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P12559 (HN_NDVH4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18
Gene names
Name:HN
OrganismNewcastle disease virus (strain B1-Hitchner/47) (NDV)
Taxonomic identifier11181 [NCBI]
Taxonomic lineageViruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Avulavirus ›
Virus hostGallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.

Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Hemagglutinin-neuraminidase
PRO_0000142611

Regions

Topological domain1 – 2626Intravirion Potential
Transmembrane27 – 4721Helical; Potential
Topological domain48 – 577530Virion surface Potential

Amino acid modifications

Glycosylation1191N-linked (GlcNAc...); by host Potential
Glycosylation3411N-linked (GlcNAc...); by host Potential
Glycosylation4331N-linked (GlcNAc...); by host Potential
Glycosylation4811N-linked (GlcNAc...); by host Potential
Glycosylation5381N-linked (GlcNAc...); by host Potential

Natural variations

Natural variant2001L → S.
Natural variant2031H → Y.
Natural variant3241T → S.

Sequences

Sequence LengthMass (Da)Tools
P12559 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: A7BCF780AA8FF70E

FASTA57763,241
        10         20         30         40         50         60 
MDRAVSQVAL ENDEREAKNT WRLIFRIAIL FLTVVTLAIS VASLLYSMGA STPSDLVGIP 

        70         80         90        100        110        120 
TRISRAEEKI TSTLGSNQDV VDRIYKQVAL ESPLALLNTE TTIMNAITSL SYQINGAANN 

       130        140        150        160        170        180 
SGWGAPIHDP DYIGGIGKEL IVDDASDVTS FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM 

       190        200        210        220        230        240 
SATHYCYTHN VILSGCRDHL HSHQYLALGV LRTSATGRVF FSTLRSINLD DTQNRKSCSV 

       250        260        270        280        290        300 
SATPLGCDML CSKATETEEE DYNSAVPTRM VHGRLGFDGQ YHEKDLDVTT LFGDWVANYP 

       310        320        330        340        350        360 
GVGGGSFIDS RVWFSVYGGL KPNTPSDTVQ EGKYVIYKRY NDTCPDEQDY QIRMAKSSYK 

       370        380        390        400        410        420 
PGRFGGKRIQ QAILSIKVST SLGEDPVLTV PPNTVTLMGA EGRILTVGTS HFLYQRGSSY 

       430        440        450        460        470        480 
FSPALLYPMT VSNKTATLHS PYTFNAFTRP GSIPCQASAR CPNSCVTGVY TDPYPLIFYR 

       490        500        510        520        530        540 
NHTLRGVFGT MLDGEQARLN PASAVFDSTS RSRITRVSSS SIKAAYTTST CFKVVKTNKT 

       550        560        570 
YCLSIAEISN TLFGEFRIVP LLVEILKDDG VREARSG

« Hide

References

[1]"Cloning and nucleotide sequence of Newcastle disease virus hemagglutinin-neuraminidase mRNA: identification of a putative sialic acid binding site."
Jorgensen E.D., Collins P.L., Lomedico P.T.
Virology 156:12-24(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M16573 mRNA. Translation: AAA46668.1.
M24708 Genomic RNA. Translation: AAA46658.1.
PIRHNNZNC. A26355.

3D structure databases

ProteinModelPortalP12559.
SMRP12559. Positions 124-572.
ModBaseSearch...

Protein family/group databases

CAZyGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamPF00423. HN. 1 hit.
[Graphical view]
PIRSFPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMSSF50939. Sialidase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHN_NDVH4
AccessionPrimary (citable) accession number: P12559
Secondary accession number(s): P06158
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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