Reviewed,
UniProtKB/Swiss-Prot P12646 (G6PD_DROME)
Last modified
November 25, 2008.
Version 92.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glucose-6-phosphate 1-dehydrogenase Short name=G6PD EC=1.1.1.49 Alternative name(s): Zwischenferment | ||||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||||
| Taxonomic identifier | 7227 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 524 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH. |
| Pathway | |
| Sequence similarities | Belongs to the glucose-6-phosphate dehydrogenase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Glucose metabolism |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform A (identifier: P12646-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform B (identifier: P12646-2) The sequence of this isoform differs from the canonical sequence as follows: 1-22: Missing. | ||||||
| Notes: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 524 | 524 | Glucose-6-phosphate 1-dehydrogenase | PRO_0000068092 | |||||
Sites | |||||||||
| Active site | 267 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 44 | 1 | NADP By similarity | ||||||
| Binding site | 76 | 1 | NADP By similarity | ||||||
| Binding site | 205 | 1 | Substrate By similarity | ||||||
| Binding site | 209 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 20 | 1 | Phosphoserine | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 22 | 22 | Missing in isoform B. | VSP_001593 | |||||
| Natural variant | 32 | 1 | G → C in strain: F24.1, MT32 and MT68. | ||||||
| Natural variant | 80 | 1 | T → N in strain: Z74. | ||||||
| Natural variant | 382 | 1 | L → P in strain: F23.3, MT41, Z3, Z5, Z11, Z16, Z21, Z27, Z42, Z55, Z64, Z74 and Berkeley. | ||||||
Experimental info | |||||||||
| Sequence conflict | 185 – 186 | 2 | QA → AG in AAA51463. Ref.1 | ||||||
| Sequence conflict | 214 | 1 | N → K in AAA51463. Ref.1 | ||||||
| Sequence conflict | 344 – 346 | 3 | LGV → ARS in AAA51463. Ref.1 | ||||||
| Sequence conflict | 461 – 465 | 5 | DELRE → AAAQ in AAA51463. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene." Fouts D., Ganguly R., Gutierrez A.G., Lucchesi J.C., Manning J.E. Gene 63:261-275(1988) [PubMed: 2838391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A). |
| [2] | "Historical selection, amino acid polymorphism and lineage-specific divergence at the G6pd locus in Drosophila melanogaster and D. simulans." Eanes W.F., Kirchner M., Yoon J., Biermann C.H., Wang I.N., McCartney M.A., Verrelli B.C. Genetics 144:1027-1041(1996) [PubMed: 8913747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A). Strain: F23.3, F24.1, MT32, MT41, MT68, Z11, Z16, Z21, Z27, Z3, Z41, Z42, Z5, Z55, Z62, Z64 and Z74. |
| [3] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [4] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. |
| [5] | "A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo. |
| [6] | "Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, MASS SPECTROMETRY. Tissue: Embryo. |
Cross-references
Sequence databases | |
|---|---|
| M26674, M26673 Genomic DNA. Translation: AAA51463.1. U42738 Genomic DNA. Translation: AAB02801.1. U42739 Genomic DNA. Translation: AAB02802.1. U42740 Genomic DNA. Translation: AAB02803.1. U42741 Genomic DNA. Translation: AAB02804.1. U42742 Genomic DNA. Translation: AAB02805.1. U42743 Genomic DNA. Translation: AAB02806.1. U42744 Genomic DNA. Translation: AAB02807.1. U42745 Genomic DNA. Translation: AAB02808.1. U42746 Genomic DNA. Translation: AAB02809.1. U42747 Genomic DNA. Translation: AAB02810.1. U42748 Genomic DNA. Translation: AAB02811.1. U42749 Genomic DNA. Translation: AAB02812.1. U43165 Genomic DNA. Translation: AAA99071.1. U43166 Genomic DNA. Translation: AAA99072.1. U43167 Genomic DNA. Translation: AAA99073.1. U44721 Genomic DNA. Translation: AAA99092.1. U45985 Genomic DNA. Translation: AAA99107.1. AE014298 Genomic DNA. Translation: AAF48999.1. AE014298 Genomic DNA. Translation: AAF49000.2. AY052079 mRNA. Translation: AAK93503.1. | |
| PIR | A47740. DEFFG6. JT0272. |
| RefSeq | NP_523411.1. NP_728287.1. |
| UniGene | Dm.225 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1QKI based on UniProtKB P11413. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:20748N. |
| IntAct | P12646. |
Genome annotation databases | |
| Ensembl | CG12529. Drosophila melanogaster. [Contig view] |
| GeneID | 32974. |
| KEGG | dme:Dmel_CG12529. |
Organism-specific databases | |
| FlyBase | FBgn0004057. Zw. |
Gene expression databases | |
| ArrayExpress | P12646. |
| GermOnline | CG12529. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR23429. G6PDH. 1 hit. |
| Pfam | PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000110. G6PD. 1 hit. |
| PRINTS | PR00079. G6PDHDRGNASE. |
| ProDom | PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR00871. zwf. 1 hit. |
| PROSITE | PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 781313. |
Entry information
| Entry name | G6PD_DROME | ||||||||
| Accession | Primary (citable) accession number: P12646 Secondary accession number(s): Q27574 Q9VWE3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


