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P12554 (HN_NDVA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hemagglutinin-neuraminidase

EC=3.2.1.18
Gene names
Name:HN
OrganismNewcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Taxonomic identifier11177 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeAvulavirus
Virus hostGallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.

Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570Hemagglutinin-neuraminidase
PRO_0000142606

Regions

Topological domain1 – 2626Intravirion Potential
Transmembrane27 – 4822Helical; Potential
Topological domain49 – 570522Virion surface Potential

Amino acid modifications

Glycosylation1191N-linked (GlcNAc...); by host Potential
Glycosylation3401N-linked (GlcNAc...); by host Potential
Glycosylation4321N-linked (GlcNAc...); by host Potential
Glycosylation4801N-linked (GlcNAc...); by host Potential
Glycosylation5071N-linked (GlcNAc...); by host Potential
Glycosylation5371N-linked (GlcNAc...); by host Potential

Experimental info

Sequence conflict1841H → HY in AAA46662. Ref.3
Sequence conflict1971D → H in AAA46670. Ref.1
Sequence conflict2591E → Q in AAA46670. Ref.1

Secondary structure

........................................................................ 570
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P12554 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 96EF3FFED67179D7

FASTA57062,284
        10         20         30         40         50         60 
MNRAVCQVAL ENDEREAKNT WRLVFRIAIL LLTVMTLAIS AAALAYSMEA STPGDLVSIP 

        70         80         90        100        110        120 
TAISRAEGKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE SIIMNAITSL SYQINGAANN 

       130        140        150        160        170        180 
SGCGAPVHDP DYIGGIGKEL IVDDTSDVTS FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM 

       190        200        210        220        230        240 
SATHCYTHNV IFSGCRDHSH SHQYLALGVL RTSATGRVFF STLRSINLDD TQNRKSCSVS 

       250        260        270        280        290        300 
ATPLGCDMLC SKVTETEEED YNSVIPTSMV HGRLGFDGQY HEKDLDVTTL FGDWVANYPG 

       310        320        330        340        350        360 
VGGGSFIDNR VWFPVYGGLK PSSPSDTGQE GRYVIYKRYN DTCPDEQDYQ IRMAKSSYKP 

       370        380        390        400        410        420 
GRFGGKRVQQ AILSIKVSTS LGEDPVLTIP PNTVTLMGAE GRVLTVGTSH FLYQRGSSYF 

       430        440        450        460        470        480 
SPALLYPMTV NNNTATLHSP YTFNAFTRPG SVPCQASARC PNSCVTGVYT DPYPLVFHRN 

       490        500        510        520        530        540 
HTLRGVFGTM LDDEQARLNL VSAVFDNISR SRITRVSSSR TKAAYTTSTC FKVVKTNKTY 

       550        560        570 
CLSIAEISNT LFGEFRIVPL LVEILKDDGV 

« Hide

References

[1]"Nucleotide sequence of the gene encoding the Newcastle disease virus hemagglutinin-neuraminidase protein and comparisons of paramyxovirus hemagglutinin-neuraminidase protein sequences."
McGinnes L.W., Wilde A., Morrison T.G.
Virus Res. 7:187-202(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Characterization of the sites of proteolytic activation of Newcastle disease virus membrane glycoprotein precursors."
Gorman J.J., Nestorowicz A., Mitchell S.J., Corino G.L., Selleck P.W.
J. Biol. Chem. 263:12522-12531(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22110 mRNA. Translation: AAA46670.1.
M24712 Genomic RNA. Translation: AAA46662.1.
PIRHNNZAV. B31110.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3T1EX-ray3.30A/B/E/F49-570[»]
ProteinModelPortalP12554.
SMRP12554. Positions 124-570.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60382N.

Protein family/group databases

CAZyGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamPF00423. HN. 1 hit.
[Graphical view]
PIRSFPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP12554.

Entry information

Entry nameHN_NDVA
AccessionPrimary (citable) accession number: P12554
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries