P12472 (VP2_ROTRF) Reviewed, UniProtKB/Swiss-Prot
Last modified October 16, 2013. Version 63. History...
Names and origin
|Protein names||Recommended name:|
Inner capsid protein VP2
|Organism||Rotavirus A (strain Cow/France/RF/1975 G6-P6-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A) [Complete proteome]|
|Taxonomic identifier||10933 [NCBI]|
|Taxonomic lineage||Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A ›|
|Virus host||Bos taurus (Bovine) [TaxID: 9913]|
|Sequence length||880 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.
Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.
The N-terminus is involved in VP1 binding.
Belongs to the rotavirus VP2 family.
|Molecular function||Capsid protein|
Inner capsid protein
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Cellular_component||viral inner capsid|
Inferred from electronic annotation. Source: UniProtKB-KWviral nucleocapsid
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 880||880||Inner capsid protein VP2||PRO_0000149531|
|Region||1 – 92||92||Interaction with VP1 and VP3|
|Region||394 – 414||21||Hydrophobic|
|Region||422 – 442||21||Hydrophobic|
|Region||536 – 557||22||Leucine-zipper 1|
|Region||665 – 686||22||Leucine-zipper 2|
|||"Nucleotide sequence of the gene encoding for the RNA binding protein (VP2) of RF bovine rotavirus."|
Kumar A., Charpilienne A., Cohen J.
Nucleic Acids Res. 17:2126-2126(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"The N terminus of rotavirus VP2 is necessary for encapsidation of VP1 and VP3."|
Zeng C.Q.-Y., Estes M.K., Charpilienne A., Cohen J.
J. Virol. 72:201-208(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VP1 AND VP3.
|||"Identification of rotavirus VP6 residues located at the interface with VP2 that are essential for capsid assembly and transcriptase activity."|
Charpilienne A., Lepault J., Rey F.A., Cohen J.
J. Virol. 76:7822-7831(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VP6.
|||"Rotavirus nonstructural protein NSP5 interacts with major core protein VP2."|
Berois M., Sapin C., Erk I., Poncet D., Cohen J.
J. Virol. 77:1757-1763(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NSP5.
|X14057 Genomic RNA. Translation: CAA32213.1.|
X14057 Genomic RNA. Translation: CAA32215.1. Sequence problems.
3D structure databases
Protocols and materials databases
Family and domain databases
|InterPro||IPR007779. Rotavirus_VP2. |
|Pfam||PF05087. Rota_VP2. 1 hit. |
|ProDom||PD008866. Rota_VP2. 1 hit. |
[Graphical view] [Entries sharing at least one domain]
|Accession||Primary (citable) accession number: P12472|
Secondary accession number(s): Q86225
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families