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P12630

- BAR1_YEAST

UniProt

P12630 - BAR1_YEAST

Protein

Barrierpepsin

Gene

BAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    This protein called "barrier activity" is excreted by yeast cells mating type a. It is probably a protease that cleaves alpha-factor and thus acts as an antagonist of this mating pheromone and establishes optimal pheromone concentration for conjugation.

    Catalytic activityi

    Selective cleavage of 6-Leu-|-Lys-7 bond in the pheromone alpha-mating factor.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631PROSITE-ProRule annotation
    Active sitei287 – 2871PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: SGD

    GO - Biological processi

    1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
    2. peptide catabolic process Source: SGD

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Keywords - Biological processi

    Pheromone response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31291-MONOMER.

    Protein family/group databases

    MEROPSiA01.015.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Barrierpepsin (EC:3.4.23.35)
    Alternative name(s):
    BAR proteinase
    Extracellular 'barrier' protein
    Gene namesi
    Name:BAR1
    Synonyms:SST1
    Ordered Locus Names:YIL015W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL015w.
    SGDiS000001277. BAR1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall-bounded periplasmic space Source: SGD
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Add
    BLAST
    Chaini25 – 587563BarrierpepsinPRO_0000025834Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi322 ↔ 358By similarity
    Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi468 – 4681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP12630.
    PaxDbiP12630.
    PeptideAtlasiP12630.

    Expressioni

    Inductioni

    By alpha factor.

    Gene expression databases

    GenevestigatoriP12630.

    Interactioni

    Protein-protein interaction databases

    BioGridi34973. 16 interactions.
    IntActiP12630. 1 interaction.
    MINTiMINT-2782575.
    STRINGi4932.YIL015W.

    Structurei

    3D structure databases

    ProteinModelPortaliP12630.
    SMRiP12630. Positions 45-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248628.
    GeneTreeiENSGT00550000075429.
    HOGENOMiHOG000074716.
    KOiK01383.
    OMAiIINTITA.
    OrthoDBiEOG71P2M6.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12630-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAINHLCLK LILASFAIIN TITALTNDGT GHLEFLLQHE EEMYYATTLD    50
    IGTPSQSLTV LFDTGSADFW VMDSSNPFCL PNSNTSSYSN ATYNGEEVKP 100
    SIDCRSMSTY NEHRSSTYQY LENGRFYITY ADGTFADGSW GTETVSINGI 150
    DIPNIQFGVA KYATTPVSGV LGIGFPRRES VKGYEGAPNE YYPNFPQILK 200
    SEKIIDVVAY SLFLNSPDSG TGSIVFGAID ESKFSGDLFT FPMVNEYPTI 250
    VDAPATLAMT IQGLGAQNKS SCEHETFTTT KYPVLLDSGT SLLNAPKVIA 300
    DKMASFVNAS YSEEEGIYIL DCPVSVGDVE YNFDFGDLQI SVPLSSLILS 350
    PETEGSYCGF AVQPTNDSMV LGDVFLSSAY VVFDLDNYKI SLAQANWNAS 400
    EVSKKLVNIQ TDGSISGAKI ATAEPWSTNE PFTVTSDIYS STGCKSRPFL 450
    QSSTASSLIA ETNVQSRNCS TKMPGTRSTT VLSKPTQNSA MHQSTGAVTQ 500
    TSNETKLELS STMANSGSVS LPTSNSIDKE FEHSKSQTTS DPSVAEHSTF 550
    NQTFVHETKY RPTHKTVITE TVTKYSTVLI NVCKPTY 587
    Length:587
    Mass (Da):63,730
    Last modified:October 1, 1989 - v1
    Checksum:iCC21DB7FDBC83984
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46881 Genomic DNA. Translation: CAA86977.1.
    J03573 Genomic DNA. Translation: AAA34451.1.
    BK006942 Genomic DNA. Translation: DAA08530.1.
    PIRiA34084.
    RefSeqiNP_012249.1. NM_001179365.1.

    Genome annotation databases

    EnsemblFungiiYIL015W; YIL015W; YIL015W.
    GeneIDi854797.
    KEGGisce:YIL015W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46881 Genomic DNA. Translation: CAA86977.1 .
    J03573 Genomic DNA. Translation: AAA34451.1 .
    BK006942 Genomic DNA. Translation: DAA08530.1 .
    PIRi A34084.
    RefSeqi NP_012249.1. NM_001179365.1.

    3D structure databases

    ProteinModelPortali P12630.
    SMRi P12630. Positions 45-400.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34973. 16 interactions.
    IntActi P12630. 1 interaction.
    MINTi MINT-2782575.
    STRINGi 4932.YIL015W.

    Protein family/group databases

    MEROPSi A01.015.

    Proteomic databases

    MaxQBi P12630.
    PaxDbi P12630.
    PeptideAtlasi P12630.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL015W ; YIL015W ; YIL015W .
    GeneIDi 854797.
    KEGGi sce:YIL015W.

    Organism-specific databases

    CYGDi YIL015w.
    SGDi S000001277. BAR1.

    Phylogenomic databases

    eggNOGi NOG248628.
    GeneTreei ENSGT00550000075429.
    HOGENOMi HOG000074716.
    KOi K01383.
    OMAi IINTITA.
    OrthoDBi EOG71P2M6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31291-MONOMER.

    Miscellaneous databases

    NextBioi 977603.

    Gene expression databases

    Genevestigatori P12630.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin."
      Mackay V.L., Welch S.K., Insley M.Y., Manney T.R., Holly J., Saari G.C., Parker M.L.
      Proc. Natl. Acad. Sci. U.S.A. 85:55-59(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBAR1_YEAST
    AccessioniPrimary (citable) accession number: P12630
    Secondary accession number(s): D6VVR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It is found only in a mating type cells.
    Present with 672 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3