Reviewed, UniProtKB/Swiss-Prot P12577 (L_PI3H4)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Human parainfluenza 3 virus (strain Wash/47885/57) (HPIV-3) (Human parainfluenza 3 virus (strain NIH 47885))

Taxonomic identifier

11217 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	VirionPotential. CytoplasmBy similarity.
Sequence similarities	Belongs to the paramyxoviruses L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2233	2233	Large structural protein		PRO_0000142733
Regions
	Domain	656 – 840	185	RdRp catalytic
	Nucleotide binding	1805 – 1814	10	ATP Potential
Experimental info
	Sequence conflict	26	1	K → R in AAA46857. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12577-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: DBBB8B5DE74B4638	FASTA	2,233	255,806
	10 20 30 40 50 60 MDTESNNGTV SDILYPECHL NSPIVKGKIA QLHTIMSLPQ PYDMDDDSIL VITRQKIKLN 70 80 90 100 110 120 KLDKRQRSIR RLKLILTEKV NDLGKYTFIR YPEMSKEMFK LHIPGINSKV TELLLKADRT 130 140 150 160 170 180 YSQMTDGLRD LWINVLSKLA SKNDGSNYDL NEEINNISKV HTTYKSDKWY NPFKTWFTIK 190 200 210 220 230 240 YDMRRLQKAR NEVTFNMGKD YNLLEDQKNF LLIHPELVLI LDKQNYNGYL ITPELVLPYC 250 260 270 280 290 300 DVVEGRWNIS ACAKLDPKLQ SMYQKGNNLW EVIDKLFPIM GEKTFDVISL LEPLALSLIQ 310 320 330 340 350 360 THDPVKQLRG AFLNHVLSEM ELIFESRESI KEFLSVDYID KILDIFNKST IDEIAEIFSF 370 380 390 400 410 420 FRTFGHPPLE ASIAAEKVRK YMYIGKQLKF DTINKCHAIF CTIIINGYRE RHGGQWPPVT 430 440 450 460 470 480 LPDHAHEFII NAYGSNSAIS YENAVDYYQS FIGIKFNKFI EPQLDEDLTI YMKDKALSPK 490 500 510 520 530 540 KSNWDTVSPA SNLLYRTNAS NESRRLVEKF IADSKFDPNQ ILDYVESGDW LDDPEFNISY 550 560 570 580 590 600 SLKEKEIKQE GRLFAKMTYK MRATQVLSET LLANNIGKFF QENGMVKGEI ELLKRLTTIS 610 620 630 640 650 660 ISGVPRYNEV YNNSKSHTDD LKTYNKISNL NLSSNQKSKK FEFKSTDIYN DGYETVSCFL 670 680 690 700 710 720 TTDLKKYCLN WRYESTALFG ETCNQIFGLN KLFNWLHPRL EGSTIYVGDP YCPPSDKEHI 730 740 750 760 770 780 SLEDHPDSGF YVHNPRGGIE GFCQKLWTLI SISAIHLAAV RIGVRVTAMV QGDNQAIAVT 790 800 810 820 830 840 TRVPNNYDYR VKKEIVYKDV VRFFDSLREV MDDLGHELKL NETIISSKMF IYSKRIYYDG 850 860 870 880 890 900 RILPQALKAL SRCVFWSETV IDETRSASSN LATSFAKAIE NGYSPVLGYA CSIFKNIQQL 910 920 930 940 950 960 YIALGMNINP TITQNIKDLY FRNPNWMQYA SLIPASVGGF NYMAMSRCFV RNIGDPSVAA 970 980 990 1000 1010 1020 LADIKRFIKA NLLDRSVLYR IMNQEPGESS FLDWASDPYS CNLPQSQNIT TMIKNITARN 1030 1040 1050 1060 1070 1080 VLQDSPNPLL SGLFTNTMIE EDEELAEFLM DRKVILPRVA HDILDNSLTG IRNAIAGMLD 1090 1100 1110 1120 1130 1140 TTKSLIRVGI NRGGLTYSLL RKISNYDLVQ YETLSRTLRL IVSDKIRYED MCSVDLAIAL 