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P12419

- MUNS_REOVD

UniProt

P12419 - MUNS_REOVD

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Protein

Protein mu-NS

Gene
M3
Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mu-NS
Short name:
MuNS
Gene namesi
Name:M3
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006373: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Protein mu-NSPRO_0000222748Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1313Interaction with sigma-NS By similarityAdd
BLAST
Regioni14 – 4027Interaction with mu-2 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili522 – 55938 Reviewed predictionAdd
BLAST
Coiled coili628 – 68457 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform mu-NS (identifier: P12419-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK    50
AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL 100
ECLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM 150
LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ 200
LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA 250
ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF 300
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD 350
LYANTQVISI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG 400
VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL 450
MCYYHPSRKL AYGEVLFLER SNDVTDGIKL QLDASRQCHE CPVLQQKVVE 500
LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME LSRAQSLNAQ 550
LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR 600
SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ 650
IAELVSTITQ NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT 700
PIDSVDDVAD LIDFSVPTDE L 721
Length:721
Mass (Da):80,158
Last modified:July 22, 2008 - v2
Checksum:i8E93017633E95940
GO
Isoform mu-NSC (identifier: P12419-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.

Show »
Length:681
Mass (Da):76,019
Checksum:i2DBC137D8E35941A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform mu-NSC. VSP_034862Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491T → I in AAA47260. 1 Publication
Sequence conflicti127 – 1271Missing in AAA47260. 1 Publication
Sequence conflicti180 – 1801E → K in ABP48918. 1 Publication
Sequence conflicti201 – 2022LV → QL in ABP48918. 1 Publication
Sequence conflicti201 – 2011L → H in AAA47260. 1 Publication
Sequence conflicti274 – 2741D → H in AAA47260. 1 Publication
Sequence conflicti302 – 3021L → R in AAA47260. 1 Publication
Sequence conflicti429 – 4291T → N in AAA47260. 1 Publication
Sequence conflicti587 – 5871Missing in AAA47260. 1 Publication
Sequence conflicti705 – 7073VDD → ADG in ABP48918. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27262 Genomic RNA. Translation: AAA47260.1.
AF174384 Genomic RNA. Translation: AAF13171.1.
EF494440 Genomic RNA. Translation: ABP48918.1.
PIRiB30179. MNXR3D.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27262 Genomic RNA. Translation: AAA47260.1 .
AF174384 Genomic RNA. Translation: AAF13171.1 .
EF494440 Genomic RNA. Translation: ABP48918.1 .
PIRi B30179. MNXR3D.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
    Wiener J.R., Bartlett J.A., Joklik W.K.
    Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  2. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
    McCutcheon A.M., Broering T.J., Nibert M.L.
    Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Infectious clone.

Entry informationi

Entry nameiMUNS_REOVD
AccessioniPrimary (citable) accession number: P12419
Secondary accession number(s): A4ZY25, Q9PY81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 22, 2008
Last modified: July 9, 2014
This is version 43 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi