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P12427

- DMA_BPT2

UniProt

P12427 - DMA_BPT2

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Protein
DNA adenine methylase
Gene
DAM
Organism
Enterobacteria phage T2 (Bacteriophage T2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionine By similarity
Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei50 – 501S-adenosyl-L-methionine By similarity
Binding sitei171 – 1711S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi2685. M.EcoT2Dam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
Deoxyadenosyl-methyltransferase
M.EcoT2Dam
Gene namesi
Name:DAM
OrganismiEnterobacteria phage T2 (Bacteriophage T2)
Taxonomic identifieri10664 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259DNA adenine methylase
PRO_0000087989Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP12427.
SMRiP12427. Positions 1-259.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12427-1 [UniParc]FASTAAdd to Basket

« Hide

MLGAIAYTGN KQSLLPELKP HFPKYDRFVD LFCGGLSVSL NVNGPVLAND    50
IQEPIIEMYK RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP 100
LLLYVLHFHG FSNMIRINDK GNFTTPFGKR TINKNSEKRF NHFKQNCDKI 150
IFSSLHFKDV KILDGDFVYV DPPYLITVAD YNKFWSEEEE KDLLNLLDSL 200
NDRGIKFGLS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV FNIYHSKEKN 250
GTDEVYIFN 259
Length:259
Mass (Da):30,439
Last modified:October 1, 1989 - v1
Checksum:iB3551303CFE71FD5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22342 Genomic DNA. Translation: AAA32477.1.
PIRiA30195. XYBPT2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M22342 Genomic DNA. Translation: AAA32477.1 .
PIRi A30195. XYBPT2.

3D structure databases

ProteinModelPortali P12427.
SMRi P12427. Positions 1-259.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2685. M.EcoT2Dam.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, sequencing, and mapping of the bacteriophage T2 dam gene."
    Miner Z., Hattman S.
    J. Bacteriol. 170:5177-5184(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Single amino acid changes that alter the DNA sequence specificity of the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."
    Miner Z., Schlagman S.L., Hattman S.
    Nucleic Acids Res. 17:8149-8157(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiDMA_BPT2
AccessioniPrimary (citable) accession number: P12427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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