Reviewed, UniProtKB/Swiss-Prot P12547 (ORYZ_ASPOR)

Names and origin

Protein names

Recommended name:
    Oryzin
    EC=3.4.21.63
Alternative name(s):
    Alkaline proteinase
      Short name=ALP
    Aspergillus proteinase B
    Aspergillopeptidase B

Gene names

Name:	alk1
Synonyms:	alp
ORF Names:	AO090003001036

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Elastolytic enzyme probably acting as a virulence factor in invasive aspergillosis.
Catalytic activity	Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S8 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of catalytic activity Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Propeptide	22 – 121	100			PRO_0000027084
	Chain	122 – 403	282	Oryzin		PRO_0000027085
Sites
	Active site	162	1	Charge relay system By similarity
	Active site	193	1	Charge relay system By similarity
	Active site	349	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	253	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P12547-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: 39A8AC8B8790E010	FASTA	403	42,571
	10 20 30 40 50 60 MQSIKRTLLL LGAILPAVLG APVQETRRAA EKLPGKYIVT FKPGIDEAKI QEHTTWATNI 70 80 90 100 110 120 HQRSLERRGA TGGDLPVGIE RNYKINKFAA YAGSFDDATI EEIRKNEDVA YVEEDQIYYL 130 140 150 160 170 180 DGLTTQKSAP WGLGSISHKG QQSTDYIYDT SAGEGTYAYV VDSGVNVDHE EFEGRASKAY 190 200 210 220 230 240 NAAGGQHVDS IGHGTHVSGT IAGKTYGIAK KASILSVKVF QGESSSTSVI LDGFNWAAND 250 260 270 280 290 300 IVSKKRTSKA AINMSLGGGY SKAFNDAVEN AFEQGVLSVV AAGNENSDAG QTSPASAPDA 310 320 330 340 350 360 ITVAAIQKSN NRASFSNFGK VVDVFAPGQD ILSAWIGSSS ATNTISGTSM ATPHIVGLSL 370 380 390 400 YLAALENLDG PAAVTKRIKE LATKDVVKDV KGSPNLLAYN GNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A full length cDNA clone for the alkaline protease from Aspergillus oryzae: structural analysis and expression in Saccharomyces cerevisiae." Tatsumi H., Ogawa Y., Murakami S., Ishida Y., Murakami K., Masaki A., Kawabe H., Arimura H., Nakano E., Motai H. Mol. Gen. Genet. 219:33-38(1989) [PubMed: 2693947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 20386 / 460.
[2]	"Cloning and selective overexpression of an alkaline protease-encoding gene from Aspergillus oryzae." Cheevadhanarak S., Renno D.V., Saunders G., Holt G. Gene 108:151-155(1991) [PubMed: 1840548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: U 212.
[3]	"Isolation and characterization of the alkaline protease gene of Aspergillus oryzae." Murakami K., Ishida Y., Masaki A., Tatsumi H., Murakami S., Nakano E., Motai H., Kawabe H., Arimura H. Agric. Biol. Chem. 55:2807-2811(1991) [PubMed: 1368748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 20386 / 460.
[4]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.
[5]	"Cloning and sequencing of the alkaline protease cDNA from Aspergillus oryzae." Tatsumi H., Ohsawa M., Tsuji R.F., Murakami S., Nakano E., Motai H., Masaki A., Ishida Y., Murakami K., Kawabe H., Arimura H. Agric. Biol. Chem. 52:1887-1888(1988) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 122-403, PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17561 mRNA. Translation: CAA35594.1. S75278 Genomic DNA. Translation: AAB20819.1. X54726 Genomic DNA. Translation: CAA38527.2. Sequence problems. S79617 Genomic DNA. Translation: AAC60533.1. D10062 Genomic DNA. Translation: BAA00951.1. AP007155 Genomic DNA. Translation: BAE58142.1. D00350 mRNA. Translation: BAA00258.1.
PIR	SUASO. JU0278.
RefSeq	XP_001820144.1.
3D structure databases
HSSP	HSSP built from PDB template 1IC6 based on UniProtKB P06873.
ModBase	Search...
Protein family/group databases
MEROPS	S08.053.
Genome annotation databases
GeneID	5992127.
KEGG	aor:AO090003001036.
Enzyme and pathway databases
BRENDA	3.4.21.63. 2240.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR010259. Prot_inh_S8A. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF05922. Inhibitor_I9. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORYZ_ASPOR

Accession

Primary (citable) accession number: P12547
Secondary accession number(s): Q2UJX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)