P12646 (G6PD_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate 1-dehydrogenase
Short name=G6PD
EC=1.1.1.49

Alternative name(s):
Zwischenferment

Gene names

Name:	Zw
Synonyms:	G6PD
ORF Names:	CG12529

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from mutant phenotype PubMed 18809674. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P12646-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P12646-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Glucose-6-phosphate 1-dehydrogenase		PRO_0000068092
Sites
	Active site	267	1	Proton acceptor By similarity
	Binding site	44	1	NADP By similarity
	Binding site	76	1	NADP By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
Amino acid modifications
	Modified residue	20	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 22	22	Missing in isoform B.		VSP_001593
	Natural variant	32	1	G → C in strain: F24.1, MT32 and MT68.
	Natural variant	80	1	T → N in strain: Z74.
	Natural variant	382	1	L → P in strain: F23.3, MT41, Z3, Z5, Z11, Z16, Z21, Z27, Z42, Z55, Z64, Z74 and Berkeley.
Experimental info
	Sequence conflict	185 – 186	2	QA → AG in AAA51463. Ref.1
	Sequence conflict	214	1	N → K in AAA51463. Ref.1
	Sequence conflict	344 – 346	3	LGV → ARS in AAA51463. Ref.1
	Sequence conflict	461 – 465	5	DELRE → AAAQ in AAA51463. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified July 15, 1998. Version 2. Checksum: ABF81B763A82F1FD	FASTA	524	60,431
	10 20 30 40 50 60 MATQKEDHTA LDLIIKSLKS PTMVCEGTHF DGKIPHTFVI FGASGDLAKK KIYPTLWWLY 70 80 90 100 110 120 RDDLLPKPTK FCGYARSMLT VDSIKEQCLP YMKVQPHEQK KYEEFWALNE YVSGRYDGRT 130 140 150 160 170 180 GFELLNQQLE IMENKNKANR IFYLALPPSV FEEVTVNIKQ ICMSVCGWNR VIIEKPFGRD 190 200 210 220 230 240 DASSQALSDH LAGLFQEDQL YRIDHYLGKE MVQNLMTIRF GNKILSSTWN RENIASVLIT 250 260 270 280 290 300 FKEPFGTQGR GGYFDEFGII RDVMQNHLLQ ILSLVAMEKP VSCHPDDIRD EKVKVLKSIE 310 320 330 340 350 360 ALTLDDMVLG QYLGNPQGTN DDARTGYVED PTVSNDSNTP TYALGVLKIN NERWQGVPFI 370 380 390 400 410 420 LRCGKALNER KAEVRIQYQD VLGDIFEGNT KRNELVIRVQ PGEALYFKMM TKSPGITFDI 430 440 450 460 470 480 EETELDLTYE HRYKDSYLPD AYERLILDVF CGSQMHFVRS DELREAWRIF TPILHQIEKE 490 500 510 520 HIRPITYQYG SRGPKEADRK CEENNFKYSG SYKWHGGKAA TSNH
	Isoform B [UniParc]. Checksum: DFF55A83782BEDAC	FASTA	502	58,008
	10 20 30 40 50 60 MVCEGTHFDG KIPHTFVIFG ASGDLAKKKI YPTLWWLYRD DLLPKPTKFC GYARSMLTVD 70 80 90 100 110 120 SIKEQCLPYM KVQPHEQKKY EEFWALNEYV SGRYDGRTGF ELLNQQLEIM ENKNKANRIF 130 140 150 160 170 180 YLALPPSVFE EVTVNIKQIC MSVCGWNRVI IEKPFGRDDA SSQALSDHLA GLFQEDQLYR 190 200 210 220 230 240 IDHYLGKEMV QNLMTIRFGN KILSSTWNRE NIASVLITFK EPFGTQGRGG YFDEFGIIRD 250 260 270 280 290 300 VMQNHLLQIL SLVAMEKPVS CHPDDIRDEK VKVLKSIEAL TLDDMVLGQY LGNPQGTNDD 310 320 330 340 350 360 ARTGYVEDPT VSNDSNTPTY ALGVLKINNE RWQGVPFILR CGKALNERKA EVRIQYQDVL 370 380 390 400 410 420 GDIFEGNTKR NELVIRVQPG EALYFKMMTK SPGITFDIEE TELDLTYEHR YKDSYLPDAY 430 440 450 460 470 480 ERLILDVFCG SQMHFVRSDE LREAWRIFTP ILHQIEKEHI RPITYQYGSR GPKEADRKCE 490 500 ENNFKYSGSY KWHGGKAATS NH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene." Fouts D., Ganguly R., Gutierrez A.G., Lucchesi J.C., Manning J.E. Gene 63:261-275(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A).
