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Protein

Barrierpepsin

Gene

BAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein called "barrier activity" is excreted by yeast cells mating type a. It is probably a protease that cleaves alpha-factor and thus acts as an antagonist of this mating pheromone and establishes optimal pheromone concentration for conjugation.

Miscellaneous

It is found only in a mating type cells.
Present with 672 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Selective cleavage of 6-Leu-|-Lys-7 bond in the pheromone alpha-mating factor.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63PROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processPheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-31291-MONOMER

Protein family/group databases

MEROPSiA01.015

Names & Taxonomyi

Protein namesi
Recommended name:
Barrierpepsin (EC:3.4.23.35)
Alternative name(s):
BAR proteinase
Extracellular 'barrier' protein
Gene namesi
Name:BAR1
Synonyms:SST1
Ordered Locus Names:YIL015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL015W
SGDiS000001277 BAR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000002583425 – 587BarrierpepsinAdd BLAST563

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi322 ↔ 358By similarity
Glycosylationi366N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi398N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi468N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi503N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi551N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP12630
PaxDbiP12630
PRIDEiP12630

Expressioni

Inductioni

By alpha factor.

Interactioni

Protein-protein interaction databases

BioGridi34973, 87 interactors
IntActiP12630, 1 interactor
MINTiP12630
STRINGi4932.YIL015W

Structurei

3D structure databases

ProteinModelPortaliP12630
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 393Peptidase A1PROSITE-ProRule annotationAdd BLAST349

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00550000075429
HOGENOMiHOG000074716
InParanoidiP12630
KOiK01383
OMAiMARANTN
OrthoDBiEOG092C3KPP

Family and domain databases

CDDicd05474 SAP_like, 1 hit
Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
IPR033876 SAP-like
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAINHLCLK LILASFAIIN TITALTNDGT GHLEFLLQHE EEMYYATTLD
60 70 80 90 100
IGTPSQSLTV LFDTGSADFW VMDSSNPFCL PNSNTSSYSN ATYNGEEVKP
110 120 130 140 150
SIDCRSMSTY NEHRSSTYQY LENGRFYITY ADGTFADGSW GTETVSINGI
160 170 180 190 200
DIPNIQFGVA KYATTPVSGV LGIGFPRRES VKGYEGAPNE YYPNFPQILK
210 220 230 240 250
SEKIIDVVAY SLFLNSPDSG TGSIVFGAID ESKFSGDLFT FPMVNEYPTI
260 270 280 290 300
VDAPATLAMT IQGLGAQNKS SCEHETFTTT KYPVLLDSGT SLLNAPKVIA
310 320 330 340 350
DKMASFVNAS YSEEEGIYIL DCPVSVGDVE YNFDFGDLQI SVPLSSLILS
360 370 380 390 400
PETEGSYCGF AVQPTNDSMV LGDVFLSSAY VVFDLDNYKI SLAQANWNAS
410 420 430 440 450
EVSKKLVNIQ TDGSISGAKI ATAEPWSTNE PFTVTSDIYS STGCKSRPFL
460 470 480 490 500
QSSTASSLIA ETNVQSRNCS TKMPGTRSTT VLSKPTQNSA MHQSTGAVTQ
510 520 530 540 550
TSNETKLELS STMANSGSVS LPTSNSIDKE FEHSKSQTTS DPSVAEHSTF
560 570 580
NQTFVHETKY RPTHKTVITE TVTKYSTVLI NVCKPTY
Length:587
Mass (Da):63,730
Last modified:October 1, 1989 - v1
Checksum:iCC21DB7FDBC83984
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA Translation: CAA86977.1
J03573 Genomic DNA Translation: AAA34451.1
BK006942 Genomic DNA Translation: DAA08530.1
PIRiA34084
RefSeqiNP_012249.1, NM_001179365.1

Genome annotation databases

EnsemblFungiiYIL015W; YIL015W; YIL015W
GeneIDi854797
KEGGisce:YIL015W

Similar proteinsi

Entry informationi

Entry nameiBAR1_YEAST
AccessioniPrimary (citable) accession number: P12630
Secondary accession number(s): D6VVR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names
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