Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P12646

- G6PD_DROME

UniProt

P12646 - G6PD_DROME

Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

Zw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity.By similarity

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761NADP 1By similarity
    Binding sitei175 – 1751NADP 1; via carbonyl oxygenBy similarity
    Binding sitei175 – 1751SubstrateBy similarity
    Binding sitei243 – 2431SubstrateBy similarity
    Binding sitei262 – 2621SubstrateBy similarity
    Active sitei267 – 2671Proton acceptorBy similarity
    Binding sitei362 – 3621NADP 2By similarity
    Binding sitei365 – 3651SubstrateBy similarity
    Binding sitei370 – 3701SubstrateBy similarity
    Binding sitei371 – 3711NADP 2By similarity
    Binding sitei375 – 3751NADP 2By similarity
    Binding sitei398 – 3981NADP 2By similarity
    Binding sitei400 – 4001SubstrateBy similarity
    Binding sitei492 – 4921NADP 2By similarity
    Binding sitei508 – 5081NADP 2By similarity
    Binding sitei514 – 5141NADP 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi42 – 498NADP 1By similarity
    Nucleotide bindingi406 – 4083NADP 2By similarity
    Nucleotide bindingi426 – 4283NADP 2By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: FlyBase
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glucose 6-phosphate metabolic process Source: UniProtKB
    2. NADP metabolic process Source: UniProtKB
    3. pentose-phosphate shunt Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_210042. Pentose phosphate pathway (hexose monophosphate shunt).
    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
    Short name:
    G6PD
    Alternative name(s):
    Zwischenferment
    Gene namesi
    Name:Zw
    Synonyms:G6PD
    ORF Names:CG12529
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0004057. Zw.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Glucose-6-phosphate 1-dehydrogenasePRO_0000068092Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP12646.
    PRIDEiP12646.

    Expressioni

    Gene expression databases

    BgeeiP12646.

    Interactioni

    Protein-protein interaction databases

    BioGridi59270. 1 interaction.
    DIPiDIP-20748N.
    IntActiP12646. 2 interactions.
    MINTiMINT-882769.

    Structurei

    3D structure databases

    ProteinModelPortaliP12646.
    SMRiP12646. Positions 36-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni205 – 2095Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0364.
    InParanoidiP12646.
    KOiK00036.
    OrthoDBiEOG7DRJ2T.
    PhylomeDBiP12646.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P12646-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATQKEDHTA LDLIIKSLKS PTMVCEGTHF DGKIPHTFVI FGASGDLAKK    50
    KIYPTLWWLY RDDLLPKPTK FCGYARSMLT VDSIKEQCLP YMKVQPHEQK 100
    KYEEFWALNE YVSGRYDGRT GFELLNQQLE IMENKNKANR IFYLALPPSV 150
    FEEVTVNIKQ ICMSVCGWNR VIIEKPFGRD DASSQALSDH LAGLFQEDQL 200
    YRIDHYLGKE MVQNLMTIRF GNKILSSTWN RENIASVLIT FKEPFGTQGR 250
    GGYFDEFGII RDVMQNHLLQ ILSLVAMEKP VSCHPDDIRD EKVKVLKSIE 300
    ALTLDDMVLG QYLGNPQGTN DDARTGYVED PTVSNDSNTP TYALGVLKIN 350
    NERWQGVPFI LRCGKALNER KAEVRIQYQD VLGDIFEGNT KRNELVIRVQ 400
    PGEALYFKMM TKSPGITFDI EETELDLTYE HRYKDSYLPD AYERLILDVF 450
    CGSQMHFVRS DELREAWRIF TPILHQIEKE HIRPITYQYG SRGPKEADRK 500
    CEENNFKYSG SYKWHGGKAA TSNH 524
    Length:524
    Mass (Da):60,431
    Last modified:July 15, 1998 - v2
    Checksum:iABF81B763A82F1FD
    GO
    Isoform B (identifier: P12646-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-22: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:502
    Mass (Da):58,008
    Checksum:iDFF55A83782BEDAC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti185 – 1862QA → AG in AAA51463. (PubMed:2838391)Curated
    Sequence conflicti214 – 2141N → K in AAA51463. (PubMed:2838391)Curated
    Sequence conflicti344 – 3463LGV → ARS in AAA51463. (PubMed:2838391)Curated
    Sequence conflicti461 – 4655DELRE → AAAQ in AAA51463. (PubMed:2838391)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti32 – 321G → C in strain: F24.1, MT32 and MT68.
    Natural varianti80 – 801T → N in strain: Z74.
    Natural varianti382 – 3821L → P in strain: F23.3, MT41, Z3, Z5, Z11, Z16, Z21, Z27, Z42, Z55, Z64, Z74 and Berkeley.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2222Missing in isoform B. CuratedVSP_001593Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26674, M26673 Genomic DNA. Translation: AAA51463.1.
    U42738 Genomic DNA. Translation: AAB02801.1.
    U42739 Genomic DNA. Translation: AAB02802.1.
    U42740 Genomic DNA. Translation: AAB02803.1.
    U42741 Genomic DNA. Translation: AAB02804.1.
    U42742 Genomic DNA. Translation: AAB02805.1.
    U42743 Genomic DNA. Translation: AAB02806.1.
    U42744 Genomic DNA. Translation: AAB02807.1.
    U42745 Genomic DNA. Translation: AAB02808.1.
    U42746 Genomic DNA. Translation: AAB02809.1.
    U42747 Genomic DNA. Translation: AAB02810.1.
    U42748 Genomic DNA. Translation: AAB02811.1.
    U42749 Genomic DNA. Translation: AAB02812.1.
    U43165 Genomic DNA. Translation: AAA99071.1.
    U43166 Genomic DNA. Translation: AAA99072.1.
    U43167 Genomic DNA. Translation: AAA99073.1.
    U44721 Genomic DNA. Translation: AAA99092.1.
    U45985 Genomic DNA. Translation: AAA99107.1.
    AE014298 Genomic DNA. Translation: AAF48999.1.
    AE014298 Genomic DNA. Translation: AAF49000.2.
    AY052079 mRNA. Translation: AAK93503.1.
    PIRiA47740.
    JT0272. DEFFG6.
    RefSeqiNP_523411.1. NM_078687.1.
    NP_728287.1. NM_167676.1.
    UniGeneiDm.225.

