P12421 (AROA_BORPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BP0948

Organism

Bordetella pertussis

Taxonomic identifier

520 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer.
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088229
Experimental info
	Sequence conflict	406	1	S → L in AAA22968. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12421 [UniParc]. Last modified September 19, 2003. Version 2. Checksum: D81088B37E2E42C5	FASTA	442	46,662
	10 20 30 40 50 60 MSGLAYLDLP AARLARGEVA LPGSKSISNR VLLLAALAEG STEITGLLDS DDTRVMLAAL 70 80 90 100 110 120 RQLGVSVGEV ADGCVTIEGV ARFPTEQAEL FLGNAGTAFR PLTAALALMG GDYRLSGVPR 130 140 150 160 170 180 MHERPIGDLV DALRQFGAGI EYLGQAGYPP LRIGGGSIRV DGPVRVEGSV SSQFLTALLM 190 200 210 220 230 240 AAPVLARRSG QDITIEVVGE LISKPYIEIT LNLMARFGVS VRRDGWRAFT IARDAVYRGP 250 260 270 280 290 300 GRMAIEGDAS TASYFLALGA IGGGPVRVTG VGEDSIQGDV AFAATLAAMG ADVRYGPGWI 310 320 330 340 350 360 ETRGVRVAEG GRLKAFDADF NLIPDAAMTA ATLALYADGP CRLRNIGSWR VKETDRIHAM 370 380 390 400 410 420 HTELEKLGAG VQSGADWLEV APPEPGGWRD AHIGTWDDHR MAMCFSLAAF GPAAVRILDP 430 440 GCVSKTFPDY FDVYAGLLAA RD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis." Maskell D.J., Morrissey P., Dougan G. J. Bacteriol. 170:2467-2471(1988) [PubMed: 2897356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20023 Genomic DNA. Translation: AAA22968.1. BX640413 Genomic DNA. Translation: CAE41250.1.
PIR	XUBRVS. A32007.
RefSeq	NP_879749.1. NC_002929.2.
3D structure databases
ProteinModelPortal	P12421.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2664265.
GenomeReviews	Gene locus BP0948 in contig BX470248_GR.
KEGG	bpe:BP0948.
NMPDR	fig|257313.1.peg.828.
PATRIC	21155018. VBIBorPer7866_1012.
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
PhylomeDB	P12421.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	BPER257313:BP0948-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BORPE

Accession

Primary (citable) accession number: P12421

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	September 19, 2003
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program