P12429 (ANXA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A3

Alternative name(s):
35-alpha calcimedin
Annexin III
Annexin-3
Inositol 1,2-cyclic phosphate 2-phosphohydrolase
Lipocortin III
Placental anticoagulant protein III
Short name=PAP-III

Gene names

Name:	ANXA3
Synonyms:	ANX3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
Molecular function	Phospholipase A2 inhibitor
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from direct assay PubMed 9497492. Source: UniProtKB neutrophil degranulation Inferred from direct assay PubMed 9550422. Source: UniProtKB phagocytosis Inferred from direct assay PubMed 9497492. Source: UniProtKB positive regulation of angiogenesis Inferred from direct assay PubMed 16236264. Source: UniProtKB positive regulation of endothelial cell migration Inferred from direct assay PubMed 16236264. Source: UniProtKB positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 16236264. Source: UniProtKB
Cellular_component	phagocytic vesicle membrane Inferred from direct assay PubMed 7526843. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 7526843. Source: UniProtKB specific granule Inferred from direct assay PubMed 7526843. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Inferred from direct assay PubMed 2138016Ref.12. Source: UniProtKB phospholipase A2 inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.8 Ref.9

Chain

2 – 323

322

Annexin A3

PRO_0000067477

Regions

Repeat

27 – 87

61

Annexin 1

Repeat

99 – 159

61

Annexin 2

Repeat

183 – 243

61

Annexin 3

Repeat

258 – 318

61

Annexin 4

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.9

Natural variations

Natural variant

19

1

S → N. Ref.15
Corresponds to variant rs5951 [ dbSNP | Ensembl ].

VAR_013914

Natural variant

219

1

I → N. Ref.15
Corresponds to variant rs5948 [ dbSNP | Ensembl ].

VAR_013915

Natural variant

251

1

P → L. Ref.15
Corresponds to variant rs5949 [ dbSNP | Ensembl ].

VAR_013916

Natural variant

291

1

F → S. Ref.15
Corresponds to variant rs5941 [ dbSNP | Ensembl ].

VAR_013917

Experimental info

Sequence conflict

35

1

G → R in CAG28576. Ref.4

Sequence conflict

146

1

S → G AA sequence Ref.11

Sequence conflict

294

1

H → R AA sequence Ref.11

Secondary structure

1

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323

Helix Strand Turn

Details...

