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Protein

Plasminogen

Gene

PLG

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).By similarity

Catalytic activityi

Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.

Enzyme regulationi

Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Activated with catalytic amounts of streptokinase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134FibrinBy similarity1
Binding sitei136FibrinBy similarity1
Binding sitei136Omega-aminocarboxylic acidsBy similarity1
Binding sitei158Omega-aminocarboxylic acidsBy similarity1
Binding sitei172Omega-aminocarboxylic acidsBy similarity1
Binding sitei432Omega-aminocarboxylic acidsBy similarity1
Binding sitei445Omega-aminocarboxylic acidsBy similarity1
Active sitei622Charge relay systemBy similarity1
Active sitei665Charge relay systemBy similarity1
Active sitei760Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis, Tissue remodeling

Protein family/group databases

MEROPSiS01.233.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen (EC:3.4.21.7)
Cleaved into the following 4 chains:
Gene namesi
Name:PLG
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Unplaced

Subcellular locationi

  • Secreted By similarity

  • Note: Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000002805920 – 810PlasminogenAdd BLAST791
ChainiPRO_000002806020 – 580Plasmin heavy chain AAdd BLAST561
PeptideiPRO_000002806120 – 96Activation peptideAdd BLAST77
ChainiPRO_000002806297 – 580Plasmin heavy chain A, short formAdd BLAST484
ChainiPRO_0000028063581 – 810Plasmin light chain BAdd BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 73By similarity
Disulfide bondi53 ↔ 61By similarity
Disulfide bondi103 ↔ 181By similarity
Disulfide bondi124 ↔ 164By similarity
Disulfide bondi152 ↔ 176By similarity
Disulfide bondi185 ↔ 262By similarity
Disulfide bondi188 ↔ 316By similarity
Disulfide bondi206 ↔ 245By similarity
Disulfide bondi234 ↔ 257By similarity
Disulfide bondi275 ↔ 352By similarity
Disulfide bondi296 ↔ 335By similarity
Disulfide bondi324 ↔ 347By similarity
Glycosylationi365O-linked (GalNAc...)By similarity1
Disulfide bondi377 ↔ 454By similarity
Disulfide bondi398 ↔ 437By similarity
Disulfide bondi426 ↔ 449By similarity
Disulfide bondi481 ↔ 560By similarity
Disulfide bondi502 ↔ 543By similarity
Disulfide bondi531 ↔ 555By similarity
Disulfide bondi567 ↔ 685Interchain (between A and B chains)By similarity
Disulfide bondi577 ↔ 585Interchain (between A and B chains)By similarity
Modified residuei597PhosphoserineBy similarity1
Disulfide bondi607 ↔ 623By similarity
Modified residuei688PhosphoserineBy similarity1
Disulfide bondi699 ↔ 766By similarity
Disulfide bondi729 ↔ 745By similarity
Disulfide bondi756 ↔ 784By similarity

Post-translational modificationi

In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

Proteomic databases

PRIDEiP12545.

Interactioni

Subunit structurei

Interacts with CSPG4 and AMOT. Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation. Interacts (via Kringle 4 domain) with ADA; the interaction stimulates PLG activation when in complex with DPP4. Angiostatin: Interacts with ATP5A1; the interaction inhibits most of the angiogenic effects of angiostatin.By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000022281.

Structurei

3D structure databases

ProteinModelPortaliP12545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 98PANPROSITE-ProRule annotationAdd BLAST79
Domaini103 – 181Kringle 1PROSITE-ProRule annotationAdd BLAST79
Domaini184 – 262Kringle 2PROSITE-ProRule annotationAdd BLAST79
Domaini275 – 352Kringle 3PROSITE-ProRule annotationAdd BLAST78
Domaini377 – 454Kringle 4PROSITE-ProRule annotationAdd BLAST78
Domaini481 – 560Kringle 5PROSITE-ProRule annotationAdd BLAST80
Domaini581 – 808Peptidase S1PROSITE-ProRule annotationAdd BLAST228

Domaini

Kringle domains mediate interaction with CSPG4.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasminogen subfamily.PROSITE-ProRule annotation
Contains 5 kringle domains.PROSITE-ProRule annotation
Contains 1 PAN domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IDXR. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000112892.
HOVERGENiHBG004381.
InParanoidiP12545.
KOiK01315.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR003609. Pan_app.
IPR023317. Pept_S1A_plasmin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00051. Kringle. 5 hits.
PF00024. PAN_1. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001150. Plasmin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 5 hits.
SM00473. PAN_AP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 5 hits.
PROSITEiPS00021. KRINGLE_1. 5 hits.
PS50070. KRINGLE_2. 5 hits.
PS50948. PAN. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHKEVVLLL LLFLKSGQGE PLDDYVNTKG ASLFSITKKQ LGAGSIEECA
60 70 80 90 100
AKCEEEEEFT CRSFQYHSKE QQCVIMAENR KSSIVFRMRD VVLFEKKVYL
110 120 130 140 150
SECKTGNGKN YRGTMSKTRT GITCQKWSST SPHRPTFSPA THPSEGLEEN
160 170 180 190 200
YCRNPDNDGQ GPWCYTTDPE ERFDYCDIPE CEDECMHCSG ENYDGKISKT
210 220 230 240 250
MSGLECQAWD SQSPHAHGYI PSKFPNKNLK KNYCRNPDGE PRPWCFTTDP
260 270 280 290 300
NKRWELCDIP RCTTPPPSSG PTYQCLKGTG ENYRGDVAVT VSGHTCHGWS
310 320 330 340 350
AQTPHTHNRT PENFPCKNLD ENYCRNPDGE KAPWCYTTNS QVRWEYCKIP
360 370 380 390 400
SCESSPVSTE PLDPTAPPEL TPVVQECYHG DGQSYRGTSS TTTTGKKCQS
410 420 430 440 450
WSSMTPHWHE KTPENFPNAG LTMNYCRNPD ADKGPWCFTT DPSVRWEYCN
460 470 480 490 500
LKKCSGTEGS VAAPPPVAQL PDAETPSEED CMFGNGKGYR GKKATTVTGT
510 520 530 540 550
PCQEWAAQEP HSHRIFTPET NPRAGLEKNY CRNPDGDVGG PWCYTTNPRK
560 570 580 590 600
LFDYCDVPQC AASSFDCGKP QVEPKKCPGR VVGGCVAYPH SWPWQISLRT
610 620 630 640 650
RLGMHFCGGT LISPEWVLTA AHCLEKSSRP SFYKVILGAH REVHLEPHVQ
660 670 680 690 700
EIEVSKMFSE PARADIALLK LSSPAIITDK VIPACLPSPN YVVADRTECF
710 720 730 740 750
ITGWGETQGT YGAGLLKEAR LPVIENKVCN RYEFLNGTVK TTELCAGHLA
760 770 780 790 800
GGTDSCQGDS GGPLVCFEKD KYILQGVTSW GLGCARPNKP GVYVRVSRFV
810
TWIEGVMRNN
Length:810
Mass (Da):90,255
Last modified:October 1, 1989 - v1
Checksum:iA75E1C51A1A0F24A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04697 mRNA. Translation: AAA36901.1.
PIRiB32869. B30848.
RefSeqiNP_001036540.1. NM_001043075.1.
UniGeneiMmu.3024.

Genome annotation databases

GeneIDi703891.
KEGGimcc:703891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04697 mRNA. Translation: AAA36901.1.
PIRiB32869. B30848.
RefSeqiNP_001036540.1. NM_001043075.1.
UniGeneiMmu.3024.

3D structure databases

ProteinModelPortaliP12545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000022281.

Protein family/group databases

MEROPSiS01.233.

Proteomic databases

PRIDEiP12545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi703891.
KEGGimcc:703891.

Organism-specific databases

CTDi5340.

Phylogenomic databases

eggNOGiENOG410IDXR. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000112892.
HOVERGENiHBG004381.
InParanoidiP12545.
KOiK01315.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR003609. Pan_app.
IPR023317. Pept_S1A_plasmin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00051. Kringle. 5 hits.
PF00024. PAN_1. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001150. Plasmin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 5 hits.
SM00473. PAN_AP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 5 hits.
PROSITEiPS00021. KRINGLE_1. 5 hits.
PS50070. KRINGLE_2. 5 hits.
PS50948. PAN. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLMN_MACMU
AccessioniPrimary (citable) accession number: P12545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, resulting in 2 chains held together by 2 disulfide bonds. Without the inhibitor, the activation involves also removal of the activation peptide.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.