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P12419

- MUNS_REOVD

UniProt

P12419 - MUNS_REOVD

Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 2 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein mu-NS
    Short name:
    MuNS
    Gene namesi
    Name:M3
    OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
    Taxonomic identifieri10886 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006373: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Protein mu-NSPRO_0000222748Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Interactioni

    Subunit structurei

    Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 1313Interaction with sigma-NSBy similarityAdd
    BLAST
    Regioni14 – 4027Interaction with mu-2By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili522 – 55938Sequence AnalysisAdd
    BLAST
    Coiled coili628 – 68457Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the orthoreovirus mu-NS protein family.Curated

    Keywords - Domaini

    Coiled coil

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform mu-NS (identifier: P12419-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK    50
    AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL 100
    ECLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM 150
    LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ 200
    LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA 250
    ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF 300
    KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD 350
    LYANTQVISI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG 400
    VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL 450
    MCYYHPSRKL AYGEVLFLER SNDVTDGIKL QLDASRQCHE CPVLQQKVVE 500
    LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME LSRAQSLNAQ 550
    LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR 600
    SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ 650
    IAELVSTITQ NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT 700
    PIDSVDDVAD LIDFSVPTDE L 721
    Length:721
    Mass (Da):80,158
    Last modified:July 22, 2008 - v2
    Checksum:i8E93017633E95940
    GO
    Isoform mu-NSC (identifier: P12419-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-40: Missing.

    Note: It is unsure whether Met-41 is the initiator.

    Show »
    Length:681
    Mass (Da):76,019
    Checksum:i2DBC137D8E35941A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491T → I in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti127 – 1271Missing in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti180 – 1801E → K in ABP48918. (PubMed:18005692)Curated
    Sequence conflicti201 – 2022LV → QL in ABP48918. (PubMed:18005692)Curated
    Sequence conflicti201 – 2011L → H in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti274 – 2741D → H in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti302 – 3021L → R in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti429 – 4291T → N in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti587 – 5871Missing in AAA47260. (PubMed:2523177)Curated
    Sequence conflicti705 – 7073VDD → ADG in ABP48918. (PubMed:18005692)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4040Missing in isoform mu-NSC. CuratedVSP_034862Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27262 Genomic RNA. Translation: AAA47260.1.
    AF174384 Genomic RNA. Translation: AAF13171.1.
    EF494440 Genomic RNA. Translation: ABP48918.1.
    PIRiB30179. MNXR3D.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27262 Genomic RNA. Translation: AAA47260.1 .
    AF174384 Genomic RNA. Translation: AAF13171.1 .
    EF494440 Genomic RNA. Translation: ABP48918.1 .
    PIRi B30179. MNXR3D.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
      Wiener J.R., Bartlett J.A., Joklik W.K.
      Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
    2. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
      McCutcheon A.M., Broering T.J., Nibert M.L.
      Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Infectious clone.

    Entry informationi

    Entry nameiMUNS_REOVD
    AccessioniPrimary (citable) accession number: P12419
    Secondary accession number(s): A4ZY25, Q9PY81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3