    Genome annotation databases

    GeneIDi32974.
    KEGGidme:Dmel_CG12529.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26674 , M26673 Genomic DNA. Translation: AAA51463.1 .
    U42738 Genomic DNA. Translation: AAB02801.1 .
    U42739 Genomic DNA. Translation: AAB02802.1 .
    U42740 Genomic DNA. Translation: AAB02803.1 .
    U42741 Genomic DNA. Translation: AAB02804.1 .
    U42742 Genomic DNA. Translation: AAB02805.1 .
    U42743 Genomic DNA. Translation: AAB02806.1 .
    U42744 Genomic DNA. Translation: AAB02807.1 .
    U42745 Genomic DNA. Translation: AAB02808.1 .
    U42746 Genomic DNA. Translation: AAB02809.1 .
    U42747 Genomic DNA. Translation: AAB02810.1 .
    U42748 Genomic DNA. Translation: AAB02811.1 .
    U42749 Genomic DNA. Translation: AAB02812.1 .
    U43165 Genomic DNA. Translation: AAA99071.1 .
    U43166 Genomic DNA. Translation: AAA99072.1 .
    U43167 Genomic DNA. Translation: AAA99073.1 .
    U44721 Genomic DNA. Translation: AAA99092.1 .
    U45985 Genomic DNA. Translation: AAA99107.1 .
    AE014298 Genomic DNA. Translation: AAF48999.1 .
    AE014298 Genomic DNA. Translation: AAF49000.2 .
    AY052079 mRNA. Translation: AAK93503.1 .
    PIRi A47740.
    JT0272. DEFFG6.
    RefSeqi NP_523411.1. NM_078687.1.
    NP_728287.1. NM_167676.1.
    UniGenei Dm.225.

    3D structure databases

    ProteinModelPortali P12646.
    SMRi P12646. Positions 36-514.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 59270. 1 interaction.
    DIPi DIP-20748N.
    IntActi P12646. 2 interactions.
    MINTi MINT-882769.

    Proteomic databases

    PaxDbi P12646.
    PRIDEi P12646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 32974.
    KEGGi dme:Dmel_CG12529.

    Organism-specific databases

    CTDi 32974.
    FlyBasei FBgn0004057. Zw.

    Phylogenomic databases

    eggNOGi COG0364.
    InParanoidi P12646.
    KOi K00036.
    OrthoDBi EOG7DRJ2T.
    PhylomeDBi P12646.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .
    Reactomei REACT_210042. Pentose phosphate pathway (hexose monophosphate shunt).

    Miscellaneous databases

    GenomeRNAii 32974.
    NextBioi 781313.

    Gene expression databases

    Bgeei P12646.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene."
      Fouts D., Ganguly R., Gutierrez A.G., Lucchesi J.C., Manning J.E.
      Gene 63:261-275(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
    2. "Historical selection, amino acid polymorphism and lineage-specific divergence at the G6pd locus in Drosophila melanogaster and D. simulans."
      Eanes W.F., Kirchner M., Yoon J., Biermann C.H., Wang I.N., McCartney M.A., Verrelli B.C.
      Genetics 144:1027-1041(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
      Strain: F23.3, F24.1, MT32, MT41, MT68, Z11, Z16, Z21, Z27, Z3, Z41, Z42, Z5, Z55, Z62, Z64 and Z74.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Embryo.
    6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiG6PD_DROME
    AccessioniPrimary (citable) accession number: P12646
    Secondary accession number(s): Q27574
    , Q27872, Q27879, Q27881, Q9VWE2, Q9VWE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3