1150 1160 1170 1180 1190 1200 RQKMWIHLSG GRMISGLETP DPLELLSGVI ITGSEHCKIC YSSDGTNPYT WMYLPGNIKI 1210 1220 1230 1240 1250 1260 GSAETGISSL RVPYFGSVTD ERSEAQLGYI KNLSKPAKAA IRIAMIYTWA FGNDEISWME 1270 1280 1290 1300 1310 1320 ASQIAQTRAN FTLDSLKILT PVATSTNLSH RLKDTATQMK FSSTSLIRVS RFITMSNDNM 1330 1340 1350 1360 1370 1380 SIKEANETKD TNLIYQQIML TGLSVFEYLF RLEETTGHNP IVMHLHIEDE CCIKESFNDE 1390 1400 1410 1420 1430 1440 HINPESTLEL IRYPESNEFI YDKDPLKDVD LSKLMVIKDH SYTIDMNYWD DTDIIHAISI 1450 1460 1470 1480 1490 1500 CTAITIADTM SQLDRDNLKE IIVIANDDDI NSLITEFLTL DILVFLKTFG GLLVNQFAYT 1510 1520 1530 1540 1550 1560 LYSLKTEGRD LIWDYIMRTL RDTSHSILKV LSNALSHPKV FKRFWDCGVL NPIYGPNTAS 1570 1580 1590 1600 1610 1620 QDQIKLALSI CEYSLDLFMR EWLNGVSLEI YICDSDMEVA NDRKQAFISR HLSFVCCLAE 1630 1640 1650 1660 1670 1680 IASFGPNLLN LTYLERLDLL KQYLELNIKD DPTLKYVQIS GLLIKSFPST VTYVRKTAIK 1690 1700 1710 1720 1730 1740 YLRIRGISPP EVIDDWDPIE DENMLDNIVK TINDNCNKDN KGNKINNFWG LALKNYQVLK 1750 1760 1770 1780 1790 1800 IRSITSDSDN NDRSDASTGG LTLPQGGNYL SHQLRLFGIN STSCLKALEL SQILMKEVNK 1810 1820 1830 1840 1850 1860 DQDRLFLGEG AGAMLACYDA TLGPAVNYYN SGLNITDVIG QRELKIFPSE VSLVGKKLGN 1870 1880 1890 1900 1910 1920 VTQILNRVKV LFNGNPNSTW IGNMECETLI WSELNDKSIG LVHCDMEGAI GKSEETVLHE 1930 1940 1950 1960 1970 1980 HYSVIRITYL IGDDDVVLIS KIIPTITPNW SRILYLYKLY WKDVSIISLK TSNPASTELY 1990 2000 2010 2020 2030 2040 LISKDAYCTI MEPSEVVLSK LKRLSLLEEN NLLKWIILSK KKNNEWLHHE IKEGERDYGV 2050 2060 2070 2080 2090 2100 MRPYHMALQI FGFQINLNHL AKEFLSTPDL TNINNIIQSF QRTIKDVLFE WINITHDGKR 2110 2120 2130 2140 2150 2160 HKLGGRYNIF PLKNKGKLRL LSRRLVLSWI SLSLSTRLLT GRFPDEKFEH RAQTGYVSLP 2170 2180 2190 2200 2210 2220 DTDLESLKLL SKNTIKNYRE CIGSISYWFL TKEVKILMKL IGGAKLLGIP RQYKEPEEQL 2230 LEDYNQHDEF DID

References

[1]	"Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein." Galinski M.S., Mink M.A., Pons M.W. Virology 165:499-510(1988) [PubMed: 2841798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses." Storey D.G., Cote M.-J., Dimock K., Kang C.Y. Intervirology 27:69-80(1987) [PubMed: 2822598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-26.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M21649 Genomic RNA. Translation: AAA46854.1. M20402 mRNA. Translation: AAA46857.1.
PIR	B46451.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR016269. RNA-dir_RNA_pol_paramyxovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_PI3H4

Accession

Primary (citable) accession number: P12577

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)