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Protein

Barrierpepsin

Gene

BAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein called "barrier activity" is excreted by yeast cells mating type a. It is probably a protease that cleaves alpha-factor and thus acts as an antagonist of this mating pheromone and establishes optimal pheromone concentration for conjugation.

Catalytic activityi

Selective cleavage of 6-Leu-|-Lys-7 bond in the pheromone alpha-mating factor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631PROSITE-ProRule annotation
Active sitei287 – 2871PROSITE-ProRule annotation

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: SGD

GO - Biological processi

  • adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  • peptide catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-31291-MONOMER.

Protein family/group databases

MEROPSiA01.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Barrierpepsin (EC:3.4.23.35)
Alternative name(s):
BAR proteinase
Extracellular 'barrier' protein
Gene namesi
Name:BAR1
Synonyms:SST1
Ordered Locus Names:YIL015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIL015w.
EuPathDBiFungiDB:YIL015W.
SGDiS000001277. BAR1.

Subcellular locationi

GO - Cellular componenti

  • cell wall-bounded periplasmic space Source: SGD
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 587563BarrierpepsinPRO_0000025834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi322 ↔ 358By similarity
Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi468 – 4681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP12630.
PaxDbiP12630.
PeptideAtlasiP12630.

Expressioni

Inductioni

By alpha factor.

Interactioni

Protein-protein interaction databases

BioGridi34973. 19 interactions.
IntActiP12630. 1 interaction.
MINTiMINT-2782575.

Structurei

3D structure databases

ProteinModelPortaliP12630.
SMRiP12630. Positions 45-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248628.
GeneTreeiENSGT00550000075429.
HOGENOMiHOG000074716.
InParanoidiP12630.
KOiK01383.
OMAiMENNEIS.
OrthoDBiEOG71P2M6.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAINHLCLK LILASFAIIN TITALTNDGT GHLEFLLQHE EEMYYATTLD
60 70 80 90 100
IGTPSQSLTV LFDTGSADFW VMDSSNPFCL PNSNTSSYSN ATYNGEEVKP
110 120 130 140 150
SIDCRSMSTY NEHRSSTYQY LENGRFYITY ADGTFADGSW GTETVSINGI
160 170 180 190 200
DIPNIQFGVA KYATTPVSGV LGIGFPRRES VKGYEGAPNE YYPNFPQILK
210 220 230 240 250
SEKIIDVVAY SLFLNSPDSG TGSIVFGAID ESKFSGDLFT FPMVNEYPTI
260 270 280 290 300
VDAPATLAMT IQGLGAQNKS SCEHETFTTT KYPVLLDSGT SLLNAPKVIA
310 320 330 340 350
DKMASFVNAS YSEEEGIYIL DCPVSVGDVE YNFDFGDLQI SVPLSSLILS
360 370 380 390 400
PETEGSYCGF AVQPTNDSMV LGDVFLSSAY VVFDLDNYKI SLAQANWNAS
410 420 430 440 450
EVSKKLVNIQ TDGSISGAKI ATAEPWSTNE PFTVTSDIYS STGCKSRPFL
460 470 480 490 500
QSSTASSLIA ETNVQSRNCS TKMPGTRSTT VLSKPTQNSA MHQSTGAVTQ
510 520 530 540 550
TSNETKLELS STMANSGSVS LPTSNSIDKE FEHSKSQTTS DPSVAEHSTF
560 570 580
NQTFVHETKY RPTHKTVITE TVTKYSTVLI NVCKPTY
Length:587
Mass (Da):63,730
Last modified:October 1, 1989 - v1
Checksum:iCC21DB7FDBC83984
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA. Translation: CAA86977.1.
J03573 Genomic DNA. Translation: AAA34451.1.
BK006942 Genomic DNA. Translation: DAA08530.1.
PIRiA34084.
RefSeqiNP_012249.1. NM_001179365.1.

Genome annotation databases

EnsemblFungiiYIL015W; YIL015W; YIL015W.
GeneIDi854797.
KEGGisce:YIL015W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46881 Genomic DNA. Translation: CAA86977.1.
J03573 Genomic DNA. Translation: AAA34451.1.
BK006942 Genomic DNA. Translation: DAA08530.1.
PIRiA34084.
RefSeqiNP_012249.1. NM_001179365.1.

3D structure databases

ProteinModelPortaliP12630.
SMRiP12630. Positions 45-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34973. 19 interactions.
IntActiP12630. 1 interaction.
MINTiMINT-2782575.

Protein family/group databases

MEROPSiA01.015.

Proteomic databases

MaxQBiP12630.
PaxDbiP12630.
PeptideAtlasiP12630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL015W; YIL015W; YIL015W.
GeneIDi854797.
KEGGisce:YIL015W.

Organism-specific databases

CYGDiYIL015w.
EuPathDBiFungiDB:YIL015W.
SGDiS000001277. BAR1.

Phylogenomic databases

eggNOGiNOG248628.
GeneTreeiENSGT00550000075429.
HOGENOMiHOG000074716.
InParanoidiP12630.
KOiK01383.
OMAiMENNEIS.
OrthoDBiEOG71P2M6.

Enzyme and pathway databases

BioCyciYEAST:G3O-31291-MONOMER.

Miscellaneous databases

NextBioi977603.
PROiP12630.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin."
    Mackay V.L., Welch S.K., Insley M.Y., Manney T.R., Holly J., Saari G.C., Parker M.L.
    Proc. Natl. Acad. Sci. U.S.A. 85:55-59(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBAR1_YEAST
AccessioniPrimary (citable) accession number: P12630
Secondary accession number(s): D6VVR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 22, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

It is found only in a mating type cells.
Present with 672 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.