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P12427 (DMA_BPT2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA adenine methylase

EC=2.1.1.72
Alternative name(s):
Deoxyadenosyl-methyltransferase
M.EcoT2Dam
Gene names
Name:DAM
OrganismEnterobacteria phage T2 (Bacteriophage T2)
Taxonomic identifier10664 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
   Biological processDNA replication
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

site-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259DNA adenine methylase
PRO_0000087989

Sites

Binding site71S-adenosyl-L-methionine By similarity
Binding site111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site501S-adenosyl-L-methionine By similarity
Binding site1711S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
P12427 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: B3551303CFE71FD5

FASTA25930,439
        10         20         30         40         50         60 
MLGAIAYTGN KQSLLPELKP HFPKYDRFVD LFCGGLSVSL NVNGPVLAND IQEPIIEMYK 

        70         80         90        100        110        120 
RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP LLLYVLHFHG FSNMIRINDK 

       130        140        150        160        170        180 
GNFTTPFGKR TINKNSEKRF NHFKQNCDKI IFSSLHFKDV KILDGDFVYV DPPYLITVAD 

       190        200        210        220        230        240 
YNKFWSEEEE KDLLNLLDSL NDRGIKFGLS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV 

       250 
FNIYHSKEKN GTDEVYIFN 

« Hide

References

[1]"Molecular cloning, sequencing, and mapping of the bacteriophage T2 dam gene."
Miner Z., Hattman S.
J. Bacteriol. 170:5177-5184(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Single amino acid changes that alter the DNA sequence specificity of the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."
Miner Z., Schlagman S.L., Hattman S.
Nucleic Acids Res. 17:8149-8157(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22342 Genomic DNA. Translation: AAA32477.1.
PIRXYBPT2. A30195.

3D structure databases

ProteinModelPortalP12427.
SMRP12427. Positions 1-259.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2685. M.EcoT2Dam.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDMA_BPT2
AccessionPrimary (citable) accession number: P12427
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries