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Protein

Glutathione S-transferase 1

Gene

GST1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione transferase activity Source: AgBase

GO - Biological processi

  • response to herbicide Source: AgBase
  • response to hydrogen peroxide Source: AgBase
  • response to reactive oxygen species Source: AgBase
  • response to salicylic acid Source: AgBase
  • response to xenobiotic stimulus Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 6752.
SABIO-RKP12653.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 1 (EC:2.5.1.18)
Alternative name(s):
GST class-phi member 1
GST-29
GST-I
Gene namesi
Name:GST1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi65344.

Subcellular locationi

GO - Cellular componenti

  • protein complex Source: AgBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001858412 – 214Glutathione S-transferase 1Add BLAST213

Proteomic databases

PaxDbiP12653.
PRIDEiP12653.

Expressioni

Tissue specificityi

Expressed in the stem and leaves, lower levels are seen in the pollen and endosperm.

Interactioni

Subunit structurei

Homodimer or heterodimer of GST-I and GST-IV (=GST-II).2 Publications

Protein-protein interaction databases

STRINGi4577.GRMZM2G116273_P01.

Structurei

Secondary structure

1214
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi10 – 14Combined sources5
Helixi15 – 25Combined sources11
Beta strandi29 – 32Combined sources4
Turni36 – 39Combined sources4
Helixi40 – 42Combined sources3
Helixi44 – 47Combined sources4
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 79Combined sources12
Helixi81 – 84Combined sources4
Turni85 – 87Combined sources3
Helixi89 – 104Combined sources16
Helixi106 – 117Combined sources12
Helixi119 – 122Combined sources4
Helixi129 – 152Combined sources24
Beta strandi154 – 160Combined sources7
Helixi163 – 166Combined sources4
Helixi169 – 175Combined sources7
Helixi179 – 186Combined sources8
Helixi188 – 199Combined sources12
Helixi201 – 209Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXDX-ray2.50A/B2-210[»]
1BYEX-ray2.80A/B/C/D2-214[»]
ProteinModelPortaliP12653.
SMRiP12653.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12653.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 83GST N-terminalAdd BLAST82
Domaini88 – 214GST C-terminalAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 42Glutathione binding2
Regioni54 – 55Glutathione binding2
Regioni67 – 68Glutathione binding2

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
KOiK00799.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12653-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPMKLYGAV MSWNLTRCAT ALEEAGSDYE IVPINFATAE HKSPEHLVRN
60 70 80 90 100
PFGQVPALQD GDLYLFESRA ICKYAARKNK PELLREGNLE EAAMVDVWIE
110 120 130 140 150
VEANQYTAAL NPILFQVLIS PMLGGTTDQK VVDENLEKLK KVLEVYEARL
160 170 180 190 200
TKCKYLAGDF LSLADLNHVS VTLCLFATPY ASVLDAYPHV KAWWSGLMER
210
PSVQKVAALM KPSA
Length:214
Mass (Da):23,822
Last modified:January 23, 2007 - v4
Checksum:i97DA6337ADF03CB1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15L → V in AAA33470 (Ref. 2) Curated1
Sequence conflicti15L → V in AAA33469 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06754 mRNA. Translation: CAA29928.1.
M16901 mRNA. Translation: AAA33470.1.
M16902, M16900 Genomic DNA. Translation: AAA33469.1.
PIRiS03726. XUZM1.
RefSeqiNP_001105412.1. NM_001111942.1.
UniGeneiZm.9.

Genome annotation databases

GeneIDi542366.
KEGGizma:542366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06754 mRNA. Translation: CAA29928.1.
M16901 mRNA. Translation: AAA33470.1.
M16902, M16900 Genomic DNA. Translation: AAA33469.1.
PIRiS03726. XUZM1.
RefSeqiNP_001105412.1. NM_001111942.1.
UniGeneiZm.9.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXDX-ray2.50A/B2-210[»]
1BYEX-ray2.80A/B/C/D2-214[»]
ProteinModelPortaliP12653.
SMRiP12653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G116273_P01.

Proteomic databases

PaxDbiP12653.
PRIDEiP12653.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi542366.
KEGGizma:542366.

Organism-specific databases

MaizeGDBi65344.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
HOGENOMiHOG000125746.
KOiK00799.

Enzyme and pathway databases

BRENDAi2.5.1.18. 6752.
SABIO-RKP12653.

Miscellaneous databases

EvolutionaryTraceiP12653.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTF1_MAIZE
AccessioniPrimary (citable) accession number: P12653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 112 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.