Uptake hydrogenase small subunit precursor

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Reviewed, UniProtKB/Swiss-Prot P12635 (MBHS_BRAJA)

Last modified November 3, 2009. Version 77. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:	hupA
Synonyms:	hupS
Ordered Locus Names:	bll6942

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

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Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	This enzyme recycles the H₂ produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O₂ under carbon- or phosphate-limited conditions.
Catalytic activity	H₂ + A = AH₂.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 46

Tat-type signal Potential

Chain

47 – 363

317

Uptake hydrogenase small subunit

PRO_0000013425

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

161

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

195

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

233

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

236

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

261

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

267

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

276

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

295

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

298

Iron-sulfur 3 (3Fe-4S) By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P12635-1 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: E51DED25AE20D86A
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FASTA

363

39,446

        10         20         30         40         50         60 
MGAATETFYS VIRRQGITRR SFHKFCSLTA TSLGLGPLAA SRIANALETK PRVPVIWMHG 

        70         80         90        100        110        120 
LECTCCSESF IRSAHPLVKD AVLSMISLDY DDTIMAAAGH QAEAILEETR AKHKGQYILA 

       130        140        150        160        170        180 
VEGNPPLNEG GMFCIDGGKP FVEKLKMMAE DAMAIIAWGA CASWGCVQAA KPNPTQATPI 

       190        200        210        220        230        240 
DKVITNKPII KVPGCPPIAE VMTGVVTFIT TFGKLPELDR QGRPKMFYSQ RIHDKCYRRP 

       250        260        270        280        290        300 
HFDAGQFVEE WDDEAARKGY CLYKMGCKGP TTYNACSTVR WNGGVSFPIQ SGHGCIGCSE 

       310        320        330        340        350        360 
DGFWDKGSFY DRLTNIKQFG IEKNADQIGM VAAGAVGAAV AAHAAVTAVK RLATKREDAD 


HNS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase." Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O. Proc. Natl. Acad. Sci. U.S.A. 85:8395-8399(1988) [PubMed: 3054886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

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Cross-references

Sequence databases
	J04114 Genomic DNA. Translation: AAA26218.1. BA000040 Genomic DNA. Translation: BAC52207.1.
PIR	HQZJUS. A31341.
RefSeq	NP_773582.1.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Genome annotation databases
GeneID	1048581.
GenomeReviews	Gene locus bll6942 in contig BA000040_GR.
KEGG	bja:bll6942.
NMPDR	fig\|224911.1.peg.6942.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P12635.
OMA	LEYHETV.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL6942-MON.
BRENDA	1.12.99.6. 280.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MBHS_BRAJA

Accession

Primary (citable) accession number: P12635

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents