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P12630 (BAR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Barrierpepsin

EC=3.4.23.35
Alternative name(s):
BAR proteinase
Extracellular 'barrier' protein
Gene names
Name:BAR1
Synonyms:SST1
Ordered Locus Names:YIL015W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein called "barrier activity" is excreted by yeast cells mating type a. It is probably a protease that cleaves alpha-factor and thus acts as an antagonist of this mating pheromone and establishes optimal pheromone concentration for conjugation.

Catalytic activity

Selective cleavage of 6-Leu-|-Lys-7 bond in the pheromone alpha-mating factor.

Subcellular location

Secreted.

Induction

By alpha factor.

Miscellaneous

It is found only in a mating type cells.

Present with 672 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase A1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 587563Barrierpepsin
PRO_0000025834

Sites

Active site631 By similarity
Active site2871 By similarity

Amino acid modifications

Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation3081N-linked (GlcNAc...) Potential
Glycosylation3661N-linked (GlcNAc...) Potential
Glycosylation3981N-linked (GlcNAc...) Potential
Glycosylation4681N-linked (GlcNAc...) Potential
Glycosylation5031N-linked (GlcNAc...) Potential
Glycosylation5511N-linked (GlcNAc...) Potential
Disulfide bond322 ↔ 358 By similarity

Sequences

Sequence LengthMass (Da)Tools
P12630 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: CC21DB7FDBC83984

FASTA58763,730
        10         20         30         40         50         60 
MSAINHLCLK LILASFAIIN TITALTNDGT GHLEFLLQHE EEMYYATTLD IGTPSQSLTV 

        70         80         90        100        110        120 
LFDTGSADFW VMDSSNPFCL PNSNTSSYSN ATYNGEEVKP SIDCRSMSTY NEHRSSTYQY 

       130        140        150        160        170        180 
LENGRFYITY ADGTFADGSW GTETVSINGI DIPNIQFGVA KYATTPVSGV LGIGFPRRES 

       190        200        210        220        230        240 
VKGYEGAPNE YYPNFPQILK SEKIIDVVAY SLFLNSPDSG TGSIVFGAID ESKFSGDLFT 

       250        260        270        280        290        300 
FPMVNEYPTI VDAPATLAMT IQGLGAQNKS SCEHETFTTT KYPVLLDSGT SLLNAPKVIA 

       310        320        330        340        350        360 
DKMASFVNAS YSEEEGIYIL DCPVSVGDVE YNFDFGDLQI SVPLSSLILS PETEGSYCGF 

       370        380        390        400        410        420 
AVQPTNDSMV LGDVFLSSAY VVFDLDNYKI SLAQANWNAS EVSKKLVNIQ TDGSISGAKI 

       430        440        450        460        470        480 
ATAEPWSTNE PFTVTSDIYS STGCKSRPFL QSSTASSLIA ETNVQSRNCS TKMPGTRSTT 

       490        500        510        520        530        540 
VLSKPTQNSA MHQSTGAVTQ TSNETKLELS STMANSGSVS LPTSNSIDKE FEHSKSQTTS 

       550        560        570        580 
DPSVAEHSTF NQTFVHETKY RPTHKTVITE TVTKYSTVLI NVCKPTY 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin."
Mackay V.L., Welch S.K., Insley M.Y., Manney T.R., Holly J., Saari G.C., Parker M.L.
Proc. Natl. Acad. Sci. U.S.A. 85:55-59(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46881 Genomic DNA. Translation: CAA86977.1.
J03573 Genomic DNA. Translation: AAA34451.1.
BK006942 Genomic DNA. Translation: DAA08530.1.
PIRA34084.
RefSeqNP_012249.1. NM_001179365.1.

3D structure databases

ProteinModelPortalP12630.
SMRP12630. Positions 45-400.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34973. 16 interactions.
IntActP12630. 1 interaction.
MINTMINT-2782575.
STRING4932.YIL015W.

Protein family/group databases

MEROPSA01.015.

Proteomic databases

MaxQBP12630.
PaxDbP12630.
PeptideAtlasP12630.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL015W; YIL015W; YIL015W.
GeneID854797.
KEGGsce:YIL015W.

Organism-specific databases

CYGDYIL015w.
SGDS000001277. BAR1.

Phylogenomic databases

eggNOGNOG248628.
GeneTreeENSGT00550000075429.
HOGENOMHOG000074716.
KOK01383.
OMAIINTITA.
OrthoDBEOG71P2M6.

Enzyme and pathway databases

BioCycYEAST:G3O-31291-MONOMER.

Gene expression databases

GenevestigatorP12630.

Family and domain databases

Gene3D2.40.70.10. 2 hits.
InterProIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERPTHR13683. PTHR13683. 1 hit.
PfamPF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
SUPFAMSSF50630. SSF50630. 1 hit.
PROSITEPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio977603.

Entry information

Entry nameBAR1_YEAST
AccessionPrimary (citable) accession number: P12630
Secondary accession number(s): D6VVR4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 14, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries