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Protein

Muscarinic acetylcholine receptor M1

Gene

Chrm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-416476. G alpha (q) signalling events.

Protein family/group databases

TCDBi9.A.14.3.2. the g-protein-coupled receptor (gpcr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M1
Gene namesi
Name:Chrm1
Synonyms:Chrm-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:88396. Chrm1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24ExtracellularSequence analysisAdd BLAST24
Transmembranei25 – 47Helical; Name=1By similarityAdd BLAST23
Topological domaini48 – 61CytoplasmicSequence analysisAdd BLAST14
Transmembranei62 – 82Helical; Name=2By similarityAdd BLAST21
Topological domaini83 – 99ExtracellularSequence analysisAdd BLAST17
Transmembranei100 – 121Helical; Name=3By similarityAdd BLAST22
Topological domaini122 – 141CytoplasmicSequence analysisAdd BLAST20
Transmembranei142 – 164Helical; Name=4By similarityAdd BLAST23
Topological domaini165 – 186ExtracellularSequence analysisAdd BLAST22
Transmembranei187 – 209Helical; Name=5By similarityAdd BLAST23
Topological domaini210 – 366CytoplasmicSequence analysisAdd BLAST157
Transmembranei367 – 387Helical; Name=6By similarityAdd BLAST21
Topological domaini388 – 401ExtracellularSequence analysisAdd BLAST14
Transmembranei402 – 421Helical; Name=7By similarityAdd BLAST20
Topological domaini422 – 460CytoplasmicSequence analysisAdd BLAST39

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3733.
GuidetoPHARMACOLOGYi13.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690171 – 460Muscarinic acetylcholine receptor M1Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...)Curated1
Glycosylationi12N-linked (GlcNAc...)Curated1
Disulfide bondi98 ↔ 178PROSITE-ProRule annotation
Modified residuei230PhosphothreonineCombined sources1
Modified residuei254PhosphoserineBy similarity1
Modified residuei451PhosphoserineSequence analysis1
Modified residuei455PhosphothreonineSequence analysis1
Modified residuei457PhosphoserineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP12657.
PRIDEiP12657.

PTM databases

iPTMnetiP12657.
PhosphoSitePlusiP12657.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032773.
CleanExiMM_CHRM1.
ExpressionAtlasiP12657. baseline and differential.
GenevisibleiP12657. MM.

Interactioni

Subunit structurei

Interacts with GPRASP2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042632.

Chemistry databases

BindingDBiP12657.

Structurei

3D structure databases

ProteinModelPortaliP12657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP12657.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG091G06VI.
PhylomeDBiP12657.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF
60 70 80 90 100
KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL
110 120 130 140 150
WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW
160 170 180 190 200
LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP
210 220 230 240 250
VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG
260 270 280 290 300
SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
310 320 330 340 350
VIKMPMVDPE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA
360 370 380 390 400
KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW
410 420 430 440 450
ELGYWLCYVN STVNPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG
460
SVHRTPSRQC
Length:460
Mass (Da):51,379
Last modified:October 3, 2012 - v2
Checksum:i2F6A93DE8FF4F325
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30I → S in AAA37158 (PubMed:2848036).Curated1
Sequence conflicti50F → I in AAA37158 (PubMed:2848036).Curated1
Sequence conflicti420L → S in AAA37158 (PubMed:2848036).Curated1
Sequence conflicti428T → H in AAA37158 (PubMed:2848036).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04192 Genomic DNA. Translation: AAA37158.1.
AK081248 mRNA. Translation: BAC38175.1.
AK138282 mRNA. Translation: BAE23612.1.
AC025794 Genomic DNA. No translation available.
CH466612 Genomic DNA. Translation: EDL33348.1.
CH466612 Genomic DNA. Translation: EDL33349.1.
BC094242 mRNA. Translation: AAH94242.1.
CCDSiCCDS29539.1.
PIRiA31897.
RefSeqiNP_001106167.1. NM_001112697.1.
NP_031724.2. NM_007698.3.
UniGeneiMm.240607.

Genome annotation databases

EnsembliENSMUST00000035444; ENSMUSP00000042632; ENSMUSG00000032773.
ENSMUST00000163785; ENSMUSP00000126103; ENSMUSG00000032773.
GeneIDi12669.
KEGGimmu:12669.
UCSCiuc008gmf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04192 Genomic DNA. Translation: AAA37158.1.
AK081248 mRNA. Translation: BAC38175.1.
AK138282 mRNA. Translation: BAE23612.1.
AC025794 Genomic DNA. No translation available.
CH466612 Genomic DNA. Translation: EDL33348.1.
CH466612 Genomic DNA. Translation: EDL33349.1.
BC094242 mRNA. Translation: AAH94242.1.
CCDSiCCDS29539.1.
PIRiA31897.
RefSeqiNP_001106167.1. NM_001112697.1.
NP_031724.2. NM_007698.3.
UniGeneiMm.240607.

3D structure databases

ProteinModelPortaliP12657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042632.

Chemistry databases

BindingDBiP12657.
ChEMBLiCHEMBL3733.
GuidetoPHARMACOLOGYi13.

Protein family/group databases

TCDBi9.A.14.3.2. the g-protein-coupled receptor (gpcr) family.
GPCRDBiSearch...

PTM databases

iPTMnetiP12657.
PhosphoSitePlusiP12657.

Proteomic databases

PaxDbiP12657.
PRIDEiP12657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035444; ENSMUSP00000042632; ENSMUSG00000032773.
ENSMUST00000163785; ENSMUSP00000126103; ENSMUSG00000032773.
GeneIDi12669.
KEGGimmu:12669.
UCSCiuc008gmf.2. mouse.

Organism-specific databases

CTDi1128.
MGIiMGI:88396. Chrm1.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP12657.
KOiK04129.
OMAiRCCRTPR.
OrthoDBiEOG091G06VI.
PhylomeDBiP12657.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiP12657.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032773.
CleanExiMM_CHRM1.
ExpressionAtlasiP12657. baseline and differential.
GenevisibleiP12657. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002228. Musac_Ach_M1_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00538. MUSCRINICM1R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM1_MOUSE
AccessioniPrimary (citable) accession number: P12657
Secondary accession number(s): Q52KQ0, Q8BJN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.