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Reviewed, UniProtKB/Swiss-Prot P12635 (MBHS_BRAJA)

Last modified November 3, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit
Gene names
Name: hupA
Synonyms: hupS
Ordered Locus Names: bll6942
OrganismBradyrhizobium japonicum [Complete proteome] [HAMAP]
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.

Catalytic activity

H2 + A = AH2.

Cofactor

Binds 1 3Fe-4S cluster By similarity.

Binds 2 4Fe-4S clusters By similarity.

Subunit structure

Heterodimer of a large and a small subunit.

Subcellular location

Cell membrane; Peripheral membrane protein.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Sequence similarities

Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4646Tat-type signal Potential
Chain47 – 363317Uptake hydrogenase small subunit
PRO_0000013425

Sites

Metal binding631Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding661Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1611Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1951Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding2331Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal binding2361Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2611Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2671Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2761Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2951Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2981Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P12635-1 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: E51DED25AE20D86A

FASTA36339,446
        10         20         30         40         50         60 
MGAATETFYS VIRRQGITRR SFHKFCSLTA TSLGLGPLAA SRIANALETK PRVPVIWMHG 

        70         80         90        100        110        120 
LECTCCSESF IRSAHPLVKD AVLSMISLDY DDTIMAAAGH QAEAILEETR AKHKGQYILA 

       130        140        150        160        170        180 
VEGNPPLNEG GMFCIDGGKP FVEKLKMMAE DAMAIIAWGA CASWGCVQAA KPNPTQATPI 

       190        200        210        220        230        240 
DKVITNKPII KVPGCPPIAE VMTGVVTFIT TFGKLPELDR QGRPKMFYSQ RIHDKCYRRP 

       250        260        270        280        290        300 
HFDAGQFVEE WDDEAARKGY CLYKMGCKGP TTYNACSTVR WNGGVSFPIQ SGHGCIGCSE 

       310        320        330        340        350        360 
DGFWDKGSFY DRLTNIKQFG IEKNADQIGM VAAGAVGAAV AAHAAVTAVK RLATKREDAD 


HNS 

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase."
Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O.
Proc. Natl. Acad. Sci. U.S.A. 85:8395-8399(1988) [PubMed: 3054886] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

J04114 Genomic DNA. Translation: AAA26218.1.
BA000040 Genomic DNA. Translation: BAC52207.1.
PIRHQZJUS. A31341.
RefSeqNP_773582.1.

3D structure databases

HSSPHSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBaseSearch...

Genome annotation databases

GeneID1048581.
GenomeReviewsGene locus bll6942 in contig BA000040_GR.
KEGGbja:bll6942.
NMPDRfig|224911.1.peg.6942.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP12635.
OMALEYHETV.

Enzyme and pathway databases

BioCycBJAP224911:BLL6942-MON.
BRENDA1.12.99.6. 280.

Family and domain databases

InterProIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR001821. NiFe_hydrogenase_ssu.
IPR006311. TAT_signal.
IPR017909. Twin_arg_translocation_Tat.
[Graphical view]
PfamPF01058. Oxidored_q6. 1 hit.
[Graphical view]
PIRSFPIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTSPR00614. NIHGNASESMLL.
TIGRFAMsTIGR00391. hydA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMBHS_BRAJA
AccessionPrimary (citable) accession number: P12635
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents