P12636 (MBHL_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uptake hydrogenase large subunit
EC=1.12.99.6

Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase large subunit

Gene names

Name:	hupB
Synonyms:	hupL
Ordered Locus Names:	bll6941

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Uptake hydrogenase large subunit		PRO_0000199710
Sites
	Metal binding	75	1	Nickel Potential
	Metal binding	78	1	Nickel Potential
	Metal binding	575	1	Nickel Potential
	Metal binding	578	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P12636 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 0409EE8972F036B7	FASTA	596	65,923
	10 20 30 40 50 60 MGIQTPNGFN LDNSGKRIVV DPVTRIEGHM RVEVNVDADN VIRNAVSTGT MWRGIEVILK 70 80 90 100 110 120 NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPE NANSIRNLMQ LALQVHDHVV 130 140 150 160 170 180 HFYHLHALDW VDVVSALSAD PRATSTLAQS ISNWPLSSPG YFKDLQTRLK KFVESGQLGP 190 200 210 220 230 240 FKNGYWGSKA YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI 250 260 270 280 290 300 NVDGTGAVGA INMERLNLIS SIIDRLIEFN EMVYLPDVAA IGSFYKDWLY GGGLSGQSVL 310 320 330 340 350 360 AYGDVPEHAN DYSAKSLKLP RGAIINGNLS EVFPVDHANP DEIQEFVVHS WYKYPDETKG 370 380 390 400 410 420 LHPWDGVTEP NYVLGPNAKG TKTAIEQLDE GGKYSWIKAP RWKGHAMEVG PLARWVVGYA 430 440 450 460 470 480 QNKSEFKDPV DKFLRDLNLP TSALFSTLGR TAARALESVW AGRQMRYFQD KLVANIKAGD 490 500 510 520 530 540 SSTANVDKWK PESWPKEAKG VGFTEAPRGA LAHWIKIKDT KIDNYQCVVP TTWNGSPRDP 550 560 570 580 590 KGNIGAFEAS LMNTPMVNPE QPLEILRTIH SFDPCLACST HVMSPDGQEL AKVKVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase." Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O. Proc. Natl. Acad. Sci. U.S.A. 85:8395-8399(1988) [PubMed: 3054886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.
[3]	"Sequence and characterization of three genes within the hydrogenase gene cluster of Bradyrhizobium japonicum." Fu C., Maier R.J. Gene 141:47-52(1994) [PubMed: 8163174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 589-596.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04114 Genomic DNA. Translation: AAA26219.1. BA000040 Genomic DNA. Translation: BAC52206.1. L24446 Genomic DNA. Translation: AAD13471.1.
PIR	HQZJUL. B31341.
RefSeq	NP_773581.1. NC_004463.1.
3D structure databases
ProteinModelPortal	P12636.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1048593.
GenomeReviews	Gene locus bll6941 in contig BA000040_GR.
KEGG	bja:bll6941.
NMPDR	fig|224911.1.peg.6941.
PATRIC	21197757. VBIBraJap65052_7112.
Phylogenomic databases
HOGENOM	HBG733231.
OMA	DGVTEPN.
PhylomeDB	P12636.
ProtClustDB	CLSK820329.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6941-MONOMER.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
KO	K06281.
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_BRAJA

Accession

Primary (citable) accession number: P12636

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program