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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Newcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Gene namesi
Name:HN
OrganismiNewcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Taxonomic identifieri11177 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeAvulavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 26IntravirionSequence analysisAdd BLAST26
Transmembranei27 – 48HelicalSequence analysisAdd BLAST22
Topological domaini49 – 570Virion surfaceSequence analysisAdd BLAST522

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001426061 – 570Hemagglutinin-neuraminidaseAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi340N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi432N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi480N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi507N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi537N-linked (GlcNAc...); by hostSequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-60382N.

Structurei

Secondary structure

1570
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi81 – 85Combined sources5
Helixi86 – 89Combined sources4
Helixi92 – 111Combined sources20
Helixi130 – 132Combined sources3
Turni133 – 135Combined sources3
Beta strandi136 – 138Combined sources3
Beta strandi144 – 146Combined sources3
Helixi148 – 150Combined sources3
Beta strandi167 – 170Combined sources4
Beta strandi172 – 192Combined sources21
Turni193 – 195Combined sources3
Beta strandi202 – 212Combined sources11
Beta strandi218 – 228Combined sources11
Beta strandi238 – 242Combined sources5
Beta strandi245 – 252Combined sources8
Helixi257 – 260Combined sources4
Beta strandi269 – 274Combined sources6
Beta strandi280 – 285Combined sources6
Turni287 – 293Combined sources7
Beta strandi294 – 299Combined sources6
Beta strandi305 – 307Combined sources3
Beta strandi310 – 319Combined sources10
Helixi324 – 327Combined sources4
Turni328 – 331Combined sources4
Helixi347 – 356Combined sources10
Helixi361 – 363Combined sources3
Beta strandi368 – 376Combined sources9
Beta strandi378 – 388Combined sources11
Turni392 – 394Combined sources3
Beta strandi401 – 406Combined sources6
Beta strandi409 – 414Combined sources6
Beta strandi422 – 430Combined sources9
Beta strandi435 – 437Combined sources3
Beta strandi441 – 447Combined sources7
Beta strandi486 – 491Combined sources6
Beta strandi493 – 498Combined sources6
Beta strandi500 – 505Combined sources6
Beta strandi514 – 516Combined sources3
Beta strandi522 – 533Combined sources12
Turni534 – 537Combined sources4
Beta strandi538 – 549Combined sources12
Turni550 – 552Combined sources3
Beta strandi553 – 565Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T1EX-ray3.30A/B/E/F49-570[»]
ProteinModelPortaliP12554.
SMRiP12554.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12554.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR016285. Hemagglutn-neuramid.
IPR000665. Hemagglutn/HN.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P12554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRAVCQVAL ENDEREAKNT WRLVFRIAIL LLTVMTLAIS AAALAYSMEA
60 70 80 90 100
STPGDLVSIP TAISRAEGKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE
110 120 130 140 150
SIIMNAITSL SYQINGAANN SGCGAPVHDP DYIGGIGKEL IVDDTSDVTS
160 170 180 190 200
FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM SATHCYTHNV IFSGCRDHSH
210 220 230 240 250
SHQYLALGVL RTSATGRVFF STLRSINLDD TQNRKSCSVS ATPLGCDMLC
260 270 280 290 300
SKVTETEEED YNSVIPTSMV HGRLGFDGQY HEKDLDVTTL FGDWVANYPG
310 320 330 340 350
VGGGSFIDNR VWFPVYGGLK PSSPSDTGQE GRYVIYKRYN DTCPDEQDYQ
360 370 380 390 400
IRMAKSSYKP GRFGGKRVQQ AILSIKVSTS LGEDPVLTIP PNTVTLMGAE
410 420 430 440 450
GRVLTVGTSH FLYQRGSSYF SPALLYPMTV NNNTATLHSP YTFNAFTRPG
460 470 480 490 500
SVPCQASARC PNSCVTGVYT DPYPLVFHRN HTLRGVFGTM LDDEQARLNL
510 520 530 540 550
VSAVFDNISR SRITRVSSSR TKAAYTTSTC FKVVKTNKTY CLSIAEISNT
560 570
LFGEFRIVPL LVEILKDDGV
Length:570
Mass (Da):62,284
Last modified:June 1, 1994 - v2
Checksum:i96EF3FFED67179D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184H → HY in AAA46662 (PubMed:2705297).Curated1
Sequence conflicti197D → H in AAA46670 (PubMed:3037818).Curated1
Sequence conflicti259E → Q in AAA46670 (PubMed:3037818).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22110 mRNA. Translation: AAA46670.1.
M24712 Genomic RNA. Translation: AAA46662.1.
PIRiB31110. HNNZAV.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22110 mRNA. Translation: AAA46670.1.
M24712 Genomic RNA. Translation: AAA46662.1.
PIRiB31110. HNNZAV.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T1EX-ray3.30A/B/E/F49-570[»]
ProteinModelPortaliP12554.
SMRiP12554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60382N.

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12554.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR016285. Hemagglutn-neuramid.
IPR000665. Hemagglutn/HN.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHN_NDVA
AccessioniPrimary (citable) accession number: P12554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.