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P12428

- BROWN_DROME

UniProt

P12428 - BROWN_DROME

Protein

Protein brown

Gene

bw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Part of a membrane-spanning permease system necessary for the transport of pigment precursors into pigment cells responsible for eye color. Brown and white dimerize for the transport of guanine.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi66 – 738ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. pigment binding Source: UniProtKB-KW

    GO - Biological processi

    1. eye pigment precursor transport Source: FlyBase
    2. ommochrome biosynthetic process Source: FlyBase
    3. sensory perception of sound Source: FlyBase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, Pigment

    Protein family/group databases

    TCDBi3.A.1.204.18. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein brown
    Gene namesi
    Name:bw
    ORF Names:CG17632
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0000241. bw.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 675675Protein brownPRO_0000093375Add
    BLAST

    Proteomic databases

    PRIDEiP12428.

    Expressioni

    Gene expression databases

    BgeeiP12428.

    Interactioni

    Subunit structurei

    Heterodimer of brown with white.

    Protein-protein interaction databases

    BioGridi63324. 1 interaction.
    DIPiDIP-387N.

    Structurei

    3D structure databases

    ProteinModelPortaliP12428.
    SMRiP12428. Positions 17-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei423 – 44119HelicalSequence AnalysisAdd
    BLAST
    Transmembranei454 – 47421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei504 – 52219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei531 – 55222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei565 – 58319HelicalSequence AnalysisAdd
    BLAST
    Transmembranei645 – 66521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 261228ABC transporterPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1131.
    GeneTreeiENSGT00740000114855.
    InParanoidiP12428.
    OMAiPTSDIFE.
    OrthoDBiEOG7TQV0H.
    PhylomeDBiP12428.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR013525. ABC_2_trans.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    IPR005284. Pigment_permease.
    [Graphical view]
    PfamiPF01061. ABC2_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00955. 3a01204. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P12428-4) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQESGGSSGQ GGPSLCLEWK QLNYYVPDQE QSNYSFWNEC RKKRELRILQ    50
    DASGHMKTGD LIAILGGSGA GKTTLLAAIS QRLRGNLTGD VVLNGMAMER 100
    HQMTRISSFL PQFEINVKTF TAYEHLYFMS HFKMHRRTTK AEKRQRVADL 150
    LLAVGLRDAA HTRIQQLSGG ERKRLSLAEE LITDPIFLFC DEPTTGLDSF 200
    SAYSVIKTLR HLCTRRRIAK HSLNQVYGED SFETPSGESS ASGSGSKSIE 250
    MEVVAESHES LLQTMRELPA LGVLSNSPNG THKKAAICSI HQPTSDIFEL 300
    FTHIILMDGG RIVYQGRTEQ AAKFFTDLGY ELPLNCNPAD FYLKTLADKE 350
    GKENAGAVLR AKYEHETDGL YSGSWLLARS YSGDYLKHVQ NFKKIRWIYQ 400
    VYLLMVRFMT EDLRNIRSGL IAFGFFMITA VTLSLMYSGI GGLTQRTVQD 450
    VGGSIFMLSN EMIFTFSYGV TYIFPAALPI IRREVGEGTY SLSAYYVALV 500
    LSFVPVAFFK GYVFLSVIYA SIYYTRGFLL YLSMGFLMSL SAVAAVGYGV 550
    FLSSLFESDK MASECAAPFD LIFLIFGGTY MNVDTVPGLK YLSLFFYSNE 600
    ALMYKFWIDI DNIDCPVNED HPCIKTGVEV LQQGSYRNAD YTYWLDCFSL 650
    VVVAVIFHIV SFGLVRRYIH RSGYY 675
    Length:675
    Mass (Da):75,943
    Last modified:October 1, 1989 - v1
    Checksum:i81DEBDF856F4F174
    GO
    Isoform B (identifier: P12428-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         317-328: RTEQAAKFFTDL → VVCAYAILRTIK
         329-675: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:328
    Mass (Da):36,487
    Checksum:iA89859424382476C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281D → A in AAC37214. (PubMed:7956072)Curated
    Sequence conflicti28 – 281D → A in AAA28398. (PubMed:7753025)Curated
    Sequence conflicti28 – 281D → A in AAM50157. (PubMed:12537569)Curated
    Sequence conflicti44 – 441R → P in AAA28398. (PubMed:7753025)Curated
    Sequence conflicti274 – 2741L → I in AAC37214. (PubMed:7956072)Curated
    Sequence conflicti274 – 2741L → I in AAA28398. (PubMed:7753025)Curated
    Sequence conflicti331 – 3311E → Q in AAC37214. (PubMed:7956072)Curated
    Sequence conflicti407 – 4071R → P in AAC37214. (PubMed:7956072)Curated
    Sequence conflicti638 – 6381N → T in AAC37214. (PubMed:7956072)Curated
    Sequence conflicti638 – 6381N → T in AAA28398. (PubMed:7753025)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei317 – 32812RTEQA…FFTDL → VVCAYAILRTIK in isoform B. 1 PublicationVSP_040308Add
    BLAST
    Alternative sequencei329 – 675347Missing in isoform B. 1 PublicationVSP_040309Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20630 mRNA. Translation: AAA28397.1.
    L23543 Genomic DNA. Translation: AAC37214.1.
    L05635 Genomic DNA. Translation: AAA28398.1.
    AE013599 Genomic DNA. Translation: AAF47020.3.
    AE013599 Genomic DNA. Translation: AAS64761.1.
    AY119503 mRNA. Translation: AAM50157.1.
    PIRiA31399. FYFFB.
    RefSeqiNP_523824.1. NM_079100.2. [P12428-4]
    UniGeneiDm.19990.

