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P12428 (BROWN_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length675 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Part of a membrane-spanning permease system necessary for the transport of pigment precursors into pigment cells responsible for eye color. Brown and white dimerize for the transport of guanine.

Subunit structure

Heterodimer of brown with white.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. [View classification]

Contains 1 ABC transporter domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P12428-4)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P12428-5)

The sequence of this isoform differs from the canonical sequence as follows:
     317-328: RTEQAAKFFTDL → VVCAYAILRTIK
     329-675: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 675675Protein brown
PRO_0000093375

Regions

Transmembrane423 – 44119Helical; Potential
Transmembrane454 – 47421Helical; Potential
Transmembrane504 – 52219Helical; Potential
Transmembrane531 – 55222Helical; Potential
Transmembrane565 – 58319Helical; Potential
Transmembrane645 – 66521Helical; Potential
Domain34 – 261228ABC transporter
Nucleotide binding66 – 738ATP By similarity

Natural variations

Alternative sequence317 – 32812RTEQA…FFTDL → VVCAYAILRTIK in isoform B.
VSP_040308
Alternative sequence329 – 675347Missing in isoform B.
VSP_040309

Experimental info

Sequence conflict281D → A in AAC37214. Ref.2
Sequence conflict281D → A in AAA28398. Ref.3
Sequence conflict281D → A in AAM50157. Ref.6
Sequence conflict441R → P in AAA28398. Ref.3
Sequence conflict2741L → I in AAC37214. Ref.2
Sequence conflict2741L → I in AAA28398. Ref.3
Sequence conflict3311E → Q in AAC37214. Ref.2
Sequence conflict4071R → P in AAC37214. Ref.2
Sequence conflict6381N → T in AAC37214. Ref.2
Sequence conflict6381N → T in AAA28398. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 81DEBDF856F4F174

FASTA67575,943
        10         20         30         40         50         60 
MQESGGSSGQ GGPSLCLEWK QLNYYVPDQE QSNYSFWNEC RKKRELRILQ DASGHMKTGD 

        70         80         90        100        110        120 
LIAILGGSGA GKTTLLAAIS QRLRGNLTGD VVLNGMAMER HQMTRISSFL PQFEINVKTF 

       130        140        150        160        170        180 
TAYEHLYFMS HFKMHRRTTK AEKRQRVADL LLAVGLRDAA HTRIQQLSGG ERKRLSLAEE 

       190        200        210        220        230        240 
LITDPIFLFC DEPTTGLDSF SAYSVIKTLR HLCTRRRIAK HSLNQVYGED SFETPSGESS 

       250        260        270        280        290        300 
ASGSGSKSIE MEVVAESHES LLQTMRELPA LGVLSNSPNG THKKAAICSI HQPTSDIFEL 

       310        320        330        340        350        360 
FTHIILMDGG RIVYQGRTEQ AAKFFTDLGY ELPLNCNPAD FYLKTLADKE GKENAGAVLR 

       370        380        390        400        410        420 
AKYEHETDGL YSGSWLLARS YSGDYLKHVQ NFKKIRWIYQ VYLLMVRFMT EDLRNIRSGL 

       430        440        450        460        470        480 
IAFGFFMITA VTLSLMYSGI GGLTQRTVQD VGGSIFMLSN EMIFTFSYGV TYIFPAALPI 

       490        500        510        520        530        540 
IRREVGEGTY SLSAYYVALV LSFVPVAFFK GYVFLSVIYA SIYYTRGFLL YLSMGFLMSL 

       550        560        570        580        590        600 
SAVAAVGYGV FLSSLFESDK MASECAAPFD LIFLIFGGTY MNVDTVPGLK YLSLFFYSNE 

       610        620        630        640        650        660 
ALMYKFWIDI DNIDCPVNED HPCIKTGVEV LQQGSYRNAD YTYWLDCFSL VVVAVIFHIV 

       670 
SFGLVRRYIH RSGYY 

« Hide

Isoform B [UniParc].

Checksum: A89859424382476C
Show »

FASTA32836,487

References

« Hide 'large scale' references
[1]"The brown protein of Drosophila melanogaster is similar to the white protein and to components of active transport complexes."
Dreesen T.D., Johnson D.H., Henikoff S.
Mol. Cell. Biol. 8:5206-5215(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]"Characterization of sequences responsible for trans-inactivation of the Drosophila brown gene."
Martin-Morris L.E., Loughney K., Kershisnik E.O., Poortinga G., Henikoff S.
Cold Spring Harb. Symp. Quant. Biol. 58:577-584(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster."
Nitasaka E., Yamazaki T., Green M.M.
Mol. Gen. Genet. 247:164-168(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IG281.
[4]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[5]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Head.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20630 mRNA. Translation: AAA28397.1.
L23543 Genomic DNA. Translation: AAC37214.1.
L05635 Genomic DNA. Translation: AAA28398.1.
AE013599 Genomic DNA. Translation: AAF47020.3.
AE013599 Genomic DNA. Translation: AAS64761.1.
AY119503 mRNA. Translation: AAM50157.1.
PIRFYFFB. A31399.
RefSeqNP_523824.1. NM_079100.2. [P12428-4]
UniGeneDm.19990.

3D structure databases

ProteinModelPortalP12428.
SMRP12428. Positions 17-266.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid63324. 1 interaction.
DIPDIP-387N.

Protein family/group databases

TCDB3.A.1.204.18. the atp-binding cassette (abc) superfamily.

Proteomic databases

PRIDEP12428.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0072117; FBpp0072026; FBgn0000241. [P12428-4]
GeneID37724.
KEGGdme:Dmel_CG17632.

Organism-specific databases

CTD37724.
FlyBaseFBgn0000241. bw.

Phylogenomic databases

eggNOGCOG1131.
GeneTreeENSGT00740000114855.
InParanoidP12428.
OMAPTSDIFE.
OrthoDBEOG7TQV0H.
PhylomeDBP12428.

Gene expression databases

BgeeP12428.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005284. Pigment_permease.
[Graphical view]
PfamPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00955. 3a01204. 1 hit.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi37724.
NextBio805087.
PROP12428.

Entry information

Entry nameBROWN_DROME
AccessionPrimary (citable) accession number: P12428
Secondary accession number(s): Q24264 expand/collapse secondary AC list , Q7KVI1, Q7KVI2, Q8MRN9, Q9W1N7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase