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P12419 (MUNS_REOVD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein mu-NS

Short name=MuNS
Gene names
Name:M3
OrganismReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]
Taxonomic identifier10886 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions By similarity.

Subunit structure

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions By similarity.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the orthoreovirus mu-NS protein family.

Ontologies

Keywords
   Coding sequence diversityAlternative initiation
   DomainCoiled coil
   LigandRNA-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform mu-NS (identifier: P12419-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform mu-NSC (identifier: P12419-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.
Note: It is unsure whether Met-41 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Protein mu-NS
PRO_0000222748

Regions

Region1 – 1313Interaction with sigma-NS By similarity
Region14 – 4027Interaction with mu-2 By similarity
Coiled coil522 – 55938 Potential
Coiled coil628 – 68457 Potential

Natural variations

Alternative sequence1 – 4040Missing in isoform mu-NSC.
VSP_034862

Experimental info

Sequence conflict491T → I in AAA47260. Ref.1
Sequence conflict1271Missing in AAA47260. Ref.1
Sequence conflict1801E → K in ABP48918. Ref.3
Sequence conflict201 – 2022LV → QL in ABP48918. Ref.3
Sequence conflict2011L → H in AAA47260. Ref.1
Sequence conflict2741D → H in AAA47260. Ref.1
Sequence conflict3021L → R in AAA47260. Ref.1
Sequence conflict4291T → N in AAA47260. Ref.1
Sequence conflict5871Missing in AAA47260. Ref.1
Sequence conflict705 – 7073VDD → ADG in ABP48918. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform mu-NS [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: 8E93017633E95940

FASTA72180,158
        10         20         30         40         50         60 
MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK AIEQGSMSIP 

        70         80         90        100        110        120 
YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL ECLPQLEQAM AFVASPESFQ 

       130        140        150        160        170        180 
ASDVAKRFAI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIVAEIERL ASTSVSVRTE 

       190        200        210        220        230        240 
EAKVADEELM LFGLDHRGPQ LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR 

       250        260        270        280        290        300 
FMFMQHKPLA ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF 

       310        320        330        340        350        360 
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD LYANTQVISI 

       370        380        390        400        410        420 
TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG VSLCALLMDE KLSVLNSACR 

       430        440        450        460        470        480 
MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL MCYYHPSRKL AYGEVLFLER SNDVTDGIKL 

       490        500        510        520        530        540 
QLDASRQCHE CPVLQQKVVE LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME 

       550        560        570        580        590        600 
LSRAQSLNAQ LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR 

       610        620        630        640        650        660 
SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ IAELVSTITQ 

       670        680        690        700        710        720 
NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT PIDSVDDVAD LIDFSVPTDE 


L 

« Hide

Isoform mu-NSC [UniParc].

Checksum: 2DBC137D8E35941A
Show »

FASTA68176,019

References

[1]"The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
Wiener J.R., Bartlett J.A., Joklik W.K.
Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[2]"Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
McCutcheon A.M., Broering T.J., Nibert M.L.
Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[3]"A plasmid-based reverse genetics system for animal double-stranded RNA viruses."
Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S.
Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Infectious clone.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27262 Genomic RNA. Translation: AAA47260.1.
AF174384 Genomic RNA. Translation: AAF13171.1.
EF494440 Genomic RNA. Translation: ABP48918.1.
PIRMNXR3D. B30179.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameMUNS_REOVD
AccessionPrimary (citable) accession number: P12419
Secondary accession number(s): A4ZY25, Q9PY81
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 22, 2008
Last modified: April 3, 2013
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families