P12419 (MUNS_REOVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein mu-NS
Short name=MuNS

Gene names

Name:

M3

Organism

Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]

Taxonomic identifier

10886 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 3

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions By similarity.
Subunit structure	Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the orthoreovirus mu-NS protein family.

Ontologies

Keywords
Coding sequence diversity	Alternative initiation
Domain	Coiled coil
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform mu-NS (identifier: P12419-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform mu-NSC (identifier: P12419-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Protein mu-NS		PRO_0000222748
Regions
	Region	1 – 13	13	Interaction with sigma-NS By similarity
	Region	14 – 40	27	Interaction with mu-2 By similarity
	Coiled coil	522 – 559	38	Potential
	Coiled coil	628 – 684	57	Potential
Natural variations
	Alternative sequence	1 – 40	40	Missing in isoform mu-NSC.		VSP_034862
Experimental info
	Sequence conflict	49	1	T → I in AAA47260. Ref.1
	Sequence conflict	127	1	Missing in AAA47260. Ref.1
	Sequence conflict	180	1	E → K in ABP48918. Ref.3
	Sequence conflict	201 – 202	2	LV → QL in ABP48918. Ref.3
	Sequence conflict	201	1	L → H in AAA47260. Ref.1
	Sequence conflict	274	1	D → H in AAA47260. Ref.1
	Sequence conflict	302	1	L → R in AAA47260. Ref.1
	Sequence conflict	429	1	T → N in AAA47260. Ref.1
	Sequence conflict	587	1	Missing in AAA47260. Ref.1
	Sequence conflict	705 – 707	3	VDD → ADG in ABP48918. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform mu-NS [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 8E93017633E95940	FASTA	721	80,158
	10 20 30 40 50 60 MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK AIEQGSMSIP 70 80 90 100 110 120 YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL ECLPQLEQAM AFVASPESFQ 130 140 150 160 170 180 ASDVAKRFAI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIVAEIERL ASTSVSVRTE 190 200 210 220 230 240 EAKVADEELM LFGLDHRGPQ LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR 250 260 270 280 290 300 FMFMQHKPLA ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF 310 320 330 340 350 360 KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD LYANTQVISI 370 380 390 400 410 420 TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG VSLCALLMDE KLSVLNSACR 430 440 450 460 470 480 MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL MCYYHPSRKL AYGEVLFLER SNDVTDGIKL 490 500 510 520 530 540 QLDASRQCHE CPVLQQKVVE LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME 550 560 570 580 590 600 LSRAQSLNAQ LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR 610 620 630 640 650 660 SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ IAELVSTITQ 670 680 690 700 710 720 NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT PIDSVDDVAD LIDFSVPTDE L
	Isoform mu-NSC [UniParc]. Checksum: 2DBC137D8E35941A	FASTA	681	76,019
	10 20 30 40 50 60 MSQSREFLTK AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL 70 80 90 100 110 120 ECLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF 130 140 150 160 170 180 DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ LVDVSDAKGI MKAADIQTTH 190 200 210 220 230 240 DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA ADQSYFTLET ADYFKIYPTY DEHDGRMADQ 250 260 270 280 290 300 KQSGLILCTK DEVLAEQTIF KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ 310 320 330 340 350 360 RSGQRSVLDD LYANTQVISI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG 370 380 390 400 410 420 VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL MCYYHPSRKL 430 440 450 460 470 480 AYGEVLFLER SNDVTDGIKL QLDASRQCHE CPVLQQKVVE LEKQIIMQKS IQSDPTPVAL 490 500 510 520 530 540 QPLLSQLREL SSEVTRLQME LSRAQSLNAQ LEADVKSAQS CSLDMYLRHH TCINGHAKED 550 560 570 580 590 600 ELLDAVRVAP DVRREIMEKR SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL 610 620 630 640 650 660 RDSAEKYEKQ IAELVSTITQ NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT 670 680 PIDSVDDVAD LIDFSVPTDE L

References

[1]	"The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively." Wiener J.R., Bartlett J.A., Joklik W.K. Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[2]	"Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein." McCutcheon A.M., Broering T.J., Nibert M.L. Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[3]	"A plasmid-based reverse genetics system for animal double-stranded RNA viruses." Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S. Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Infectious clone.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27262 Genomic RNA. Translation: AAA47260.1. AF174384 Genomic RNA. Translation: AAF13171.1. EF494440 Genomic RNA. Translation: ABP48918.1.
PIR	MNXR3D. B30179.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MUNS_REOVD

Accession

Primary (citable) accession number: P12419
Secondary accession number(s): A4ZY25, Q9PY81

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 22, 2008
Last modified:	April 3, 2013
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program