Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mu-NS
Short name:
MuNS
Gene namesi
Name:M3
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
Proteomesi
  • UP000006373 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002227481 – 721Protein mu-NSAdd BLAST721

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 13Interaction with sigma-NSBy similarityAdd BLAST13
Regioni14 – 40Interaction with mu-2By similarityAdd BLAST27

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili522 – 559Sequence analysisAdd BLAST38
Coiled coili628 – 684Sequence analysisAdd BLAST57

Sequence similaritiesi

Belongs to the orthoreovirus mu-NS protein family.Curated

Keywords - Domaini

Coiled coil

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform mu-NS (identifier: P12419-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK
60 70 80 90 100
AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL
110 120 130 140 150
ECLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM
160 170 180 190 200
LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ
210 220 230 240 250
LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA
260 270 280 290 300
ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF
310 320 330 340 350
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD
360 370 380 390 400
LYANTQVISI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG
410 420 430 440 450
VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL
460 470 480 490 500
MCYYHPSRKL AYGEVLFLER SNDVTDGIKL QLDASRQCHE CPVLQQKVVE
510 520 530 540 550
LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME LSRAQSLNAQ
560 570 580 590 600
LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR
610 620 630 640 650
SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ
660 670 680 690 700
IAELVSTITQ NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT
710 720
PIDSVDDVAD LIDFSVPTDE L
Length:721
Mass (Da):80,158
Last modified:July 22, 2008 - v2
Checksum:i8E93017633E95940
GO
Isoform mu-NSC (identifier: P12419-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.
Show »
Length:681
Mass (Da):76,019
Checksum:i2DBC137D8E35941A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49T → I in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti127Missing in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti180E → K in ABP48918 (PubMed:18005692).Curated1
Sequence conflicti201 – 202LV → QL in ABP48918 (PubMed:18005692).Curated2
Sequence conflicti201L → H in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti274D → H in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti302L → R in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti429T → N in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti587Missing in AAA47260 (PubMed:2523177).Curated1
Sequence conflicti705 – 707VDD → ADG in ABP48918 (PubMed:18005692).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0348621 – 40Missing in isoform mu-NSC. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27262 Genomic RNA. Translation: AAA47260.1.
AF174384 Genomic RNA. Translation: AAF13171.1.
EF494440 Genomic RNA. Translation: ABP48918.1.
PIRiB30179. MNXR3D.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27262 Genomic RNA. Translation: AAA47260.1.
AF174384 Genomic RNA. Translation: AAF13171.1.
EF494440 Genomic RNA. Translation: ABP48918.1.
PIRiB30179. MNXR3D.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiMUNS_REOVD
AccessioniPrimary (citable) accession number: P12419
Secondary accession number(s): A4ZY25, Q9PY81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 22, 2008
Last modified: November 11, 2015
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.