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Protein

Penton protein

Gene

L2

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Clathrin-mediated endocytosis of virus by host, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Penton protein
Short name:
CP-P
Alternative name(s):
Penton base protein
Protein III
Gene namesi
ORF Names:L2
OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifieri28285 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000004992 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Host nucleus 1 Publication

  • Note: Located at each vertex of the virion. Present in 60 copies per virion.

GO - Cellular componenti

  • host cell nucleus Source: UniProtKB-SubCell
  • T=25 icosahedral viral capsid Source: UniProtKB-KW
  • viral capsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, T=25 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002218731 – 571Penton proteinAdd BLAST571

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts (via the cell attachment site RGD) with host heterodimer ITGAV-ITGB5; this interaction promotes virus internalization. Interacts with host WWP1 and WWP2 (By similarity). Interacts with the fiber protein (via N-terminal tail region) (Probable). Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons.By similarityCurated2 Publications

Structurei

Secondary structure

1571
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi57 – 60Combined sources4
Turni79 – 82Combined sources4
Beta strandi83 – 86Combined sources4
Turni103 – 107Combined sources5
Beta strandi136 – 139Combined sources4
Beta strandi155 – 158Combined sources4
Beta strandi177 – 182Combined sources6
Turni183 – 186Combined sources4
Beta strandi193 – 199Combined sources7
Beta strandi215 – 218Combined sources4
Beta strandi221 – 223Combined sources3
Beta strandi228 – 230Combined sources3
Beta strandi251 – 253Combined sources3
Beta strandi257 – 260Combined sources4
Beta strandi275 – 277Combined sources3
Beta strandi289 – 294Combined sources6
Beta strandi394 – 397Combined sources4
Beta strandi405 – 407Combined sources3
Turni408 – 410Combined sources3
Beta strandi411 – 416Combined sources6
Helixi420 – 422Combined sources3
Turni430 – 432Combined sources3
Beta strandi457 – 459Combined sources3
Helixi482 – 484Combined sources3
Beta strandi485 – 489Combined sources5
Beta strandi494 – 496Combined sources3
Beta strandi503 – 505Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IYNelectron microscopy-M1-571[»]
3IZOelectron microscopy-A/B/C/D/E1-571[»]
4CWUX-ray3.50N1-571[»]
ProteinModelPortaliP12538.
SMRiP12538.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12538.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni336 – 348DisorderedSequence analysisAdd BLAST13

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi340 – 342Cell attachment siteSequence analysis3

Domaini

The cell attachment RGD motif is exposed at the virion surface and is involved in binding to the integrin heterodimer ITGAV-ITGB5.

Sequence similaritiesi

Belongs to the adenoviridae penton family.Curated

Family and domain databases

InterProiIPR002605. Adeno_Penton_B.
[Graphical view]
PfamiPF01686. Adeno_Penton_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRAAMYEEG PPPSYESVVS AAPVAAALGS PFDAPLDPPF VPPRYLRPTG
60 70 80 90 100
GRNSIRYSEL APLFDTTRVY LVDNKSTDVA SLNYQNDHSN FLTTVIQNND
110 120 130 140 150
YSPGEASTQT INLDDRSHWG GDLKTILHTN MPNVNEFMFT NKFKARVMVS
160 170 180 190 200
RLPTKDNQVE LKYEWVEFTL PEGNYSETMT IDLMNNAIVE HYLKVGRQNG
210 220 230 240 250
VLESDIGVKF DTRNFRLGFD PVTGLVMPGV YTNEAFHPDI ILLPGCGVDF
260 270 280 290 300
THSRLSNLLG IRKRQPFQEG FRITYDDLEG GNIPALLDVD AYQASLKDDT
310 320 330 340 350
EQGGGGAGGS NSSGSGAEEN SNAAAAAMQP VEDMNDHAIR GDTFATRAEE
360 370 380 390 400
KRAEAEAAAE AAAPAAQPEV EKPQKKPVIK PLTEDSKKRS YNLISNDSTF
410 420 430 440 450
TQYRSWYLAY NYGDPQTGIR SWTLLCTPDV TCGSEQVYWS LPDMMQDPVT
460 470 480 490 500
FRSTRQISNF PVVGAELLPV HSKSFYNDQA VYSQLIRQFT SLTHVFNRFP
510 520 530 540 550
ENQILARPPA PTITTVSENV PALTDHGTLP LRNSIGGVQR VTITDARRRT
560 570
CPYVYKALGI VSPRVLSSRT F
Length:571
Mass (Da):63,293
Last modified:October 1, 1989 - v1
Checksum:i89D77207F2786802
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73260 Genomic DNA. No translation available.
M22141 Genomic DNA. Translation: AAA42519.1.
PIRiJT0337. XZADH5.
RefSeqiAP_000206.1. AC_000008.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73260 Genomic DNA. No translation available.
M22141 Genomic DNA. Translation: AAA42519.1.
PIRiJT0337. XZADH5.
RefSeqiAP_000206.1. AC_000008.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IYNelectron microscopy-M1-571[»]
3IZOelectron microscopy-A/B/C/D/E1-571[»]
4CWUX-ray3.50N1-571[»]
ProteinModelPortaliP12538.
SMRiP12538.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12538.

Family and domain databases

InterProiIPR002605. Adeno_Penton_B.
[Graphical view]
PfamiPF01686. Adeno_Penton_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAPSP_ADE05
AccessioniPrimary (citable) accession number: P12538
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.