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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A)
Taxonomic identifieri10969 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

  • Virion By similarity
  • Host endoplasmic reticulum lumen Curated

  • Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 50Sequence analysisAdd BLAST50
ChainiPRO_000014962051 – 326Outer capsid glycoprotein VP7Sequence analysisAdd BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid.By similarity
N-glycosylated.By similarity
Intramolecular disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 (By similarity).By similarity

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi81 – 86Combined sources6
Helixi87 – 93Combined sources7
Helixi98 – 108Combined sources11
Beta strandi116 – 122Combined sources7
Helixi124 – 129Combined sources6
Beta strandi135 – 143Combined sources9
Helixi146 – 148Combined sources3
Helixi152 – 159Combined sources8
Beta strandi160 – 167Combined sources8
Turni170 – 172Combined sources3
Beta strandi182 – 198Combined sources17
Beta strandi204 – 207Combined sources4
Helixi212 – 214Combined sources3
Beta strandi215 – 227Combined sources13
Beta strandi234 – 238Combined sources5
Turni241 – 243Combined sources3
Beta strandi244 – 254Combined sources11
Beta strandi258 – 264Combined sources7
Beta strandi273 – 276Combined sources4
Beta strandi284 – 287Combined sources4
Helixi292 – 301Combined sources10
Helixi303 – 308Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FMGX-ray3.40A51-326[»]
3GZTelectron microscopy3.80B/F/G/H/I/J/K/L/M/N/O/P/Q58-312[»]
SMRiP12476.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12476.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P12476-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYGIEYTTVL TFLISLILLN YILKSLTRMM DCIIYRFLFI VVILSPLLKA
60 70 80 90 100
QNYGINLPIT GSMDTAYANS TQEETFLTST LCLYYPTEAA TEINDNSWKD
110 120 130 140 150
TLSQLFLTKG WPTGSVYFKE YTDIASFSVD PQLYCDYNVV LMKYDATLQL
160 170 180 190 200
DMSELADLIL NEWLCNPMDI ALYYYQQTDE ANKWISMGSS CTIKVCPLNT
210 220 230 240 250
QTLGIGCLTT DTATFEEVAT AEKLVITDVV DGVNHKLDVT TATCTIRNCK
260 270 280 290 300
KLGPRENVAV IQVGGSDVLD ITADPTTAPQ TERMMRINWK KWWQVFYTVV
310 320
DYVNQIIQAM SKRSRSLNSA AFYNRI
Length:326
Mass (Da):36,989
Last modified:October 1, 1989 - v1
Checksum:i4913E7C879533A30
GO
Isoform 2 (identifier: P12476-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.
Show »
Length:297
Mass (Da):33,582
Checksum:i2B2C399A9A8A9051
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti32C → F in strain: Isolate MMU-18006. 1
Natural varianti171A → T in strain: Isolate MMU-18006. 1
Natural varianti324N → Y in strain: Isolate MMU-18006. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0386351 – 29Missing in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21650 Genomic RNA. Translation: AAA47346.1.
AF295303 mRNA. No translation available.
PIRiA29247. VGXRRH.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21650 Genomic RNA. Translation: AAA47346.1.
AF295303 mRNA. No translation available.
PIRiA29247. VGXRRH.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FMGX-ray3.40A51-326[»]
3GZTelectron microscopy3.80B/F/G/H/I/J/K/L/M/N/O/P/Q58-312[»]
SMRiP12476.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12476.

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiVP7_ROTRH
AccessioniPrimary (citable) accession number: P12476
Secondary accession number(s): P11855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.