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P12429 (ANXA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 154. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Annexin A3
Alternative name(s):
35-alpha calcimedin
Annexin III
Annexin-3
Inositol 1,2-cyclic phosphate 2-phosphohydrolase
Lipocortin III
Placental anticoagulant protein III
Short name=PAP-III
Gene names
Name:ANXA3
Synonyms:ANX3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.

Domain

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similarities

Belongs to the annexin family.

Contains 4 annexin repeats.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DomainAnnexin
Repeat
   LigandCalcium
Calcium/phospholipid-binding
   Molecular functionPhospholipase A2 inhibitor
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to bacterium

Inferred from direct assay PubMed 9497492. Source: UniProtKB

neutrophil degranulation

Inferred from direct assay PubMed 9550422. Source: UniProtKB

phagocytosis

Inferred from direct assay PubMed 9497492. Source: UniProtKB

positive regulation of angiogenesis

Inferred from direct assay PubMed 16236264. Source: UniProtKB

positive regulation of endothelial cell migration

Inferred from direct assay PubMed 16236264. Source: UniProtKB

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 16236264. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17205602. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 23376485. Source: UniProt

membrane

Inferred from direct assay PubMed 17205602. Source: UniProtKB

phagocytic vesicle membrane

Inferred from direct assay PubMed 7526843. Source: UniProtKB

plasma membrane

Inferred from direct assay PubMed 7526843. Source: UniProtKB

specific granule

Inferred from direct assay PubMed 7526843. Source: UniProtKB

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

calcium-dependent phospholipid binding

Inferred from direct assay PubMed 2138016Ref.12. Source: UniProtKB

calcium-dependent protein binding

Inferred from physical interaction PubMed 15226301. Source: AgBase

phospholipase A2 inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8 Ref.9
Chain2 – 323322Annexin A3
PRO_0000067477

Regions

Repeat27 – 8761Annexin 1
Repeat99 – 15961Annexin 2
Repeat183 – 24361Annexin 3
Repeat258 – 31861Annexin 4

Amino acid modifications

Modified residue21N-acetylalanine Ref.9

Natural variations

Natural variant191S → N. Ref.15
Corresponds to variant rs5951 [ dbSNP | Ensembl ].
VAR_013914
Natural variant2191I → N. Ref.15
Corresponds to variant rs5948 [ dbSNP | Ensembl ].
VAR_013915
Natural variant2511P → L. Ref.15
Corresponds to variant rs5949 [ dbSNP | Ensembl ].
VAR_013916
Natural variant2911F → S. Ref.15
Corresponds to variant rs5941 [ dbSNP | Ensembl ].
VAR_013917

Experimental info

Sequence conflict351G → R in CAG28576. Ref.4
Sequence conflict1461S → G AA sequence Ref.11
Sequence conflict2941H → R AA sequence Ref.11

Secondary structure

............................................. 323
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P12429 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 4128C715491FC132

FASTA32336,375
        10         20         30         40         50         60 
MASIWVGHRG TVRDYPDFSP SVDAEAIQKA IRGIGTDEKM LISILTERSN AQRQLIVKEY 

        70         80         90        100        110        120 
QAAYGKELKD DLKGDLSGHF EHLMVALVTP PAVFDAKQLK KSMKGAGTNE DALIEILTTR 

       130        140        150        160        170        180 
TSRQMKDISQ AYYTVYKKSL GDDISSETSG DFRKALLTLA DGRRDESLKV DEHLAKQDAQ 

       190        200        210        220        230        240 
ILYKAGENRW GTDEDKFTEI LCLRSFPQLK LTFDEYRNIS QKDIVDSIKG ELSGHFEDLL 

       250        260        270        280        290        300 
LAIVNCVRNT PAFLAERLHR ALKGIGTDEF TLNRIMVSRS EIDLLDIRTE FKKHYGYSLY 

