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P12657 (ACM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M1
Gene names
Name:Chrm1
Synonyms:Chrm-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subunit structure

Interacts with GPRASP2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcognition

Inferred from electronic annotation. Source: InterPro

neuromuscular synaptic transmission

Inferred from electronic annotation. Source: Ensembl

positive regulation of intracellular protein transport

Inferred from electronic annotation. Source: Ensembl

positive regulation of ion transport

Inferred from sequence orthology PubMed 14657398. Source: MGI

regulation of locomotion

Inferred from mutant phenotype PubMed 11752469. Source: MGI

saliva secretion

Inferred from electronic annotation. Source: InterPro

   Cellular_componentasymmetric synapse

Inferred from electronic annotation. Source: Ensembl

axon terminus

Inferred from electronic annotation. Source: Ensembl

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled acetylcholine receptor activity

Inferred from electronic annotation. Source: Ensembl

drug binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Muscarinic acetylcholine receptor M1
PRO_0000069017

Regions

Topological domain1 – 2424Extracellular Potential
Transmembrane25 – 4723Helical; Name=1; By similarity
Topological domain48 – 6114Cytoplasmic Potential
Transmembrane62 – 8221Helical; Name=2; By similarity
Topological domain83 – 9917Extracellular Potential
Transmembrane100 – 12122Helical; Name=3; By similarity
Topological domain122 – 14120Cytoplasmic Potential
Transmembrane142 – 16423Helical; Name=4; By similarity
Topological domain165 – 18622Extracellular Potential
Transmembrane187 – 20923Helical; Name=5; By similarity
Topological domain210 – 366157Cytoplasmic Potential
Transmembrane367 – 38721Helical; Name=6; By similarity
Topological domain388 – 40114Extracellular Potential
Transmembrane402 – 42120Helical; Name=7; By similarity
Topological domain422 – 46039Cytoplasmic Potential

Amino acid modifications

Modified residue4511Phosphoserine Potential
Modified residue4551Phosphothreonine Potential
Modified residue4571Phosphoserine Potential
Glycosylation21N-linked (GlcNAc...) Probable
Glycosylation121N-linked (GlcNAc...) Probable
Disulfide bond98 ↔ 178 By similarity

Experimental info

Sequence conflict301I → S in AAA37158. Ref.1
Sequence conflict501F → I in AAA37158. Ref.1
Sequence conflict4201L → S in AAA37158. Ref.1
Sequence conflict4281T → H in AAA37158. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P12657 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 2F6A93DE8FF4F325

FASTA46051,379
        10         20         30         40         50         60 
MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 

        70         80         90        100        110        120 
NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 

       130        140        150        160        170        180 
FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 

       190        200        210        220        230        240 
QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 

       250        260        270        280        290        300 
SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 

       310        320        330        340        350        360 
VIKMPMVDPE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE 

       370        380        390        400        410        420 
KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STVNPMCYAL 

       430        440        450        460 
CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, sequence, and functional expression of the mouse M1 muscarinic acetylcholine receptor gene."
Shapiro R.A., Scherer N.M., Habecker B.A., Subers E.M., Nathanson N.M.
J. Biol. Chem. 263:18397-18403(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Erratum
Shapiro R.A., Scherer N.M., Habecker B.A., Subers E.M., Nathanson N.M.
J. Biol. Chem. 264:6596-6596(1989)
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Corpus striatum and Hypothalamus.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04192 Genomic DNA. Translation: AAA37158.1.
AK081248 mRNA. Translation: BAC38175.1.
AK138282 mRNA. Translation: BAE23612.1.
AC025794 Genomic DNA. No translation available.
CH466612 Genomic DNA. Translation: EDL33348.1.
CH466612 Genomic DNA. Translation: EDL33349.1.
BC094242 mRNA. Translation: AAH94242.1.
PIRA31897.
RefSeqNP_001106167.1. NM_001112697.1.
NP_031724.2. NM_007698.3.
UniGeneMm.240607.

3D structure databases

ProteinModelPortalP12657.
SMRP12657. Positions 23-434.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP12657.
ChEMBLCHEMBL2097162.
GuidetoPHARMACOLOGY13.

Protein family/group databases

TCDB9.A.14.3.2. the g-protein-coupled receptor (gpcr) family.
GPCRDBSearch...

PTM databases

PhosphoSiteP12657.

Proteomic databases

PaxDbP12657.
PRIDEP12657.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035444; ENSMUSP00000042632; ENSMUSG00000032773.
ENSMUST00000163785; ENSMUSP00000126103; ENSMUSG00000032773.
GeneID12669.
KEGGmmu:12669.
UCSCuc008gmf.2. mouse.

Organism-specific databases

CTD1128.
MGIMGI:88396. Chrm1.

Phylogenomic databases

eggNOGNOG250863.
GeneTreeENSGT00690000101881.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP12657.
KOK04129.
OMARCCRTPR.
PhylomeDBP12657.
TreeFamTF320495.

Gene expression databases

CleanExMM_CHRM1.
GenevestigatorP12657.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF59. PTHR24249:SF59. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281906.
PROP12657.
SOURCESearch...

Entry information

Entry nameACM1_MOUSE
AccessionPrimary (citable) accession number: P12657
Secondary accession number(s): Q52KQ0, Q8BJN3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries