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P12538

- CAPSP_ADE05

UniProt

P12538 - CAPSP_ADE05

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Protein

Penton protein

Gene

L2

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.2 Publications

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Clathrin-mediated endocytosis of virus by host, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Penton protein
Short name:
CP-P
Alternative name(s):
Penton base protein
Protein III
Gene namesi
ORF Names:L2
OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifieri28285 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000004992: Genome

Subcellular locationi

Virion 1 Publication. Host nucleus 1 Publication
Note: Located at each vertex of the virion. Present in 60 copies per virion.

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. T=25 icosahedral viral capsid Source: UniProtKB-KW
  3. viral capsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, T=25 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Penton proteinPRO_0000221873Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts (via the cell attachment site RGD) with host heterodimer ITGAV-ITGB5; this interaction promotes virus internalization. Interacts with host WWP1 and WWP2 (By similarity). Interacts with the fiber protein (via N-terminal tail region) (Probable). Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons.By similarity2 PublicationsCurated

Structurei

Secondary structure

1
571
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi57 – 604Combined sources
Turni79 – 824Combined sources
Beta strandi83 – 864Combined sources
Turni103 – 1075Combined sources
Beta strandi136 – 1394Combined sources
Beta strandi155 – 1584Combined sources
Beta strandi177 – 1826Combined sources
Turni183 – 1864Combined sources
Beta strandi193 – 1997Combined sources
Beta strandi215 – 2184Combined sources
Beta strandi221 – 2233Combined sources
Beta strandi228 – 2303Combined sources
Beta strandi251 – 2533Combined sources
Beta strandi257 – 2604Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi289 – 2946Combined sources
Beta strandi394 – 3974Combined sources
Beta strandi405 – 4073Combined sources
Turni408 – 4103Combined sources
Beta strandi411 – 4166Combined sources
Helixi420 – 4223Combined sources
Turni430 – 4323Combined sources
Beta strandi457 – 4593Combined sources
Helixi482 – 4843Combined sources
Beta strandi485 – 4895Combined sources
Beta strandi494 – 4963Combined sources
Beta strandi503 – 5053Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VSZX-ray3.50N1-571[»]
3IYNelectron microscopy-M1-571[»]
3IZOelectron microscopy-A/B/C/D/E1-571[»]
4CWUX-ray3.80N1-571[»]
ProteinModelPortaliP12538.
SMRiP12538. Positions 49-569.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12538.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni336 – 34813DisorderedSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi340 – 3423Cell attachment siteSequence Analysis

Domaini

The cell attachment RGD motif is exposed at the virion surface and is involved in binding to the integrin heterodimer ITGAV-ITGB5.

Sequence similaritiesi

Belongs to the adenoviridae penton family.Curated

Family and domain databases

InterProiIPR002605. Adeno_Penton_B.
[Graphical view]
PfamiPF01686. Adeno_Penton_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12538-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRAAMYEEG PPPSYESVVS AAPVAAALGS PFDAPLDPPF VPPRYLRPTG
60 70 80 90 100
GRNSIRYSEL APLFDTTRVY LVDNKSTDVA SLNYQNDHSN FLTTVIQNND
110 120 130 140 150
YSPGEASTQT INLDDRSHWG GDLKTILHTN MPNVNEFMFT NKFKARVMVS
160 170 180 190 200
RLPTKDNQVE LKYEWVEFTL PEGNYSETMT IDLMNNAIVE HYLKVGRQNG
210 220 230 240 250
VLESDIGVKF DTRNFRLGFD PVTGLVMPGV YTNEAFHPDI ILLPGCGVDF
260 270 280 290 300
THSRLSNLLG IRKRQPFQEG FRITYDDLEG GNIPALLDVD AYQASLKDDT
310 320 330 340 350
EQGGGGAGGS NSSGSGAEEN SNAAAAAMQP VEDMNDHAIR GDTFATRAEE
360 370 380 390 400
KRAEAEAAAE AAAPAAQPEV EKPQKKPVIK PLTEDSKKRS YNLISNDSTF
410 420 430 440 450
TQYRSWYLAY NYGDPQTGIR SWTLLCTPDV TCGSEQVYWS LPDMMQDPVT
460 470 480 490 500
FRSTRQISNF PVVGAELLPV HSKSFYNDQA VYSQLIRQFT SLTHVFNRFP
510 520 530 540 550
ENQILARPPA PTITTVSENV PALTDHGTLP LRNSIGGVQR VTITDARRRT
560 570
CPYVYKALGI VSPRVLSSRT F
Length:571
Mass (Da):63,293
Last modified:October 1, 1989 - v1
Checksum:i89D77207F2786802
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73260 Genomic DNA. No translation available.
M22141 Genomic DNA. Translation: AAA42519.1.
PIRiJT0337. XZADH5.
RefSeqiAP_000206.1. AC_000008.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73260 Genomic DNA. No translation available.
M22141 Genomic DNA. Translation: AAA42519.1 .
PIRi JT0337. XZADH5.
RefSeqi AP_000206.1. AC_000008.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VSZ X-ray 3.50 N 1-571 [» ]
3IYN electron microscopy - M 1-571 [» ]
3IZO electron microscopy - A/B/C/D/E 1-571 [» ]
4CWU X-ray 3.80 N 1-571 [» ]
ProteinModelPortali P12538.
SMRi P12538. Positions 49-569.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P12538.

Family and domain databases

InterProi IPR002605. Adeno_Penton_B.
[Graphical view ]
Pfami PF01686. Adeno_Penton_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Determination of the nucleotide sequence for the penton-base gene of human adenovirus type 5."
    Neumann R., Chroboczek J., Jacrot B.
    Gene 69:153-157(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
    Chroboczek J., Bieber F., Jacrot B.
    Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "De novo derivation of proteomes from transcriptomes for transcript and protein identification."
    Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.
    Nat. Methods 9:1207-1211(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 131-571.
  4. "Integrin alphavbeta5 is a primary receptor for adenovirus in CAR-negative cells."
    Lyle C., McCormick F.
    Virol. J. 7:148-148(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOST ITGAV-ITGB5 HETERODIMER.
  5. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (10.0 ANGSTROMS) OF 49-571.
  6. "Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks."
    Liu H., Jin L., Koh S.B., Atanasov I., Schein S., Wu L., Zhou Z.H.
    Science 329:1038-1043(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (3.6 ANGSTROMS) OF THE VIRAL PARTICLE, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CAPSID VERTEX PROTEIN.

Entry informationi

Entry nameiCAPSP_ADE05
AccessioniPrimary (citable) accession number: P12538
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3