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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Newcastle disease virus (strain Italien/45) (NDV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Gene namesi
Name:HN
OrganismiNewcastle disease virus (strain Italien/45) (NDV)
Taxonomic identifieri11182 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeAvulavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626IntravirionSequence AnalysisAdd
BLAST
Transmembranei27 – 4721HelicalSequence AnalysisAdd
BLAST
Topological domaini48 – 571524Virion surfaceSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Hemagglutinin-neuraminidasePRO_0000142612Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi341 – 3411N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi433 – 4331N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi481 – 4811N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi508 – 5081N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi538 – 5381N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP12556.
SMRiP12556. Positions 124-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P12556-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRAVGRVAL ENEEREAKNT WRFVFRIAIF LLIVITLAIS AAALVYSMEA
60 70 80 90 100
STPGDLVGIP TVISRAEEKI TSALSSNQDV VDRIYKQVAL ESPLALLNTE
110 120 130 140 150
SVIMNAITSL SYQINGAANN SGCGAPVHDP DYIGGIGKEL IVDDASDVTS
160 170 180 190 200
FYPSAFQEHL NFIPAPTTGS GCTRIPSFDI SATHYCYTHN VILSGCRDHS
210 220 230 240 250
HSHQYLALGV LRTSATGRVF FSTLRSINLD DNQNRKSCSV SATPLGCDML
260 270 280 290 300
CSKITETEEE DYSSVTPTSM VHGRLGFDGQ YHEKDLDVIT LFKDWVANYP
310 320 330 340 350
GVGGGSFIDN RVWFPVYGGL KPNSPSDTAQ EGRYVIYKRY NDTCPDEQDY
360 370 380 390 400
QIRMAKSSYK PGRFGGKRVQ QAILSIKVST SLGEDPVLTV PPNTVTLMGP
410 420 430 440 450
EGRVLTVGTS HFLYQRGSSY FSPALLYPMT VNNKTATLHS PYTFNAFTRP
460 470 480 490 500
GSVPCQASAR CPNSCVTGVY TDPYPLVFHR NHTLRGVFGT MLDDKQARLN
510 520 530 540 550
PVSAVFDNIS RSRITRVSSS STKAAYTTST CFKVVKTNKT YCLSIAEISN
560 570
TLFGEFRIVP LLVEILKEDG V
Length:571
Mass (Da):62,605
Last modified:October 1, 1989 - v1
Checksum:iF1ECB12BEF46C00F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 42RA → LP.
Natural varianti52 – 521T → S.
Natural varianti75 – 751S → G.
Natural varianti216 – 2161T → A.
Natural varianti400 – 4012PE → AD.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18640 Genomic RNA. Translation: AAA46671.1.
M24715 Genomic RNA. Translation: AAA46665.1.
PIRiS07126. B36829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18640 Genomic RNA. Translation: AAA46671.1.
M24715 Genomic RNA. Translation: AAA46665.1.
PIRiS07126. B36829.

3D structure databases

ProteinModelPortaliP12556.
SMRiP12556. Positions 124-571.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The hemagglutinin-neuraminidase (HN) gene of Newcastle disease virus strain Italien (NDV Italien): comparison with HNs of other strains and expression by a vaccinia recombinant."
    Wemers C.D., de Henau S., Neyt C., Espion D., Letellier C., Meulemans G., Burny A.
    Arch. Virol. 97:101-113(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
    Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
    Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHN_NDVI
AccessioniPrimary (citable) accession number: P12556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.