Reviewed, UniProtKB/Swiss-Prot P12576 (L_MEASE)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Measles virus (strain Edmonston) (Subacute sclerose panencephalitis virus)

Taxonomic identifier

11235 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Morbillivirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	VirionPotential. CytoplasmBy similarity.
Sequence similarities	Belongs to the paramyxoviruses L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2183	2183	Large structural protein		PRO_0000142729
Regions
	Domain	656 – 840	185	RdRp catalytic
	Nucleotide binding	1785 – 1794	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P12576-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 1B0B03CA2E2B6EA5	FASTA	2,183	247,648
	10 20 30 40 50 60 MDSLSVNQIL YPEVHLDSPI VTNKIVAILE YARVPHAYSL EDPTLCQNIK HRLKNGFSNQ 70 80 90 100 110 120 MIINNVEVGN VIKSKLRSYP AHSHIPYPNC NQDLFNIEDK ESTRKIRELL KKGNSLYSKV 130 140 150 160 170 180 SDKVFQCLRD TNSRLGLGSE LREDIKEKVI NLGVYMHSSQ WFEPFLFWFT VKTEMRSVIK 190 200 210 220 230 240 SQTHTCHRRR HTPVFFTGSS VELLISRDLV AIISKESQHV YYLTFELVLM YCDVIEGRLM 250 260 270 280 290 300 TETAMTIDAR YTELLGRVRY MWKLIDGFFP ALGNPTYQIV AMLEPLSLAY LQLRDITVEL 310 320 330 340 350 360 RGAFLNHCFT EIHDVLDQNG FSDEGTYHEL IEALDYIFIT DDIHLTGEIF SFFRSFGHPR 370 380 390 400 410 420 LEAVTAAENV RKYMNQPKVI VYETLMKGHA IFCGIIINGY RDRHGGSWPP LTLPLHAADT 430 440 450 460 470 480 IRNAQASGEG LTHEQCVDNW KSFAGVKFGC FMPLSLDSDL TMYLKDKALA ALQREWDSVY 490 500 510 520 530 540 PKEFLRYDPP KGTGSRRLVD VFLNDSSFDP YDVIMYVVSG AYLHDPEFNL SYSLQEKEIK 550 560 570 580 590 600 ETGRLFAKMT YKMRACQVIA ENLISNGIGK YFKDNGMAKD EQDLTKALHT LAVSGVPKDL 610 620 630 640 650 660 KESHRGGPVL KTYSRSPVHT STRNVRAAKG FIGFPQVIRQ DQDTDHPENM EAYETVSAFI 670 680 690 700 710 720 TTDLKKYCLN WRYETISLFA QRLNEIYGLP SFFQWLHKRL ETSVLYVSDP HCPPDLDAHI 730 740 750 760 770 780 PLYKVPNDQI FIKYPMGGIE GYCQKLWTIS TIPYLYLAAY ESGVRIASLV QGDNQTIAVT 790 800 810 820 830 840 KRVPSTWPYN LKKREAARVT RDYFVILRQR LHDIGHHLKA NETIVSSHFF VYSKGIYYDG 850 860 870 880 890 900 LLVSQSLKSI ARCVFWSETI VDETRAACSN IATTMAKSIE RGYDRYLAYS LNFLKVIQQI 910 920 930 940 950 960 LISLGFTINS TMTRDVVIPL LTNNDLLIRM ALLPAPIGGM NYLNMSRLFV RNIGDPVTSS 970 980 990 1000 1010 1020 IADLKRMILA SLMPEETLHQ VMTQQPGDSS FLDWASDPYS ANLVCVQSIT RLLKNITARF 1030 1040 1050 1060 1070 1080 VLIHSPNPML KGLFHDDSKE EDEGLAAFLM DRHIIVPRAA HEILDHSVTG ARESIAGMLD 1090 1100 1110 1120 1130 1140 TTKGLIRASM RKGGLTSRVI TRLSNYDYEQ FRAGMVLLTG RKRNVLIDKE SCSVQLARAL 1150 1160 1170 1180 1190 1200 RSHMWARLAR GRPIYGLEVP