Helix

20 – 31

12

Beta strand

32 – 35

4

Helix

38 – 45

8

Helix

50 – 64

15

Helix

68 – 75

8

Helix

78 – 88

11

Helix

91 – 103

13

Beta strand

105 – 107

3

Helix

110 – 119

10

Helix

122 – 136

15

Helix

140 – 147

8

Helix

150 – 160

11

Helix

172 – 185

14

Turn

186 – 188

3

Beta strand

189 – 191

3

Helix

194 – 203

10

Helix

206 – 220

15

Helix

224 – 231

8

Helix

234 – 262

29

Beta strand

263 – 266

4

Helix

269 – 279

11

Turn

280 – 283

4

Helix

284 – 295

12

Helix

299 – 306

8

Helix

309 – 319

11

Sequences

	Sequence		Length	Mass (Da)
	P12429 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4128C715491FC132	FASTA	323	36,375
	10 20 30 40 50 60 MASIWVGHRG TVRDYPDFSP SVDAEAIQKA IRGIGTDEKM LISILTERSN AQRQLIVKEY 70 80 90 100 110 120 QAAYGKELKD DLKGDLSGHF EHLMVALVTP PAVFDAKQLK KSMKGAGTNE DALIEILTTR 130 140 150 160 170 180 TSRQMKDISQ AYYTVYKKSL GDDISSETSG DFRKALLTLA DGRRDESLKV DEHLAKQDAQ 190 200 210 220 230 240 ILYKAGENRW GTDEDKFTEI LCLRSFPQLK LTFDEYRNIS QKDIVDSIKG ELSGHFEDLL 250 260 270 280 290 300 LAIVNCVRNT PAFLAERLHR ALKGIGTDEF TLNRIMVSRS EIDLLDIRTE FKKHYGYSLY 310 320 SAIKSDTSGD YEITLLKICG GDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Five distinct calcium and phospholipid binding proteins share homology with lipocortin I." Pepinsky R.B., Tizard R., Mattaliano R.J., Sinclair L.K., Miller G.T., Browning J.L., Chow E.P., Burne C., Huang K.-S., Pratt D., Wachter L., Hession C., Frey A.Z., Wallner B.P. J. Biol. Chem. 263:10799-10811(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Chromosomal localization of the human annexin III (ANX3) gene." Tait J.F., Frankenberry D.A., Miao C.H., Killary A.M., Adler D.A., Disteche C.M. Genomics 10:441-448(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure and polymorphisms of the human annexin III (ANX3) gene." Tait J.F., Smith C., Xu L., Cookson B.T. Genomics 18:79-86(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[8]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8. Tissue: Platelet.
[9]	Bienvenut W.V., Heiserich L., Gottlieb E. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-9; 40-48; 105-120; 155-163; 249-257; 264-274 AND 280-288, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Colon carcinoma.
[10]	"Identity of inositol 1,2-cyclic phosphate 2-phosphohydrolase with lipocortin III." Ross T.S., Tait J.F., Majerus P.W. Science 248:605-607(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 41-102 AND 126-138.
[11]	"Placental anticoagulant proteins: isolation and comparative characterization four members of the lipocortin family." Tait J.F., Sakata M., McMullen B.A., Miao C.H., Funakoshi T., Hendrickson L.E., Fujikawa K. Biochemistry 27:6268-6276(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 41-79; 85-88; 104-119; 126-150 AND 217-323.
[12]	"Purification and characterization of an abundant cytosolic protein from human neutrophils that promotes Ca2(+)-dependent aggregation of isolated specific granules." Ernst J.D., Hoye E., Blackwood R.A., Jaye D. J. Clin. Invest. 85:1065-1071(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-55; 74-82; 105-126; 155-169; 177-209; 264-274 AND 305-315, CALCIUM-DEPENDENT BINDING TO PHOSPHOLIPIDS.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"The high-resolution crystal structure of human annexin III shows subtle differences with annexin V." Favier-Perron B., Lewit-Bentley A., Russo-Marie F. Biochemistry 35:1740-1744(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[15]	"Characterization of single-nucleotide polymorphisms in coding regions of human genes." Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS ASN-19; ASN-219; LEU-251 AND SER-291.
[16]	Erratum Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 23:373-373(1999)
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M20560 mRNA. Translation: AAA59496.1.
M63310 mRNA. Translation: AAA52284.1.
L20591 Genomic DNA. Translation: AAA16713.1.
CR407648 mRNA. Translation: CAG28576.1.
AK313945 mRNA. Translation: BAG36663.1.
CH471057 Genomic DNA. Translation: EAX05822.1.
BC000871 mRNA. Translation: AAH00871.1.

IPI

IPI00024095.

PIR

LUHU3. A47658.

RefSeq

NP_005130.1. NM_005139.2.

UniGene

Hs.480042.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AII	X-ray	1.95	A	1-323	[»]
1AXN	X-ray	1.78	A	2-323	[»]

ProteinModelPortal

P12429.

ModBase

Search...

Protein-protein interaction databases

IntAct

P12429. 6 interactions.

MINT

MINT-4998835.

STRING

9606.ENSP00000264908.

PTM databases

PhosphoSite

P12429.

Polymorphism databases

DMDM

113954.

2D gel databases

OGP

P12429.

SWISS-2DPAGE

P12429.

Proteomic databases

PaxDb

P12429.

PRIDE

P12429.

Protocols and materials databases

DNASU

306.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000264908; ENSP00000264908; ENSG00000138772.

GeneID

306.

KEGG

hsa:306.

UCSC

uc003hld.3. human.

Organism-specific databases

CTD

306.

GeneCards

GC04P079403.

HGNC

HGNC:541. ANXA3.

HPA

HPA013398.
HPA013431.

MIM

106490. gene.

neXtProt

NX_P12429.

PharmGKB

PA24831.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG267770.

HOGENOM

HOG000158803.

HOVERGEN

HBG061815.

InParanoid

P12429.

OMA

ALVEIAC.

OrthoDB

EOG434W6S.

PhylomeDB

P12429.

Gene expression databases

ArrayExpress

P12429.

Bgee

P12429.

CleanEx

HS_ANXA3.

Genevestigator

P12429.

GermOnline

ENSG00000138772. Homo sapiens.

Family and domain databases

Gene3D

1.10.220.10. 4 hits.

InterPro

IPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002390. AnnexinIII.
[Graphical view]

PANTHER

PTHR10502. PTHR10502. 1 hit.
PTHR10502:SF25. PTHR10502:SF25. 1 hit.

Pfam

PF00191. Annexin. 4 hits.
[Graphical view]

PRINTS

PR00196. ANNEXIN.
PR00199. ANNEXINIII.

SMART

SM00335. ANX. 4 hits.
[Graphical view]

SUPFAM

SSF47874. Annexin. 1 hit.

PROSITE

PS00223. ANNEXIN. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P12429.

GenomeRNAi

306.

NextBio

1235.

SOURCE

Search...

Entry information

Entry name

ANXA3_HUMAN

Accession

Primary (citable) accession number: P12429
Secondary accession number(s): B2R9W6, Q6LET2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 145 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.