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Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mu-NS
Short name:
MuNS
Gene namesi
Name:M3
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006373 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Protein mu-NSPRO_0000222748Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1313Interaction with sigma-NSBy similarityAdd
BLAST
Regioni14 – 4027Interaction with mu-2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili522 – 55938Sequence AnalysisAdd
BLAST
Coiled coili628 – 68457Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the orthoreovirus mu-NS protein family.Curated

Keywords - Domaini

Coiled coil

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform mu-NS (identifier: P12419-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK
60 70 80 90 100
AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL
110 120 130 140 150
ECLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM
160 170 180 190 200
LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ
210 220 230 240 250
LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA
260 270 280 290 300
ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF
310 320 330 340 350
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD
360 370 380 390 400
LYANTQVISI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG
410 420 430 440 450
VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL
460 470 480 490 500
MCYYHPSRKL AYGEVLFLER SNDVTDGIKL QLDASRQCHE CPVLQQKVVE
510 520 530 540 550
LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME LSRAQSLNAQ
560 570 580 590 600
LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR
610 620 630 640 650
SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ
660 670 680 690 700
IAELVSTITQ NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT
710 720
PIDSVDDVAD LIDFSVPTDE L
Length:721
Mass (Da):80,158
Last modified:July 22, 2008 - v2
Checksum:i8E93017633E95940
GO
Isoform mu-NSC (identifier: P12419-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.
Show »
Length:681
Mass (Da):76,019
Checksum:i2DBC137D8E35941A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491T → I in AAA47260 (PubMed:2523177).Curated
Sequence conflicti127 – 1271Missing in AAA47260 (PubMed:2523177).Curated
Sequence conflicti180 – 1801E → K in ABP48918 (PubMed:18005692).Curated
Sequence conflicti201 – 2022LV → QL in ABP48918 (PubMed:18005692).Curated
Sequence conflicti201 – 2011L → H in AAA47260 (PubMed:2523177).Curated
Sequence conflicti274 – 2741D → H in AAA47260 (PubMed:2523177).Curated
Sequence conflicti302 – 3021L → R in AAA47260 (PubMed:2523177).Curated
Sequence conflicti429 – 4291T → N in AAA47260 (PubMed:2523177).Curated
Sequence conflicti587 – 5871Missing in AAA47260 (PubMed:2523177).Curated
Sequence conflicti705 – 7073VDD → ADG in ABP48918 (PubMed:18005692).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform mu-NSC. CuratedVSP_034862Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27262 Genomic RNA. Translation: AAA47260.1.
AF174384 Genomic RNA. Translation: AAF13171.1.
EF494440 Genomic RNA. Translation: ABP48918.1.
PIRiB30179. MNXR3D.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27262 Genomic RNA. Translation: AAA47260.1.
AF174384 Genomic RNA. Translation: AAF13171.1.
EF494440 Genomic RNA. Translation: ABP48918.1.
PIRiB30179. MNXR3D.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
    Wiener J.R., Bartlett J.A., Joklik W.K.
    Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  2. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
    McCutcheon A.M., Broering T.J., Nibert M.L.
    Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Infectious clone.

Entry informationi

Entry nameiMUNS_REOVD
AccessioniPrimary (citable) accession number: P12419
Secondary accession number(s): A4ZY25, Q9PY81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 22, 2008
Last modified: January 7, 2015
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.