DVLESMRGHL IRRHETCVIC ECGSVNYGWF FVPSGCQLDD 1210 1220 1230 1240 1250 1260 IDKETSSLRV PYIGSTTDER TDMKLAFVRA PSRSLRSAVR IATVYSWAYG DDDSSWNEAW 1270 1280 1290 1300 1310 1320 LLARQRANVS LEELRVITPI STSTNLAHRL RDRSTQVKYS GTSLVRVARY TTISNDNLSF 1330 1340 1350 1360 1370 1380 VISDKKVDTN FIYQQGMLLG LGVLETLFRL EKDTGSSNTV LHLHVETDCC VIPMIDHPRI 1390 1400 1410 1420 1430 1440 PSSRKLELRA ELCTNPLIYD NAPLIDRDAT RLYTQSHRRH LVEFVTWSTP QLYHILAKST 1450 1460 1470 1480 1490 1500 ALSMIDLVTK FEKDHMNEIS ALIGDDDINS FITEFLLIEP RLFTIYLGQC AAINWAFDVH 1510 1520 1530 1540 1550 1560 YHRPSGKYQM GELLSSFLSR MSKGVFKVLV NALSHPKIYK KFWHCGIIEP IHGPSLDAQN 1570 1580 1590 1600 1610 1620 LHTTVCNMVY TCYMTYLDLL LNEELEEFTF LLCESDEDVV PDRFDNIQAK HLCVLADLYC 1630 1640 1650 1660 1670 1680 QPGTCPPIQG LRPVEKCAVL TDHIKAEAML SPAGSSWNIN PIIVDHYSCS LTYLRRGSIK 1690 1700 1710 1720 1730 1740 QIRLRVDPGF IFDALAEVNV SQPKIGSNNI SNMSIKAFRP PHDDVAKLLK DINTSKHNLP 1750 1760 1770 1780 1790 1800 ISGGNLANYE IHAFRRIGLN SSACYKAVEI STLIRRCLEP GEDGLFLGEG SGSMLITYKE 1810 1820 1830 1840 1850 1860 ILKLSKCFYN SGVSANSRSG QRELAPYPSE VGLVEHRMGV GNIVKVLFNG RPEVTWVGSV 1870 1880 1890 1900 1910 1920 DCFNFIVSNI PTSSVGFIHS DIETLPDKDT IEKLEELAAI LSMALLLGKI GSILVIKLMP 1930 1940 1950 1960 1970 1980 FSGDFVQGFI SYVGSHYREV NLVYPRYSNF ISTESYLVMT DLKANRLMNP EKIKQQIIES 1990 2000 2010 2020 2030 2040 SVRTSPGLIG HILSIKQLSC IQAIVGDAVS RGDINPTLKK LTPIEQVLIN CGLAINGPKL 2050 2060 2070 2080 2090 2100 CKELIHHDVA SGQDGLLNSI LILYRELARF KDNQRSQQGM FHAYPVLVSS RQRELISRIT 2110 2120 2130 2140 2150 2160 RKFWGHILLY SGNRKLINKF IQNLKSGYLI LDLHQNIFVK NLSKSEKQII MTGGLKREWV 2170 2180 FKVTVKETKE WYKLVGYSAL IKD

References

[1]	"Measles virus L protein evidences elements of ancestral RNA polymerase." Blumberg B.M., Crowley J.C., Silverman J.I., Menonna J., Cook S.D., Dowling P.C. Virology 164:487-497(1988) [PubMed: 2835864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases." Cattaneo R., Schmid A., Spielhofer P., Kaelin K., Baczko K., Meulen V., Pardowitz J., Flanagan S., Rima B.K., Udem S.A. Virology 173:415-425(1989) [PubMed: 2596022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20865 mRNA. Translation: AAA46430.1. K01711 Genomic RNA. Translation: AAA75501.1.
PIR	ZLNZMV. A28919.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR016269. RNA-dir_RNA_pol_paramyxovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_MEASE

Accession

Primary (citable) accession number: P12576

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 25, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)