[2]	"Historical selection, amino acid polymorphism and lineage-specific divergence at the G6pd locus in Drosophila melanogaster and D. simulans." Eanes W.F., Kirchner M., Yoon J., Biermann C.H., Wang I.N., McCartney M.A., Verrelli B.C. Genetics 144:1027-1041(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM A). Strain: F23.3, F24.1, MT32, MT41, MT68, Z11, Z16, Z21, Z27, Z3, Z41, Z42, Z5, Z55, Z62, Z64 and Z74.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26674, M26673 Genomic DNA. Translation: AAA51463.1. U42738 Genomic DNA. Translation: AAB02801.1. U42739 Genomic DNA. Translation: AAB02802.1. U42740 Genomic DNA. Translation: AAB02803.1. U42741 Genomic DNA. Translation: AAB02804.1. U42742 Genomic DNA. Translation: AAB02805.1. U42743 Genomic DNA. Translation: AAB02806.1. U42744 Genomic DNA. Translation: AAB02807.1. U42745 Genomic DNA. Translation: AAB02808.1. U42746 Genomic DNA. Translation: AAB02809.1. U42747 Genomic DNA. Translation: AAB02810.1. U42748 Genomic DNA. Translation: AAB02811.1. U42749 Genomic DNA. Translation: AAB02812.1. U43165 Genomic DNA. Translation: AAA99071.1. U43166 Genomic DNA. Translation: AAA99072.1. U43167 Genomic DNA. Translation: AAA99073.1. U44721 Genomic DNA. Translation: AAA99092.1. U45985 Genomic DNA. Translation: AAA99107.1. AE014298 Genomic DNA. Translation: AAF48999.1. AE014298 Genomic DNA. Translation: AAF49000.2. AY052079 mRNA. Translation: AAK93503.1.
PIR	A47740. DEFFG6. JT0272.
RefSeq	NP_523411.1. NM_078687.1. NP_728287.1. NM_167676.1.
UniGene	Dm.225.
3D structure databases
ProteinModelPortal	P12646.
SMR	P12646. Positions 36-514.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-20748N.
IntAct	P12646. 2 interactions.
MINT	MINT-882769.
Proteomic databases
PaxDb	P12646.
PRIDE	P12646.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	32974.
KEGG	dme:Dmel_CG12529.
Organism-specific databases
CTD	32974.
FlyBase	FBgn0004057. Zw.
Phylogenomic databases
eggNOG	COG0364.
InParanoid	P12646.
KO	K00036.
OrthoDB	EOG4CJSZ7.
Enzyme and pathway databases
UniPathway	UPA00115; UER00408.
Gene expression databases
Bgee	P12646.
GermOnline	CG12529. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR001282. G6P_DH. IPR019796. G6P_DH_AS. IPR022675. G6P_DH_C. IPR022674. G6P_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23429. PTHR23429. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	32974.
NextBio	781313.

Entry information

Entry name

G6PD_DROME

Accession

Primary (citable) accession number: P12646
Secondary accession number(s): Q27574 , Q27872, Q27879, Q27881, Q9VWE2, Q9VWE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project