P12522 (ATXB_LEIDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable proton ATPase 1B
EC=3.6.3.6

Alternative name(s):
LdH1B

Gene names

Name:

H1B

Organism

Leishmania donovani

Taxonomic identifier

5661 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	974 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + H2O + H+(In) = ADP + phosphate + H+(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Developmental stage	More abundant in amastigotes than promastigotes. Ref.1
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen-exporting ATPase activity, phosphorylative mechanism Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 974	974	Probable proton ATPase 1B		PRO_0000046181
Regions
	Transmembrane	93 – 112	20	Helical; Potential
	Transmembrane	118 – 137	20	Helical; Potential
	Transmembrane	265 – 286	22	Helical; Potential
	Transmembrane	295 – 321	27	Helical; Potential
	Transmembrane	631 – 651	21	Helical; Potential
	Transmembrane	662 – 684	23	Helical; Potential
	Transmembrane	698 – 712	15	Helical; Potential
	Transmembrane	738 – 761	24	Helical; Potential
	Transmembrane	813 – 840	28	Helical; Potential
	Transmembrane	869 – 887	19	Helical; Potential
Sites
	Active site	351	1	4-aspartylphosphate intermediate

Sequences

	Sequence		Length	Mass (Da)
	P12522 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 9F58AB61186556B1	FASTA	974	107,305
	10 20 30 40 50 60 MSSKKYELDA AAFEDKPESH SDAEMTPQKP QRRQSVLSKA VSEHDERATG PATDLLPPSK 70 80 90 100 110 120 GLTTEEAEEL LKKYGRNELP EKKTPSWLIY VRGLWGPMPA ALWIAIIIEF ALENWPDGAI 130 140 150 160 170 180 LFAIQIANAT IGWYETIKAG DAVAALKNSL KPTATVYRDS KWQQIDAAVL VPGDLVKLAS 190 200 210 220 230 240 GSAVPADCSI NEGVIDVDEA ALTGESLPVT MGPEHMPKMG SNVVRGEVEG TVQYTGSLTF 250 260 270 280 290 300 FGKTAALLQS VESDLGNIHV ILRRVMLALC AISFILCMCC FIYLLARFYE TFRHALQFAV 310 320 330 340 350 360 VVLVVSIPIA LEIVVTTTLA VGSKHLSKHK IIVTKLSAIE MMSGVNMLCS DKTGTLTLNK 370 380 390 400 410 420 MEIQEQCFTF EEGNDLKSTL VLAALAAKWR EPPRDALDTM VLGAADLDEC DNYQQLNFVP 430 440 450 460 470 480 FDPTTKRTAA TLVDRRSGEK FDVTKGAPHV ILQMVYNQDE INDEVVDIID SLAARGVRCL 490 500 510 520 530 540 SVAKTDQQGR WHMAGILTFL DPPRPDTKDT IRRSKEYGVD VKMITGDHLL IAKEMCRMLD 550 560 570 580 590 600 LDPNILTADK LPQIKDANDL PEDLGEKYGD MMLSVGGFAQ VFPEHKFMIV ETLRQRGYTC 610 620 630 640 650 660 AMTGDGVNDA PALKRADVGI AVHGATDAAR AAADMVLTEP GLSVVVEAML VSREVFQRML 670 680 690 700 710 720 SFLTYRISAT LQLVCFFFIA CFSLTPKAYG SVDPNFQFFH LPVLMFMLIT LLNDGCLMTI 730 740 750 760 770 780 GYDHVIPSER PQKWNLPVVF VSASILAAVA CGSSLMLLWI GLEGYSSQYY ENSWFHRLGL 790 800 810 820 830 840 AQLPQGKLVT MMYLKISISD FLTLFSSRTG GHFFFYVPPS PILFCGAIIS LLVSTMAASF 850 860 870 880 890 900 WHKSRPDNVL TEGLAWGQTN AEKLLPLWVW IYCIVWWFVQ DVVKVLAHIC MDAVDLFGCV 910 920 930 940 950 960 SDASGSGPIK PYSDDMKVNG FEPVKKPAEK STEKALNLSV SSGPHKALEG LREDTHVLNE 970 STSPVNAFSP KVKK

References

[1]	"A tandem pair of Leishmania donovani cation transporting ATPase genes encode isoforms that are differentially expressed." Meade J.C., Hudson K.M., Stringer S.L., Stringer J.R. Mol. Biochem. Parasitol. 33:81-91(1989) [PubMed: 2469011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Strain: MHOM/ET/67/L82.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF109296 Genomic DNA. Translation: AAA29228.1.
3D structure databases
ProteinModelPortal	P12522.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006534. ATPase_P-typ_PM_proton-efflux. IPR023298. ATPase_P-typ_TM_dom. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 2 hits. G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 2 hits.
PANTHER	PTHR24093:SF61. PTHR24093:SF61. 1 hit.
Pfam	PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01647. ATPase-IIIA_H. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATXB_LEIDO

Accession

Primary (citable) accession number: P12522

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	October 19, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)