Reviewed, UniProtKB/Swiss-Prot P12628 (MAOX_PHAVU)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:

ME1

Organism

Phaseolus vulgaris (Kidney bean) (French bean)

Taxonomic identifier

3885 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmPotential.
Induction	By fungal elicitor.
Sequence similarities	Belongs to the malic enzymes family.
Caution	Was originally (Ref.1) thought to be a cinnamyl-alcohol dehydrogenase.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	NADP-dependent malic enzyme		PRO_0000160201
Regions
	Nucleotide binding	333 – 349	17	NADP By similarity
Sites
	Active site	137	1	Proton donor By similarity
	Active site	208	1	Proton acceptor By similarity
	Metal binding	280	1	Divalent metal cation By similarity
	Metal binding	281	1	Divalent metal cation By similarity
	Metal binding	304	1	Divalent metal cation By similarity
	Binding site	190	1	NAD By similarity
	Binding site	304	1	NAD By similarity
	Binding site	445	1	NAD By similarity
	Site	304	1	Important for activity By similarity
Experimental info
	Sequence conflict	16	1	K → N in CAA56354. Ref.2
	Sequence conflict	130	1	A → E in CAA56354. Ref.2
	Sequence conflict	258 – 259	2	TF → EL in CAA56354. Ref.2
	Sequence conflict	350	1	V → L in CAA56354. Ref.2
	Sequence conflict	567	1	K → P in CAA56354. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12628-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 9BFADDB2699BAC1E	FASTA	589	64,932
	10 20 30 40 50 60 MSSISLKENG GEVSVKKDYS NGGGVRDLYG EDSATEDHLI TPWTFSVASG CSLLRDPRYN 70 80 90 100 110 120 KGLAFTEGER DAHYLRGLLP PSVFNQELQE KRLMHNLRQY EVPLHRYMAL MDLQERNERL 130 140 150 160 170 180 FYKLLIDNVA ELLPVVYTPT VGEACQKYGS IFRRPQGLYI SLKEKGKILE VLKNWPEKSI 190 200 210 220 230 240 QVIVVTDGER ILGLGDLGCQ GMGIPVGKLS LYTALGGVRP SSCLPVTIDV GTNNEKLLND 250 260 270 280 290 300 EFYIGLRQRR ATGQEYATFL DEFMRAVKQN YGEKVLVQFE DFANHNAFDL LEKYSSSHLV 310 320 330 340 350 360 FNDDIQGTAS VVLAGLLASL KLVGGTLADH TFLFLGAGEA GTGIAELIAV EVSKQTKAPV 370 380 390 400 410 420 EETRKKIWLV DSKGLIVSSR LESLQQFKKP WAHEHEPVKG LLEAVKAIKP TVLIGSSGAG 430 440 450 460 470 480 KTFTKEVVET MASLNEKPLI LALSNPTSQS ECTAEEAYTW SKGRAIFASG SPFDPVEYEG 490 500 510 520 530 540 KLFVPGQANN AYIFPGFGLG LIMSGAIRVR DEMLLAASEA LAAQVSEENY DKGLIYPPFT 550 560 570 580 NIRKISANIA AKVAAKAYDL GLASHLKRPK DLVKYAESCM YSPGYRSYR

References

[1]	"Cinnamyl-alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor." Walter M.H., Grima-Pettenati J., Grand C., Boudet A.M., Lamb C.J. Proc. Natl. Acad. Sci. U.S.A. 85:5546-5550(1988) [PubMed: 3041415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of a bean (Phaseolus vulgaris L.) malic-enzyme gene." Walter M.H., Grima-Pettenati J., Feuillet C. Eur. J. Biochem. 224:999-1009(1994) [PubMed: 7925425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Processor. Tissue: Leaf.
[3]	"Extensive sequence similarity of the bean CAD4 (cinnamyl-alcohol dehydrogenase) to a maize malic enzyme." Walter M.H., Grima-Pettenati J., Grand C., Boudet A.M., Lamb C.J. Plant Mol. Biol. 15:525-526(1990) [PubMed: 2103472] [Abstract] Cited for: SIMILARITY TO MALIC ENZYMES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03825 mRNA. Translation: AAA19575.1. X80051 Genomic DNA. Translation: CAA56354.1.
PIR	DEFBC. S48198.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_PHAVU

Accession

Primary (citable) accession number: P12628

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)