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P12554

- HN_NDVA

UniProt

P12554 - HN_NDVA

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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Newcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Gene namesi
Name:HN
OrganismiNewcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Taxonomic identifieri11177 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeAvulavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626IntravirionSequence AnalysisAdd
BLAST
Transmembranei27 – 4822HelicalSequence AnalysisAdd
BLAST
Topological domaini49 – 570522Virion surfaceSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Hemagglutinin-neuraminidasePRO_0000142606Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi340 – 3401N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi432 – 4321N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi480 – 4801N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi507 – 5071N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi537 – 5371N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-60382N.

Structurei

Secondary structure

1
570
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi130 – 1323Combined sources
Turni133 – 1353Combined sources
Beta strandi136 – 1383Combined sources
Beta strandi144 – 1463Combined sources
Helixi148 – 1503Combined sources
Beta strandi167 – 1704Combined sources
Beta strandi172 – 19221Combined sources
Turni193 – 1953Combined sources
Beta strandi202 – 21211Combined sources
Beta strandi218 – 22811Combined sources
Beta strandi238 – 2425Combined sources
Beta strandi245 – 2528Combined sources
Helixi257 – 2604Combined sources
Beta strandi269 – 2746Combined sources
Beta strandi280 – 2856Combined sources
Turni287 – 2937Combined sources
Beta strandi294 – 2996Combined sources
Beta strandi305 – 3073Combined sources
Beta strandi310 – 31910Combined sources
Helixi324 – 3274Combined sources
Turni328 – 3314Combined sources
Helixi347 – 35610Combined sources
Helixi361 – 3633Combined sources
Beta strandi368 – 3769Combined sources
Beta strandi378 – 38811Combined sources
Turni392 – 3943Combined sources
Beta strandi401 – 4066Combined sources
Beta strandi409 – 4146Combined sources
Beta strandi422 – 4309Combined sources
Beta strandi435 – 4373Combined sources
Beta strandi441 – 4477Combined sources
Beta strandi486 – 4916Combined sources
Beta strandi493 – 4986Combined sources
Beta strandi500 – 5056Combined sources
Beta strandi514 – 5163Combined sources
Beta strandi522 – 53312Combined sources
Turni534 – 5374Combined sources
Beta strandi538 – 54912Combined sources
Turni550 – 5523Combined sources
Beta strandi553 – 56513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T1EX-ray3.30A/B/E/F49-570[»]
ProteinModelPortaliP12554.
SMRiP12554. Positions 124-570.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12554.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P12554-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRAVCQVAL ENDEREAKNT WRLVFRIAIL LLTVMTLAIS AAALAYSMEA
60 70 80 90 100
STPGDLVSIP TAISRAEGKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE
110 120 130 140 150
SIIMNAITSL SYQINGAANN SGCGAPVHDP DYIGGIGKEL IVDDTSDVTS
160 170 180 190 200
FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM SATHCYTHNV IFSGCRDHSH
210 220 230 240 250
SHQYLALGVL RTSATGRVFF STLRSINLDD TQNRKSCSVS ATPLGCDMLC
260 270 280 290 300
SKVTETEEED YNSVIPTSMV HGRLGFDGQY HEKDLDVTTL FGDWVANYPG
310 320 330 340 350
VGGGSFIDNR VWFPVYGGLK PSSPSDTGQE GRYVIYKRYN DTCPDEQDYQ
360 370 380 390 400
IRMAKSSYKP GRFGGKRVQQ AILSIKVSTS LGEDPVLTIP PNTVTLMGAE
410 420 430 440 450
GRVLTVGTSH FLYQRGSSYF SPALLYPMTV NNNTATLHSP YTFNAFTRPG
460 470 480 490 500
SVPCQASARC PNSCVTGVYT DPYPLVFHRN HTLRGVFGTM LDDEQARLNL
510 520 530 540 550
VSAVFDNISR SRITRVSSSR TKAAYTTSTC FKVVKTNKTY CLSIAEISNT
560 570
LFGEFRIVPL LVEILKDDGV
Length:570
Mass (Da):62,284
Last modified:June 1, 1994 - v2
Checksum:i96EF3FFED67179D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841H → HY in AAA46662. (PubMed:2705297)Curated
Sequence conflicti197 – 1971D → H in AAA46670. (PubMed:3037818)Curated
Sequence conflicti259 – 2591E → Q in AAA46670. (PubMed:3037818)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22110 mRNA. Translation: AAA46670.1.
M24712 Genomic RNA. Translation: AAA46662.1.
PIRiB31110. HNNZAV.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22110 mRNA. Translation: AAA46670.1 .
M24712 Genomic RNA. Translation: AAA46662.1 .
PIRi B31110. HNNZAV.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3T1E X-ray 3.30 A/B/E/F 49-570 [» ]
ProteinModelPortali P12554.
SMRi P12554. Positions 124-570.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60382N.

Protein family/group databases

CAZyi GH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P12554.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view ]
Pfami PF00423. HN. 1 hit.
[Graphical view ]
PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the gene encoding the Newcastle disease virus hemagglutinin-neuraminidase protein and comparisons of paramyxovirus hemagglutinin-neuraminidase protein sequences."
    McGinnes L.W., Wilde A., Morrison T.G.
    Virus Res. 7:187-202(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Characterization of the sites of proteolytic activation of Newcastle disease virus membrane glycoprotein precursors."
    Gorman J.J., Nestorowicz A., Mitchell S.J., Corino G.L., Selleck P.W.
    J. Biol. Chem. 263:12522-12531(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene."
    Sakaguchi T., Toyoda T., Gotoh B., Inocencio N.M., Kuma K., Miyata T., Nagai Y.
    Virology 169:260-272(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiHN_NDVA
AccessioniPrimary (citable) accession number: P12554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3