P12546 (CERC_SCHMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cercarial protease EC=3.4.21.- Alternative name(s): Cercarial elastase
Organism	Schistosoma mansoni (Blood fluke)
Taxonomic identifier	6183 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protease cleaves elastin and thus facilitates penetration of schistosome parasite larvae through elastin-rich tissue of the host.
Enzyme regulation	Activated by an autocatalytic mechanism.
Tissue specificity	Acetabular (penetration) glands.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 27	8	Potential		PRO_0000028440
	Chain	28 – 264	237	Cercarial protease		PRO_0000028441
Regions
	Domain	28 – 264	237	Peptidase S1
Sites
	Active site	68	1	Charge relay system By similarity
	Active site	126	1	Charge relay system By similarity
	Active site	218	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	53 ↔ 69		By similarity
	Disulfide bond	192 ↔ 202		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12546 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: E2E5129A7C5D5010	FASTA	264	28,545
	10 20 30 40 50 60 MSNRWRFVVV VTLFTYCLTF ERVSTWLIRS GEPVQHPAEF PFIAFLTTER TMCTGSLVST 70 80 90 100 110 120 RAVLTAGHCV CSPLPVIRVS FLTLRNGDQQ GIHHQPSGVK VAPGYMPSCM SARQRRPIAQ 130 140 150 160 170 180 TLSGFDIAIV MLAQMVNLQS GIRVISLPQP SDIPPPGTGV FIVGYGRDDN DRDPSRKNGG 190 200 210 220 230 240 ILKKGRATIM ECRHATNGNP ICVKAGQNFG QLPAPGDSGG PLLPSLQGPV LGVVSHGVTL 250 260 PNLPDIIVEY ASVARMLDFV RSNI

References

[1]	"Cloning of the proteinase that facilitates infection by schistosome parasites." Newport G.R., McKerrow J.H., Hedstrom R., Petitt M., McGarrigle L., Barr P.J., Agabian N. J. Biol. Chem. 263:13179-13184(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03946 mRNA. Translation: AAA29864.1.
PIR	A28942.
3D structure databases
ProteinModelPortal	P12546.
ModBase	Search...
Protein family/group databases
MEROPS	S01.144.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CERC_SCHMA

Accession

Primary (citable) accession number: P12546

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)