P12609 (VANA_PSES9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vanillate O-demethylase oxygenase subunit
EC=1.14.13.82

Alternative name(s):
4-hydroxy-3-methoxybenzoate demethylase

Gene names

Name:

vanA

Organism

Pseudomonas sp. (strain ATCC 19151)

Taxonomic identifier

315 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Vanillate + O2 + NADH = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde.
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Xenobiotic degradation; vanillyl-alcohol degradation.
Subunit structure	This demethylase system consists of two proteins: an oxygenase and an oxygenase reductase.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Lignin degradation
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro vanillate monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Vanillate O-demethylase oxygenase subunit		PRO_0000085058
Regions
	Domain	1 – 84	84	Rieske
Sites
	Metal binding	24	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	26	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	43	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	46	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12609 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: BAEA8D469E92BF43	FASTA	329	36,579
	10 20 30 40 50 60 MICNERMVIY RGAGQRVAAL EDFCPHRGAP LSLGSIQDGK LVCGYHGLVM DCDGRTASMP 70 80 90 100 110 120 AQRVQAFPCI RAFPAQERHG FIWVWPGDAA LADPALIPHL EWAENPAWAY GGGLYHIACD 130 140 150 160 170 180 YRLMIDNLMD LTHETYVHAS SIGQKEIDEA PVSTRVEGDR LITGRFMEGI LAPPFWRAAL 190 200 210 220 230 240 RGNGLADDVP VDRWQICRFT PPSHVLIEVG VAHAGRGGYD APADCKASSI VVDFITPETD 250 260 270 280 290 300 TSIWYFWGMA RSFRPEDNEL TARIREGQGT IFAEDLEMLE QQQRNLLAWP ERPLLKLNID 310 320 AGGVQSRRII ERLVSAERAA EAQLIGRQA

References

[1]	"Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase." Brunel F., Davison J. J. Bacteriol. 170:4924-4930(1988) [PubMed: 3170489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22077 Genomic DNA. Translation: AAA26019.1.
3D structure databases
ProteinModelPortal	P12609.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
Pfam	PF00355. Rieske. 1 hit. [Graphical view]
SUPFAM	SSF50022. Rieske_dom. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VANA_PSES9

Accession

Primary (citable) accession number: P12609

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 28, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program