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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Newcastle disease virus (strain Chicken/Northern Ireland/Ulster/67) (NDV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Gene namesi
Name:HN
OrganismiNewcastle disease virus (strain Chicken/Northern Ireland/Ulster/67) (NDV)
Taxonomic identifieri11190 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeAvulavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626IntravirionSequence AnalysisAdd
BLAST
Transmembranei27 – 4721HelicalSequence AnalysisAdd
BLAST
Topological domaini48 – 616569Virion surfaceSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 616616Hemagglutinin-neuraminidasePRO_0000142618Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi341 – 3411N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi433 – 4331N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi481 – 4811N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi538 – 5381N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi600 – 6001N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FZHX-ray3.50A/B/C/D124-616[»]
ProteinModelPortaliP12558.
SMRiP12558. Positions 124-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR016285. Hemagglutn-neuramid.
IPR000665. Hemagglutn/HN.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P12558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRAVSQVAL ENDEREAKNT WRLVFRIAIL LLTVVTLAIS AAALAYSMEA
60 70 80 90 100
STPSDLIGIP TAISRAEEKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE
110 120 130 140 150
STIMNAITSL SYQINGAANS SGCGAPIHDP DYIGGIGKEL IVDDASDVTS
160 170 180 190 200
FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM SATHYCYTHN VILSGCRDHS
210 220 230 240 250
HSHQYLALGV LRTSATGRVF FSTLHSINLD DTQNRKSCSV SATPLGCDML
260 270 280 290 300
CSKVTETEEE DYNSAVPTSM VHGRLGFDGQ YHEKDLDVTT LFEDWVANYP
310 320 330 340 350
GVGGGSFIDN RVWFPVYGGL KPNSPSDTAQ EGKYVIYKRY NDTCPDEQDY
360 370 380 390 400
QIRMAKSSYK PGRFGGKRVQ QAILSIKVST SLGEDPVLTV PPNTVTLMGA
410 420 430 440 450
EGRVLTVGTS HFLYQRGSSY FSPALLYPMT VSNKTATLHS PYTFDAFTRP
460 470 480 490 500
GSVPCQASAR CPNSCVTGVY TDPYPLVFYR NHTLRGVFGT MLDDKQARLN
510 520 530 540 550
PVSAVFDSIS RSRITRVSSS STKAAYTTST CFKVVKTNKT YCLSIAEISN
560 570 580 590 600
TLFGEFRIVP LLVEILKDDG VREARAGRLS QLREGWKDDI VSPIFCDAKN
610
QTEYRRELES YAASWP
Length:616
Mass (Da):67,590
Last modified:October 1, 1989 - v1
Checksum:iE70D67796F6DDA36
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti74 – 741L → H.
Natural varianti74 – 741L → S.
Natural varianti259 – 2591E → K.
Natural varianti438 – 4381L → R.
Natural varianti495 – 4951K → E.
Natural varianti555 – 5562EF → GI.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00243 Genomic RNA. Translation: BAA00174.1.
M19478 Genomic RNA. Translation: AAA46677.1.
M24707 Genomic RNA. Translation: AAA46657.1.
PIRiB29823. HNNZU1.
C46328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00243 Genomic RNA. Translation: BAA00174.1.
M19478 Genomic RNA. Translation: AAA46677.1.
M24707 Genomic RNA. Translation: AAA46657.1.
PIRiB29823. HNNZU1.
C46328.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FZHX-ray3.50A/B/C/D124-616[»]
ProteinModelPortaliP12558.
SMRiP12558. Positions 124-572.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR016285. Hemagglutn-neuramid.
IPR000665. Hemagglutn/HN.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains."
    Millar N.S., Chambers P., Emmerson P.T.
    J. Gen. Virol. 69:613-620(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Structural features unique to each of the three antigenic sites on the hemagglutinin-neuraminidase protein of Newcastle disease virus."
    Gotoh B., Sakaguchi T., Nishikawa K., Inocencio N.M., Hamaguchi M., Toyoda T., Nagai Y.
    Virology 163:174-182(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
    Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
    Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHN_NDVU
AccessioniPrimary (citable) accession number: P12558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 24, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.