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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

Zw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathway: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (CG7140), Glucose-6-phosphate 1-dehydrogenase (Zw), Glucose-6-phosphate 1-dehydrogenase (Zw-RA), Glucose-6-phosphate 1-dehydrogenase (CG7140), Glucose-6-phosphate 1-dehydrogenase (Zw)
  2. Probable 6-phosphogluconolactonase (CG17333)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (Pgd), 6-phosphogluconate dehydrogenase, decarboxylating (Pgd)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761NADP 1By similarity
Binding sitei175 – 1751NADP 1; via carbonyl oxygenBy similarity
Binding sitei175 – 1751SubstrateBy similarity
Binding sitei243 – 2431SubstrateBy similarity
Binding sitei262 – 2621SubstrateBy similarity
Active sitei267 – 2671Proton acceptorBy similarity
Binding sitei362 – 3621NADP 2By similarity
Binding sitei365 – 3651SubstrateBy similarity
Binding sitei370 – 3701SubstrateBy similarity
Binding sitei371 – 3711NADP 2By similarity
Binding sitei375 – 3751NADP 2By similarity
Binding sitei398 – 3981NADP 2By similarity
Binding sitei400 – 4001SubstrateBy similarity
Binding sitei492 – 4921NADP 2By similarity
Binding sitei508 – 5081NADP 2By similarity
Binding sitei514 – 5141NADP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 498NADP 1By similarity
Nucleotide bindingi406 – 4083NADP 2By similarity
Nucleotide bindingi426 – 4283NADP 2By similarity

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: FlyBase
  • NADP binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_298383. Pentose phosphate pathway (hexose monophosphate shunt).
REACT_358894. TP53 Regulates Metabolic Genes.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Alternative name(s):
Zwischenferment
Gene namesi
Name:Zw
Synonyms:G6PD
ORF Names:CG12529
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004057. Zw.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Glucose-6-phosphate 1-dehydrogenasePRO_0000068092Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP12646.
PRIDEiP12646.

Expressioni

Gene expression databases

BgeeiP12646.
ExpressionAtlasiP12646. differential.
GenevisibleiP12646. DM.

Interactioni

Protein-protein interaction databases

BioGridi59270. 1 interaction.
DIPiDIP-20748N.
IntActiP12646. 2 interactions.
MINTiMINT-882769.
STRINGi7227.FBpp0074517.

Structurei

3D structure databases

ProteinModelPortaliP12646.
SMRiP12646. Positions 36-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 2095Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0364.
InParanoidiP12646.
KOiK00036.
OrthoDBiEOG7DRJ2T.
PhylomeDBiP12646.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P12646-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATQKEDHTA LDLIIKSLKS PTMVCEGTHF DGKIPHTFVI FGASGDLAKK
60 70 80 90 100
KIYPTLWWLY RDDLLPKPTK FCGYARSMLT VDSIKEQCLP YMKVQPHEQK
110 120 130 140 150
KYEEFWALNE YVSGRYDGRT GFELLNQQLE IMENKNKANR IFYLALPPSV
160 170 180 190 200
FEEVTVNIKQ ICMSVCGWNR VIIEKPFGRD DASSQALSDH LAGLFQEDQL
210 220 230 240 250
YRIDHYLGKE MVQNLMTIRF GNKILSSTWN RENIASVLIT FKEPFGTQGR
260 270 280 290 300
GGYFDEFGII RDVMQNHLLQ ILSLVAMEKP VSCHPDDIRD EKVKVLKSIE
310 320 330 340 350
ALTLDDMVLG QYLGNPQGTN DDARTGYVED PTVSNDSNTP TYALGVLKIN
360 370 380 390 400
NERWQGVPFI LRCGKALNER KAEVRIQYQD VLGDIFEGNT KRNELVIRVQ
410 420 430 440 450
PGEALYFKMM TKSPGITFDI EETELDLTYE HRYKDSYLPD AYERLILDVF
460 470 480 490 500
CGSQMHFVRS DELREAWRIF TPILHQIEKE HIRPITYQYG SRGPKEADRK
510 520
CEENNFKYSG SYKWHGGKAA TSNH
Length:524
Mass (Da):60,431
Last modified:July 15, 1998 - v2
Checksum:iABF81B763A82F1FD
GO
Isoform B (identifier: P12646-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: No experimental confirmation available.
Show »
Length:502
Mass (Da):58,008
Checksum:iDFF55A83782BEDAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1862QA → AG in AAA51463 (PubMed:2838391).Curated
Sequence conflicti214 – 2141N → K in AAA51463 (PubMed:2838391).Curated
Sequence conflicti344 – 3463LGV → ARS in AAA51463 (PubMed:2838391).Curated
Sequence conflicti461 – 4655DELRE → AAAQ in AAA51463 (PubMed:2838391).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti32 – 321G → C in strain: F24.1, MT32 and MT68.
Natural varianti80 – 801T → N in strain: Z74.
Natural varianti382 – 3821L → P in strain: F23.3, MT41, Z3, Z5, Z11, Z16, Z21, Z27, Z42, Z55, Z64, Z74 and Berkeley.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222Missing in isoform B. CuratedVSP_001593Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26674, M26673 Genomic DNA. Translation: AAA51463.1.
U42738 Genomic DNA. Translation: AAB02801.1.
U42739 Genomic DNA. Translation: AAB02802.1.
U42740 Genomic DNA. Translation: AAB02803.1.
U42741 Genomic DNA. Translation: AAB02804.1.
U42742 Genomic DNA. Translation: AAB02805.1.
U42743 Genomic DNA. Translation: AAB02806.1.
U42744 Genomic DNA. Translation: AAB02807.1.
U42745 Genomic DNA. Translation: AAB02808.1.
U42746 Genomic DNA. Translation: AAB02809.1.
U42747 Genomic DNA. Translation: AAB02810.1.
U42748 Genomic DNA. Translation: AAB02811.1.
U42749 Genomic DNA. Translation: AAB02812.1.
U43165 Genomic DNA. Translation: AAA99071.1.
U43166 Genomic DNA. Translation: AAA99072.1.
U43167 Genomic DNA. Translation: AAA99073.1.
U44721 Genomic DNA. Translation: AAA99092.1.
U45985 Genomic DNA. Translation: AAA99107.1.
AE014298 Genomic DNA. Translation: AAF48999.1.
AE014298 Genomic DNA. Translation: AAF49000.2.
AY052079 mRNA. Translation: AAK93503.1.
PIRiA47740.
JT0272. DEFFG6.
RefSeqiNP_001285456.1. NM_001298527.1.
NP_523411.1. NM_078687.2.
NP_728287.1. NM_167676.2.
UniGeneiDm.225.

