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Protein

DNA adenine methylase

Gene

DAM

Organism
Enterobacteria phage T2 (Bacteriophage T2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methyltransferase that methylates adenine residues in the dsDNA sequence GATC. May prevent degradation of viral DNA by the host restriction-modification antiviral defense system.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionineBy similarity
Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei50 – 501S-adenosyl-L-methionineBy similarity
Binding sitei171 – 1711S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication, Host-virus interaction, Restriction-modification system evasion by virus

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi2685. M.EcoT2Dam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA-(N(6)-adenine)-methyltransferaseBy similarity
Deoxyadenosyl-methyltransferase
M.EcoT2Dam
Gene namesi
Name:DAM
OrganismiEnterobacteria phage T2 (Bacteriophage T2)
Taxonomic identifieri10664 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259DNA adenine methylasePRO_0000087989Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP12427.
SMRiP12427. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGAIAYTGN KQSLLPELKP HFPKYDRFVD LFCGGLSVSL NVNGPVLAND
60 70 80 90 100
IQEPIIEMYK RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP
110 120 130 140 150
LLLYVLHFHG FSNMIRINDK GNFTTPFGKR TINKNSEKRF NHFKQNCDKI
160 170 180 190 200
IFSSLHFKDV KILDGDFVYV DPPYLITVAD YNKFWSEEEE KDLLNLLDSL
210 220 230 240 250
NDRGIKFGLS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV FNIYHSKEKN

GTDEVYIFN
Length:259
Mass (Da):30,439
Last modified:October 1, 1989 - v1
Checksum:iB3551303CFE71FD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22342 Genomic DNA. Translation: AAA32477.1.
PIRiA30195. XYBPT2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22342 Genomic DNA. Translation: AAA32477.1.
PIRiA30195. XYBPT2.

3D structure databases

ProteinModelPortaliP12427.
SMRiP12427. Positions 1-259.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2685. M.EcoT2Dam.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, sequencing, and mapping of the bacteriophage T2 dam gene."
    Miner Z., Hattman S.
    J. Bacteriol. 170:5177-5184(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Single amino acid changes that alter the DNA sequence specificity of the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."
    Miner Z., Schlagman S.L., Hattman S.
    Nucleic Acids Res. 17:8149-8157(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiDMA_BPT2
AccessioniPrimary (citable) accession number: P12427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 22, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.