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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

Zw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (Zw), Glucose-6-phosphate 1-dehydrogenase (Zw-RA), Glucose-6-phosphate 1-dehydrogenase (Dmel\CG7140), Glucose-6-phosphate 1-dehydrogenase (Zw)
  2. Probable 6-phosphogluconolactonase (CG17333)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (Pgd), 6-phosphogluconate dehydrogenase, decarboxylating (Pgd), 6-phosphogluconate dehydrogenase, decarboxylating (Pgd)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76NADP 1By similarity1
Binding sitei175NADP 1; via carbonyl oxygenBy similarity1
Binding sitei175SubstrateBy similarity1
Binding sitei243SubstrateBy similarity1
Binding sitei262SubstrateBy similarity1
Active sitei267Proton acceptorBy similarity1
Binding sitei362NADP 2By similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei370SubstrateBy similarity1
Binding sitei371NADP 2By similarity1
Binding sitei375NADP 2By similarity1
Binding sitei398NADP 2By similarity1
Binding sitei400SubstrateBy similarity1
Binding sitei492NADP 2By similarity1
Binding sitei508NADP 2By similarity1
Binding sitei514NADP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 49NADP 1By similarity8
Nucleotide bindingi406 – 408NADP 2By similarity3
Nucleotide bindingi426 – 428NADP 2By similarity3

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: FlyBase
  • NADP binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

ReactomeiR-DME-5628897. TP53 Regulates Metabolic Genes.
R-DME-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Alternative name(s):
Zwischenferment
Gene namesi
Name:Zw
Synonyms:G6PD
ORF Names:CG12529
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004057. Zw.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000680921 – 524Glucose-6-phosphate 1-dehydrogenaseAdd BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP12646.
PRIDEiP12646.

PTM databases

iPTMnetiP12646.

Expressioni

Gene expression databases

BgeeiFBgn0004057.
ExpressionAtlasiP12646. differential.
GenevisibleiP12646. DM.

Interactioni

Protein-protein interaction databases

BioGridi59270. 1 interactor.
DIPiDIP-20748N.
IntActiP12646. 2 interactors.
MINTiMINT-882769.
STRINGi7227.FBpp0074517.

Structurei

3D structure databases

ProteinModelPortaliP12646.
SMRiP12646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 209Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
COG0364. LUCA.
InParanoidiP12646.
KOiK00036.
OrthoDBiEOG091G06FN.
PhylomeDBiP12646.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiView protein in InterPro
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiView protein in Pfam
PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiView protein in PROSITE
PS00069. G6P_DEHYDROGENASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P12646-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATQKEDHTA LDLIIKSLKS PTMVCEGTHF DGKIPHTFVI FGASGDLAKK
60 70 80 90 100
KIYPTLWWLY RDDLLPKPTK FCGYARSMLT VDSIKEQCLP YMKVQPHEQK
110 120 130 140 150
KYEEFWALNE YVSGRYDGRT GFELLNQQLE IMENKNKANR IFYLALPPSV
160 170 180 190 200
FEEVTVNIKQ ICMSVCGWNR VIIEKPFGRD DASSQALSDH LAGLFQEDQL
210 220 230 240 250
YRIDHYLGKE MVQNLMTIRF GNKILSSTWN RENIASVLIT FKEPFGTQGR
260 270 280 290 300
GGYFDEFGII RDVMQNHLLQ ILSLVAMEKP VSCHPDDIRD EKVKVLKSIE
310 320 330 340 350
ALTLDDMVLG QYLGNPQGTN DDARTGYVED PTVSNDSNTP TYALGVLKIN
360 370 380 390 400
NERWQGVPFI LRCGKALNER KAEVRIQYQD VLGDIFEGNT KRNELVIRVQ
410 420 430 440 450
PGEALYFKMM TKSPGITFDI EETELDLTYE HRYKDSYLPD AYERLILDVF
460 470 480 490 500
CGSQMHFVRS DELREAWRIF TPILHQIEKE HIRPITYQYG SRGPKEADRK
510 520
CEENNFKYSG SYKWHGGKAA TSNH
Length:524
Mass (Da):60,431
Last modified:July 15, 1998 - v2
Checksum:iABF81B763A82F1FD
GO
Isoform B (identifier: P12646-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: No experimental confirmation available.
Show »
Length:502
Mass (Da):58,008
Checksum:iDFF55A83782BEDAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185 – 186QA → AG in AAA51463 (PubMed:2838391).Curated2
Sequence conflicti214N → K in AAA51463 (PubMed:2838391).Curated1
Sequence conflicti344 – 346LGV → ARS in AAA51463 (PubMed:2838391).Curated3
Sequence conflicti461 – 465DELRE → AAAQ in AAA51463 (PubMed:2838391).Curated5

