Reviewed,
UniProtKB/Swiss-Prot P12636 (MBHL_BRAJA)
Last modified
January 19, 2010.
Version 70.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Uptake hydrogenase large subunit EC=1.12.99.6 Alternative name(s): Hydrogenlyase Membrane-bound hydrogenase large subunit | ||||||
| Gene names |
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| Organism | Bradyrhizobium japonicum [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 375 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium |
Protein attributes
| Sequence length | 596 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 nickel ion per subunit By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Ligand | Metal-binding Nickel |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activityInferred from electronic annotation. Source: EC nickel ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase." Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O. Proc. Natl. Acad. Sci. U.S.A. 85:8395-8399(1988) [PubMed: 3054886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110. |
| [3] | "Sequence and characterization of three genes within the hydrogenase gene cluster of Bradyrhizobium japonicum." Fu C., Maier R.J. Gene 141:47-52(1994) [PubMed: 8163174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 589-596. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J04114 Genomic DNA. Translation: AAA26219.1. BA000040 Genomic DNA. Translation: BAC52206.1. L24446 Genomic DNA. Translation: AAD13471.1. |
| PIR | HQZJUL. B31341. |
| RefSeq | NP_773581.1. |
3D structure databases | |
| SMR | P12636. Positions 15-581. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1048593. |
| GenomeReviews | Gene locus bll6941 in contig BA000040_GR. |
| KEGG | bja:bll6941. |
| NMPDR | fig|224911.1.peg.6941. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG733231. |
| OMA | DGVTEPN. |
| PhylomeDB | P12636. |
Enzyme and pathway databases | |
| BioCyc | BJAP224911:BLL6941-MONOMER. |
| BRENDA | 1.12.99.6. 280. |
Family and domain databases | |
| InterPro | IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view] |
| Pfam | PF00374. NiFeSe_Hases. 1 hit. [Graphical view] |
| PROSITE | PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHL_BRAJA | ||||||||
| Accession | Primary (citable) accession number: P12636 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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