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P12554

- HN_NDVA

UniProt

P12554 - HN_NDVA

Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Newcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.By similarity
    Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

    Protein family/group databases

    CAZyiGH83. Glycoside Hydrolase Family 83.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-neuraminidase (EC:3.2.1.18)
    Gene namesi
    Name:HN
    OrganismiNewcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
    Taxonomic identifieri11177 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeAvulavirus
    Virus hostiGallus gallus (Chicken) [TaxID: 9031]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 570570Hemagglutinin-neuraminidasePRO_0000142606Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi119 – 1191N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi432 – 4321N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi480 – 4801N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi507 – 5071N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi537 – 5371N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60382N.

    Structurei

    Secondary structure

    1
    570
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi130 – 1323
    Turni133 – 1353
    Beta strandi136 – 1383
    Beta strandi144 – 1463
    Helixi148 – 1503
    Beta strandi167 – 1704
    Beta strandi172 – 19221
    Turni193 – 1953
    Beta strandi202 – 21211
    Beta strandi218 – 22811
    Beta strandi238 – 2425
    Beta strandi245 – 2528
    Helixi257 – 2604
    Beta strandi269 – 2746
    Beta strandi280 – 2856
    Turni287 – 2937
    Beta strandi294 – 2996
    Beta strandi305 – 3073
    Beta strandi310 – 31910
    Helixi324 – 3274
    Turni328 – 3314
    Helixi347 – 35610
    Helixi361 – 3633
    Beta strandi368 – 3769
    Beta strandi378 – 38811
    Turni392 – 3943
    Beta strandi401 – 4066
    Beta strandi409 – 4146
    Beta strandi422 – 4309
    Beta strandi435 – 4373
    Beta strandi441 – 4477
    Beta strandi486 – 4916
    Beta strandi493 – 4986
    Beta strandi500 – 5056
    Beta strandi514 – 5163
    Beta strandi522 – 53312
    Turni534 – 5374
    Beta strandi538 – 54912
    Turni550 – 5523
    Beta strandi553 – 56513

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3T1EX-ray3.30A/B/E/F49-570[»]
    ProteinModelPortaliP12554.
    SMRiP12554. Positions 124-570.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12554.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2626IntravirionSequence AnalysisAdd
    BLAST
    Topological domaini49 – 570522Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei27 – 4822HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR000665. Hemagglutn-neuramid.
    IPR016285. Hemagglutn-neuramid_paramyxo.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF00423. HN. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P12554-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRAVCQVAL ENDEREAKNT WRLVFRIAIL LLTVMTLAIS AAALAYSMEA    50
    STPGDLVSIP TAISRAEGKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE 100
    SIIMNAITSL SYQINGAANN SGCGAPVHDP DYIGGIGKEL IVDDTSDVTS 150
    FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM SATHCYTHNV IFSGCRDHSH 200
    SHQYLALGVL RTSATGRVFF STLRSINLDD TQNRKSCSVS ATPLGCDMLC 250
    SKVTETEEED YNSVIPTSMV HGRLGFDGQY HEKDLDVTTL FGDWVANYPG 300
    VGGGSFIDNR VWFPVYGGLK PSSPSDTGQE GRYVIYKRYN DTCPDEQDYQ 350
    IRMAKSSYKP GRFGGKRVQQ AILSIKVSTS LGEDPVLTIP PNTVTLMGAE 400
    GRVLTVGTSH FLYQRGSSYF SPALLYPMTV NNNTATLHSP YTFNAFTRPG 450
    SVPCQASARC PNSCVTGVYT DPYPLVFHRN HTLRGVFGTM LDDEQARLNL 500
    VSAVFDNISR SRITRVSSSR TKAAYTTSTC FKVVKTNKTY CLSIAEISNT 550
    LFGEFRIVPL LVEILKDDGV 570
    Length:570
    Mass (Da):62,284
    Last modified:June 1, 1994 - v2
    Checksum:i96EF3FFED67179D7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841H → HY in AAA46662. (PubMed:2705297)Curated
    Sequence conflicti197 – 1971D → H in AAA46670. (PubMed:3037818)Curated
    Sequence conflicti259 – 2591E → Q in AAA46670. (PubMed:3037818)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22110 mRNA. Translation: AAA46670.1.
    M24712 Genomic RNA. Translation: AAA46662.1.
    PIRiB31110. HNNZAV.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22110 mRNA. Translation: AAA46670.1 .
    M24712 Genomic RNA. Translation: AAA46662.1 .
    PIRi B31110. HNNZAV.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3T1E X-ray 3.30 A/B/E/F 49-570 [» ]
    ProteinModelPortali P12554.
    SMRi P12554. Positions 124-570.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60382N.

    Protein family/group databases

    CAZyi GH83. Glycoside Hydrolase Family 83.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P12554.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR000665. Hemagglutn-neuramid.
    IPR016285. Hemagglutn-neuramid_paramyxo.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF00423. HN. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene encoding the Newcastle disease virus hemagglutinin-neuraminidase protein and comparisons of paramyxovirus hemagglutinin-neuraminidase protein sequences."
      McGinnes L.W., Wilde A., Morrison T.G.
      Virus Res. 7:187-202(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Characterization of the sites of proteolytic activation of Newcastle disease virus membrane glycoprotein precursors."
      Gorman J.J., Nestorowicz A., Mitchell S.J., Corino G.L., Selleck P.W.
      J. Biol. Chem. 263:12522-12531(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
      Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
      Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.

    Entry informationi

    Entry nameiHN_NDVA
    AccessioniPrimary (citable) accession number: P12554
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3