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P12538

- CAPSP_ADE05

UniProt

P12538 - CAPSP_ADE05

Protein

Penton protein

Gene

L2

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.2 Publications

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Clathrin-mediated endocytosis of virus by host, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penton protein
    Short name:
    CP-P
    Alternative name(s):
    Penton base protein
    Protein III
    Gene namesi
    ORF Names:L2
    OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
    Taxonomic identifieri28285 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000004992: Genome

    Subcellular locationi

    Virion 1 Publication. Host nucleus 1 Publication
    Note: Located at each vertex of the virion. Present in 60 copies per virion.

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. T=25 icosahedral viral capsid Source: UniProtKB-KW
    3. viral capsid Source: CACAO

    Keywords - Cellular componenti

    Capsid protein, Host nucleus, T=25 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 571571Penton proteinPRO_0000221873Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Interacts (via the cell attachment site RGD) with host heterodimer ITGAV-ITGB5; this interaction promotes virus internalization. Interacts with host WWP1 and WWP2 By similarity. Interacts with the fiber protein (via N-terminal tail region) Probable. Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons.By similarity2 PublicationsCurated

    Structurei

    Secondary structure

    1
    571
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi57 – 604
    Turni79 – 824
    Beta strandi83 – 864
    Turni103 – 1075
    Beta strandi136 – 1394
    Beta strandi155 – 1584
    Beta strandi177 – 1826
    Turni183 – 1864
    Beta strandi193 – 1997
    Beta strandi215 – 2184
    Beta strandi221 – 2233
    Beta strandi228 – 2303
    Beta strandi251 – 2533
    Beta strandi257 – 2604
    Beta strandi275 – 2773
    Beta strandi289 – 2946
    Beta strandi394 – 3974
    Beta strandi405 – 4073
    Turni408 – 4103
    Beta strandi411 – 4166
    Helixi420 – 4223
    Turni430 – 4323
    Beta strandi457 – 4593
    Helixi482 – 4843
    Beta strandi485 – 4895
    Beta strandi494 – 4963
    Beta strandi503 – 5053

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VSZX-ray3.50N1-571[»]
    3IYNelectron microscopy-M1-571[»]
    3IZOelectron microscopy-A/B/C/D/E1-571[»]
    ProteinModelPortaliP12538.
    SMRiP12538. Positions 49-569.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12538.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni336 – 34813DisorderedSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi340 – 3423Cell attachment siteSequence Analysis

    Domaini

    The cell attachment RGD motif is exposed at the virion surface and is involved in binding to the integrin heterodimer ITGAV-ITGB5.

    Sequence similaritiesi

    Belongs to the adenoviridae penton family.Curated

    Family and domain databases

    InterProiIPR002605. Adeno_Penton_B.
    [Graphical view]
    PfamiPF01686. Adeno_Penton_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRAAMYEEG PPPSYESVVS AAPVAAALGS PFDAPLDPPF VPPRYLRPTG    50
    GRNSIRYSEL APLFDTTRVY LVDNKSTDVA SLNYQNDHSN FLTTVIQNND 100
    YSPGEASTQT INLDDRSHWG GDLKTILHTN MPNVNEFMFT NKFKARVMVS 150
    RLPTKDNQVE LKYEWVEFTL PEGNYSETMT IDLMNNAIVE HYLKVGRQNG 200
    VLESDIGVKF DTRNFRLGFD PVTGLVMPGV YTNEAFHPDI ILLPGCGVDF 250
    THSRLSNLLG IRKRQPFQEG FRITYDDLEG GNIPALLDVD AYQASLKDDT 300
    EQGGGGAGGS NSSGSGAEEN SNAAAAAMQP VEDMNDHAIR GDTFATRAEE 350
    KRAEAEAAAE AAAPAAQPEV EKPQKKPVIK PLTEDSKKRS YNLISNDSTF 400
    TQYRSWYLAY NYGDPQTGIR SWTLLCTPDV TCGSEQVYWS LPDMMQDPVT 450
    FRSTRQISNF PVVGAELLPV HSKSFYNDQA VYSQLIRQFT SLTHVFNRFP 500
    ENQILARPPA PTITTVSENV PALTDHGTLP LRNSIGGVQR VTITDARRRT 550
    CPYVYKALGI VSPRVLSSRT F 571
    Length:571
    Mass (Da):63,293
    Last modified:October 1, 1989 - v1
    Checksum:i89D77207F2786802
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    M22141 Genomic DNA. Translation: AAA42519.1.
    PIRiJT0337. XZADH5.
    RefSeqiAP_000206.1. AC_000008.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    M22141 Genomic DNA. Translation: AAA42519.1 .
    PIRi JT0337. XZADH5.
    RefSeqi AP_000206.1. AC_000008.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VSZ X-ray 3.50 N 1-571 [» ]
    3IYN electron microscopy - M 1-571 [» ]
    3IZO electron microscopy - A/B/C/D/E 1-571 [» ]
    ProteinModelPortali P12538.
    SMRi P12538. Positions 49-569.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P12538.

    Family and domain databases

    InterProi IPR002605. Adeno_Penton_B.
    [Graphical view ]
    Pfami PF01686. Adeno_Penton_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Determination of the nucleotide sequence for the penton-base gene of human adenovirus type 5."
      Neumann R., Chroboczek J., Jacrot B.
      Gene 69:153-157(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
      Chroboczek J., Bieber F., Jacrot B.
      Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "De novo derivation of proteomes from transcriptomes for transcript and protein identification."
      Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.
      Nat. Methods 9:1207-1211(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 131-571.
    4. "Integrin alphavbeta5 is a primary receptor for adenovirus in CAR-negative cells."
      Lyle C., McCormick F.
      Virol. J. 7:148-148(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOST ITGAV-ITGB5 HETERODIMER.
    5. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (10.0 ANGSTROMS) OF 49-571.
    6. "Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks."
      Liu H., Jin L., Koh S.B., Atanasov I., Schein S., Wu L., Zhou Z.H.
      Science 329:1038-1043(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (3.6 ANGSTROMS) OF THE VIRAL PARTICLE, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CAPSID VERTEX PROTEIN.

    Entry informationi

    Entry nameiCAPSP_ADE05
    AccessioniPrimary (citable) accession number: P12538
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell By similarity.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3