P12611 (WHI2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Growth regulation protein

Gene names

Name:	WHI2
Ordered Locus Names:	YOR043W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the coordination of growth and proliferation. Required for entry into G0 phase under conditions of carbon limitation. Involved in the general stress response; acts together with PSR1 to activate stress response element (STRE)-mediated gene expression, possibly through dephosphorylation of MSN2. Ref.1 Ref.4
Subunit structure	Interacts with MSN2 and PSR1. Ref.4
Disruption phenotype	Negative mutants fail to enter the G0 phase under conditions of carbon limitation. Ref.1
Miscellaneous	Present with 1800 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WHI2 family.

Ontologies

Keywords
Biological process	Cell cycle
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from mutant phenotype PubMed 15020461. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW endocytosis Inferred from mutant phenotype PubMed 15020461. Source: SGD mitochondrion degradation Inferred from mutant phenotype PubMed 21429936. Source: SGD regulation of growth Inferred from mutant phenotype PubMed 2204680. Source: SGD response to heat Inferred from mutant phenotype Ref.4. Source: UniProtKB response to salt stress Inferred from mutant phenotype Ref.4. Source: UniProtKB
Molecular_function	phosphatase activator activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PSR1	Q07800	4	EBI-20530,EBI-31129

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Growth regulation protein		PRO_0000065967
Amino acid modifications
	Modified residue	130	1	Phosphoserine Ref.7
	Modified residue	131	1	Phosphoserine Ref.7 Ref.8
	Modified residue	380	1	Phosphoserine Ref.6
	Modified residue	386	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	216	1	F → L in AAA35218. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12611 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 105419D6180207F2	FASTA	486	55,346
	10 20 30 40 50 60 MDDIITQVSP DNAESAPILQ EQQQQQNSQY EGNEEDYGDS LIHLNIQENH YFITRDQLMS 70 80 90 100 110 120 LPESLLLCLF PSGVFLDRCG QVITNLTRDD EVYIVNFPPD CFEYIMEIYT KAHDDLYNHP 130 140 150 160 170 180 VEKFFDRPSS SFVSNAKGFF GLSSNNSISS NNEQDILHQK PAIIVLREDL DYYCVPQEEF 190 200 210 220 230 240 QFDSTNEENN EDLLRHFMAQ VKMAAGSYLT SKTSIFQGLY SSNRLKQQQQ QQKIEKGSNS 250 260 270 280 290 300 SSNTKSTSKK LGPAEQHLMD MLCSSGFTKE TCWGNRTQET GKTVISSLSL CRLANETTEG 310 320 330 340 350 360 FRQKFNEAKA KWEAEHKPSQ DNFITPMQSN ISINSLSASK SNSTISTARN LTSGSTAPAT 370 380 390 400 410 420 ARDKRKSRLS KLADNVRSHS SSRHSSQTRS KPPELPKLYD LVPKPNINAK LLLFWRKPAR 430 440 450 460 470 480 KCWWGEEDIE LEVEVFGSWK DESKKIIELI LPTNVDPEAE LHKIIVPVRL HIRRVWTLEL SVIGVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcript characterisation, gene disruption and nucleotide sequence of the Saccharomyces cerevisiae WH12 gene." Kelly D.E., Trevethick J., Mountain H., Sudbery P.E. Gene 66:205-213(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression." Kaida D., Yashiroda H., Toh-e A., Kikuchi Y. Genes Cells 7:543-552(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PSR1 AND MSN2.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380 AND SER-386, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130 AND SER-131, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M21089 Genomic DNA. Translation: AAA35218.1. Z74951 Genomic DNA. Translation: CAA99234.1. BK006948 Genomic DNA. Translation: DAA10825.1.
PIR	COBYW2. S66917.
RefSeq	NP_014686.1. NM_001183462.1.
3D structure databases
ProteinModelPortal	P12611.
SMR	P12611. Positions 39-111.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2573N.
IntAct	P12611. 11 interactions.
MINT	MINT-427278.
STRING	4932.YOR043W.
Proteomic databases
PaxDb	P12611.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR043W; YOR043W; YOR043W.
GeneID	854208.
KEGG	sce:YOR043W.
Organism-specific databases
CYGD	YOR043w.
SGD	S000005569. WHI2.
Phylogenomic databases
eggNOG	NOG83078.
HOGENOM	HOG000187763.
OrthoDB	EOG4C8C2W.
Gene expression databases
Genevestigator	P12611.
GermOnline	YOR043W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.710.10. 2 hits.
InterPro	IPR011333. BTB/POZ_fold. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	976056.

Entry information

Entry name

WHI2_YEAST

Accession

Primary (citable) accession number: P12611
Secondary accession number(s): D6W2A9, Q08413

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program