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Protein

Calbindin

Gene

Calb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi24 – 35121Add
BLAST
Calcium bindingi111 – 122122Add
BLAST
Calcium bindingi155 – 166123Add
BLAST
Calcium bindingi199 – 210124Add
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular response to organic substance Source: MGI
  • locomotory behavior Source: MGI
  • long-term memory Source: MGI
  • metanephric collecting duct development Source: UniProtKB
  • metanephric connecting tubule development Source: UniProtKB
  • metanephric distal convoluted tubule development Source: UniProtKB
  • metanephric part of ureteric bud development Source: UniProtKB
  • regulation of cytosolic calcium ion concentration Source: Ensembl
  • regulation of synaptic plasticity Source: Ensembl
  • retina development in camera-type eye Source: UniProtKB
  • retina layer formation Source: UniProtKB
  • short-term memory Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Vitamin D

Names & Taxonomyi

Protein namesi
Recommended name:
Calbindin
Alternative name(s):
Calbindin D28
D-28K
PCD-29
Spot 35 protein
Vitamin D-dependent calcium-binding protein, avian-type
Gene namesi
Name:Calb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:88248. Calb1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: BHF-UCL
  • cell body Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • dendrite Source: MGI
  • extracellular exosome Source: MGI
  • intracellular Source: MGI
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
  • nucleus Source: UniProtKB
  • synapse Source: MGI
  • terminal bouton Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 261260CalbindinPRO_0000073473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP12658.
PaxDbiP12658.
PRIDEiP12658.

PTM databases

iPTMnetiP12658.
PhosphoSiteiP12658.

Expressioni

Gene expression databases

BgeeiP12658.
CleanExiMM_CALB1.
GenevisibleiP12658. MM.

Interactioni

Subunit structurei

Interacts with RANBP9.By similarity

Protein-protein interaction databases

IntActiP12658. 1 interaction.
MINTiMINT-4996422.
STRINGi10090.ENSMUSP00000029876.

Structurei

3D structure databases

ProteinModelPortaliP12658.
SMRiP12658. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 4636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini53 – 8836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 13336EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini142 – 17736EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini186 – 22136EF-hand 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 76Interaction with RANBP9By similarity

Domaini

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Sequence similaritiesi

Belongs to the calbindin family.Curated
Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP12658.
KOiK14757.
OMAiFFRCQQL.
OrthoDBiEOG71G9VB.
PhylomeDBiP12658.
TreeFamiTF325083.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELLQARKKA
60 70 80 90 100
GLELSPEMKS FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC
110 120 130 140 150
EEFMKTWRKY DTDHSGFIET EELKNFLKDL LEKANKTVDD TKLAEYTDLM
160 170 180 190 200
LKLFDSNNDG KLELTEMARL LPVQENFLLK FQGIKMCGKE FNKAFELYDQ
210 220 230 240 250
DGNGYIDENE LDALLKDLCE KNKQELDINN ITTYKKNIMA LSDGGKLYRT
260
DLALILSAGD N
Length:261
Mass (Da):29,994
Last modified:January 23, 2007 - v2
Checksum:iBCC600FD1A86AF3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21531 mRNA. Translation: AAA39898.1.
D26352 Genomic DNA. Translation: BAA05386.1.
AK002635 mRNA. Translation: BAB22249.1.
AK005081 mRNA. Translation: BAB23805.1.
AK005243 mRNA. Translation: BAB23900.1.
AK028050 mRNA. Translation: BAC25721.1.
AK048517 mRNA. Translation: BAC33354.1.
AK077499 mRNA. Translation: BAC36831.1.
BC016421 mRNA. Translation: AAH16421.1.
M23663 mRNA. Translation: AAA53198.1.
CCDSiCCDS17984.1.
PIRiA34955.
RefSeqiNP_033918.1. NM_009788.4.
UniGeneiMm.277665.

Genome annotation databases

EnsembliENSMUST00000029876; ENSMUSP00000029876; ENSMUSG00000028222.
GeneIDi12307.
KEGGimmu:12307.
UCSCiuc008sbl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21531 mRNA. Translation: AAA39898.1.
D26352 Genomic DNA. Translation: BAA05386.1.
AK002635 mRNA. Translation: BAB22249.1.
AK005081 mRNA. Translation: BAB23805.1.
AK005243 mRNA. Translation: BAB23900.1.
AK028050 mRNA. Translation: BAC25721.1.
AK048517 mRNA. Translation: BAC33354.1.
AK077499 mRNA. Translation: BAC36831.1.
BC016421 mRNA. Translation: AAH16421.1.
M23663 mRNA. Translation: AAA53198.1.
CCDSiCCDS17984.1.
PIRiA34955.
RefSeqiNP_033918.1. NM_009788.4.
UniGeneiMm.277665.

3D structure databases

ProteinModelPortaliP12658.
SMRiP12658. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12658. 1 interaction.
MINTiMINT-4996422.
STRINGi10090.ENSMUSP00000029876.

PTM databases

iPTMnetiP12658.
PhosphoSiteiP12658.

Proteomic databases

MaxQBiP12658.
PaxDbiP12658.
PRIDEiP12658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029876; ENSMUSP00000029876; ENSMUSG00000028222.
GeneIDi12307.
KEGGimmu:12307.
UCSCiuc008sbl.1. mouse.

Organism-specific databases

CTDi793.
MGIiMGI:88248. Calb1.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP12658.
KOiK14757.
OMAiFFRCQQL.
OrthoDBiEOG71G9VB.
PhylomeDBiP12658.
TreeFamiTF325083.

Miscellaneous databases

PROiP12658.
SOURCEiSearch...

Gene expression databases

BgeeiP12658.
CleanExiMM_CALB1.
GenevisibleiP12658. MM.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons."
    Nordquist D.T., Kozak C.A., Orr H.T.
    J. Neurosci. 8:4780-4789(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Organization and expression of the mouse spot35/calbindin-D28k gene: identification of the vitamin D-responsive promoter region."
    Takeda T., Arakawa M., Kuwano R.
    Biochem. Biophys. Res. Commun. 204:889-897(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/cJ.
    Tissue: Liver.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Head and Kidney.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  5. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 35-47; 170-180; 222-235 AND 237-246, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  6. "Molecular cloning of mammalian 28,000 Mr vitamin D-dependent calcium binding protein (calbindin-D28K): expression of calbindin-D28K RNAs in rodent brain and kidney."
    Wood T.L., Kobayashi Y., Frantz G., Varghese S., Christakos S., Tobin A.J.
    DNA 7:585-593(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 102-261.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney and Liver.

Entry informationi

Entry nameiCALB1_MOUSE
AccessioniPrimary (citable) accession number: P12658
Secondary accession number(s): Q545N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.