P12630 (BAR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Barrierpepsin
EC=3.4.23.35

Alternative name(s):
BAR proteinase
Extracellular 'barrier' protein

Gene names

Name:	BAR1
Synonyms:	SST1
Ordered Locus Names:	YIL015W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein called "barrier activity" is excreted by yeast cells mating type a. It is probably a protease that cleaves alpha-factor and thus acts as an antagonist of this mating pheromone and establishes optimal pheromone concentration for conjugation.
Catalytic activity	Selective cleavage of 6-Leu-|-Lys-7 bond in the pheromone alpha-mating factor.
Subcellular location	Secreted.
Induction	By alpha factor.
Miscellaneous	It is found only in a mating type cells. Present with 672 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Inferred from direct assay PubMed 6345506. Source: SGD peptide catabolic process Inferred from direct assay PubMed 9249020. Source: SGD proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall-bounded periplasmic space Traceable author statement PubMed 9249020. Source: SGD extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from direct assay PubMed 9249020. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 587	563	Barrierpepsin		PRO_0000025834
Sites
	Active site	63	1	By similarity
	Active site	287	1	By similarity
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential
	Glycosylation	503	1	N-linked (GlcNAc...) Potential
	Glycosylation	551	1	N-linked (GlcNAc...) Potential
	Disulfide bond	322 ↔ 358		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12630 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: CC21DB7FDBC83984	FASTA	587	63,730
	10 20 30 40 50 60 MSAINHLCLK LILASFAIIN TITALTNDGT GHLEFLLQHE EEMYYATTLD IGTPSQSLTV 70 80 90 100 110 120 LFDTGSADFW VMDSSNPFCL PNSNTSSYSN ATYNGEEVKP SIDCRSMSTY NEHRSSTYQY 130 140 150 160 170 180 LENGRFYITY ADGTFADGSW GTETVSINGI DIPNIQFGVA KYATTPVSGV LGIGFPRRES 190 200 210 220 230 240 VKGYEGAPNE YYPNFPQILK SEKIIDVVAY SLFLNSPDSG TGSIVFGAID ESKFSGDLFT 250 260 270 280 290 300 FPMVNEYPTI VDAPATLAMT IQGLGAQNKS SCEHETFTTT KYPVLLDSGT SLLNAPKVIA 310 320 330 340 350 360 DKMASFVNAS YSEEEGIYIL DCPVSVGDVE YNFDFGDLQI SVPLSSLILS PETEGSYCGF 370 380 390 400 410 420 AVQPTNDSMV LGDVFLSSAY VVFDLDNYKI SLAQANWNAS EVSKKLVNIQ TDGSISGAKI 430 440 450 460 470 480 ATAEPWSTNE PFTVTSDIYS STGCKSRPFL QSSTASSLIA ETNVQSRNCS TKMPGTRSTT 490 500 510 520 530 540 VLSKPTQNSA MHQSTGAVTQ TSNETKLELS STMANSGSVS LPTSNSIDKE FEHSKSQTTS 550 560 570 580 DPSVAEHSTF NQTFVHETKY RPTHKTVITE TVTKYSTVLI NVCKPTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin." Mackay V.L., Welch S.K., Insley M.Y., Manney T.R., Holly J., Saari G.C., Parker M.L. Proc. Natl. Acad. Sci. U.S.A. 85:55-59(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46881 Genomic DNA. Translation: CAA86977.1. J03573 Genomic DNA. Translation: AAA34451.1. BK006942 Genomic DNA. Translation: DAA08530.1.
PIR	A34084.
RefSeq	NP_012249.1. NM_001179365.1.
3D structure databases
ProteinModelPortal	P12630.
SMR	P12630. Positions 45-400.
ModBase	Search...
Protein-protein interaction databases
IntAct	P12630. 1 interaction.
MINT	MINT-2782575.
STRING	4932.YIL015W.
Protein family/group databases
MEROPS	A01.015.
Proteomic databases
PaxDb	P12630.
PeptideAtlas	P12630.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL015W; YIL015W; YIL015W.
GeneID	854797.
KEGG	sce:YIL015W.
Organism-specific databases
CYGD	YIL015w.
SGD	S000001277. BAR1.
Phylogenomic databases
eggNOG	NOG248628.
GeneTree	ENSGT00550000075429.
HOGENOM	HOG000074716.
KO	K01383.
OMA	VFDLDNY.
OrthoDB	EOG4J6W11.
Gene expression databases
Genevestigator	P12630.
GermOnline	YIL015W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	977603.

Entry information

Entry name

BAR1_YEAST

Accession

Primary (citable) accession number: P12630
Secondary accession number(s): D6VVR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program