotation
Disulfide bondi146 ↔ 196PROSITE-ProRule annotation
Disulfide bondi154 ↔ 164PROSITE-ProRule annotation
Disulfide bondi168 ↔ 177PROSITE-ProRule annotation
Disulfide bondi178 ↔ 183PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP12670.

Expressioni

Tissue specificityi

Highest levels of both isoforms found in the outer pericarp, with smaller amounts in the inner pericarp.1 Publication

Developmental stagei

NP24 I is low in green tomatoes and it increased substantially during ripening, with the largest increase occurring between the pink and red stages. NP24 II is relatively high in green tomatoes and it increased somewhat as the fruit turned pink, but not during further ripening.1 Publication

Inductioni

By salt stress or by viroids.

Interactioni

Protein-protein interaction databases

STRINGi4081.Solyc08g080640.1.1.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 286Combined sources
Beta strandi30 – 323Combined sources
Beta strandi34 – 396Combined sources
Turni40 – 423Combined sources
Beta strandi43 – 475Combined sources
Beta strandi52 – 565Combined sources
Beta strandi63 – 719Combined sources
Beta strandi73 – 753Combined sources
Turni76 – 783Combined sources
Beta strandi79 – 813Combined sources
Beta strandi83 – 853Combined sources
Beta strandi88 – 925Combined sources
Beta strandi103 – 1097Combined sources
Turni110 – 1134Combined sources
Beta strandi114 – 1207Combined sources
Beta strandi125 – 1273Combined sources
Beta strandi129 – 1357Combined sources
Beta strandi144 – 1463Combined sources
Helixi150 – 1534Combined sources
Turni156 – 1583Combined sources
Helixi167 – 1704Combined sources
Helixi174 – 1774Combined sources
Helixi189 – 1957Combined sources
Beta strandi199 – 2024Combined sources
Turni207 – 2093Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi220 – 2256Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I0WX-ray2.50A22-228[»]
ProteinModelPortaliP12670.
SMRiP12670. Positions 22-228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12670.

Family & Domainsi

Sequence similaritiesi

Belongs to the thaumatin family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIHC. Eukaryota.
ENOG410YD4X. LUCA.
InParanoidiP12670.
OMAiNGECPRA.

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYLTSSFVL FFLLCVTYTY AATIEVRNNC PYTVWAASTP IGGGRRLNRG
60 70 80 90 100
QTWVINAPRG TKMARIWGRT GCNFNAAGRG TCQTGDCGGV LQCTGWGKPP
110 120 130 140 150
NTLAEYALDQ FSNLDFWDIS LVDGFNIPMT FAPTKPSGGK CHAIHCTANI
160 170 180 190 200
NGECPRALKV PGGCNNPCTT FGGQQYCCTQ GPCGPTELSK FFKKRCPDAY
210 220 230 240
SYPQDDPTST FTCPGGSTNY RVVFCPNGVA DPNFPLEMPA STDEVAK
Length:247
Mass (Da):26,646
Last modified:July 15, 1999 - v2
Checksum:i942E56E2531031B4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241I → F in strain: cv. Rutgers and NP24 II. 1 Publication
Natural varianti38 – 381S → F in NP24 II. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093743 Genomic DNA. Translation: AAC64171.1.
M21346 mRNA. Translation: AAA34175.1.
PIRiS07406.
S16264.
RefSeqiNP_001292922.1. NM_001305993.1.
UniGeneiLes.19348.

Genome annotation databases

EnsemblPlantsiSolyc08g080640.1.1; Solyc08g080640.1.1; Solyc08g080640.1.
GeneIDi543979.
GrameneiSolyc08g080640.1.1; Solyc08g080640.1.1; Solyc08g080640.1.
KEGGisly:543979.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093743 Genomic DNA. Translation: AAC64171.1.
M21346 mRNA. Translation: AAA34175.1.
PIRiS07406.
S16264.
RefSeqiNP_001292922.1. NM_001305993.1.
UniGeneiLes.19348.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I0WX-ray2.50A22-228[»]
ProteinModelPortaliP12670.
SMRiP12670. Positions 22-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc08g080640.1.1.

Protein family/group databases

Allergomei9063. Sola l TLP.

Proteomic databases

PaxDbiP12670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc08g080640.1.1; Solyc08g080640.1.1; Solyc08g080640.1.
GeneIDi543979.
GrameneiSolyc08g080640.1.1; Solyc08g080640.1.1; Solyc08g080640.1.
KEGGisly:543979.

Phylogenomic databases

eggNOGiENOG410IIHC. Eukaryota.
ENOG410YD4X. LUCA.
InParanoidiP12670.
OMAiNGECPRA.

Miscellaneous databases

EvolutionaryTraceiP12670.

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rapid transcript accumulation of pathogenesis-related genes during an incompatible interaction in bacterial speck disease resistant tomato plants."
    Martin G.B., Jia Y.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Ailsa Craig.
  2. "Isolation and characterization of a tomato cDNA clone which codes for a salt-induced protein."
    King G.J., Turner V.A., Hussey C.E. Jr., Wurtele E.S., Lee S.M.
    Plant Mol. Biol. 10:401-412(1988)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE OF 9-247, PARTIAL PROTEIN SEQUENCE.
    Strain: cv. VFNT Cherry.
  3. "Identification of the viroid-induced tomato pathogenesis-related (PR) protein P23 as the thaumatin-like tomato protein NP24 associated with osmotic stress."
    Rodrigo I., Vera P., Frank R., Conejero V.
    Plant Mol. Biol. 16:931-934(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Rutgers.
    Tissue: Leaf.
  4. "Two isoforms of NP24: a thaumatin-like protein in tomato fruit."
    Pressey R.
    Phytochemistry 44:1241-1245(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-46, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANTS NP24 II PHE-24 AND PHE-38.
    Strain: cv. Better Boy.
    Tissue: Pericarp.

Entry informationi

Entry nameiNP24_SOLLC
AccessioniPrimary (citable) accession number: P12670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1999
Last modified: February 17, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.