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti32G → C in strain: F24.1, MT32 and MT68. 1
Natural varianti80T → N in strain: Z74. 1
Natural varianti382L → P in strain: F23.3, MT41, Z3, Z5, Z11, Z16, Z21, Z27, Z42, Z55, Z64, Z74 and Berkeley. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0015931 – 22Missing in isoform B. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26674, M26673 Genomic DNA. Translation: AAA51463.1.
U42738 Genomic DNA. Translation: AAB02801.1.
U42739 Genomic DNA. Translation: AAB02802.1.
U42740 Genomic DNA. Translation: AAB02803.1.
U42741 Genomic DNA. Translation: AAB02804.1.
U42742 Genomic DNA. Translation: AAB02805.1.
U42743 Genomic DNA. Translation: AAB02806.1.
U42744 Genomic DNA. Translation: AAB02807.1.
U42745 Genomic DNA. Translation: AAB02808.1.
U42746 Genomic DNA. Translation: AAB02809.1.
U42747 Genomic DNA. Translation: AAB02810.1.
U42748 Genomic DNA. Translation: AAB02811.1.
U42749 Genomic DNA. Translation: AAB02812.1.
U43165 Genomic DNA. Translation: AAA99071.1.
U43166 Genomic DNA. Translation: AAA99072.1.
U43167 Genomic DNA. Translation: AAA99073.1.
U44721 Genomic DNA. Translation: AAA99092.1.
U45985 Genomic DNA. Translation: AAA99107.1.
AE014298 Genomic DNA. Translation: AAF48999.1.
AE014298 Genomic DNA. Translation: AAF49000.2.
AY052079 mRNA. Translation: AAK93503.1.
PIRiA47740.
JT0272. DEFFG6.
RefSeqiNP_001285456.1. NM_001298527.1.
NP_523411.1. NM_078687.2.
NP_728287.1. NM_167676.2.
UniGeneiDm.225.

Genome annotation databases

GeneIDi32974.
KEGGidme:Dmel_CG12529.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26674, M26673 Genomic DNA. Translation: AAA51463.1.
U42738 Genomic DNA. Translation: AAB02801.1.
U42739 Genomic DNA. Translation: AAB02802.1.
U42740 Genomic DNA. Translation: AAB02803.1.
U42741 Genomic DNA. Translation: AAB02804.1.
U42742 Genomic DNA. Translation: AAB02805.1.
U42743 Genomic DNA. Translation: AAB02806.1.
U42744 Genomic DNA. Translation: AAB02807.1.
U42745 Genomic DNA. Translation: AAB02808.1.
U42746 Genomic DNA. Translation: AAB02809.1.
U42747 Genomic DNA. Translation: AAB02810.1.
U42748 Genomic DNA. Translation: AAB02811.1.
U42749 Genomic DNA. Translation: AAB02812.1.
U43165 Genomic DNA. Translation: AAA99071.1.
U43166 Genomic DNA. Translation: AAA99072.1.
U43167 Genomic DNA. Translation: AAA99073.1.
U44721 Genomic DNA. Translation: AAA99092.1.
U45985 Genomic DNA. Translation: AAA99107.1.
AE014298 Genomic DNA. Translation: AAF48999.1.
AE014298 Genomic DNA. Translation: AAF49000.2.
AY052079 mRNA. Translation: AAK93503.1.
PIRiA47740.
JT0272. DEFFG6.
RefSeqiNP_001285456.1. NM_001298527.1.
NP_523411.1. NM_078687.2.
NP_728287.1. NM_167676.2.
UniGeneiDm.225.

3D structure databases

ProteinModelPortaliP12646.
SMRiP12646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59270. 1 interactor.
DIPiDIP-20748N.
IntActiP12646. 2 interactors.
MINTiMINT-882769.
STRINGi7227.FBpp0074517.

PTM databases

iPTMnetiP12646.

Proteomic databases

PaxDbiP12646.
PRIDEiP12646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi32974.
KEGGidme:Dmel_CG12529.

Organism-specific databases

CTDi32974.
FlyBaseiFBgn0004057. Zw.

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
COG0364. LUCA.
InParanoidiP12646.
KOiK00036.
OrthoDBiEOG091G06FN.
PhylomeDBiP12646.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
ReactomeiR-DME-5628897. TP53 Regulates Metabolic Genes.
R-DME-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

GenomeRNAii32974.
PROiP12646.

Gene expression databases

BgeeiFBgn0004057.
ExpressionAtlasiP12646. differential.
GenevisibleiP12646. DM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiView protein in InterPro
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiView protein in Pfam
PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiView protein in PROSITE
PS00069. G6P_DEHYDROGENASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD_DROME
AccessioniPrimary (citable) accession number: P12646
Secondary accession number(s): Q27574
, Q27872, Q27879, Q27881, Q9VWE2, Q9VWE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: March 15, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.