P12614 (BGLS_AGRSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucosidase
EC=3.2.1.21

Alternative name(s):
Amygdalase
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase

Gene names

Name:

abg

Organism

Agrobacterium sp. (strain ATCC 21400)

Taxonomic identifier

74562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Beta-glucosidase		PRO_0000063869
Sites
	Active site	171	1	Proton donor Potential
	Active site	359	1	Nucleophile Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12614 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: AAB799153493A682	FASTA	459	51,170
	10 20 30 40 50 60 MTDPNTLAAR FPGDFLFGVA TASFQIEGST KADGRKPSIW DAFCNMPGHV FGRHNGDIAC 70 80 90 100 110 120 DHYNRWEEDL DLIKEMGVEA YRFSLAWPRI IPDGFGPINE KGLDFYDRLV DGCKARGIKT 130 140 150 160 170 180 YATLYHWDLP LTLMGDGGWA SRSTAHAFQR YAKTVMARLG DRLDAVATFN EPWCAVWLSH 190 200 210 220 230 240 LYGVHAPGER NMEAALAAMH HINLAHGFGV EASRHVAPKV PVGLVLNAHS AIPASDGEAD 250 260 270 280 290 300 LKAAERAFQF HNGAFFDPVF KGEYPAEMME ALGDRMPVVE AEDLGIISQK LDWWGLNYYT 310 320 330 340 350 360 PMRVADDATP GVEFPATMPA PAVSDVKTDI GWEVYAPALH TLVETLYERY DLPECYITEN 370 380 390 400 410 420 GACYNMGVEN GQVNDQPRLD YYAEHLGIVA DLIRDGYPMR GYFAWSLMDN FEWAEGYRMR 430 440 450 FGLVHVDYQT QVRTVKNSGK WYSALASGFP KGNHGVAKG

References

[1]	"Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400." Wakarchuk W.W., Greenberg N.M., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. J. Bacteriol. 170:301-307(1988) [PubMed: 2826395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Unequivocal demonstration of the involvement of a glutamate residue as a nucleophile in the mechanism of a 'retaining' glycosidase." Withers S.G., Warren R.A.J., Street I.P., Rupitz K., Kempton J.B., Aebersold R. J. Am. Chem. Soc. 112:5887-5889(1990) Cited for: ACTIVE SITE GLU-359.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19033 Genomic DNA. Translation: AAA22085.1.
PIR	GLAG. A28673.
3D structure databases
ProteinModelPortal	P12614.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017736. Glyco_hydro_1_beta-glucosidase. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR03356. BGL. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLS_AGRSA

Accession

Primary (citable) accession number: P12614

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 31, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program