Genome annotation databases

GeneIDi32974.
KEGGidme:Dmel_CG12529.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26674, M26673 Genomic DNA. Translation: AAA51463.1.
U42738 Genomic DNA. Translation: AAB02801.1.
U42739 Genomic DNA. Translation: AAB02802.1.
U42740 Genomic DNA. Translation: AAB02803.1.
U42741 Genomic DNA. Translation: AAB02804.1.
U42742 Genomic DNA. Translation: AAB02805.1.
U42743 Genomic DNA. Translation: AAB02806.1.
U42744 Genomic DNA. Translation: AAB02807.1.
U42745 Genomic DNA. Translation: AAB02808.1.
U42746 Genomic DNA. Translation: AAB02809.1.
U42747 Genomic DNA. Translation: AAB02810.1.
U42748 Genomic DNA. Translation: AAB02811.1.
U42749 Genomic DNA. Translation: AAB02812.1.
U43165 Genomic DNA. Translation: AAA99071.1.
U43166 Genomic DNA. Translation: AAA99072.1.
U43167 Genomic DNA. Translation: AAA99073.1.
U44721 Genomic DNA. Translation: AAA99092.1.
U45985 Genomic DNA. Translation: AAA99107.1.
AE014298 Genomic DNA. Translation: AAF48999.1.
AE014298 Genomic DNA. Translation: AAF49000.2.
AY052079 mRNA. Translation: AAK93503.1.
PIRiA47740.
JT0272. DEFFG6.
RefSeqiNP_001285456.1. NM_001298527.1.
NP_523411.1. NM_078687.2.
NP_728287.1. NM_167676.2.
UniGeneiDm.225.

3D structure databases

ProteinModelPortaliP12646.
SMRiP12646. Positions 36-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59270. 1 interaction.
DIPiDIP-20748N.
IntActiP12646. 2 interactions.
MINTiMINT-882769.
STRINGi7227.FBpp0074517.

Proteomic databases

PaxDbiP12646.
PRIDEiP12646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi32974.
KEGGidme:Dmel_CG12529.

Organism-specific databases

CTDi32974.
FlyBaseiFBgn0004057. Zw.

Phylogenomic databases

eggNOGiCOG0364.
InParanoidiP12646.
KOiK00036.
OrthoDBiEOG7DRJ2T.
PhylomeDBiP12646.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
ReactomeiREACT_298383. Pentose phosphate pathway (hexose monophosphate shunt).
REACT_358894. TP53 Regulates Metabolic Genes.

Miscellaneous databases

GenomeRNAii32974.
NextBioi781313.
PROiP12646.

Gene expression databases

BgeeiP12646.
ExpressionAtlasiP12646. differential.
GenevisibleiP12646. DM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene."
    Fouts D., Ganguly R., Gutierrez A.G., Lucchesi J.C., Manning J.E.
    Gene 63:261-275(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
  2. "Historical selection, amino acid polymorphism and lineage-specific divergence at the G6pd locus in Drosophila melanogaster and D. simulans."
    Eanes W.F., Kirchner M., Yoon J., Biermann C.H., Wang I.N., McCartney M.A., Verrelli B.C.
    Genetics 144:1027-1041(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
    Strain: F23.3, F24.1, MT32, MT41, MT68, Z11, Z16, Z21, Z27, Z3, Z41, Z42, Z5, Z55, Z62, Z64 and Z74.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiG6PD_DROME
AccessioniPrimary (citable) accession number: P12646
Secondary accession number(s): Q27574
, Q27872, Q27879, Q27881, Q9VWE2, Q9VWE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: June 24, 2015
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.