    Genome annotation databases

    EnsemblMetazoaiFBtr0072117; FBpp0072026; FBgn0000241. [P12428-4]
    GeneIDi37724.
    KEGGidme:Dmel_CG17632.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20630 mRNA. Translation: AAA28397.1 .
    L23543 Genomic DNA. Translation: AAC37214.1 .
    L05635 Genomic DNA. Translation: AAA28398.1 .
    AE013599 Genomic DNA. Translation: AAF47020.3 .
    AE013599 Genomic DNA. Translation: AAS64761.1 .
    AY119503 mRNA. Translation: AAM50157.1 .
    PIRi A31399. FYFFB.
    RefSeqi NP_523824.1. NM_079100.2. [P12428-4 ]
    UniGenei Dm.19990.

    3D structure databases

    ProteinModelPortali P12428.
    SMRi P12428. Positions 17-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 63324. 1 interaction.
    DIPi DIP-387N.

    Protein family/group databases

    TCDBi 3.A.1.204.18. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    PRIDEi P12428.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0072117 ; FBpp0072026 ; FBgn0000241 . [P12428-4 ]
    GeneIDi 37724.
    KEGGi dme:Dmel_CG17632.

    Organism-specific databases

    CTDi 37724.
    FlyBasei FBgn0000241. bw.

    Phylogenomic databases

    eggNOGi COG1131.
    GeneTreei ENSGT00740000114855.
    InParanoidi P12428.
    OMAi PTSDIFE.
    OrthoDBi EOG7TQV0H.
    PhylomeDBi P12428.

    Miscellaneous databases

    GenomeRNAii 37724.
    NextBioi 805087.
    PROi P12428.

    Gene expression databases

    Bgeei P12428.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR013525. ABC_2_trans.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    IPR005284. Pigment_permease.
    [Graphical view ]
    Pfami PF01061. ABC2_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00955. 3a01204. 1 hit.
    PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The brown protein of Drosophila melanogaster is similar to the white protein and to components of active transport complexes."
      Dreesen T.D., Johnson D.H., Henikoff S.
      Mol. Cell. Biol. 8:5206-5215(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    2. "Characterization of sequences responsible for trans-inactivation of the Drosophila brown gene."
      Martin-Morris L.E., Loughney K., Kershisnik E.O., Poortinga G., Henikoff S.
      Cold Spring Harb. Symp. Quant. Biol. 58:577-584(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster."
      Nitasaka E., Yamazaki T., Green M.M.
      Mol. Gen. Genet. 247:164-168(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: IG281.
    4. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    5. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Head.

    Entry informationi

    Entry nameiBROWN_DROME
    AccessioniPrimary (citable) accession number: P12428
    Secondary accession number(s): Q24264
    , Q7KVI1, Q7KVI2, Q8MRN9, Q9W1N7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3