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Protein

Adenine phosphoribosyltransferase

Gene

Aprt

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase (Aprt)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

ReactomeiR-DME-6798695. Neutrophil degranulation.
R-DME-74217. Purine salvage.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase (EC:2.4.2.7)
Short name:
APRT
Gene namesi
Name:Aprt
ORF Names:CG18315
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0000109. Aprt.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495121 – 182Adenine phosphoribosyltransferaseAdd BLAST182

Proteomic databases

PaxDbiP12426.
PRIDEiP12426.

Expressioni

Gene expression databases

BgeeiFBgn0000109.
GenevisibleiP12426. DM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi71230. 7 interactors.
DIPiDIP-22863N.
IntActiP12426. 1 interactor.
MINTiMINT-979410.
STRINGi7227.FBpp0088438.

Structurei

3D structure databases

ProteinModelPortaliP12426.
SMRiP12426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1712. Eukaryota.
COG0503. LUCA.
InParanoidiP12426.
KOiK00759.
OMAiKKHIGEY.
OrthoDBiEOG091G0XZC.
PhylomeDBiP12426.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr. 1 hit.
InterProiView protein in InterPro
IPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiView protein in PROSITE
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequencei

Sequence statusi: Complete.

P12426-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPSISAEDK LDYVKSKIGE YPNFPKEGIL FRDIFGALTD PKACVYLRDL
60 70 80 90 100
LVDHIRESAP EAEVIVGLDS RGFLFNLLIA TELGLGCAPI RKKGKLAGEV
110 120 130 140 150
VSVEYKLEYG IDTFELQKSA IKPGQKVVVV DDLLATGGSL VAATELIGKV
160 170 180
GGVVVESLVV MELVGLEGRK RLDGKVHSLI KY
Length:182
Mass (Da):19,690
Last modified:May 9, 2003 - v3
Checksum:iE69C129905AE4745
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64V → I in AAA28377 (PubMed:3125085).Curated1
Sequence conflicti64V → I in AAA57204 (PubMed:8492806).Curated1
Sequence conflicti111I → S in AAA28377 (PubMed:3125085).Curated1
Sequence conflicti111I → S in AAA57204 (PubMed:8492806).Curated1
Sequence conflicti148G → R in AAA28377 (PubMed:3125085).Curated1
Sequence conflicti148G → R in AAA57204 (PubMed:8492806).Curated1
Sequence conflicti160V → VV in AAA28377 (PubMed:3125085).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18432 mRNA. Translation: AAA28377.1.
L06280 Genomic DNA. Translation: AAA57204.1.
AE014296 Genomic DNA. Translation: AAF47589.1.
AY119066 mRNA. Translation: AAM50926.1.
PIRiA29596.
S34831.
RefSeqiNP_001286908.1. NM_001299979.1.
NP_001286909.1. NM_001299980.1.
NP_476637.1. NM_057289.3.
UniGeneiDm.20322.

Genome annotation databases

EnsemblMetazoaiFBtr0089420; FBpp0088438; FBgn0000109.
FBtr0346440; FBpp0312098; FBgn0000109.
FBtr0346441; FBpp0312099; FBgn0000109.
GeneIDi48224.
KEGGidme:Dmel_CG18315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18432 mRNA. Translation: AAA28377.1.
L06280 Genomic DNA. Translation: AAA57204.1.
AE014296 Genomic DNA. Translation: AAF47589.1.
AY119066 mRNA. Translation: AAM50926.1.
PIRiA29596.
S34831.
RefSeqiNP_001286908.1. NM_001299979.1.
NP_001286909.1. NM_001299980.1.
NP_476637.1. NM_057289.3.
UniGeneiDm.20322.

3D structure databases

ProteinModelPortaliP12426.
SMRiP12426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71230. 7 interactors.
DIPiDIP-22863N.
IntActiP12426. 1 interactor.
MINTiMINT-979410.
STRINGi7227.FBpp0088438.

Proteomic databases

PaxDbiP12426.
PRIDEiP12426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089420; FBpp0088438; FBgn0000109.
FBtr0346440; FBpp0312098; FBgn0000109.
FBtr0346441; FBpp0312099; FBgn0000109.
GeneIDi48224.
KEGGidme:Dmel_CG18315.

Organism-specific databases

CTDi353.
FlyBaseiFBgn0000109. Aprt.

Phylogenomic databases

eggNOGiKOG1712. Eukaryota.
COG0503. LUCA.
InParanoidiP12426.
KOiK00759.
OMAiKKHIGEY.
OrthoDBiEOG091G0XZC.
PhylomeDBiP12426.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00646.
ReactomeiR-DME-6798695. Neutrophil degranulation.
R-DME-74217. Purine salvage.

Miscellaneous databases

GenomeRNAii48224.
PROiP12426.

Gene expression databases

BgeeiFBgn0000109.
GenevisibleiP12426. DM.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr. 1 hit.
InterProiView protein in InterPro
IPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
PROSITEiView protein in PROSITE
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPT_DROME
AccessioniPrimary (citable) accession number: P12426
Secondary accession number(s): Q9W069
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 9, 2003
Last modified: March 15, 2017
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.