       310        320 
SAIKSDTSGD YEITLLKICG GDD 

« Hide

References

« Hide 'large scale' references
[1]"Five distinct calcium and phospholipid binding proteins share homology with lipocortin I."
Pepinsky R.B., Tizard R., Mattaliano R.J., Sinclair L.K., Miller G.T., Browning J.L., Chow E.P., Burne C., Huang K.-S., Pratt D., Wachter L., Hession C., Frey A.Z., Wallner B.P.
J. Biol. Chem. 263:10799-10811(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Chromosomal localization of the human annexin III (ANX3) gene."
Tait J.F., Frankenberry D.A., Miao C.H., Killary A.M., Adler D.A., Disteche C.M.
Genomics 10:441-448(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure and polymorphisms of the human annexin III (ANX3) gene."
Tait J.F., Smith C., Xu L., Cookson B.T.
Genomics 18:79-86(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cervix.
[8]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-8.
Tissue: Platelet.
[9]Bienvenut W.V., Heiserich L., Gottlieb E.
Submitted (MAR-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-9; 40-48; 105-120; 155-163; 249-257; 264-274 AND 280-288, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Colon carcinoma.
[10]"Identity of inositol 1,2-cyclic phosphate 2-phosphohydrolase with lipocortin III."
Ross T.S., Tait J.F., Majerus P.W.
Science 248:605-607(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-102 AND 126-138.
[11]"Placental anticoagulant proteins: isolation and comparative characterization four members of the lipocortin family."
Tait J.F., Sakata M., McMullen B.A., Miao C.H., Funakoshi T., Hendrickson L.E., Fujikawa K.
Biochemistry 27:6268-6276(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 41-79; 85-88; 104-119; 126-150 AND 217-323.
[12]"Purification and characterization of an abundant cytosolic protein from human neutrophils that promotes Ca2(+)-dependent aggregation of isolated specific granules."
Ernst J.D., Hoye E., Blackwood R.A., Jaye D.
J. Clin. Invest. 85:1065-1071(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 42-55; 74-82; 105-126; 155-169; 177-209; 264-274 AND 305-315, CALCIUM-DEPENDENT BINDING TO PHOSPHOLIPIDS.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"The high-resolution crystal structure of human annexin III shows subtle differences with annexin V."
Favier-Perron B., Lewit-Bentley A., Russo-Marie F.
Biochemistry 35:1740-1744(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[15]"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS ASN-19; ASN-219; LEU-251 AND SER-291.
[16]Erratum
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 23:373-373(1999)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20560 mRNA. Translation: AAA59496.1.
M63310 mRNA. Translation: AAA52284.1.
L20591 Genomic DNA. Translation: AAA16713.1.
CR407648 mRNA. Translation: CAG28576.1.
AK313945 mRNA. Translation: BAG36663.1.
CH471057 Genomic DNA. Translation: EAX05822.1.
BC000871 mRNA. Translation: AAH00871.1.
CCDSCCDS3584.1.
PIRLUHU3. A47658.
RefSeqNP_005130.1. NM_005139.2.
XP_005263030.1. XM_005262973.1.
UniGeneHs.480042.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AIIX-ray1.95A1-323[»]
1AXNX-ray1.78A2-323[»]
ProteinModelPortalP12429.
SMRP12429. Positions 2-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106803. 12 interactions.
IntActP12429. 6 interactions.
MINTMINT-4998835.
STRING9606.ENSP00000264908.

PTM databases

PhosphoSiteP12429.

Polymorphism databases

DMDM113954.

2D gel databases

OGPP12429.
SWISS-2DPAGEP12429.

Proteomic databases

MaxQBP12429.
PaxDbP12429.
PRIDEP12429.

Protocols and materials databases

DNASU306.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264908; ENSP00000264908; ENSG00000138772.
GeneID306.
KEGGhsa:306.
UCSCuc003hld.3. human.

Organism-specific databases

CTD306.
GeneCardsGC04P079403.
HGNCHGNC:541. ANXA3.
HPAHPA013398.
HPA013431.
MIM106490. gene.
neXtProtNX_P12429.
PharmGKBPA24831.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG267770.
HOGENOMHOG000158803.
HOVERGENHBG061815.
InParanoidP12429.
KOK17089.
OMAHRGTVRD.
OrthoDBEOG74XS72.
PhylomeDBP12429.
TreeFamTF105452.

Gene expression databases

ArrayExpressP12429.
BgeeP12429.
CleanExHS_ANXA3.
GenevestigatorP12429.

Family and domain databases

Gene3D1.10.220.10. 4 hits.
InterProIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002390. AnnexinIII.
[Graphical view]
PANTHERPTHR10502:SF25. PTHR10502:SF25. 1 hit.
PfamPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSPR00196. ANNEXIN.
PR00199. ANNEXINIII.
SMARTSM00335. ANX. 4 hits.
[Graphical view]
PROSITEPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP12429.
GeneWikiAnnexin_A3.
GenomeRNAi306.
NextBio1235.
PROP12429.
SOURCESearch...

Entry information

Entry nameANXA3_HUMAN
AccessionPrimary (citable) accession number: P12429
Secondary accession number(s): B2R9W6, Q6LET2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM