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P12657

- ACM1_MOUSE

UniProt

P12657 - ACM1_MOUSE

Protein

Muscarinic acetylcholine receptor M1

Gene

Chrm1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

    GO - Molecular functioni

    1. drug binding Source: Ensembl
    2. G-protein coupled acetylcholine receptor activity Source: Ensembl

    GO - Biological processi

    1. cognition Source: InterPro
    2. neuromuscular synaptic transmission Source: Ensembl
    3. positive regulation of intracellular protein transport Source: Ensembl
    4. positive regulation of ion transport Source: MGI
    5. regulation of locomotion Source: MGI
    6. saliva secretion Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Protein family/group databases

    TCDBi9.A.14.3.2. the g-protein-coupled receptor (gpcr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M1
    Gene namesi
    Name:Chrm1
    Synonyms:Chrm-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:88396. Chrm1.

    Subcellular locationi

    GO - Cellular componenti

    1. asymmetric synapse Source: Ensembl
    2. axon terminus Source: Ensembl
    3. cell junction Source: UniProtKB-KW
    4. dendrite Source: Ensembl
    5. integral component of plasma membrane Source: InterPro
    6. postsynaptic density Source: Ensembl
    7. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Muscarinic acetylcholine receptor M1PRO_0000069017Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Curated
    Glycosylationi12 – 121N-linked (GlcNAc...)Curated
    Disulfide bondi98 ↔ 178PROSITE-ProRule annotation
    Modified residuei451 – 4511PhosphoserineSequence Analysis
    Modified residuei455 – 4551PhosphothreonineSequence Analysis
    Modified residuei457 – 4571PhosphoserineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP12657.
    PRIDEiP12657.

    PTM databases

    PhosphoSiteiP12657.

    Expressioni

    Gene expression databases

    CleanExiMM_CHRM1.
    GenevestigatoriP12657.

    Interactioni

    Subunit structurei

    Interacts with GPRASP2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP12657.
    SMRiP12657. Positions 23-242, 362-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini48 – 6114CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini83 – 9917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini122 – 14120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini165 – 18622ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini210 – 366157CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini388 – 40114ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini422 – 46039CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4723Helical; Name=1By similarityAdd
    BLAST
    Transmembranei62 – 8221Helical; Name=2By similarityAdd
    BLAST
    Transmembranei100 – 12122Helical; Name=3By similarityAdd
    BLAST
    Transmembranei142 – 16423Helical; Name=4By similarityAdd
    BLAST
    Transmembranei187 – 20923Helical; Name=5By similarityAdd
    BLAST
    Transmembranei367 – 38721Helical; Name=6By similarityAdd
    BLAST
    Transmembranei402 – 42120Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    GeneTreeiENSGT00690000101881.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiP12657.
    KOiK04129.
    OMAiRCCRTPR.
    PhylomeDBiP12657.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR002228. Musac_Ach_M1_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00538. MUSCRINICM1R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12657-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF    50
    KVNTELKTVN NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL 100
    WLALDYVASN ASVMNLLLIS FDRYFSVTRP LSYRAKRTPR RAALMIGLAW 150
    LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI QFLSQPIITF GTAMAAFYLP 200
    VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG 250
    SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 300
    VIKMPMVDPE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA 350
    KRKTFSLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW 400
    ELGYWLCYVN STVNPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG 450
    SVHRTPSRQC 460
    Length:460
    Mass (Da):51,379
    Last modified:October 3, 2012 - v2
    Checksum:i2F6A93DE8FF4F325
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301I → S in AAA37158. (PubMed:2848036)Curated
    Sequence conflicti50 – 501F → I in AAA37158. (PubMed:2848036)Curated
    Sequence conflicti420 – 4201L → S in AAA37158. (PubMed:2848036)Curated
    Sequence conflicti428 – 4281T → H in AAA37158. (PubMed:2848036)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04192 Genomic DNA. Translation: AAA37158.1.
    AK081248 mRNA. Translation: BAC38175.1.
    AK138282 mRNA. Translation: BAE23612.1.
    AC025794 Genomic DNA. No translation available.
    CH466612 Genomic DNA. Translation: EDL33348.1.
    CH466612 Genomic DNA. Translation: EDL33349.1.
    BC094242 mRNA. Translation: AAH94242.1.
    CCDSiCCDS29539.1.
    PIRiA31897.
    RefSeqiNP_001106167.1. NM_001112697.1.
    NP_031724.2. NM_007698.3.
    UniGeneiMm.240607.

    Genome annotation databases

    EnsembliENSMUST00000035444; ENSMUSP00000042632; ENSMUSG00000032773.
    ENSMUST00000163785; ENSMUSP00000126103; ENSMUSG00000032773.
    GeneIDi12669.
    KEGGimmu:12669.
    UCSCiuc008gmf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04192 Genomic DNA. Translation: AAA37158.1 .
    AK081248 mRNA. Translation: BAC38175.1 .
    AK138282 mRNA. Translation: BAE23612.1 .
    AC025794 Genomic DNA. No translation available.
    CH466612 Genomic DNA. Translation: EDL33348.1 .
    CH466612 Genomic DNA. Translation: EDL33349.1 .
    BC094242 mRNA. Translation: AAH94242.1 .
    CCDSi CCDS29539.1.
    PIRi A31897.
    RefSeqi NP_001106167.1. NM_001112697.1.
    NP_031724.2. NM_007698.3.
    UniGenei Mm.240607.

    3D structure databases

    ProteinModelPortali P12657.
    SMRi P12657. Positions 23-242, 362-430.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P12657.
    ChEMBLi CHEMBL3733.
    GuidetoPHARMACOLOGYi 13.

    Protein family/group databases

    TCDBi 9.A.14.3.2. the g-protein-coupled receptor (gpcr) family.
    GPCRDBi Search...

    PTM databases

    PhosphoSitei P12657.

    Proteomic databases

    PaxDbi P12657.
    PRIDEi P12657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035444 ; ENSMUSP00000042632 ; ENSMUSG00000032773 .
    ENSMUST00000163785 ; ENSMUSP00000126103 ; ENSMUSG00000032773 .
    GeneIDi 12669.
    KEGGi mmu:12669.
    UCSCi uc008gmf.2. mouse.

    Organism-specific databases

    CTDi 1128.
    MGIi MGI:88396. Chrm1.

    Phylogenomic databases

    eggNOGi NOG250863.
    GeneTreei ENSGT00690000101881.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi P12657.
    KOi K04129.
    OMAi RCCRTPR.
    PhylomeDBi P12657.
    TreeFami TF320495.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 281906.
    PROi P12657.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CHRM1.
    Genevestigatori P12657.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR002228. Musac_Ach_M1_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00538. MUSCRINICM1R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequence, and functional expression of the mouse M1 muscarinic acetylcholine receptor gene."
      Shapiro R.A., Scherer N.M., Habecker B.A., Subers E.M., Nathanson N.M.
      J. Biol. Chem. 263:18397-18403(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Erratum
      Shapiro R.A., Scherer N.M., Habecker B.A., Subers E.M., Nathanson N.M.
      J. Biol. Chem. 264:6596-6596(1989)
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Corpus striatum and Hypothalamus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiACM1_MOUSE
    AccessioniPrimary (citable) accession number: P12657
    Secondary accession number(s): Q52KQ0, Q8BJN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3